Patent Application
20240285735
The present invention relates to methods of wound debridement and stimulation of granulation tissue formation, thereby preparing the wound bed to facilitate and promote the process of wound healing. In particular, the present invention relates to methods of debridement of chronic wounds comprising topically applying to a chronic wound site or bed a composition comprising a proteolytic enzyme mixture obtained from bromelain and a water-soluble gelling agent, the composition being applied to the wound site or bed up to ten times over a period of up to four weeks.
Chronic or hard to heal wounds are a common ailment, afflicting millions of people annually. The majority of chronic wounds are caused by a local or generalized vascular insufficiency that reduces blood flow to the skin and subcutaneous tissue. The most common type of chronic or hard to heal wounds include: pressure ulcers (decubiti or “bed sores”), diabetic ulcers, arterial ulcers, venous ulcers, and post surgical/post trauma ulcers or a combination of these.
Chronic wounds result in a severe damage to the skin. This damage may involve the entire thickness of the skin and may often include deeper tissues. The damaged skin loses the anatomic organization of a healthy skin, the stratum corneum is at least partially destroyed and consequently the inner layers of the skin are no longer protected from the external environment. Moreover, the damaged skin typically contains eschar, diseased and/or abnormal cells that must be removed in order to enable healing. Leaving the eschar in place extends and deepens the damage into the neighboring, undamaged tissues. This eschar also serves as a medium for bacteria growth and a source of infection, contamination and sepsis which may be life threatening.
Studies conducted to investigate the composition and structure of the eschar and necrotic tissue in chronic wounds revealed that it is comprised of multiple protein species representing the extracellular matrix proteins and degradation products of autolytic debridement. These studies indicated that chronic wound debridement is likely to require multiple enzyme specificities to degrade the various constituents of the non-viable and necrotic tissues in chronic wounds.
Removal of the eschar, diseased and/or abnormal cells, also known as “debridement”, is performed by surgical procedures, by mechanical means (dressings changes, bathing), by autolytic procedures (dressings that promote maceration) or by enzymatic means. Surgery is one of the most common procedures of debridement wherein small necrotic areas are excised of the entire damaged skin. This method is limited to small non-tangential surfaces. It is also non selective, involving the removal of large fractions of healthy tissue which, if preserved, could serve as a source for the natural healing processes. Surgical procedures are also more expensive and require medical resources.
Enzymatic debridement is advantageous over mechanical and surgical debridement mainly since it is less painful, more selective and does not require the assistance of well-trained medical personnel. The application of proteolytic enzymes for debridement is well known in the art. These enzymes include those isolated from bacteria and those generally found in plant sources, such as papaya (papain), fig (ficin), and pineapple (bromelain). Hydrolytic enzymes derived from the pineapple plant that are useful for digestion, dissection and separation of non-viable, especially eschar tissue, from viable tissue in a mammalian host are described in U.S. Pat. Nos. 4,197,291; 4,226,854; 4,307,081; 4,329,430 and 5,830,739, among others.
The degree of the therapeutic activity obtained from topical application of proteolytic enzymes is governed, inter alia, by the intrinsic catalytic characteristics of the enzymes. The major problems associated with topical use of compositions comprising proteolytic enzymes are that the catalytic activity of the enzymes is rapidly attenuated due to the typical low pH at the lesion area, adsorption of the enzyme molecules to the surface of the wound bed and/or the surface of the dressing, and inhibition of enzymatic activity by moieties within the wound exudates. An additional challenge with enzymatic debriding products, is the vehicle, inasmuch as an oil-based vehicle can be problematic for proteolytic activity, which requires water for the hydrolysis of the proteins. Therefore, obtaining stable enzymatic formulations is complicated.
Several ointments are currently being marketed for debriding eschar, such as Collagenase Santyl® ointment. These ointments are typically applied daily for several months and used in combination with mechanical/surgical debridement to achieve the desired wound debridement.
U.S. Pat. No. 4,668,228 to Bolton et al., discloses debriding tapes which contain a proteolytic enzyme useful for debridement of eschar and necrotic tissue, e.g., subtilisin, bromelain, in dry powdered form on the adhesive mass surface of an occlusive or semi-occlusive surgical adhesive tape. According to U.S. Pat. No. 4,668,228, when the debriding tape is applied to a burned surface, water from the wound which cannot penetrate the occlusive tape backing activates the debriding enzymes.
U.S. Pat. No. 4,784,653 to Bolton et al., discloses an absorbent adhesive dressing for use in treating wounds of the ulcer and burn type which comprises a three-layer sandwich-type constructions having an occlusive film as the outer layer, an absorbent layer of fibers as the middle layer, and a wet-stick adhesive as the inner wound facing adhesive layer which is made of an acrylic polymer having both hydrophilic and hydrophobic characteristics. According to U.S. Pat. No. 4,784,653, a debriding enzyme may be added to the adhesive mass, if desired.
U.S. Pat. No. 5,271,943 to Bogart et al., discloses therapeutic gels which have a minimum yield point of about 800 poise and a maximum apparent viscosity of about 100,000 cps, which gels comprise water, sodium chloride, and a gelling agent.
U.S. Pat. No. 5,514,370 to Stern et al., discloses pharmaceutical compositions for topical application containing high concentrations of collagenase in non-aqueous excipients. U.S. Pat. No. 5,514,370 further discloses a method of treating a wound which comprises applying thereto a composition consisting essentially of a non-aqueous excipient and collagenase.
U.S. Pat. No. 5,804,213 to Rolf discloses a prepackaged wound dressing which comprises a natural or synthetic hydrocolloid in dry particulate form. According to U.S. Pat. No. 5,804,213, the hydrocolloid in dry particulate form is contained in a compartment of a sealed container separate from moisture. After mixing with water, the admixture is sufficiently fluid to allow it to be poured or spread into a wound. Following application to the wound, the hydrated hydrocolloidal dispersion begins to solidify to form a solid, self-supporting flexible dressing which consists of water, hydrocolloid and a biologically active constituent.
U.S. Pat. No. 6,548,556 to Hobson et al. discloses an enzymatic anhydrous hydrophilic debrider that uses in combination a proteolytic enzyme and an anhydrous hydrophilic Poloxamer carrier.
U.S. Pat. No. 8,062,661 to Caldwell et al. discloses methods of debriding a skin wound which include contacting the skin wound with a hydrogel patch debridement composition and removing the hydrogel patch debridement composition from said skin wound to remove foreign matter from the skin wound.
U.S. Pat. Application Publication No. 2016/0101165 to Salamone et al. discloses a debridement enzyme for necrotic tissue that is not dependent upon proteolytic enzymatic activity but instead utilizes the amylase family of enzymes.
International Application Publication No. WO 2006/054309 assigned to the applicant of the present invention discloses a debriding composition obtained from bromelain useful in debriding eschar tissues and in facilitation of wound healing.
International Application Publication No. WO 2013/011514 assigned to the applicant of the present invention discloses a proteolytic extract obtained from bromelain for the treatment of connective tissue diseases which are associated with excess of collagen deposition, including Dupuytren's disease and Peyronie's disease.
U.S. Pat. No. 11,382,958 by the applicant of the present invention, discloses debriding compositions comprising a proteolytic enzyme mixture obtained from bromelain being in a dry form, and an aqueous gel carrier, wherein, prior to use, the proteolytic enzyme mixture is admixed with the aqueous gel carrier to form a debriding composition useful for debridement and treatment of chronic wounds.
