Patent Application
20180243179
The present invention is in the field of dermatology, pharmaceutics and cosmetics. In particular the invention is in the field of the production of pharmaceutically, dermatologically or cosmetically applicable substances and in the use and application thereof.
Biopolymers, such as collagen, polysaccharides or hyaluronic acid are commonly used in cosmetic or dermatological compositions. In many cases these biopolymers are used as moisturizers or antioxidants. Common forms of administration are as cream, serum, patches, masks, balms, liquids or as an ointment.
Hyaluronic acid or hyaluronan for example is a biopolymer, which is widely distributed among the human tissue. It is an anionic, non-sulfated glycosaminoglycan comprising the following structure:
Hyaluronic acid has several medical uses, in particular in dermatology, and is commonly used in cosmetic products, in particular so called anti-ageing products.
In general the bioactivity of biopolymers, such as hyaluronic acid is directly dependent on the average molecular weight of said biopolymers. Taking hyaluronic acid and its use in dermatology for example, the average molecular weight determines the depth of skin penetration and the potential dermatological effects of hyaluronic acid (see
It is known, that the biological functionality of biopolymers is dependent on their average molecular weight, several methods have been developed to generate biopolymers with defined average molecular weight.
EP 2 479 194 A2 describes the hydrolysis of hyaluronic acid on activated charcoal. EP 2 463 309 B1 and EP 1 992 645 A1 describe several methods for the acidic hydrolysation of hyaluronic acid. Other methods involve the use of enzymatic hydrolysis and filtration (EP 0 138 572 B1) or the use of high temperatures and strong shearing forces (EP 1 987 153 B1).
The problem with all these methods is, that in particular hyaluronic acid needs extensive purification steps to remove the low molecular weight hyaluronic acids, which can be pro-inflammatory.
It is therefore necessary to provide a method for the efficient production of pure biopolymers with defined molecular weight distribution, in particular hyaluronic acid, which allows the control of the average molecular weight of the biopolymer and does not need any further additional purification steps.
The present invention relates to a method for the production of a biopolymer composition comprising at least one biopolymer, wherein the at least one biopolymer has a defined average molecular weight and a defined molecular weight distribution, the method comprising
In one embodiment of the invention the biopolymers are biopolymers with high molecular weight. In a preferred embodiment the biopolymers are biopolymers with native high molecular weight.
In a preferred embodiment the invention relates to a method for the production of a biopolymer composition comprising at least one biopolymer, wherein the at least one biopolymer has a defined average molecular weight and a defined weight distribution, the method comprising
In one preferred embodiment of the invention the biopolymers are biopolymers with high molecular weight. In a more preferred embodiment the biopolymers are biopolymers with native high molecular weight.
The invention further relates to the use of said method for the production of biopolymer compositions and to biopolymer compositions, which are produced by said method.
In the context of the present invention, biopolymers are polymers produced by living organisms. The present invention only relates to native high molecular weight biopolymers, which are preferably not technically or chemically modified, besides the common and native modifications, which occur in the living organism. As polymers, they are characterized by repetitive monomeric motives.
In general biopolymers are divided into three main classes: polynucleotides, polypeptides and polysaccharides. Within the context of this invention the term “biopolymer” only refers to polypeptides and polysaccharides. In the present invention the term “biopolymer” encompasses all naturally occurring modifications of biopolymers, e.g. glycosylation, partial hydrolysis or the attachment of lipids to polypeptides.
Polymers consisting of biological units, but not produced in a living organism, such as polylactic acid, are not considered biopolymers within the meaning of the invention. Biopolymers according to the above mentioned definition processed according to the present invention, are biopolymers in the context of the present invention.
Non-limiting examples for biopolymers according to the present invention comprise: collagens, starch, cellulose derivatives, glucosamino glycans, polysaccarides or fucoidanes.
