The present invention relates to a novel method for determining the concentration of a target analyte utilizing an affinity binding assay. In particular, the present invention relates to an assay having improved sensitivity and an expanded dynamic range that can overcome the “hook-effect.”
The quantitative determination of substances by means of affinity binding assays is known. In particular, the determination of antigenic substances by means of immunoassays is known. In conventional immunoassays, detection of an antigen can occur by “sandwiching” the antigen between two antibodies, a detection antibody which is labeled with an optical or calorimetric reporter, and a capture antibody which is typically coupled to a solid support. The measured signal can then be used to determine the concentration of the antigen present in the sample. A sandwich immunoassay is further illustrated in
In conventional immunoassays, multiple wash steps may be employed to permit the detection of an analyte with high sensitivity and dynamic range. In one configuration, for example, an ELISA is performed by immobilizing a capture antibody to a solid surface. A sample containing the target analyte (e.g. a protein) is then added. Analyte that does not complex with the immobilized capture antibody is washed away, while analyte that has been bound to the capture antibody is then detected with the labeled detection antibody.
Homogeneous assays offer the potential for a significantly streamlined work flow. In homogeneous assays, wash steps are omitted, and the target is detected with minimal sample manipulation. One example of a homogeneous assay is shown in
One of the inherent issues with a homogeneous assay is that the absence of wash steps may limit its dynamic range compared to a non-homogeneous assay. As antigen concentration becomes higher, the capture antibodies and detection antibodies each will bind antigen inhibiting the formation of the sandwich complex. This phenomenon manifests itself as a drop in signal, producing a so-called “hook-effect.” The hook-effect is further illustrated in
Referring to
A need exists for a homogeneous assay that can overcome the hook-effect, and has an expanded dynamic range of sensitivity.
The present invention relates to a method for determining the concentration of a target analyte. The method can overcome the hook-effect and expand the dynamic range of accurate detection. The method can be utilized in a homogeneous assay system. The method incorporates binding moieties (e.g., antibodies, oligonucleotides) having different affinity for the analyte to generate different binding profiles. The binding profiles can be analyzed to absolutely determine the concentration of target analyte. In one or more embodiments, the method utilizes a sandwich assay in which a first binding moiety immobilized to a solid support (e.g. a “capture antibody,” or “capture oligonucleotide”), and a second binding moiety labeled with a first signal particle (e.g. a “detection antibody,” or “detection oligonucleotide”), can be incubated with a target analyte (e.g. an antigen, or a nucleic acid). A binding moiety can be a molecule(s) that binds, attaches, or otherwise associates with a specific molecule. The binding, attachment, or association can be chemical or physical. A specific molecule to which a specific binding member binds can be any of a variety of molecules, including, but not limited to, antigens, haptens, proteins, carbohydrates, nucleotide sequences, nucleic acids, amino acids, peptides, enzymes, and the like.
A first sandwich complex, such as illustrated for example in
A third binding moiety immobilized to a solid support, and a fourth binding moiety labeled with a second signal particle, can further be incubated with the target analyte. The third and fourth binding moieties can each specifically bind to the target analyte, and each can have an established affinity for the target analyte. Likewise, a second sandwich complex, such as illustrated in
The method utilizes multiple sandwich complexes, which produce different binding profiles that correlate to the different affinity. The binding moieties can have differing binding affinities for the target analyte, and therefore, incubating the target analyte with two pairs of binding moieties can produce first and second sandwich complexes having different binding profiles. When two binding moiety pairs are used in an assay, two different response profiles can be observed. Target analyte levels can be determined by reading and analyzing the signals generated by the two binding profiles. As an option, more than two binding profiles can be created using two or more sandwich complexes. The first and second signal particles can each generate a detectable signal that is distinguishable, each from the other, and the first and second signals can be detected and measured. A first signal, generated from the first signal particle in the first sandwich complex, and a second signal, generated from the second signal particle in the second sandwich complex, can each be compared to a standard, or a standard reference profile. This is one way to quantify the result. However, it is possible to have only a single standard curve that is based on, e.g., the ratio of the first signal to the second signal. The standard and/or standard reference profile can comprise, for example, the ratio of the first signal to the second signal. Based on the comparison, the concentration of target analyte can be absolutely determined.