In the healing process of hard to heal wounds, the wound bed needs to be completely clean and well covered with vascularized, healthy granulation tissue in order for the proliferating epidermal cells to migrate and eventually close the wound. Granulation tissue provides new blood vessels and connective tissue that fill the wound bed, promoting the healing process. Its formation is an essential step in the wound healing process and a key factor contributing to the prevention of recurrent chronic wound formation. Thus, the presence of healthy granulation tissue is considered to be a prerequisite for wound closure, and a marker of progress towards wound healing. Furthermore, complete debridement and formation of granulation tissue i.e., wound bed preparation, is a crucial step towards a healing process and potential wound closure. The healing continuum process requires an adequate blood supply for nutrient delivery, gas, and metabolite exchange as dermal and epidermal cells migrate and proliferate within the wound bed. As the majority of hard to heal wounds are characterized by a prolonged or excessive inflammatory phase, formation of healthy granulation tissue is an important clinical outcome.
There is a long-felt and unmet need for improved compositions and methods of enzymatic debridement of chronic wounds which achieve complete wound debridement and promote wound bed preparation to facilitate closure and healing of chronic wounds.
The present invention provides compositions and methods of debridement and granulation tissue formation promotion of wounds and/or treating wounds, particularly of chronic wounds, comprising topically applying to a wound site or bed a debriding formulation in a regimen of up to ten applications during a time period of up to four weeks, wherein the debriding formulation is formulated as a hydrogel comprising: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, and wherein the debriding formulation is maintained in contact with the wound site or bed for at least four hours, thereby achieving debridement of eschar/slough and various forms of devitalized necrotic tissues as well as promoting granulation tissue formation.
Wound debridement is a key process of wound bed preparation (WBP) and is considered an essential intervention in chronic wound management which may promote wound healing and complete wound closure. It is known that enzymatic debridement agents available today for the treatment of chronic wounds, such as Santyl® ointment, are applied daily for long periods of time, e.g., for six or even twelve months, to achieve eschar removal.
It is now disclosed that applying the debriding formulations of the present invention onto chronic wounds up to 10 times, while maintaining the debriding formulation on the wound site for about 24 hours per application, resulted in essentially complete eschar debridement of the chronic wounds. It is further disclosed herein that applying the compositions of the present invention also resulted in promotion of granulation tissue formation, thereby advancing wound bed preparation towards promoting wound healing and complete wound closure. As exemplified hereinbelow, Phase 2 clinical trial results show that the formulations of the present invention not only achieved wound debridement but also promoted substantial granulation tissue formation in chronic wound of patients, significantly more than placebo control arm and other non-surgical standard of care formulations. The compositions and methods of the present invention require a short-term treatment regimen, improve patient compliance, and bring to debridement of chronic or hard to heal wounds within days, i.e., at a faster rate than any enzymatic debridement method known today. Furthermore, the compositions and methods of the present invention unexpectedly promote greater and faster granulation tissue formation. Thus, the methods of the present invention are highly advantageous over known methods of enzymatic wound debridement which are currently being used.
The debriding formulation useful in practicing the methods of the present invention comprises, according to some embodiments, the following constituents:
wherein, prior to use, the composition (a) being admixed with the water (b) to form a debriding formulation characterized by being a homogenous hydrogel having a viscosity in the range of about 2,000,000 cP to about 9,500,000 cP and a pH in the range of about 6.0 to about 8.0, wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w) of the total weight of the debriding formulation.
The debriding formulation of the present invention has in some embodiments a pH ranging from about 6.4 to about 8.0, e.g., a pH of about 7.0. At this pH range, the activity of the proteolytic enzymes is essentially maximal. In order to achieve these pHs, the debriding formulation of the present invention comprises a pH adjusting agent, thus enabling obtaining a highly efficacious enzymatic debriding agent.
It is further disclosed that due to the protein nature of the active agents of the debriding formulation, namely a mixture of proteolytic enzymes obtained from bromelain, this mixture tends to form aggregates or agglomerations. In order to prevent aggregate or agglomerate formation, the debriding formulation further comprises an anti-aggregation or anti-agglomeration agent, thus enabling the formation of a homogenous hydrogel.
It is further disclosed that by virtue of its constituents, the debriding formulation of the present invention is adequately viscous to enable, on one hand, the penetration of the proteolytic enzymes to the eschar tissue of chronic wounds so as to effectively debride the non-viable tissue, and, on the other hand, to be localized to the wound site, with essentially no leakage of the debriding formulation from the wound site.
It is also disclosed that due to its constituents, the preparation process of the debriding formulation is simple, easy, and quick, taking only few minutes, e.g., less than two minutes, to mix the constituents and to obtain the homogenous hydrogel. Due to the case of mixing, the preparation of the debriding formulation of the present invention can be performed by the patient, with no need of assistance by medical personnel. The methods of the present invention are thus particularly advantageous for elderly patients who have chronic or hard to heal wounds.
According to a first aspect, the present invention provides a method for debridement of a wound comprising topically applying to a wound site of a subject in need of such treatment a therapeutically effective amount of a debriding formulation in a regimen of up to ten applications during a time period of up to four weeks, wherein the debriding formulation in the form of a hydrogel comprising: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, and wherein said debriding formulation is maintained in contact with the wound site for at least four hours per application.
According to some embodiments, the wound to be debrided is a chronic wound. According to further embodiments, the chronic wound is selected from the group consisting of a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, a pressure ulcer, a post-operative wound, and a post-trauma wound. Each possibility represents a separate embodiment of the invention. According to further embodiments, the chronic wound is a diabetic lower extremity ulcer or a venous leg ulcer.
According to additional embodiments, applying the debriding formulation is performed in a regimen of up to 10 applications, wherein the debriding formulation is maintained in contact with the wound site for about 4-24 hours, such as for about 6 hours, for about 8 hours, for about 10 hours, for about 12 hours, for about 16 hours, for about 24 hours, or for any integer in between per application per day. Each possibility represents a separate embodiment of the invention. According to a certain embodiment, the debriding formulation is maintained in contact with the wound site for about 24 hours per application for up to 10 applications, alternatively for up to 8 applications. According to an exemplary embodiment, the debriding formulation is applied daily for about 24 hours per application for 10 consecutive days.
According to yet further embodiments, applying the debriding formulation is performed in a regimen of up to 10 applications of every other day, wherein the debriding formulation is maintained in contact with the wound site for about 48 hours per application. According to still further embodiments, applying the debriding formulation is performed in a regimen of up to 8 applications of every other day, wherein the debriding formulation is maintained in contact with the wound site for about 48 hours per application.
According to yet further embodiments, applying the debriding formulation is performed in a regimen of three applications per week for up to 10 applications, or for up to 8 applications, wherein the debriding formulation is maintained in contact with the wound site for a time period selected from the group consisting of about 48 hours per application and about 72 hours per application.
According to still further embodiments, the regimen of applying the debriding formulation as defined in any of the above is repeated once, twice or until the wound is completely debrided. Additionally or alternatively, if eschar reoccurs and wound closure is not yet obtained, the regimen is repeated one, two, or more times until eschar is completely debrided.
According to yet further embodiments, the regimen of applying the debriding formulation is followed by a halt of application of at least one day, such as of two days, three days, four days, five days, six days, one week, two weeks, three weeks, four weeks, or more, or any integer in between. Each possibility represents a separate embodiment of the invention.
According to yet further embodiments, the method further comprises a step of washing the wound site after the at least 4 hours of contact with the debriding formulation, such as, for example, after the about 6 hours of contact, after the about 8 hours of contact, after the about 10 hours of contact, after the about 12 hours of contact, after the about 24 hours of contact, after the about 48 hours of contact, or after the about 72 hours of contact of the debriding formulation with the wound site.