In the context of the present invention a frozen composition refers to a composition, which is in a solid state of matter, regardless of its state of matter at 25° C. In most embodiments a frozen composition is a composition in a liquid state of matter at 25° C., which has been cooled down to a solid state of matter. In one embodiment cooling is done by shock-freezing in liquid nitrogen. Alternative ways of cooling are cooling and freezing in a freezer. In a preferred embodiment freezing is done at a temperature between −50° C. and −4° C.
In the context of the present invention lyophilization or lyophilizing refers to a dehydration process, wherein water is removed by sublimation. Lyophilization is commonly referred to as freeze drying. In general lyophilization comprises three stages:
In one embodiment of the invention, the temperature is controlled during the second drying phase. In another embodiment the temperature is controlled during the primary drying phase. In a particular embodiment the composition is dried using only one drying step, wherein the conditions correspond to the conditions of the second drying step. In an alternative embodiment the composition is dried using only one drying step, wherein the conditions correspond to the conditions of the first drying step.
Within the meaning of the present invention the “temperature during the sublimation process” refers to the temperature of the storage plate on which the composition is placed.
In the context of the present invention the term aqueous solution refers to a solution, wherein the solvent is water. Within the context of the present invention the term further refers to coarse or colloidal suspensions of components, for example non-water-soluble biopolymers or non-soluble cosmetic additions in water.
In the context of the present invention the term emulsion refers to mixtures of normally immiscible liquids. In the context of the present invention the term emulsion in particular refers to water-in-oil or oil-in-water emulsion. Preferably in the context of the present invention the emulsion is stabilized by the use of an emulsifying agent or emulsifier. Non-limited examples for emulsifying agents are lecithin, sodium stearoyl lactylate, polymers with emulsifying functionalities or detergents.
In the context of the present invention a reaction vessel is any suitable vessel for containing and processing the compositions. Preferably said vessel is suitable for freezing and lyophilization processes.
The inventors found surprisingly, that biopolymers can be subjected to controlled degradation during lyophilization processes, resulting in biopolymers with defined average molecular weight. In addition the inventors found, that the molecular weight distribution of the degraded biopolymer can be influenced by combining frozen compositions during the lyophilization process.
A first aspect of the present invention relates to a method for the production of a biopolymer composition comprising at least one biopolymer, wherein the at least one biopolymer has a defined average molecular weight and a defined weight distribution, the method comprising
Combining the compositions without substantially mixing the compostions refers to a process, wherein the compositions are combined in one reaction vessel but retain individual concentrations of compounds and other properties, such as pH value. This could be done by providing and combining two frozen compositions or by combining two compositions with high viscosity by overlaying the compositions and freezing them prior to lyophilization.
In a preferred embodiment the compositions are frozen and then combined in one reaction vessel.
In a preferred embodiment the biopolymers are biopolymers with high molecular weight. In a more preferred embodiment the biopolymers are biopolymers with native high molecular weight.
The compositions comprising a biopolymer can be any kind of composition, provided said compositions comprise at least small amounts of water in addition to said biopolymer. Said composition may comprise additional biopolymers, i.e. mixtures.
The first and second compositions might comprise different biopolymers. In a preferred embodiment both compositions comprise the same biopolymer. In another preferred embodiment the first and second composition comprise only one biopolymer each.
In a preferred embodiment the biopolymers are biopolymers with high molecular weight. In a more preferred embodiment the biopolymers are biopolymers with native high molecular weight.
The method is in particular suitable for biopolymers selected from the group comprising hyaluronic acid, collagen, glucosamino glycans, polysaccharides and fucoidanes. In a preferred embodiment the biopolymer is a glucosamino glycan or polysaccarid. In a more preferred embodiment the biopolymer is selected from the group consisting of alginates, rhizobian gum, sodium carboxy methyl cellulose, pullulan, Biosaccharide Gum-1, glucomannane, beta-glucane, pectine, tamarindus indica seed polysaccharide and hyaluronic acid. In an even more preferred embodiment the biopolymer is sodium alginate or hyaluronic acid. In the most preferred embodiment the biopolymer is hyaluronic acid.