The method overcomes the consequences of a hook-effect, such as at high target analyte concentrations. In one or more embodiments, an analyte concentration can be determined by comparing a first signal to a first standard reference profile. Any potential ambiguity resulting from a possible hook-effect can be clarified by comparing a second signal to a second standard reference profile. The method can also provide a more accurate determination of the analyte concentration. Comparing both first and second signals to corresponding standard reference profiles can verify the target analyte concentration.
The present invention further relates to an assay system for detecting a target analyte. The system can overcome the hook-effect and can expand the dynamic range of an assay. The system can be utilized in a homogenous assay system. The system incorporates binding moieties that have different affinity for the target analyte and consequently produce different binding profiles. The binding profile data can be collected and analyzed to determine the concentration of target analyte.
The assay system can utilize a sandwich assay method wherein a sample containing the target analyte is incubated with a pair of binding moieties, one of which is immobilized to a solid support, and the other is labeled with a signal particle. The sample is also incubated with a second pair of likewise immobilized and labeled binding moieties, the second pair having different affinity for the target analyte than the first pair, and labeled with a different signal particle. Each pair of binding moieties can form a sandwich complex with the target analyte, such as illustrated in
The assay system can include an instrument capable of detecting the different signals generated by the signal particles and an analyzer capable of analyzing the detected signals to determine the presence of and/or quantity of target analyte. The system can utilize one or more standard reference profiles prepared from known amounts of target analyte, and the analyzer can compare the detected signals generated from each sandwich complex to the one or more standard reference profiles to determine the quantity of target analyte present.
The accompanying drawings, which are incorporated into and constitute a part of the present invention, illustrate embodiments of the present invention, and together with the general description given above and the detailed description, serve to explain the principles of the present invention.
The drawings and the following detailed description provide information about the present invention including the description of specific embodiments. The detailed description serves to explain the principles of the present invention. The present invention is susceptible to modifications and alternative forms and is not limited to the particular forms disclosed. The present invention covers all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the claims.
The present invention relates to a method for determining the concentration of a target analyte. The term “target analyte,” as used herein, is a substance to be detected in a test sample using the present invention. The analyte can be any substance for which there exists a naturally occurring capture reagent, or for which a capture reagent can be prepared. The target analyte can bind to one or more binding moieties in an assay. The target analyte can include a protein, a peptide, an amino acid, a carbohydrate, a hormone, a nucleic acid, a steroid, a vitamin, a cell, a drug, a bacterium, a virus, and metabolites of, or antibodies to, any of the above substances. The target analyte can comprise, for example, oncology markers, such as prostate specific antigen (PSA), alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and cyclin-dependent kinase inhibitor 2A (p16), human papilloma virus proteins such as E6 and/or E7 proteins, influenza virus, hormones, such as thyroid stimulating hormone (TSH), and human chorionic gonadotropin (hCG), mini-chromosomal maintenance (MCM) family members, cardiac markers, creatine kinase-subtype MB (CK-MB) and troponin.
The term “binding moiety,” as used herein, refers to a molecule that binds, attaches, or otherwise associates with a specific molecule. The binding, attachment, or association can be chemical or physical. A specific molecule to which a specific binding member binds can be any of a variety of molecules, including, but not limited to, antigens, haptens, proteins, carbohydrates, nucleotide sequences, nucleic acids, amino acids, peptides, enzymes, and the like.
The present invention further relates to a method that can expand the dynamic range and the sensitivity of an assay. In particular, the method can expand the dynamic range and sensitivity of a homogeneous assay. As used herein, the term “homogeneous assay” refers to an assay in which no wash steps are performed to remove excess reagent or target. In particular, the present invention pertains to assays in which a target analyte is detected when it forms a sandwich complex with a capture reagent and a detection reagent.