According to still further embodiments, the method can further comprise a step of administering to the subject an active agent selected from the group consisting of anesthetic agents, antibacterial agents, antifungal agents, and anti-inflammatory agents. The active agent, such as, for example, the anesthetic agent can be topically applied to the wound site or can be administered orally or parenterally before application of the debriding formulation, concomitant with the application of the debriding formulation, or after application of the debriding formulation.
According to some embodiments, the debriding formulation to be used in the method of wound debridement of the present invention is a ready to use formulation comprising the following ingredients:
wherein the ready to use debriding formulation, after admixing the composition with water, is a homogenous hydrogel having a viscosity in the range of about 2,000,000 cP to about 9,500,000 cP having a pH ranging from about 6.0 to about 8.0, wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w) of the total weight of the debriding formulation.
According to additional embodiments, the amount of proteins in the debriding formulation ranges from about 2% (w/w) to about 7% (w/w) of the total weight of the debriding formulation, alternatively from about 1% (w/w) to about 5% (w/w) of the total weight of the debriding formulation, further alternatively of about 1% (w/w), 2%, 2.5%, 3%, 4%, 5%, 6%, or of about 7% of the total weight of the debriding formulation. Each possibility represents a separate embodiment of the invention. According to a certain embodiment, the amount of proteins in the debriding formulation is of about 2% (w/w) of the total weight of the debriding composition. According to certain embodiments the amount of proteins in the debriding formulation is about 5%.
According to other embodiment, the water-soluble gelling agent is selected from the group consisting of naturally occurring gelling agents, semi-synthetic gelling agents and synthetic gelling agents. According to further embodiments, the naturally occurring gelling agent is a naturally occurring polysaccharide such as, for example, galactomannans, glucomannans, natural gums, starches, agar, and pectin. Each possibility represents a separate embodiment of the invention. According to a certain embodiment, the water-soluble naturally occurring gelling agent is guar gum present in an amount ranging from about 0.25% (w/w) to about 5% (w/w) of the total weight of the debriding formulation.
According to additional embodiments, the anti-aggregation agent of the debriding formulation is selected from the group consisting of lactose, sucrose, mannitol, trehalose, and glucose. Each possibility represents a separate embodiment of the invention. According to a certain embodiment, the anti-aggregation agent is lactose present in an amount ranging from about 10% (w/w) to about 25% (w/w) of the total weight of the debriding formulation.
According to further embodiments, the pH adjusting agent of the debriding formulation is selected from the group consisting of potassium phosphate, potassium carbonate, sodium phosphate, and sodium carbonate. Each possibility is a separate embodiment of the invention. According to a certain embodiment, the pH adjusting agent is a combination of potassium phosphate dibasic and potassium phosphate monobasic present in an amount ranging from about 2% (w/w) to about 10% (w/w) of the total weight of the debriding formulation.
According to further embodiments, the viscosity of the debriding formulation to be used in the method of the present invention ranges from about 2,000,000 cP to about 7,000,000 cP, alternatively from about 2,400,000 cP to about 6,200,000 cP, alternatively 2,400.00 cP to 8,500,000 cP, or 2,400,000 cP to 9,500,000 cP. Each possibility is a separate embodiment of the invention.
According to another embodiment, the pH of the debriding formulation to be used in the method of the present invention ranges from about 6.0 to about 7.0. According to a certain embodiment, the pH is of about 7.0.
According to some embodiments, water is present in an amount ranging from about 55% (w/w) to about 90% (w/w) of the total weight of the debriding composition.
According to further embodiments, the debriding formulation to be used in the method of the present invention further comprises an agent selected from the group consisting of anti-foaming agents such as, for example, polyethyleneglycols (PEGs), anti-oxidants, and preservatives. According to one exemplary embodiment, the debriding formulation further comprises PEG.
According to yet further embodiments, the debriding formulation further comprises an active agent selected from the group consisting of anesthetic agents, analgesic agents, anti-inflammatory agents, antibiotic agents, anti-fungal agents, growth factors, and agents promoting healing.
According to some embodiments, the wound to be treated by the method of the present invention is a chronic wound and the debriding formulation comprising:
wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w), preferably ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the debriding formulation.
According to further embodiments, the wound to be treated by the method of the present invention is a chronic wound and the debriding formulation comprising:
wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w), preferably ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the debriding formulation.
According to a certain embodiment, the debriding formulation to be used in the method of the present invention comprises:
According to additional embodiments, the debriding formulation to be used in the method of wound debridement of the present invention is prepared by the following steps:
According to another aspect, the present invention provides a method for treating a wound and/or promoting closure of a wound and/or healing a wound comprising a step of topically applying to a wound site of a subject in need of such treatment a therapeutically effective amount of a debriding formulation in a regimen of up to ten applications during a time period of up to four weeks, wherein the debriding formulation present in the form of a hydrogel comprising: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, and wherein said debriding formulation is maintained in contact with the wound site for at least four hours per application as defined in any of the above.
According to another aspect, the present invention provides a debriding formulation comprising:
wherein the composition (a) being admixed with the water (b) to form a debriding formulation characterized by being a homogenous hydrogel having a viscosity in the range of about 2,000,000 cP to about 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, and wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w) of the total weight of the debriding formulation.
According to some embodiments, the debriding formulation comprising:
wherein the composition (a) being admixed with the water (b) to form a debriding formulation characterized by being a homogenous hydrogel having a viscosity in the range of about 2,000,000 cP to about 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, and wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w), preferably ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the debriding formulation.
According to a certain embodiment, the debriding formulation comprises:
According to another aspect, there is provided a debriding formulation for use in the debridement of a wound and/or in treating a wound and/or in promoting closure of a wound and/or in healing a wound, the debriding formulation comprises: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, wherein said debriding formulation being topically applied to a wound site in a regimen of up to ten applications during a time period of up to four weeks, and wherein the debriding formulation being maintained in contact with the wound site for at least four hours per application according to the principles of the present invention.
According to another aspect, the present invention discloses a ready to use chronic wound debriding and granulation tissue promoting composition comprising a homogenous hydrogel having a viscosity in the range of 2,400,000 centipoise (cP) to 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, with the hydrogel containing:
wherein the proteolytic enzymes in the composition are present in an amount ranging from 1% (w/w) to 7% (w/w) of the total weight of the composition.
According to certain embodiments, the debriding and granulation tissue promoting composition does not contain therapeutically effective amounts of amylase enzymes.
According to some embodiments, the anti-aggregation agent of the debriding and granulation tissue promoting formulation is selected from the group consisting of lactose, sucrose, mannitol, trehalose, and glucose and is present in an amount ranging from about 10% (w/w) to about 25% (w/w) of the total weight of the composition.
According to some embodiments, the pH adjusting agent of the debriding and granulation tissue promoting composition is potassium phosphate present in an amount ranging from about 2% (w/w) to about 10% (w/w) of the total weight of the composition.
According to certain embodiments, the debriding and granulation tissue promoting formulation comprises:
According to further embodiments, the proteolytic enzymes in the debriding and granulation tissue promoting formulation are present in an amount of 5% (w/w) and the guar gum is present in an amount of 3.5% (w/w) of the total weight of the debriding and granulation tissue promoting composition.
According to certain embodiments, the chronic wound is selected from the group consisting of a diabetic ulcer, a venous stasis ulcer, a venous leg ulcer, an arterial insufficiency ulcer, a pressure ulcer, and a post-operative wound.
