Claims
- 1. A method for producing an end-product comprising the steps of:
a) contacting a carbon substrate and at least one substrate-converting enzyme to produce an intermediate; and b) contacting said intermediate with at least one intermediate-converting enzyme, wherein said intermediate is substantially all converted by said intermediate enzyme to said end-product.
- 2. The method of claim 1, wherein said intermediate-converting enzyme is a microbial enzyme.
- 3. The method of claim 2, wherein said intermediate-converting microbial enzyme is secreted by a microorganism in contact with said intermediate.
- 4. The method of claim 1, wherein said substrate-converting enzyme is a microbial enzyme.
- 5. The method of claim 4, wherein said substrate-converting microbial enzyme is secreted by a microorganism in contact with said substrate.
- 6. The method of claim 1, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the same species.
- 7. The method of claim 1, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the different species.
- 8. The method of claim 1, wherein concentration level of said intermediate is maintained at a level below that which triggers catabolite repression effects upon the conversion of said intermediate to said end-product.
- 9. The method of claim 1, wherein concentration level of said intermediate is maintained at a level below that which triggers enzymatic inhibition effects upon the conversion of said intermediate to said end-product.
- 10. The method of claim 1, wherein said intermediate is converted to said end-product at a rate sufficient to maintain the concentration of said at less than 0.25%.
- 11. The method of claim 1, wherein said substrate is selected from the group consisting of biomass and starch.
- 12. The method of claim 1, wherein said intermediate is selected from the group consisting of hexoses and pentoses.
- 13. The method of claim 12, wherein said hexose is glucose.
- 14. The method of claim 1 wherein said end-product is selected from the group consisting of 1,3-propanediol, gluconic acid, glycerol, succinic acid, lactic acid, 2,5-diketo-D-gluconic acid, gluconate, glucose, alcohol, and ascorbic acid intermediates.
- 15. The method of claim 1, wherein said contacting said substrate and substrate-converting enzyme further comprises bioconverting said substrate to produce said intermediate.
- 16. A method for producing an end-product comprising the steps of:
a) contacting a carbon substrate and at least one substrate-converting enzyme to produce an intermediate; and b) contacting said intermediate with at least one intermediate-converting enzyme, wherein said intermediate is substantially all converted by said intermediate enzyme to said end-product, and wherein the presence of said end-product does not inhibit the further production of said end-product.
- 17. The method of claim 16, wherein said intermediate-converting enzyme is a microbial enzyme.
- 18. The method of claim 16, wherein said intermediate-converting microbial enzyme is secreted by a microorganism in contact with said intermediate.
- 19. The method of claim 16, wherein said substrate-converting enzyme is a microbial enzyme.
- 20. The method of claim 16, wherein said substrate-converting microbial enzyme is secreted by a microorganism in contact with said substrate.
- 21. The method of claim 16, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the same species.
- 22. The method of claim 16, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the different species.
- 23. A method for producing an end-product comprising the steps of:
a) contacting a carbon substrate and at least one substrate-converting enzyme to produce an intermediate; and b) contacting said intermediate with at least one intermediate-converting enzyme, wherein said intermediate is substantially all converted by said intermediate enzyme to said end-product, and wherein the presence of said substrate does not inhibit the further production of said end-product.
- 24. The method of claim 23, wherein said intermediate-converting enzyme is a microbial enzyme.
- 25. The method of claim 23, wherein said intermediate-converting microbial enzyme is secreted by a microorganism in contact with said intermediate.
- 26. The method of claim 23, wherein said substrate-converting enzyme is a microbial enzyme.
- 27. The method of claim 23, wherein said substrate-converting microbial enzyme is produced is secreted by a microorganism in contact with said substrate.
- 28. The method of claim 23, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the same species.
- 29. The method of claim 23, wherein said intermediate-converting enzyme and said substrate-converting enzyme are produced by microorganisms of the different species.
Parent Case Info
[0001] The present application claims priority to U.S. Prov. Patent Appln. Ser. No. 60/355,260, filed Feb. 8, 2002, as well as U.S. Prov. Patent Appln. Ser. No. 60/355,180, filed Feb. 8, 2002.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60355260 |
Feb 2002 |
US |
|
60355180 |
Feb 2002 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
10359771 |
Feb 2003 |
US |
Child |
10765652 |
Jan 2004 |
US |