The term “capture reagent,” as used herein, is a molecule or compound capable of binding the target analyte, which can be directly or indirectly attached to a substantially solid support. The term “detection reagent,” as used herein, refers to an agent that is capable of generating a detectable signal, which can be used to assess the presence and/or quantity of the analyte to be detected. The present invention will be described mainly in terms of an immunoassay, wherein the capture reagent and/or detection reagent can comprise, for example, an antibody. The present invention is not, however, limited to antibodies and the capture reagent and/or detection reagent can comprise, for example, a nucleic acid, a nucleic acid binding protein, a receptor, a ligand, a nucleic acid, a complementary nucleic acid, a carbohydrate, a lectin, and the like.
The detection reagent can comprise, for example, a SERS-tag that can produce a detectable Raman signal when illuminated with radiation of the proper wavelength. A SERS-tag can encompass any organic or inorganic atom, molecule, compound or structure known in the art that can be detected by Raman spectroscopy. SERS-tags offer the advantage of producing multiple sharp spectral peaks, allowing a greater number of distinguishable labels to be attached to detection probes.
For purposes of illustration only, the present invention will be described mainly in terms of an immunoassay wherein target analyte 10 comprises an antigen and each binding moiety comprises an antibody. As used herein, the term “antibody,” is used in its broadest sense to include polyclonal or monoclonal antibodies, as well as antigen-binding fragments of such antibodies. An antibody is characterized, for example, by having specific binding activity for an epitope of an analyte. As such, Fab, F(ab′2), Fd, and Fv fragments of an antibody that retains specific binding activity for an epitope of an antigen, are included within the definition of an antibody. An antibody includes, for example, naturally-occurring antibodies as well as non-naturally occurring antibodies, including, for example, single chain chimeric, bifunctional, and humanized antibodies. For explanation purposes only, binding moiety 12 immobilized to solid support 14 may be referred to as a “capture antibody”, and binding moiety 16 labeled with signal particle 18 may be referred to a “detection antibody.” The present invention, however, is not limited to embodiments that utilize antibodies, as shown for example in
Target analyte 20 can comprise nucleic acid, for example, single-stranded DNA or RNA. Binding moiety 22 can comprise nucleic acid, for example, nucleic acid complementary to at least a portion of target analyte 20. Similarly, binding moiety 26 can comprise nucleic acid, for example, nucleic acid complementary to at least a portion of target analyte 20. Binding moiety 22 can be immobilized to solid support 14, and binding moiety 26 can be labeled with signal particle 18. Binding moiety 22 and binding moiety 26 can each comprise nucleic acid and each can have a length ranging from a single nucleotide to about 100 nucleotides, from about 6 nucleotides to about 50 nucleotides, from about 10 nucleotides to about 25 nucleotide, or about 15 nucleotides. Lengths within or outside of these ranges can be used.
Solid support 14 can comprise a support substrate, for example, a membrane or the interior surface of a sample tube or well. In other embodiments, solid support 14 can comprise a solid particle, for example, a microparticle, a nanoparticle, a bead, or the like. In one or more embodiment, solid support 14 can comprise, for example, a magnetic particle. Magnetic particles and magnetic capture assays are further described in, for example, PCT/US08/57700 filed Mar. 20, 2008 to Natan and entitled “Assays Using Surface-Enhanced Raman Spectroscopy (SERS)-Active Particles)”; U.S. Pat. No. 5,945,281 to Prabhu, which was filed on Feb. 2, 1996 and is entitled “Method and apparatus for determining an analyte from a sample fluid”; U.S. Pat. No. 6,514,415 B1 to Natan, which was filed on Oct. 6, 2000 and is entitled “Surface Enhanced Spectroscopy-active Composite Nanoparticles”; and O. Olsvik, “Magnetic Separation Techniques in Diagnostic Microbiology,” Clinical Microbiology Reviews, Vol. 7, 43-54 (1994), the disclosures of which are hereby incorporated herein by reference in their entirety.