According to further embodiments, the debriding and granulation tissue promoting composition, further comprises one or more of an antifoaming agent, an anesthetic agent, an antibacterial agent, an antifungal agent, an anti-inflammatory agent, an analgesic agent, a growth factor and/or an agent promoting healing.
According to certain embodiments, the ready to use debriding and granulation tissue promoting formulation is sterile.
According to certain embodiments, the present invention provides a method for debridement and promoting granulation tissue formation of a chronic wound comprising topically applying to the chronic wound bed of a subject in need of such treatment a therapeutically effective amount of the ready to use chronic wound debriding and granulation tissue promoting composition; wherein the debriding and granulation tissue promoting composition is topically applied to the chronic wound site in a regimen of up to ten applications during a time period of up to four weeks.
According to another aspect, the present invention provides a method for debridement and promoting granulation tissue formation of a chronic wound comprising topically applying to the chronic wound bed of a subject in need of such treatment a therapeutically effective amount of a debriding and granulation tissue promoting composition comprising a homogenous hydrogel having a viscosity in the range of 2,400,000 centipoise (cP) to 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, with the hydrogel containing:
wherein the proteolytic enzyme mixture in the debriding and granulation tissue promoting composition are present in an amount ranging from 1% (w/w) to 7% (w/w) of the total weight of the composition, and
wherein the composition is topically applied to the chronic wound site in a regimen of up to ten applications during a time period of up to four weeks.
According to some embodiments, the chronic wound is selected from the group consisting of a diabetic ulcer, a venous stasis ulcer, a venous leg ulcer, an arterial insufficiency ulcer, a pressure ulcer, and a post-operative wound.
According to certain embodiments, the debriding and granulation tissue promoting composition is maintained in contact with the wound site for a duration selected from the group consisting of up to 4 hours, about 24 hours and about 48 hours per application.
According to further embodiments, applying the debriding and granulation tissue promoting composition is performed with three applications per week, and wherein the debriding and granulation tissue promoting composition is maintained in contact with the wound site for a duration selected from the group consisting of up to 4 hours, about 24 hours, about 48 hours per application and about 72 hours per application.
According to some embodiments, the regimen of topically applying the composition to the chronic wound is repeated as needed until the wound is completely debrided.
According to some embodiments, the debriding and granulation tissue promoting composition is topically applied to the chronic wound site in a regimen of up to ten applications during a period of one to three weeks.
According to other embodiments, granulation tissue promotion of at least 50 to 60% of the wound is achieved in one to three weeks of treatment. According to further embodiments, granulation tissue promotion of 75% to as much as 95% of the wound is achieved in less than 2 weeks of treatment. According to further embodiments, granulation tissue promotion of 100% of the wound may be achieved in less than 2 to 3 weeks.
According to certain embodiments, the proteolytic enzyme mixture is present in an amount of 5% (w/w); the guar gum is present in an amount of 3.5% (w/w) of the total weight of the debriding and granulation tissue promoting composition; the anti-aggregation agent is selected from the group consisting of lactose, sucrose, mannitol, trehalose, and glucose and is present in an amount ranging from 10% (w/w) to 25% (w/w) of the total weight of the debriding and granulation tissue promoting composition; and wherein the pH adjusting agent is potassium phosphate present in an amount ranging from 2% (w/w) to 10% (w/w) of the total weight of the debriding and granulation tissue promoting composition.
According to another aspect, the present invention discloses a method for debridement and promoting granulation tissue formation of a chronic wound comprising topically applying to the chronic wound site of a subject in need of such treatment a therapeutically effective amount of a ready to use chronic wound debriding and granulation tissue promoting composition consisting of a homogenous hydrogel having a viscosity in the range of 2,400,000 centipoise (cP) to 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, the hydrogel containing:
wherein the composition optionally contains one of more of an antifoaming agent, an anesthetic agent, an antibacterial agent, an antifungal agent, an anti-inflammatory agent, an analgesic agent, a growth factor and/or an agent promoting healing;
wherein proteolytic enzyme mixture in the composition are present in an amount ranging from 1% (w/w) to 7% (w/w) of the total weight of said composition; and
wherein the composition does not contain therapeutically effective amounts of amylase enzymes.
These and other embodiments of the present invention will be better understood in relation to the figures, description, examples and claims that follow.
The present invention provides compositions and methods for debridement of a wound and/or for treating a wound and/or for promoting closure of a wound and/or for healing a wound comprising a step of topically applying to a wound site of a subject in need of such treatment a therapeutically effective amount of a debriding formulation in a regimen of up to ten applications during a time period of up to four weeks, wherein the debriding formulation present in the form of a hydrogel comprising: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, and wherein said debriding formulation is maintained in contact with the wound site for at least four hours per application. The present invention further provides a debriding formulation in the form of a hydrogel which comprises a proteolytic enzyme mixture obtained from bromelain and a water-soluble gelling agent other than a cross-linked polymer of acrylic acid.
It is now disclosed that applying the debriding formulation of the present invention on a chronic wound induced in pigs for up to 10 applications, when the debriding formulation is maintained in contact with the wound site for 24 hours per application, resulted in essentially complete debridement of the chronic wounds. Similar debridement can be achieved if the debriding formulation is applied to a chronic wound three times a week for up to 10 applications when the debriding composition is maintained in contact with the wound site twice for 48 hours per application and once for 72 hours per application.
As exemplified hereinbelow, Phase 2 clinical trial results show that the formulations of the present invention not only achieved wound debridement but also promoted substantial granulation tissue formation in chronic wound of patients.
The present invention provides a debriding and granulation tissue promoting composition comprising a proteolytic enzyme mixture obtained from bromelain as an active ingredient and various excipients.
The term “proteolytic enzyme mixture obtained from bromelain” as used throughout the specification and claims refers to an enzymatic preparation partially purified from bromelain.
The term “bromelain” refers to a protein extract derived from the stems of pineapple plants. Certain extracts of this type can be purchased commercially.
The proteolytic enzyme mixture obtained from bromelain (also termed Debrase® or NexoBrid®) and the preparation thereof are disclosed in WO 2006/054309 and WO 2013/011514, the content of which is incorporated by reference as if fully set forth herein. The proteolytic enzyme mixture obtained from bromelain comprises at least two of the cysteine proteases present in bromelain: stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31). The proteolytic mixture can further comprise one or more of the cysteine protease precursors of bromelain such as, for example, ananain (EC 3.4.22.31) precursor, fruit bromelain (EC 3.4.22.33) precursor, and stem bromelain (EC 3.4.22.31) precursor. The proteolytic mixture can further comprise cysteine protease fragments (see, for example, WO 2006/054309), a jacalin-like lectin, and/or bromelain inhibitors. According to a certain embodiment, the proteolytic mixture obtained from bromelain comprises stem bromelain (EC 3.4.22.32), ananain (EC 3.4.22.31), a cysteine protease precursor of bromelain, and a jacalin-like lectin.
The proteolytic enzyme mixture can be obtained by the procedure disclosed in WO 2013/011514. As the last step of the preparation, the proteolytic mixture is lyophilized and stored as a lyophilized powder until use.
The proteolytic enzyme mixture is highly stable and can be stored at 2-8° C. for long periods of time, e.g., up to three years. After this period of time, the proteolytic enzyme mixture maintains at least 90% of the original debriding activity which is determined immediately after the preparation process.