In a preferred embodiment the first and/or second composition comprising the at least one biopolymer is an aqueous solution or an emulsion. In a more preferred embodiment both compositions comprising a biopolymer are aqueous solutions or emulsions.
In another embodiment of the present invention the first and/or second composition comprising a biopolymer is a gel or a liquid with low to high viscosity.
The inventors had found in particular, that controlled conditions during the sublimation process and a control of the parameters of the compositions, e.g. salt contents, pH-value, vacuum, used emulsifying agents, allow the control of the average molecular weight of the degraded biopolymer.
In particular the inventors found that combining compositions comprising the same biopolymer with different pH-values leads to a different distribution of the average molecular weight of the biopolymer, which allows differently defined weight distributions depending on the pH-values and/or volume of the compositions.
In one embodiment of the invention the first and/or second frozen composition comprising a biopolymer has a pH-value selected from a range between 1.5 and 8.5. In a preferred embodiment the pH value is selected from a range between 2.5 and 6.
If the first and second composition comprise the same biopolymer it is preferred that the pH value of the composition differs by at least 0.1. In a preferred embodiment the pH-value of first and second composition differs by at least 0.5. In a more preferred embodiment the pH-value of first and second composition differs by at least 1. In the most preferred embodiment the pH value of the first and second frozen composition differs by at least 2.
The inventors had found a direct correlation between the pH-value, the temperature during the sublimation process and the average molecular weight of the biopolymer.
It is evident that the average molecular weight of the final product of the processed biopolymer is directly dependent on the combination of temperature and pH value selected. The inventors further found that it is possible to stack or mix multiple frozen compositions to vary the molecular weight distribution of the final product.
In one embodiment of the invention the maximum temperature during the sublimation process is selected from the range of −40° C. to 150° C. In a preferred embodiment the temperature is selected from the rage of 0 to 140° C. In a more preferred embodiment the temperature is selected from the range of 60 to 130° C. In the most preferred embodiment the temperature is 120° C.
In an alternative embodiment the temperature during the sublimation process is varied during the lyphilization process. In a preferred first embodiment the sublimation is carried out at two temperatures. A schematic overview of preferred temperatures profile is shown in
In one embodiment of the invention the sublimation process is carried out at two different temperatures. Preferably the first temperature is selected from the range of −30° to +40° C. and the second temperature is selected from the range of 60 to 130° C. In a preferred embodiment the first temperature is selected from the range of −20 to 20° C. and the second temperature is selected from the range of 80 to 120° C. In a most preferred embodiment the first temperature is 10° C. and the second temperature is 120° C.
In an alternative embodiment the temperature profile comprises more than two different temperatures. In an alternative embodiment the temperature profile comprises a continuous temperature gradient.
In one embodiment of the invention the pressure during the sublimation step is between 50 μbar and 800 μbar. In a preferred embodiment of the invention the pressure is between 75 μbar and 600 μbar, more preferably between 100 μbar and 400 μbar, even more preferably between 150 μbar and 300 μbar. In a most preferred embodiment the pressure during the sublimation step is 300 μbar.
The biopolymer compositions produced with the process might be purified or isolated from the composition, however it is preferred that no further purification or isolation step is performed. In the most preferred embodiment the biopolymer is directly suitable for further processing and/or use.
The present invention does not only relate to a method for the production of biopolymer compositions with defined average molecular weight, but also to the use of said method for the production of biopolymer compositions with defined average molecular weight and to the biopolymer compositions with defined average molecular weight produced with said method.
In a preferred embodiment the method is used for the productions of biopolymers with defined average molecular weight, which are selected from the group comprising hyaluronic acid, collagen, glucosamino glycans, polysaccharides and fucoidanes. In a more preferred embodiment the biopolymer is a glucosamino glycan. In a more preferred embodiment the method is used for the productions of biopolymers with defined average molecular weight selected from alginates, rhizobian gum, sodium carboxy methyl cellulose, pullulan, Biosaccharide Gum-1, glucomannane, beta-glucane, pectine, tamarindus indica seed polysaccharide and hyaluronic acid. In an even more preferred embodiment the the method is used for the productions of biopolymers with defined average molecular weight selected from sodium alginate or hyaluronic acid. In the most preferred embodiment the method is used for the production hyaluronic acid with a defined average molecular weight.