The magnetic particles can comprise, for example, paramagnetic, superparamagnetic, or ferromagnetic materials. The magnetic particles can comprise, for example, paramagnetic microspheres approximately 1 micron in diameter, available from Bangs Laboratories, Inc., Fishers, Ind. The magnetic particles are not limited to this size, and can have a diameter ranging, for example, from about 0.05 micron to about 10.0 microns. The magnetic particles can comprise encapsulated magnetic particles, for example, particles comprising a magnetite-rich core encapsulated with a silica or polymer shell. The magnetic particles can comprise, for example, superparamagnetic material, such as 1 micron to 10 micron beads coated with a material, such as a metal oxide, like silica, available from Bioclone, Inc., San Diego, Calif.
Signal particle 18 can comprise any compound capable of generating a measurable electromagnetic signal, for example a fluorescent, luminescent, calorimetric, and/or Raman signal. The signal particle can also comprise a compound capable of generating a radiolabel, although such compounds are not as compatible in methods and assays that require a wash step. The signal particle can be directly labeled with a signal generating compound, or can be indirectly labeled, for example through a linker or bridge compound. In one or more embodiments, signal particle 18 can comprise, for example, a SERS-active particle, also termed “SERS-labeled nanotag,” a “SERS-nanotag,” or “SERS-tag.” A SERS-tag can produce detectable Raman spectra when illuminated with radiation of the proper wavelength. A SERS-tag encompasses any organic or inorganic atom, molecule, compound, or structure known in the art that can be detected by Raman spectroscopy.
SERS-tags offer the advantage of producing sharp spectral peaks, allowing a large number of distinguishable labels. A number of distinct reporter molecules with strong Raman spectra are known and can be used to create distinct “flavors” of SERS-active particles to enable multiplexing capabilities (the term “flavor” indicates particles that provide distinct Raman signatures upon irradiation). A number of different “flavors” can be excited with a single wavelength. SERS-labeled nanotags are further described, for example, in U.S. Pat. No. 6,514,767 B1 to Natan, which was filed on Oct. 6, 2000 and is entitled “Surface Enhanced Spectroscopy-active Composite Nanoparticles; U.S. Pat. No. 7,192,778 B2 to Natan, which was filed on Jan. 16, 2003 and is entitled “Surface Enhanced Spectroscopy-active Composite Nanoparticles”; and U.S. Patent Application Pub. 2005/0158870 A1 to Natan, which was filed on Feb. 4, 2005 and is entitled “Surface Enhanced Spectroscopy-active Composite Nanoparticles”, and PCT/US08/57700 filed Mar. 20, 2008 to Natan and entitled “Assays Using Surface-Enhanced Raman Spectroscopy (SERS)-Active Particles), the disclosures of which are hereby incorporated herein by reference in their entirety. An example of a SERS-active particle is Nanoplex™ Biotags, available from Oxonica Inc, Mountain View, Calif.
Referring again to
In the example shown in
As described above, and as shown in
According to certain embodiments, a method for overcoming the hook-effect and expanding the dynamic range of an assay can utilize binding moieties specific for a target analyte that have different affinity for the analyte. The dynamic range can be expanded beyond the hook-effect by at least about one order of magnitude. This is preferred, but not a requirement. The term “order of magnitude,” as used herein, means a factor of ten (10).
Referring to
Antibodies A2 and A4 can also each specifically bind to the target antigen and each can also have a different affinity for the antigen. A2 can have, for example, a greater affinity for the target antigen than A4. Antibodies A2 and A4 can each be labeled with a signal particle, S1 and S2 respectively (i.e. detection antibody “A2S1” and detection antibody “A4S2”). S1 and S2 can each be capable of generating a detectable signal that is distinguishable from one another. S1 and S2 can each comprise, for example, a SERS-tag, such as Trans-1,2-Bis(4-pyridyl)-ethylene (BPE), or 4,4′-Dipyridyl (DPY). In preferred embodiments S1 and S2 are SERS-tags, which are detected using Surface Enhanced Raman Spectroscopy.
