Claims
- 1. A method of identifying a compound that binds to a prosaposin receptor comprising:
(a) incubating a first test compound with said receptor; and (b) determining whether said first test compound binds to said receptor.
- 2. The method of claim 1, wherein said receptor is isolated by passage of a solubilized neural plasma membrane P-100 fraction through an affinity column comprising amino acids 8-29 of saposin C.
- 3. The method of claim 1, wherein said receptor is coated onto a surface or is placed on a conducting surface.
- 4. The method of claim 1, wherein said first test compound is a protein, polypeptide, peptide, peptidomimetic, small organic molecule or organomimetic.
- 5. The method of claim 1, wherein said first test compound is contained within a library of proteins, polypeptides, peptides, peptidomimetics, small organic compounds or organomimetics.
- 6. The method of claim 1, wherein said determining step comprises a competition assay.
- 7. The method of claim 1, further comprising the steps of repeating steps (a) and (b) with a second test compound.
- 8. The method of claim 1, further comprising the step of performing a functional assay to ascertain whether said first test compound identified in steps (a) and (b) has agonist or antagonist function.
RELATED APPLICATIONS
[0001] This application is a continuation and claims priority to U.S. Patent Application No. 08/896,181, filed on Jul. 17, 1997, which is herein expressly incorporated by reference in its entirety.
Continuations (1)
|
Number |
Date |
Country |
Parent |
08896181 |
Jul 1997 |
US |
Child |
09785935 |
Feb 2001 |
US |