Claims
- 1. A method for processing a biological material comprising:
placing a removable member on a base plate for establishing self-sealing contact of the removable member on the base plate, the removable member being adapted to repeatedly self-seal on the base plate, further defining a plurality of orifices therein and having walls bounding the plurality of orifices at sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of wells therewith corresponding to respective ones of the plurality of orifices; immobilizing a biological material on regions of the base plate exposed by the plurality of orifices; removing the removable member from the base plate such that the biological material is arrayed on the base plate in an array that corresponds to an array defined by the plurality of orifices; and simultaneously performing at least one bulk process on the array of biological material on the base plate.
- 2. The method of claim 1, wherein simultaneously performing includes washing the array of biological material on the base plate.
- 3. The method of claim 1, wherein simultaneously performing includes incubating the array of biological material on the base plate with a reagent.
- 4. The method of claim 1, wherein simultaneously performing includes fixing the array of biological material on the base plate.
- 5. The method of claim 1, wherein simultaneously performing includes developing the array of biological material.
- 6. The method of claim 1, further comprising processing the biological material prior to immobilizing.
- 7. The method of claim 1, wherein the removable member is a first removable member, the plurality of orifices is a plurality of first orifices, and the plurality of wells is a plurality of first wells, the method further comprising placing a second removable member on the base plate for establishing self-sealing contact of the second removable member on the base plate, the second removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the second removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of second wells therewith, the second wells being arrayed such that the array of biological material on the base plate is within respective ones of the plurality of second wells.
- 8. The method of claim 7, further comprising processing the array of biological material in the second wells.
- 9. A method for processing a biological material comprising:
placing a first removable member on a base plate for establishing self-sealing contact of the first removable member on the base plate, the first removable member being adapted to repeatedly self-seal on the base plate, further defining a plurality of first orifices therein and having walls bounding the plurality of first orifices at sides of the first orifices, the first removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of first wells therewith corresponding to respective ones of the plurality of first orifices; immobilizing a biological material on regions of the base plate exposed by the plurality of first orifices; removing the first removable member from the base plate such that the biological material is arrayed on the base plate in a first array that corresponds to an array defined by the plurality of first orifices; and placing a second removable member on the base plate for establishing self-sealing contact of the second removable member on the base plate, the second removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the second removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of second wells therewith, the plurality of second orifices further being configured such that each region on the base plate exposed by a respective one of the second plurality of orifices is smaller than each corresponding region on the base plate exposed by a respective one of the first plurality of orifices, the second wells being arrayed such that the first array of biological material on the base plate is within respective ones of the plurality of second wells.
- 10. The method of claim 9, further comprising processing the array of biological material in the plurality of first wells before removing the first removable member
- 11. The method of claim 10, wherein processing the array comprises exposing the array of biological material to at least one of a biomolecule, a reagent, an enzyme or an antibody.
- 12. The method of claim 10, wherein processing includes at least one of washing the array of biological material, fixing the array of biological material and developing the array of biological material.
- 13. The method of claim 9, further comprising processing the array of biological material in each of the plurality of second wells.
- 14. The method of claim 13, wherein processing comprises exposing the array of biological material to at least one of a biomolecule, a reagent, an enzyme or an antibody.
- 15. The method of claim 13, wherein processing includes at least one of washing the array of biological material, fixing the array of biological material and developing the array of biological material.
- 16. A method for processing a biological material comprising:
placing a first removable member on a base plate for establishing self-sealing contact of the first removable member on the base plate, the first removable member being adapted to repeatedly self-seal on the base plate, further defining a plurality of first orifices therein and having walls bounding the plurality of first orifices at sides of the first orifices, the first removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of first wells therewith corresponding to respective ones of the plurality of first orifices; immobilizing a biological material on regions of the base plate exposed by the plurality of first orifices; and placing a second removable member on an upper surface of the first removable member for establishing self-sealing contact of the second removable member on the first removable member, the second removable member being adapted to repeatedly self seal on the first removable member, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the second removable member further being configured such that, when it is placed in self-sealing contact with the first removable member, it defines a plurality of second wells therewith, the second wells being arrayed such that the first array of biological material is at least partially within respective ones of the plurality of second wells; and processing the array of biological material.
- 17. A method for processing a biological material comprising:
immobilizing a biological material on a base plate for forming an array of the biological material on the base plate; placing a removable member on the base plate for establishing self-sealing contact of the removable member on the base plate, the removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of orifices therein and having walls bounding respective ones of the plurality of orifices on sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of wells therewith, the wells being arrayed such that the array of biological material is at least partially within respective ones of the plurality of wells.
- 18. A method for processing a biological material comprising:
placing a removable member on a base plate for establishing self-sealing contact of the removable member on the base plate, the removable member being adapted to repeatedly self-seal on the base plate, further defining a plurality of orifices therein and having walls bounding the plurality of orifices at sides of the orifices, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of wells therewith corresponding to respective ones of the plurality of orifices; immobilizing a biological material on regions of the base plate exposed by the plurality of orifices and corresponding to respective ones of the plurality of wells; processing the biological material on the base plate in the plurality of wells; removing the removable member from the base plate such that the biological material is arrayed on the base plate in an array that corresponds to an array defined by the plurality of orifices; and detecting a change in at least one immobilized biological material resulting from processing.
- 19. The method of claim 18, wherein the removable member is a first removable member, the plurality of orifices is a plurality of first orifices, the plurality of wells is a plurality of first wells, the method further comprising placing a second removable member on the base plate for establishing self-sealing contact of the second removable member on the base plate, the second removable member being adapted to repeatedly self seal on the base plate, further defining a plurality of second orifices therein and having walls bounding respective ones of the plurality of second orifices on sides of the orifices, the second removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines a plurality of second wells therewith, the second wells being arrayed such that the array of biological material on the base plate is within respective ones of the plurality of second wells; and
processing the biological material in the plurality of second wells.
- 20. The method of claim 1, further comprising treating the base plate to enhance immobilization of kinase substrates thereon.
- 21. The method of claim 20, wherein treating comprises treating the base plate with a solution of mixed self-assembled monoloayer (SAM), such that binding of kinase substrates is enhanced in discrete areas and reduced or eliminated in others.
- 22. The method of claim 21, wherein treating comprises treating the base plate with a solution of mixed SAM, such that specific binding of kinase substrates in desired orientations is enhanced and non-specific binding of kinase substrates is reduced or eliminated.
- 23. The method of claim 20, wherein treating comprises treating the base plate with mixed SAM comprising about 0.1 to 20% maleimide-terminal groups in a background of tri(ethylene glycol) terminal groups.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US01/50909 |
Nov 2001 |
WO |
|
Parent Case Info
[0001] This application claims the benefit of priority of U.S. provisional applications Nos. 60/307,839 (filed Jul. 27, 2001), 60/323,743 (filed Sep. 21, 2001), 60/357,649 (filed 20, 2002), 60/366,260 (filed Mar. 22, 2002), 60/375,024 (filed Apr. 25, 2002), 60/380,314 (filed May 15, 2002), and PCT/US01/50909 (filed Nov. 7, 2001), and these applications are hereby incorporated by reference herein.
Provisional Applications (6)
|
Number |
Date |
Country |
|
60307839 |
Jul 2001 |
US |
|
60323743 |
Sep 2001 |
US |
|
60357649 |
Feb 2002 |
US |
|
60366260 |
Mar 2002 |
US |
|
60375024 |
Apr 2002 |
US |
|
60380314 |
May 2002 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09709776 |
Nov 2000 |
US |
Child |
10206590 |
Jul 2002 |
US |