A target antigen (T) can be incubated with a first pair of capture antibody and detection antibody, for example, MA1 and A2S1, and incubated with a second pair of capture antibody and detection antibody, for example, MA3 and A4S2, under appropriate conditions for forming sandwich complexes such as those described above and shown in
Antibodies A1 and A2 can each have a higher affinity for T than antibodies A3 and A4. Accordingly, two different binding profiles can result, as shown in
In one or more embodiments, a standard reference profile for a set of target analyte standards of known concentration can be produced. For the antibody/antigen assay described above, a first standard reference profile can be generated from known amounts of T standard complexed with the same capture antibody and detection antibody pair used to analyze the target antigen. The standard reference profile can resemble, for example, a standard binding curve. The standard binding curve can resemble, for example, the binding profiles shown in
Generally, the concentration of the target analyte can be determined by comparing the first signal and the second signal to the corresponding standard reference profile. The target analyte concentration can be determined, for example, as shown in
Referring to
To resolve this ambiguity, in the present invention, a second sandwich complex comprising, for example, MA3-T-A4S2, can be incorporated in the assay. The second sandwich complex can generate a second measured signal, for example B1. Because S2 can generate a distinguishable signal, signal B1 can be associated with the second sandwich complex. Signal B1 can be compared to a second standard reference profile, and the target antigen concentration can be absolutely determined as X. Alternatively, the second sandwich complex can generate a second measured signal, for example B2. The second measured signal B2 can be again compared to the second standard reference profile and the target antigen concentration can be absolutely determined as Y. The dynamic range of the assay can be expanded beyond target analyte concentrations normally affected by the hook-effect. The dynamic range can be expanded, for example, by at least about one order of magnitude.
In one or more embodiments, a signal intensity ratio, for example a ratio of the first signal and second signal (A/B), can be determined. The signal intensity ratio, A/B, can be compared to a standard reference profile that is based on the ratio of the first signal to the second signal, A/B, for one or more standards of known concentration. In one or more embodiments, for example, the concentration of a target analyte in a sample can be absolutely determined by referencing a signal intensity ratio measured alongside a standard reference profile. As an option, one can use the reference spectrum in the assay instead of a standard curve as mentioned herein.
The present invention does not require two separate binding moiety pairs (e.g., A1-A2, and A3-A4). A single binding moiety pair can be “flipped” wherein each binding moiety can be immobilized or labeled on the opposite particle. The method can utilize a first pair of binding moieties, for example, A1 immobilized to solid support M, and A2 labeled with signal particle S1. A1 and A2 can also be flipped such that A2 can be immobilized to M, and A1 can be labeled with signal particle S2, to provide a second pair of binding moieties. Target analyte T can be incubated with both binding moiety pairs, wherein a first sandwich complex comprising MA1-T-A2S1 and a second sandwich complex comprising MA2-T-A1S2 can be formed. The two “flipped” sandwich complexes can exhibit different binding affinity for T. Accordingly, two different binding profiles can be demonstrated, such as the profiles illustrated in
For example, as shown in
The bar graph in
In an alternative embodiment, binding moiety pairs having different affinity for a target analyte can be generated by altering the immobilization and/or labeling strategy. For example, a first pair is A1 immobilized to a solid support M, and A2 labeled with a signal particle S1. A second pair is A1 immobilized to a solid support M, and A2 labeled with a signal particle S2. A1 can, however, be immobilized to M using one type of chemistry in the first pair, and can be immobilized to M using a different type of chemistry in the second pair. Similarly, A2 can be labeled with S1 using one type of chemistry, and can be labeled with S2 using a different type of chemistry. The different chemistries generate different affinities of A1 and A2 for a target analyte. For example, some chemistries can orient an antibody so that its binding site is more accessible, while other chemistries can make the binding site less accessible, thereby lowering the affinity.
The present invention does not require that the signals for the reagent pair/analyte sandwich complexes be measured separately and/or independently. One or more signals can be measured simultaneously and a combined signal can be provided. Each signal can be generated by a signal particle associated with each sandwich complex, and each signal is capable of being distinguished from one another. Because the signals are distinguishable, the combined signal can be parsed into the separate signals, which can then be analyzed.