The proteolytic enzyme mixture is denoted throughout the specification and claims as the active principal ingredient (API). According to the invention, the amount of proteins, or alternatively the amount of API, in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w) of the total weight of the debriding formulation. According to additional embodiments, the amount of proteins or API ranges from about 1% (w/w) to about 7% (w/w), such as of about 1% (w/w), 2%, 3%, 4%, 5%, 6%, 7% of the total weight of the debriding formulation, or alternatively of about 2% to 5% (w/w) of the total weight of the debriding formulation.
The terms “dry”, “dried”, “lyophilized” or “powdered” composition as used interchangeably throughout the specification and claims refer to the composition which contains water in an amount of no more than about 5% (w/w) of the total weight of the composition, alternatively water is present in an amount of no more than about 3%, 2%, 1%, 0.5%, or further alternatively no more than about 0.1% (w/w) of the total weight of the composition. According to a certain embodiment, the composition is devoid of water.
The term “hydrogel” as used herein refers to an aqueous composition capable of maintaining a gel-like form.
The term “homogenous” hydrogel means a hydrogel having uniform viscosity (e.g., well mixed throughout).
The excipients of the debriding formulation are all pharmaceutically acceptable. The term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U. S. Pharmacopeia or other generally recognized pharmacopeia for use in humans.
The term “about” refers to a value which is 10% above or below the indicated value.
According to some embodiments, the excipients of the debriding formulation are water-soluble. The term “water soluble” refers to an agent which typically has solubility in water in the range of 1 gr/ml to 1 gr/30 ml at room temperature.
The water-soluble gelling agent can be a naturally occurring gelling agent, a semi-synthetic gelling agent, and a synthetic gelling agent. The gelling agents according to the present invention do not include cross-linked polymers of acrylic acid.
The water-soluble naturally occurring gelling agent include, but are not limited to, water-soluble naturally occurring polysaccharides such as, for example, galactomannans, glucomannans, starches, agar, pectins, alginates, carrageenans, or a combination thereof. Each possibility represents a separate embodiment. Non-limiting examples of galactomannans and glucomannans are guar gum, locust bean gum, xanthan gum, gum acacia, gum tragacanth, gellan gums, and mixtures thereof. Each possibility represents a separate embodiment. According to a certain embodiment, the water-soluble naturally occurring gelling agent is guar gum.
Other biopolymers include, for example chitin, chitosan, collagens, gelatin, glycosaminoglycans such as, for example, heparin, chondroitin sulfate, dermatan sulfate, and heparan sulfate, proteoglycans, fibronectins, and laminins.
Semi-synthetic gelling agents include, but are not limited to, cellulose ethers (e.g. hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, hydroxy propylmethyl cellulose), polyvinylpyrrolidone, polyvinylalcohol, hydroxypropyl guar gum, and the like.
The synthetic gelling agents include, but are not limited to, carboxyvinyl polymers, polyvinylpyrrolidone, polyvinyl acetate polymers, polyvinyl chloride polymers, polyvinylidene chloride polymers and the like.
The debriding composition can further comprise at least one excipient selected from the group consisting of an anti-aggregation agent and a pH adjusting agent.
The anti-aggregation or anti-agglomeration agent suitable for practicing the present invention is any known anti-aggregation agent, including but not limited to a water-soluble saccharide or oligosaccharide, for example, lactose, sucrose, mannitol, trehalose, sorbitol, and glucose. Each possibility represents a separate embodiment. According to a certain embodiment, the anti-aggregation agent is lactose.
The pH adjusting agent preferably has a pKa of above 6.0. In some embodiments, the pH adjusting agent can be any known pH adjusting agent such as, for example, potassium phosphate, potassium carbonate, sodium carbonate, and sodium phosphate. According to some embodiments, the pH adjusting agent is a combination of potassium phosphate monobasic and potassium phosphate dibasic present in an amount ranging from about 2% (w/w) to about 10% (w/w) of the total weight of the debriding formulation. It is now disclosed that higher amounts of potassium phosphate monobasic and potassium phosphate dibasic in the debriding formulation cause bleeding at the application site. It is therefore disclosed that if the pH adjusting agent is a combination of potassium phosphate monobasic and potassium phosphate dibasic, their total amount is preferably not higher than about 10% of the total weight of the debriding formulation in order to achieve efficient debridement without undesirable bleeding.
The composition can further comprise an anti-foaming agent. Anti-foaming agents are known in the art and include, but not limited to, polyethylene glycols, e.g., PEG-1450, PEG-3350, and the like. The composition can further comprise a preservative such as, for example, benzyl alcohol, parabens, methyl—or propylhydroxybenzoates; and/or an anti-oxidant such as, for example, ascorbic acid, dihydroquinone, butylated hydroxytoluene and dithiothreitol.
The composition can further comprise an anesthetic agent, an antibacterial agent, an antifungal agent, an anti-inflammatory agent, an analgesic agent, a growth factor and/or an agent promoting healing.
The anesthetic agents include, but are not limited to, amethocaine (tetracaine), lignocaine (lidocaine), xylocaine, bupivacaine, prilocaine, ropivacaine, benzocaine, mepivocaine, cocaine. Each possibility represents a separate embodiment.
The antibacterial agents include, but are not limited to, amanfadine hydrochloride, amanfadine sulfate, amikacin, amikacin sulfate, amoglycosides, amoxicillin, ampicillin, amsamycins, bacitracin, beta-lactams, candicidin, capreomycin, carbenicillin, cephalexin, cephaloridine, cephalothin, cefazolin, cephapirin, cephradine, cephaloglycin, chilomphenicols, chlorhexidine, chloshexidine gluconate, chlorhexidine hydrochloride, chloroxine, chlorquiraldol, chlortetracycline, chlortetracycline hydrochloride, ciprofloxacin, circulin, clindamycin, clindamycin hydrochloride, clotrimazole, cloxacillin, demeclocycline, diclosxacillin, diiodohydroxyquin, doxycycline, cthambutol, ethambutol hydrochloride, erythromycin, crythromycin estolate, erythromycin stearate, farnesol, floxacillin, gentamicin, gentamicin sulfate, gramicidin, griseofulvin, haloprogin, haloquinol, hexachlorophene, iminocylcline, iodochlorhydroxyquin, kanamycin, kanamycin sulfate, lincomycin, lincomycin, lincomycin hydrochloride, macrolides, meclocycline, methacycline, methacycline hydrochloride, methenine, methenamine hippurate, methenamine mandelate, methicillin, metronidazole, miconazole, miconazole hydrochloride, minocycline, minocycline hydrochloride, mupirocin, nafcillin, neomycin, neomycin sulfate, netilmicin, netilmicin sulfate, nitrofurazone, norfloxacin, nystatin, octopirox, olcandomycin, or cephalosporins, oxacillin, oxytetracycline, oxytetracycline hydrochloride, parachlorometaxylenol, paromomycin, paromomycin sulfate, penicillins, penicillin G, penicillin V, pentamidine, pentamidine hydrochloride, phencthicillin, polymyxins, quinolones, streptomycin sulfate, tetracycline, tobramycin, tolnaftate, triclosan, trifampin, rifamycin, rolitetracycline, silver salts, spectinomycin, spiramycin, streptomycin, sulfonamide, tetracyclines, tetracycline, tobramycin, tobramycin sulfate, triclocarban, triclosan, trimethoprim-sulfamethoxazole, tylosin, vancomycin, and tyrothricin. Each possibility represents a separate embodiment.
The antifungal agents include, but are not limited to, nystatin, clotrimazole, miconazole, ketoconazole, fluconazole, thiabendazole, cconazole, clotrimazole, isoconazole, tiabendazole, tioconazole, sulconazole, bifonazole, oxiconazole, fenticonazole, omoconazole, sertaconazole, and flutrimazole. Each possibility represents a separate embodiment.