The inventors found that the present invention is suitable for the production of complex compositions, comprising biopolymers. These compositions comprise at least one biopolymer with defined average molecular weight and other optional components, such as dermatological, pharmaceutical or cosmetic ingredients and just need to be emulsified or dissolved to be used.
The complex compositions may comprise additional biopolymers or other polymers. Any composition is suitable, as long as the composition comprises additionally water.
In a preferred embodiment the first and/or second composition comprises:
In alternative embodiments the first and/or second frozen composition is a gel or a liquid with low to high viscosity.
The first and/or second composition preferably contains further additional cosmetic, dermatological or pharmaceutical ingredients or additions. Non-limiting examples for these ingredients are emollients, cosmetically acceptable ingredients and dyes, perfumes or pharmaceutically active substances like panthenol.
In a preferred embodiment the first and second frozen composition are comprise the same compounds with the exception of the biopolymer or the pH-value. In the most preferred embodiment the first and second frozen composition comprise the same compounds and the same biopolymer and differ only in pH-value.
Non limiting examples for said ingredients or additions are: skin conditioning agents, skin-smoothing agents, agents for skin hydration, e.g. panthenol or panthothenol, natural moisturising factors, such as glycerine, lactid acid or urea. Alternatively a physical or chemical sunscreens, keratolitics, such as α- or β-hydroxy acids, α- or β-ketoacids. Further possible ingredients include radical catchers, anti-ageing agents, vitamins or derivatives thereof, e.g. vitamin C (ascorbic acid) or esters or glycosides thereof, antioxidants, such as catechins or flavonoids.
Further potential ingredients comprise resveratol, gluthation, ferulic acid, Q10, polyphenols, ceramides, saturated and or unsaturated fatty acids and there glycerides. Furthermore esters, such as wax esters, such as jojoba oil, triglycerides in general (neutral oil, argan oil, shea butter) or unsaponifiable components from plant oils.
Further ingredients comprise polysaccharides of vegetable, biotechnological or marine origin, as well as their hydrolysates. Other ingredients might include enzymes, e.g. bromelain, coenzymes, enzyme inhibitors, amino acids, natural and synthetic oligopeptides, peptides such as collagen and elastin, as well as their hydrolysates, neuropeptides, growth factors, alcaloids. In some embodiments the ingredients optionally include phytopharmaca such as aescin, ginsenosides, ruscogenine or aloin. Further polymers are alginates, cellulose derivatives, starch, chitosan, chondroitin sulfate, further synthetic biopolymers with biological function or compatibility
Non-limiting examples of cosmetic additions comprise skin lightening agents, inorganic or synthetic fillers or decorative substances, such as coloring pigments or dyes or particles. Some embodiments of the invention comprise substance for the cosmetic beautification of eyes, lips or face.
In some embodiments the first and/or second frozen composition further comprises therapeutically active agents, such as anti-acne or anti-rosacea agents, antimicrobial agents, such as silver and it's derivatives, iodine or PVP-iodine, antiperspirants, pain relieving substances such as lidocain or ibuprofen, adstringent substances, deodorizing compounds, antiseborrhoeic substances or antiseptics. Furthermore cells or cell components, such as autologous cells, allogenic cells, stem cells or platelet-rich plasma (PRP).
The first and/or second composition preferably contains other ingredients, e.g. stabilizers, preserving agents, to control the final parameters of the product, such a solubility or emulsifiability, mechanical stability, product viscosity or haptics.