For example, a first signal can be generated from a first sandwich complex and a second signal can be generated from a second sandwich complex, wherein each signal is distinguishable from the other. The first signal and the second signal can be measured to produce a combined signal and then the combined signal can be parsed into separate signals. The separate signals can be parsed wherein they are each capable of being compared to a standard reference profile. The first signal and the second signal can be measured simultaneously. An example of this embodiment is explained further in
The present invention is not limited to two binding moiety pairs. In an alternative embodiment, more than two binding moiety pairs can be utilized to determine the concentration of a target analyte. If two binding moiety pairs, for example, do not provide sufficient resolution to unambiguously determine the analyte level in an assay, additional binding pairs can be used. The dynamic range of an assay can be expanded by utilizing more than two binding moiety pairs, wherein each pair can be capable of forming a sandwich complex with the target analyte. Each sandwich complex can have a different affinity for the target analyte, and each sandwich complex can include a signal particle that generates a distinguishable signal. Two or more, such as three to six, or more binding moiety pairs can be utilized.
Referring to
In alternative embodiments, the method and assay can be used for multiplex analysis of a plurality of target analytes. As used herein, the phrase “multiplex” refers to the detection and/or or analysis of more than one target analyte of interest. Multiplex refers to at least 2 different target analytes. In other embodiments at least 3 different target analytes, at least 6 different target analytes, and at least 10 or more different target analytes can be detected and/or analyzed, although the present invention is not limited to the number of target analytes in a multiplex analysis. Multiple sandwich complexes can form with multiple target analytes in the same reaction container.
The method can comprise incubating a plurality of target analytes, for example in a sample, with a plurality of binding moiety pairs under conditions suitable to form sandwich complexes. Each binding moiety pair can comprise a binding moiety labeled with a signal particle that can generate a distinguishable signal. Following sandwich complex formation, the individual signals can be detected in a multiplex manner with a suitable detection device. The individual signals can be analyzed, for example, via a least-squares fitting technique.
Each signal particle can comprise a SERS-tag associated with a unique optical signature. Because each target analyte is bound to a specific detection reagent comprising a known SERS-tag that emits a distinguishable signal, individual signals detected from the sandwich complexes can thus be associated with the identity of the target analyte.
Multiple antibody pairs incorporated together in a no-wash homogeneous assay can lead to the formation of unwanted sandwich complexes. Referring to
Embodiments of the present invention specifically address the problem of unwanted complexes. Two or more binding moiety pairs can utilize the same binding moiety immobilized to a solid support. Two or more binding moiety pairs can utilize the same binding moiety labeled with distinguishable signal particles. Examples of these embodiments are explained further in
Referring to
Similarly, and as shown in
The problem of unwanted complex formation can also be overcome by utilizing a binding moiety that binds to the target analyte to the exclusion of any other binding moiety. For example, a first binding moiety can bind to the target analyte and block or exclude another binding moiety from binding to the target analyte. The first binding moiety can comprise, for example, an antibody, and the other binding moiety can comprise an antibody that binds to the same epitope, or a closely situated epitope, of a target antigen as the first binding moiety.
For example, to illustrate, a pair of capture antibodies, A1 and A3, can be utilized in an assay wherein the antibodies bind to the same epitope on a target antigen. A1 can bind to the target antigen and thus block or exclude further binding by A3. Likewise A3 can bind to the target antigen and block or exclude further binding by A1. In another example, a pair of detection antibodies, A2 and A4, can be similarly used, wherein the antibodies bind to the same epitope. The binding of one detection antibody, for example A2, to the target antigen blocks or excludes binding by the other, in this instance A4, and vice versa. In both examples, the formation of unwanted capture antibody complexes and/or detection antibody complexes can be reduced or eliminated.
The present invention has been described mainly in terms of an immunoassay, but it is not limited in this aspect. Any set of capture/detection binding pairs can be used to detect any analyte of interest. The present invention can be used to detect and quantitate, for example, a nucleic acid. The present invention can be used to detect and quantitate a specific DNA sequence.