The anti-inflammatory agent can be non-steroidal, steroidal, or a combination thereof. Non limiting examples of non-steroidal anti-inflammatory agents include oxicams, such as piroxicam, isoxicam, tenoxicam, sudoxicam; salicylates, such as aspirin, disalcid, benorylate, trilisate, safapryn, solprin, diflunisal, and fendosal; acetic acid derivatives, such as diclofenac, fenclofenac, indomethacin, sulindac, tolmetin, isoxepac, furofenac, tiopinac, zidometacin, acematacin, fentiazac, zomepirac, clindanac, oxepinac, felbinac, and ketorolac; fenamates, such as mefenamic, meclofenamic, flufenamic, niflumic, and tolfenamic acids; propionic acid derivatives, such as ibuprofen, naproxen, benoxaprofen, flurbiprofen, ketoprofen, fenoprofen, fenbufen, indopropfen, pirprofen, carprofen, oxaprozin, pranoprofen, miroprofen, tioxaprofen, suprofen, alminoprofen, and tiaprofenic; pyrazoles, such as phenylbutazone, oxyphenbutazone, feprazone, azapropazone, and trimethazone. Extracts of these non-steroidal anti-inflammatory agents may also be employed. Each possibility represents a separate embodiment.
Non-limiting examples of steroidal anti-inflammatory drugs include corticosteroids such as hydrocortisone, hydroxyl-triamcinolone, alpha-methyl dexamethasone, dexamethasone-phosphate, beclomethasone dipropionates, clobetasol valerate, desonide, desoxymethasone, desoxycorticosterone acetate, dexamethasone, dichlorisone, diflorasone diacetate, diflucortolone valerate, fluadrenolone, fluclorolone acetonide, fludrocortisone, flumethasone pivalate, fluosinolone acetonide, fluocinonide, flucortine butylesters, fluocortolone, fluprednidene (fluprednylidene) acetate, flurandrenolone, halcinonide, hydrocortisone acetate, hydrocortisone butyrate, methylprednisolone, triamcinolone acetonide, cortisone, cortodoxone, flucetonide, fludrocorisone, difluorosone diacetate, fluradrenolone, fludrocortisone, diflurosone diacetate, fluradrenolone acetonide, medrysone, amcinafel, amcinafide, betamethasone and the balance of its esters, chloroprednisone, chlorprednisone acetate, clocortelone, clescinolone, dichlorisone, diflurprednate, flucloronide, flunisolide, fluoromethalone, fluperolone, fluprednisolone, hydrocortisone valerate, hydrocortisone cyclopentylpropionate, hydrocortamate, meprednisone, paramethasone, prednisolone, prednisone, beclomethasone dipropionate, and triamcinolone. Each possibility represents a separate embodiment.
Analgesic agents include, but are not limited to, codeine, hydrocodone, oxycodone, fentanyl, and propoxyphene. Each possibility represents a separate embodiment.
The growth factors include, but are not limited to, epidermal growth factors, fibroblast growth factors, insulin-like growth factors, and the like.
Agents promoting healing include, but are not limited to, hyaluronic acid and the like.
The viscosity of the gel formulations of the present invention can be measured by any known means. According to some embodiments, an absolute viscometer with plate geometry can be used to calculate the viscosity of the gel formulations described herein. The viscosity ranges referred to herein are all measured at room temperature.
According to the principles of the present invention, the composition (a) which is present in a dry or powdered form and the water (b) can be placed in a first compartment and a second compartment, respectively, of a single container or can be placed in two separate containers. Before use, the composition (a) and the water (b) are admixed to form the debriding formulation.
The debriding and granulation tissue promotion formulations of the present invention are of low bacterial bioburden, and therefore the formulations of the present invention reduce the risk of further contaminating the wound site. According to some embodiments, the debriding formulations are sterile.
According to some embodiments, the debriding formulation comprises:
wherein the composition (a) being admixed with the water (b) to form a debriding formulation characterized by being a homogenous hydrogel having a viscosity in the range of about 2,000,000 cP to about 8,500,000 cP and a pH ranging from about 6.0 to about 8.0, and wherein the amount of proteins in the debriding formulation ranges from about 0.5% (w/w) to about 7% (w/w) of the total weight of the debriding formulation.
According to some embodiments, the debriding formulation comprising:
It is to be understood that the debriding formulations of the present invention are formulated as gels, i.e., hydrogels, and as such are applied on to the wound site. Preferably, the debriding formulations are not patch formulations. According to some embodiments, the formulations are devoid of adhesive agents, and thus the formulations are non adhesive.
The present invention provides a method for debridement and promoting tissue granulation formation of a skin wound and/or treating a skin wound comprising a step of topically applying to a wound site of a subject in need of such treatment a therapeutically effective amount of a debriding formulation in a regimen of up to ten applications during a time period of up to four weeks, wherein the debriding formulation present in the form of a hydrogel comprising: (i) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32) and ananain (EC 3.4.22.31); and (ii) a water-soluble gelling agent, wherein the water-soluble gelling agent is other than a cross-linked polymer of acrylic acid, and wherein said debriding formulation is maintained in contact with the wound site for at least four hours per application.
Conventional debridement practices such as protecting the peri-wound can also be included in the methods of the present invention.
According to some embodiments, the wound is a chronic or hard to heal wound.
The terms “chronic wound”, “chronic skin wound” or a “hard to heal wound” as used interchangeably throughout the specification and claims refer to a wound that has failed to proceed through an orderly and timely series of events to produce a durable structural, functional, and/or cosmetic closure as wounds do. Wounds that do not heal within one month are considered chronic wounds.
According to some embodiments, the chronic wound is selected from the group consisting of a diabetic ulcer, a venous stasis ulcer, an arterial insufficiency ulcer, a pressure ulcer, a post-operative and a post trauma wound. Each possibility represents a separate embodiment. According to further embodiments, the chronic wound is a diabetic lower extremity ulcer or a venous leg ulcer.
The term “debridement of a wound” as used herein refers to the removal of nonviable tissue: necrotic eschar, slough or fibrin, foreign material, and bacteria/biofilm from a wound site or wound bed. Necrotic eschar is a thin or thick, leathery, devitalized, black, brown or tan tissue, whereas slough and biofilm are exudative, white or yellow-greenish mottled, tenuous tissue on the wound bed. Necrotic tissue, foreign material and bacteria impede the body's attempt to heal by producing or stimulating the production of metalloproteases that interfere with the local wound-healing process. This hostile environment allows bacteria to proliferate, further colonize the wound within the exudates, debris, and purulent discharges (“slough”) that cover the wound bed. In addition, the bacteria secrete structural products that together with the slough form the biofilm, thus protect their colonies from potential destruction. The bacteria produce their own wound-inhibiting enzymes and, more significantly, consume much of the scarce, available local resources that are necessary for wound healing.
According to some embodiments, debridement of a wound refers to removal of at least 50%, alternatively of at least 75% of the non-viable tissue which is present prior to treatment. Each possibility represents a separate embodiment of the invention. According to certain embodiments, debridement of a wound refers to removal of at least 90%, or of at least 95%, and preferably of 100% of the non-viable tissue which is present prior to treatment; such debridement, namely of 90% or more of the non-viable tissue present prior to treatment is referred throughout the specification and claims as “complete debridement of a wound”.
In chronic or hard to heal wounds several different factors may play an important role. Exposed surfaces such as bone, tendons, fascia or even fat do not support cellular proliferation and they dry and become foreign bodies such as synthetic implants. Any interference with local blood supply (arterial, venous, lymphatic, pressure etc.) may cause a wound to become hard to heal and chronic. Granulation tissue may become recalcitrant, atrophic, lose its rich vascular matrix, become darker and opaque in color and will not take any part in the wound healing and closure processes.
