Methods of Detection of Iron Deficiency

Abstract

New diagnostic indexes and the method of use for detection of iron deficiency have been disclosed. These include a logistic function of mean cell hemoglobin (MCH) and mean cell volume (MCV), and a logistic function of mean cell hemoglobin concentration (MCHC) and MCH.

Description

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 is the ROC curve of IDI₁, as described in Example 1.

FIG. 2 is the ROC curve of IDI₂, as described in Example 2.

Claims

1. A method of detection of iron deficiency comprising the steps of: (a) analyzing a blood sample on a hematology analyzer and obtaining mean cell hemoglobin (MCH) and mean cell volume (MCV) of red blood cells;(b) obtaining an iron deficiency index (IDI1) defined as a function of said MCH and said MCV;(c) comparing said IDI1 to a predetermined IDI1 iron deficiency criterion; and(d) reporting an indication of iron deficiency if said IDI1 meets said predetermined IDI1 iron deficiency criterion.

2. The method of claim 1, wherein said IDI1 is a logistic function of said MCH and said MCV, defined by following equation: IDI1=exp(a+b*MCV+c*MCH)/(1+exp(a+b*MCV+c*MCH))wherein a, b and c are constants.

3. The method of claim 2, wherein said a, b and c are −35.6, 0.087 and 1.61, respectively.

4. The method of claim 1, wherein said iron deficiency comprises absolute iron deficiency or functional iron deficiency.

5. A method of detection of iron deficiency comprising the steps of: (a) analyzing a blood sample on a hematology analyzer and obtaining mean cell hemoglobin concentration (MCHC) and mean cell hemoglobin (MCH) of red blood cells;(b) obtaining an iron deficiency index (IDI2) defined as a function of said MCHC and said MCH;(c) comparing said IDI2 to a predetermined IDI2 iron deficiency criterion; and(d) reporting an indication of iron deficiency if said IDI2 meets said predetermined IDI2 iron deficiency criterion.

6. The method of claim 5, wherein said IDI2 is a logistic function of said MCH and said MCHC, defined by following equation: IDI2=exp(i+j*MCH+k*MCHC)/(1+exp(i+j*MCH+k*MCHC))wherein i, j and k are constants.

7. The method of claim 6, wherein said i, j and k are −97.98, 0.24 and 2.91, respectively.

8. The method of claim 5, wherein said iron deficiency comprises absolute iron deficiency, or functional iron deficiency.

9. A method of producing an iron deficiency index (IDI1) of a blood sample on a hematology analyzer comprising: (a) mixing a first aliquot of a blood sample with a blood diluent to form a first sample mixture, analyzing said first sample mixture on said hematology analyzer, and obtaining a mean cell volume of red blood cells (MCV) and a red blood cell concentration (RBC);(b) mixing a second aliquot of said blood sample with a reagent system to form a second sample mixture, analyzing said second sample mixture on said hematology analyzer, and obtaining a hemoglobin concentration (Hgb) of said blood sample;(c) obtaining mean cell hemoglobin (MCH) using said RBC and said Hgb;(d) obtaining said iron deficiency index (IDI1) using said MCV and said MCH, said IDI1 being defined as a logistic function of said MCV and said MCH; and(e) reporting said IDI1 of said blood sample on said hematology analyzer.

10. The method of claim 10, wherein said IDI1 is defined by following equation: IDI1=exp(a+b*MCV+c*MCH)/(1+exp(a+b*MCV+c*MCH))wherein a, b and c are constants.

11. The method of claim 10, wherein said a, b and c are −35.6, 0.087 and 1.61, respectively.

12. A method of producing an iron deficiency index (IDI2) of a blood sample on a hematology analyzer comprising: (a) mixing a first aliquot of a blood sample with a blood diluent to form a first sample mixture, analyzing said first sample mixture on said hematology analyzer, and obtaining a mean cell volume of red blood cells (MCV) and a red blood cell concentration (RBC);(b) mixing a second aliquot of said blood sample with a reagent system to form a second sample mixture, analyzing said second sample mixture on said hematology analyzer, and obtaining a hemoglobin concentration (Hgb) of said blood sample;(c) obtaining mean cell hemoglobin (MCH) and mean cell hemoglobin concentration (MCHC) using said MCV, said RBC and said Hgb;(d) obtaining said iron deficiency index (IDI2) using said MCH and said MCHC, said IDI2 being defined as a logistic function of said MCH and said MCHC; and(e) reporting said IDI2 of said blood sample on said hematology analyzer.

13. The method of claim 12, wherein said IDI2 is defined by following equation: IDI2=exp(i+j*MCH+k*MCHC)/(1+exp(i+j*MCH+k*MCHC))wherein i, j and k are constants.

14. The method of claim 13, wherein said i, j and k are −97.98, 0.24 and 2.91, respectively.