The term “nucleic acid” is used broadly herein to mean a sequence of deoxyribonucleotides or ribonucleotides that are linked together by a phosphodiester bond. A nucleic acid can be RNA or can be DNA, which can be a gene or a portion thereof, a cDNA, a synthetic polydeoxyribonucleic acid sequence, or the like, and can be single stranded or double stranded, as well as DNA/RNA hybrid. A nucleic acid can contain nucleoside or nucleotide analogs, or a backbone bond other than a phosphodiester bond.
First binding moiety 52 and second binding moiety 56 can each be capable of binding to target analyte 50. First binding moiety 52 and second binding moiety 56 can each have a nucleotide sequence that is complementary to target analyte 50. First binding moiety 52 and/or second binding moiety 56 can each have a nucleotide sequence that is, for example, 100 percent complementary to target analyte 50, or a range of about 70 percent to about 100 percent complementary, about 80 percent to about 98 percent complementary, or about 90 percent to about 95 percent complementary to target analyte 50. The skilled person is aware of the degree of complementariness and the hybridization conditions required to cause the target analyte to bind to the first (52) and second (56) binding moieties. The nucleotide sequence of first binding moiety 52 and/or second binding moiety 56 can differ from the nucleotide sequence of target analyte 50 by, for example, one nucleotide, although the present invention does not limit the difference to one nucleotide.
As shown in
Target analyte 50 can simultaneously be incubated with the first reagent pair and with a second reagent pair comprising a third binding moiety immobilized to a solid support, and a fourth binding moiety labeled with a signal particle, wherein a first sandwich complex and a second sandwich complex can form (not shown).
The first reagent pair can have a different affinity (i.e., higher affinity or lower affinity) for target analyte 50 than the second reagent pair. For example, the third binding moiety and/or the fourth binding moiety can comprise nucleic acid and have a nucleotide sequence that is different than the nucleotide sequence of first binding moiety 52 and/or second binding moiety 56. The different affinity can result, for example, from one or more mismatches included in the sequence of any or all of the binding moieties. For example, at least one of first binding moiety 52, second binding moiety 56, third binding moiety, and/or fourth binding moiety can differ by only a single nucleotide. The single nucleotide difference, for example, can correspond to the location of a single-nucleotide polymorphism (SNP) represented in target analyte 50.
First sandwich complex 60 can be capable of generating a first signal, and the second sandwich complex can be capable of generating a second signal that is distinguishable from the first signal. The first and second signals can be measured and compared to first and second standard reference profiles, respectively.
The present invention also relates to a system for detecting a target analyte (T), the system having an expanded dynamic range. The system can comprise a sample comprising a target analyte, a first reagent pair capable of forming a first sandwich complex with T, and capable of generating a first signal, a second reagent pair capable of forming a second sandwich complex with T, and capable of generating a second signal, wherein the first reagent pair has an affinity for T that is different than the affinity the second pair has for T, an instrument capable of detecting the first signal and the second signal, and an analyzer capable of analyzing the detected first signal and detected second signal. The system can have an expanded dynamic range that reduces a hook-effect, when compared to an assay system that utilizes solely analyzing the detected first signal. The dynamic range can be expanded by at least about one order of magnitude.
The system of the present invention can comprise an instrument capable of providing an excitation light beam. The excitation light beam can be directed toward the sample, and can be capable of inducing the first reagent pair to generate the first signal and/or inducing the second reagent pair to generate the second signal. The instrument can comprise, for example, a laser. The instrument can be capable of inducing Raman scattering spectra. The instrument can be capable of detecting the Raman scattering spectra. The instrument can comprise, for example, a spectrometer. The instrument can simultaneously detect the first signal and the second signal. Examples of an instrument that can be used in one or more embodiments of the present invention are provided in PCT/US08/57700, the disclosure of which is hereby incorporated herein by reference in its entirety.
The system can be utilized to analyze a sample that comprises a homogenous reaction mixture. The homogenous reaction mixture can comprise, for example, the target analyte, the first reagent pair, and the second reagent pair, added and incubated together to form a first and second sandwich complex, wherein no wash steps were performed before analyzing the sample. The first reagent pair and the second reagent pair can be added together to the sample, and the first and second sandwich complexes can simultaneously form.