In a particular embodiment of the present invention the first and second composition are combined in an appropriate container, which is suitable for the freezing and lyophilization process, as well as optionally able to serve as packaging for the lyophilized composition comprising at least one biopolymer with defined average molecular weight and defined weight distribution.
The invention further relates to the use of said method for the production of compositions comprising a biopolymer with defined average molecular weight and to compositions comprising biopolymers produced according to a method of the present invention.
In one embodiment of the present invention the final composition can serve as a basis for aqueous liquids, emulsions with low viscosity, serum-like liquids, masks, creams, cream masks, patches or segments for topical applications.
Deionized water is transferred to 1 l lab reactor and stirred at 75° C. Hyaluronic acid powder is added and stirred at 75° C. at 700 rpm for 15 min until the material is dissolved. The emulsifier component is added and stirred at 50° C. for 15 min at 1400 rpm under reduced pressure (200 μbar). The oil component is added and stirred at 1400 rpm/45° C./200 μbar for 10 min and subsequently for 5 min at 2100 rpm/45° C./200 μbar. The received emulsion is cooled to room temperature and transferred to 10 ml glass vials and stored overnight at ambient conditions. Samples were frozen in a deep freezer for minimum 16 h and subsequently lyophilized up to maximum target temperature.
As a proof of principle hyaluronic acid was processed according to the invented method. Herein, pure hyaluronic acid, and compositions of hyaluronic acid with MCT neutral oil and Sepinov EMT-10 were lyophilized at varying temperatures.
The following samples were analyzed:
The samples were analyzed using size exclusion chromatography on an HPLC system, using 3 analytical columns. Samples were dissolved in PBS-Buffer with pH 7.4, non-soluble parts were removed by filtration.
The columns were calibrated using dextran/pullulan standards. Molecular masses of the samples were determined based on the said calibration (for the calibration curve see
Only pure hyaluronic acid samples were completely soluble. The soluble components of the Sepinov EMT-10 or neutral oil, do not produce any problematic signals during analysis (see
The results clearly show that the composition and the lyophilization temperature affect the average molecular weight of the hyaluronic acid. While an effect of lyophilization on pure hyaluronic acid at high temperatures occurs and results in a reduced average molecular weight (see
Overall it is clearly visible that the choice of parameters during the lyophilization process is suitable to control the average molecular weight of hyaluronic acid after the lyophilization.
Hyaluronic acid with a molecular weight of 1.478 Mio Da (Contipro, Mw, according to gel permeation chromatography) was dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. The pH was adjusted with hydrochloric acid in the range of 2.9 to 6.21.
7.5 ml HA solution was dispensed in 10 ml glass vials, samples were frozen at −20° C. overnight and placed in a Christ Epsilon 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analyzed by means of gel permeation chromatography against Pullulan and Dextran molecular weight standards.
Independent on adjusted pH, all samples were cleaved showing a maximum of 766 kDa at pH 6.21 and a minimum 84.75 kDa at pH 2.9 (
Four differents types of hyaluronic acid (Contipro/GfN 3010 (MW: 1478 kDa), Principium Cube3 (MW: 733 kDa), Principium Signal-10 (MW: 25 kDa) and Freda mini-HA (MW: 27 kDa)) were dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. Solution were used as is or pH was adjusted to approximately 3.5.
7.5 ml HA solution was dispensed in 10 ml glass vials, samples were frozen at −20° C. overnight and placed in a Christ Epsilon 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. or alternatively the 120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analysed by means of gel permeation chromatography against Pullulan and Dextran molecular weight standards.
High and medium molecular weight hyaluronic acid showed a moderate decay of molecular weight at original pH dissolved in distilled water, whereas molecular weight of substances decayed drastically at low pH, as shown in the following table.
5 g of high molecular weight hyaluronic acid (GfN/Contipro 3010, 1.5 MDa) was dissolved in 465 g of distilled water, heated to 80° C. and stirred by means of a Somakon MP-LB (11) mixing device at 1400 rpm and ambient pressure for 15 minutes.