The term “wound bed preparation” (or “WBP”) as used herein refers to a wound bed that is completely debrided and covered with granulation tissue which results from a proper treatment application of the formulation in order to accelerate endogenous healing or to facilitate the effectiveness of other therapeutic measures. It is a process of debriding, removing various “burdens” within both the wound and the patient that impede healing. Burdens within the wound include exudate, bacteria, biofilm and necrotic/cellular debris. The overall health status of the patient is important to the healing process. In chronic or hard to heal wounds complete removal of the offending eschar, slough or biofilm may result in a clean wound bed, yet such a wound bed may still be inadequate for future healing if the patient's systemic or the extremity's condition cannot support it.
A wound bed prepared for healing is one without eschar, slough, fibrin or biofilm that also has a viable bed of healthy tissues and/or healthy granulation tissue (level>7 in the granulometer scale or clinical assessment of >75% of granulation tissue coverage of the wound bed) that will allow the wound to close spontaneously by scarring and contracture-epithelialization (optionally using modalities such as biological dressings, wound-healing enhancing dressings, synthetic wound dressings, vacuum or ozone wound healing systems) over the viable, clean bed or will support autologous STSG (Split Thickness Skin Graft) or skin allografting.
Granulometer scale refers to a scale where the granulation is graded according to the color of the wound: 1-Black, 2-Yellow-green, 3-Yellow-green with red spots, 4-Yellow-green with red patches covering approximately 50% of the wound area, 5-Red with yellow patches covering approximately 30% of the wound area, 6-Red with few small yellow spots, 7-Light red: 8-Vivid red.
The term “wound closure” refers to the process of regenerating the covering cell layers of a tissue. Thus, promoting wound closure means creating a positive effect in the regeneration of the covering cell layers. The positive effect can be an acceleration of the regeneration process or a decrease of the damaged area of the wound. Wound closure is also defined as full epithelialization without drainage, and without need for additional dressing, confirmed at two consecutive study visits 2 weeks apart.
The terms “composition” and “formulation” are used interchangeably to describe the preferred hydrogel of the invention that is utilized for the treatment of chronic wounds.
The term “therapeutically effective amount” is that amount of the proteolytic enzyme mixture which is sufficient to provide a beneficial effect to the subject to which the composition is administered.
According to some embodiments, the debriding formulation can be applied to a wound site up to 10 applications, wherein the debriding formulation is maintained in contact with the wound site for at least four hours per application per day.
According to additional embodiments, the debriding formulation can be applied in a regimen of up to 10 times to a wound site, wherein the debriding formulation is maintained in contact with the wound site for about 24 hours per application. Thus, the debriding formulation can be applied daily for up to 10 consecutive days so as to be maintained in contact with the wound site for about 24 hours per application or can be applied continuously 1, 2, 3, 4, 5, 6, 7, 8, or 9 applications for about 24 hours per application with a halt of application in between of one day or more as required.
According to additional embodiments, the debriding formulation can be applied in a regimen of up to 10 times to a wound site, wherein the debriding formulation is maintained in contact with the wound site for about 48 hours per application. Thus, the debriding formulation can be applied every other day for up to 20 consecutive days or can be applied 1, 2, 3, 4, 5, 6, 7, 8, or 9 times every other day with a halt of application in between of one day or more as required.
According to further embodiments, the debriding formulation can be applied in a regimen of up to 10 times to a wound site, three times a week, wherein the debriding formulation is maintained in contact with the wound site for a duration selected from the group consisting of 48 hours per application and 72 hours per application.
According to additional embodiments, the debriding formulation can be applied in a regimen of up to 10 times to a wound site, wherein the debriding formulation is maintained in contact with the wound site for about 72 hours per application.
According to certain embodiments, the debriding formulation is maintained in contact with the wound site up to about 72 hours per application.
After the contact of the debriding formulation with the wound site for the indicated application time, such as after at least 4 hours treatment, or after 24 hours treatment, or after 48 hours treatment, or after 72 hours treatment, the wound site can be washed. Thus, the methods of the present invention can further comprise a step of washing the wound site after said contact, prior to a subsequent application of the debriding formulation. If a halt of application is performed, the wound site can be covered with a moist dressing such as moist saline gauze.
According to some embodiments, the methods of the present invention can further comprise a step of covering the debriding formulation with an occlusive layer or dressing to maintain or hold the composition at the wound site.
According to additional embodiments, the method of the present invention can further comprise a step of protecting the wound edges and the peri-wound skin during debridement.
The ranges of numerical values indicated throughout the specification and claims include any integer in between.
It is to be understood that the regimens defined in any of the above can be repeated one, two, three or more times until the eschar/necrotic tissue is completely debrided, optionally with a halt of application. The halt of application can be of days, weeks or months. The regimen of application of the formulation can be repeated as necessary to debride eschar. If eschar reoccurs, the regimen of application of the formulation can be repeated as necessary to debride eschar.
The present invention encompasses combination therapy wherein the methods of the present invention can be combined with known debridement methods, such as, surgical or sharp debridement. According to some embodiments, the methods of the present invention can be performed prior to surgical or sharp debridement. Alternatively, the methods of the present invention can be performed after surgical or sharp debridement.
According to some embodiments, the amount of API applied ranged between about 0.1 gr to about 2 gr of the sterile lyophilized proteolytic enzyme mixture per 100 cm2 of wound surface. According to additional embodiments, the amount of hydrogel applied to a wound site is of about 20 gr per 100 cm2 of wound surface.
The debriding formulations were prepared by admixing the dried or powdered composition which contained API, guar gum, lactose, potassium phosphate dibasic and monobasic, and PEG-3350, with water to form the hydrogel having a homogenous appearance and which has a viscosity ranging from 2,400,000 cP to 6,200,000 cP.
The aim of this study was to determine the dose of the active ingredients in the gel formulation which provides maximal efficacy of eschar debridement of chronic wounds.
A chronic wound model was established in crossbred domestic pigs.
Prior to application of the gel formulation, wound edges were protected with thick layer of Vaseline. Each wound site received˜2 g of the gel formulation to cover the wound for 24 hours, and bandaged with non absorbing dressing. Each wound was photographed before and after each application. The following doses were examined: placebo (0%), 0.1%, 0.5%, 1%, 2%, and 5%.
This procedure was followed for up to 11 consecutive daily treatments or until clean wound bed was achieved. This period was denoted the “Treatment period”. The treatment period was followed by two weeks “recovery period” with no treatments. In the recovery period the wounds were photographed 3 times a week.
The wound area, clean area and eschar volume were evaluated visually, measured by ImageJ software (NIH, MD, USA) and analyzed by JMP statistical software (SAS Inc., NC, USA).
On the first treatment day, all wounds were covered by a full eschar. The eschar composed of 2 distinct areas:
At the beginning of the treatment period, the chronic wounds already developed eschar.
The clean area was calculated as percent from total wound size. The volume of the eschar was calculated as percentage from the eschar volume just before the first treatment, taking into account both the area and the thickness of eschar.
The efficacy of the treatments was evaluated by measuring the area under the curve (AUC) where the x axis is the day of treatment and y axis is the percent clean area or percent eschar volume. The more effective the treatment, the larger is the area under the curve for percent clean area and the smaller the area under the curve for eschar volume.