The system can further comprise a first standard reference profile, and a second standard reference profile. The analyzer can compare the first signal to the first standard reference profile, and compare the second signal to the second standard reference profile. Based upon the comparison, the analyzer can determine, for example, the target analyte concentration. The analyzer can comprise, for example, an information processing unit or computer so that analytical data can be manipulated or stored electronically.
The system can further comprise a sample tube, wherein the sample, the first reagent pair, and the second reagent pair are added together to the sample tube. The system can further comprise a magnet positioned adjacent to the sample tube, wherein the magnet is capable of attracting the first sandwich complex and the second sandwich complex to form a pellet in the bottom or at the side of the tube. Examples of a sample tube, and examples of a system comprising a magnet that can be utilized in the present invention can be found in, for example, PCT/US08/57700, the disclosure of which is hereby incorporated by reference in its entirety.
The present invention also relates to a composition. The composition can comprise a first sandwich complex comprising a target analyte and a first reagent pair having a first affinity for the target analyte, and capable of generating a first signal, and a second sandwich complex comprising the target analyte and a second reagent pair having a second affinity for the target analyte that is different than the first affinity, and capable of generating a second signal. The first signal and the second signal can be distinguishable from each other. The first signal and/or the second signal can comprise Raman spectra.
The first reagent pair and/or the second reagent pair can comprise a first antibody immobilized to a solid support and a second antibody labeled with a signal particle. The solid support can comprise, for example, a magnetic particle. The signal particle can comprise, for example, a SERS-tag. The first affinity and the second affinity can differ by at least about one order of magnitude.
The target analyte, the first reagent pair, and/or the second reagent pair can comprise nucleic acid. In one or more embodiment, at least one of the target analyte, the first reagent pair, and the second reagent pair can comprise a nucleic acid, and at least one of the target analyte, the first reagent pair, and the second reagent pair can comprise a nucleic acid binding protein.
The target analyte can comprise, for example, protein, carbohydrate, nucleic acid, hormone, drug, metabolite, bacteria, fungus, protozoa, cell, and virus. The target analyte can comprise, for example, oncology markers, such as PSA (prostate specific antigen) AFP (alpha fetaprotein), CEA (carcinoembryonic antigen), and p16, human papilloma virus proteins, such as E6 and/or E7 proteins, influenza virus, hormones, such as TSH, and hCG, mini-chromosomal maintenance (MCM) family members, cardiac markers, creatine kinase-subtype MB (CK-MB) and troponin.
The composition can further comprise at least a third sandwich complex. The third sandwich complex can comprise the target analyte and a third reagent pair having a third affinity for the target analyte that is different than the first affinity and the second affinity, and is capable of generating a third signal. The third signal can be distinguishable from the first signal and the second signal.
The composition can comprise at least a second target analyte. The composition can further comprise at least a third sandwich complex and a fourth sandwich complex. A third sandwich complex can comprise a second target analyte and a third reagent pair having a third affinity for the second target analyte, and can be capable of generating a third signal. A fourth sandwich complex can comprise the second target analyte and a fourth reagent pair having a fourth affinity for the second target analyte that is different than the third affinity, and can be capable of generating a fourth signal. The third signal and the fourth signal can be distinguishable from the first signal, the second signal, and from one another.
Applicants specifically incorporate the entire contents of all cited references in this disclosure. Further, when an amount, concentration, or other value or parameter is given as either a range, preferred range, or a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. Where a range of numerical values is recited herein, unless otherwise stated, the range is intended to include the endpoints thereof, and all integers and fractions within the range. It is not intended that the scope of the invention be limited to the specific values recited when defining a range.
Other embodiments of the present invention will be apparent to those skilled in the art from consideration of the present specification and practice of the present invention disclosed herein. It is intended that the present specification and examples be considered as exemplary only with a true scope and spirit of the invention being indicated by the following claims and equivalents thereof.
This application claims the benefit of the filing date of U.S. Provisional Patent Application No. 61/044,081 filed Apr. 11, 2008, the disclosure of which is hereby incorporated herein by reference.
Number | Date | Country | |
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61044081 | Apr 2008 | US |