7.5 g Sepinov EMT-10 (INCI name: Hydroxyethyl acrylate (and) Sodium Acryloyl Dimethyl Taurate Copolymer) was added the pH was adjusted to 3.05 and mixture was stirred at 1400 rpm/200 μbar for further 15 minutes at 80° C.
25 g of medium chain triglyciderides (MCTs) as model oil compound were added and homogenized at 2100 rpm/200 μbar for 5 minutes.
7.5 ml of the resulting emulsion was dispensed in 10 ml glass vials, samples were frozen at −20° C. overnight and placed in a Christ Epsilon 2-10D LSC plus HT device and processed for approximately 20 hours at maximum 40, 60, 80, 100 and 120° C.
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analyzed by means of GPC.
Polymers were dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. The pH of the solutions was measured and the molecular weight distribution of the non-processed polymer solutions were determined by means of size exclusion chromatography against Pullulan and Dextran molecular weight standards diluting the samples to 0.3 wt-% in PBS buffer (pH 7.4).
7.5 ml polymer solution was dispensed in 10 ml glass vials, samples were frozen at −20° C. overnight and placed in a Christ Epsilon 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analysed by means of GPC. The results are shown in the following tables.
Tamarindus
indica Seed
Hyaluronic acid with a molecular weight of 1.478 Mio Da (Contipro, Mw, according to gel permeation chromatography) was dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. One fraction of the solution was used at normal pH, the second fraction was adjusted with hydrochloric acid to pH 2.9.
pH 6.21 HA solution was dispensed in differed volumes from 0.75 to 6.75 ml in 10 ml glass vials. Samples were frozen at −20° C. and stacked with pH 2.9 HA solution at 6.75 to 0.75 ml and frozen again and stored at −20° C. overnight. The corresponding volume ratios are shown in the following table:
Samples were placed in a Christ 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analysed by means of gel permeation chromatography against Pullulan and Dextran molecular weight standards.
Dependant on volume ratio differently shaped molecular weight distributions can be shaped (see
Pullulan with a molecular weight of 371 kDa (Hayashibara, Mw, according to gel permeation chromatography) was dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. One fraction of the solution was used at normal pH (4.9), the second fraction was adjusted with hydrochloric acid to pH 3.5.
pH 4.9 pullulan solution was dispensed in differed volumes from 0.75 to 6.75 ml in 10 ml glass vials. Samples were frozen at −20° C. and stacked with pH 2.9 pullulan solution at 6.75 to 0.75 ml and frozen again and stored at −20° C. overnight. The corresponding volume ratios are shown in the following table.
Samples were placed in a Christ 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analysed by means of gel permeation chromatography against Pullulan and Dextran molecular weight standards.
Dependant on volume ratio differently shaped molecular weight distributions can be achieved (see
Sodium alginate with a molecular weight of 881 kDa (Cargill, Mw, according to gel permeation chromatography) was dissolved in 1 wt-% solution in distilled water at 80° C. for five minutes. One fraction of the solution was used at normal pH (7.15), the second fraction was adjusted with hydrochloric acid to pH 3.5.
pH 7.15 sodium alginate solution was dispensed in differed volumes from 0.75 to 6.75 ml in 10 ml glass vials. Samples were frozen at −20° C. and stacked with pH 3.5 sodium alginate solution at 6.75 to 0.75 ml and frozen again and stored at −20° C. overnight. The corresponding volume ratios are shown in the following table.
Samples were placed in a Christ 2-10D LSC plus HT device and processed for approximately 20 hours according to the 10/120° C. temperature profile shown in
Lyophilised samples were diluted in GPC buffer (pH 7.4) at a concentration of 0.3 wt-% and analysed by means of gel permeation chromatography against Pullulan and Dextran molecular weight standards. Dependent on volume ratio differently shaped molecular weight distributions can be shaped (see
| Number | Date | Country | Kind |
|---|---|---|---|
| 15154173.7 | Feb 2015 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2016/051955 | 1/29/2016 | WO | 00 |