To assess the irritation caused by the formulation, five blinded assessors scored each wound according to the photographs of the entire experiment. Debridement results are shown in Table 1.
The percent of clean area at the end of treatments out of the initial wound area was found to be significantly dependent on the amount of API. The dependency on the amount of API was linear (
At a dose of 5% of API in the gel formulation, an average of 82% of the wound was clean.
The percent of eschar at the end of treatments out of the initial amount was found to be significantly dependent on the amount of API. The dependency on the amount of API was linear.
At a dose of 5% of API in the gel formulation, an average of 93% of the eschar was removed.
The Area Under the Curve (AUC) where the x Axis is Day of Treatment and the y Axis is the Percent Clean Area:
This parameter shows the cleaning efficacy of the treatments: the more effective the treatments are, the larger the AUC. The AUC of percent clean area during treatments was significantly dependent on the amount of API. As shown in
The Area Under the Curve (AUC) where the x Axis is Day of Treatment and the y Axis is the Percent of Eschar Out of the Initial Amount of Eschar:
This parameter shows the eschar removal efficacy of the treatments: the more effective the treatments are, the smaller the AUC. This parameter was found to be significantly dependent on the mount of API. The dependency on API amount was linear (
Taken together, these results indicated that the effect of API was does and time dependent.
Five treatment-blinded assessors scored each wound based on the photographs of the wounds through the entire experiment. The irritation caused by the placebo was found to be significantly lower than that of the treatment groups. Irritation was dependent on API amount and disappeared completely after a day or two in the follow up period in all treatments.
The aim of this study is to assess the safety and the efficacy of two doses: 2% (w/w) and 5% (w/w) of the gel formulation disclosed herein above in Example 1, also designated EX-02, compared to placebo in debridement of chronic venous leg ulcers and of diabetic lower extremity ulcers.
The study is a multicenter, prospective, randomized, placebo controlled, double-blind, international study.
Adults with >50% necrotic/slough/fibrin non-viable tissue on a chronic wound (venous leg ulcer, diabetic lower extremity ulcer) between 3 cm2 and 200 cm2 (surface area) are enrolled into the study.
Patients are randomized to EX-02 Low dose (2% w/w), EX-02 high dose (5% w/w), or Placebo treatment group. Treatment is performed three times a week up to 10 applications (up to 10 visits) or until complete debridement is achieved, whichever occurs first. The duration of each application is 24+2 hours or three times a week, namely 48+4 hours and 72+4 hours per application. Following each application the wound is washed, photographed and assessed for wound size and removal of nonviable tissue (by digital planimetry software) and change in granulation tissue, wound status, and safety parameters. The 24 hour treatments are performed successively during week days. During weekends the wound are dressed with moist-to-moist saline gauze.
Following completion of the debridement treatment period, patients are treated according to standard procedures and evaluated (wound assessments) once a week until complete wound closure for up to 12 weeks from last application (up to 12 visits). For patients who achieved wound closure, additional 3 monthly follow-up visits of wound closure confirmation is conducted; the first monthly visit is performed 2 weeks after reaching wound closure. For patients who did not achieve wound closure during the 12-weeks follow-up visits, only the 3-months follow-up visit (week 30) is conducted. The placebo is prepared as a powder of the excipients only and water for the preparation of a gel.
The following endpoints are evaluated and compared between EX-02 and Placebo for all wounds:
Incidence of complete debridement (non-viable tissue removal) at the end of the debridement period (up to 8 treatment days).
Promotion of granulation tissue formation by the gel composition-Clinical study
Multi-center Phase 2 clinical trial (MW2017-06-28) compared EscharEx 5% (5% API in the composition, as detailed in example 1) (EX-02) debridement treatment to Gel Vehicle (GV) (composition as in EscharEx, excluding API) and non-surgical Standard of Care (NSSOC) in 119 patients (EscharEx-46 patients, GV-43 patients and NSSOC-30 patients) with Venous Leg Ulcers (VLU) with wound size of 2-100 cm2 and wound age between 4 weeks to 2 years. The NSSOC arm included enzymatic agents, alginates, foams, highly absorbents, honey, hydrocolloids, hydrofibers, hydrogels, and silver products, that were applied on the wound surface according to their approved label and instructions for use, at the investigator/clinician discretion, in accordance with ulcer status until a complete debridement was achieved. Compression wraps were mandatory.
Each subject was screened within 7(+2/−1) days, and randomized at the end of the screening period (using a computer-generated randomization stratified by region, pain level and wound size). In the daily visits period, each subject was treated with EX-02, Gel Vehicle or NSSOC, in accordance with the randomization arm, in a treatment module of 24+3 hours successive treatments of up to 8 applications, or until a complete debridement of the wound was achieved within 14 days, whichever came first.
Applications of EX-02 or Gel vehicle were restricted to daily visits period, while patients allocated to NSSOC arm continued debridement with NSSOC also in the following twice-weekly and weekly visits periods, as needed.
For patients of all study arms, daily visits period was followed by a twice-weekly visits period (4 visits performed twice a week, within 14 days) and thereafter by once weekly visits period (up to 10 visits performed once weekly, within up to 10 weeks), in which evaluations of wound management and closure follow-up were performed. Wound closure was confirmed within two consecutive study visits 2 weeks apart.
Granulation tissue was assessed clinically (visual assessment) by the principal investigator/clinician at bedside.
As can be seen in
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A time-dependent association between complete WBP and time to wound closure (WC) was demonstrated, suggesting strong time dependent correlation between WBP and time to WC. The Hazard Ratio (HR) of bed preparation (BP), indicator for achieving WC, was as high as 11.96 (95% CI=4.236 to 33.787, p<0.0001).
As can be seen in
Moreover, the probability of wounds to close is significantly lower if WBP was not achieved during the entire time of the study.
Accordingly, when treated by the compositions of the present invention, at least about 2.5 to 4 times the number of patients achieve wound bed preparation within the treatment time and those patients have a much greater possibility of wound closure and healing.
Notably, the compositions of the present invention were superior to other enzymatic debridement compositions or placebo controls in the ability to promote granulation. As can be seen in
Multi-center Phase 2 clinical trial (MW2017-06-28) (as described in example 4) compared 46 patients treated with EscharEx 5% (5% API in the formulation, as detailed in example 1) to 8 patients in the NSSOC arm treated with collagenase ointment (Santyl®, Smith-Nephew, Fort Worth, Texas), an enzymatic debridement formulation commonly used in clinical practice for the management of chronic wounds, particularly in cases where there is necrotic tissue or eschar present.
Table 6 presents a summary of the findings from a sub-group analysis conducted on the results of a phase 2 study involving patients with VLU. The data provides a direct comparison between EscharEx and Santyl®.
The results presented in Table 6 show the superiority of EscharEx over the commercial collagenase-based formulation both in achieving complete debridement as well as wound bed preparation. This signifies a groundbreaking therapeutic advancement that has the potential to change the treatment paradigm in chronic wounds.
It will be appreciated by persons skilled in the art that the present invention is not limited by what has been particularly shown and described herein above. Rather the scope of the invention is defined by the claims that follow.
This application is a continuation-in-part of application Ser. No. 16/094,384 filed Oct. 17, 2018, which is the US national stage filing of International Application PCT/IL2017/050110 filed Jan. 30, 2017 which claims the benefit of U.S. provisional application No. 62/323,812 filed Apr. 18, 2016. The entire content of each earlier filed application is expressly incorporated herein by reference thereto.
| Number | Date | Country | |
|---|---|---|---|
| 62323812 | Apr 2016 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16094384 | Oct 2018 | US |
| Child | 18657124 | US |
