Patent Application
20200375469
Dental enamel is a thin, hard layer of calcified material that covers the crown of teeth. The major mineral component of dental enamel is hydroxyapatite, a crystalline form of calcium phosphate. Poorly mineralized regions beneath, within or adjacent to intact enamel often appear as white spots on the teeth because such poorly mineralized regions are porous and reflect light differently from enamel. In most cases, white spots are the result of demineralization, for example from acids produced by cariogenic bacteria, which break down the hydroxyapatite into calcium and phosphate ions, leading to porous regions within the enamel. Other common causes of poor mineralization and associated white spot lesions include xerostomia, trauma, and arrested decay that has only partially remineralized around fixed orthodontic appliances, which may provide shelter for bacteria or interfere with normal remineralization. Conditions that interfere with tooth development, such as fluorosis, hypomineralization/hypomaturation, mineral deficiencies, and hypoplasia, can also cause white spot lesions.
The diagnosis and evaluation of white spots is typically performed simply by looking at the teeth. The development of the white spots and/or the efficacy of treatments to remove white spots typically judged simply by looking at “before” and “after” pictures. Such evaluation is subjective and qualitative. The results depend to a large extent on the person making the assessment and the lighting conditions when the assessment is made.
It is possible to measure and compare grayscale values of teeth in a photograph, but the contrast between the white spot and the surrounding white enamel using conventional grayscale values is generally inadequate to provide a degree of distinction sufficient to measure fine changes or differences in the white spots and is subject to distortion by the lighting conditions.
There is a need for improved methods of quantifying the severity of white spot lesions on the teeth, to allow more accurate assessment and monitoring of the white spots.
The present inventors have developed a new method of quantifying the severity of white spot lesions on the teeth.
Color digital images are made of pixels, a pixel being the smallest controllable element of a digital picture. Pixels in a color digital image are made of combinations of primary colors represented by a series of code. Grayscale is a measure of intensity. A “channel” in this context is the grayscale image of the same subject as a color image, made of just one of these primary colors. For instance, an image from a standard digital camera will have a red, green and blue channel. CMYK images for printing have four channels for cyan, magenta, yellow and black ink. The digital code for each pixel therefore provides an address (where in the image it is located) and a grayscale (intensity value) for each channel. The blend of the intensity of each channel in each pixel and the blend of pixels in the picture all combine to provide the image that we see.
This disclosure provides methods of quantifying the severity and monitoring the progression of white spot lesions on a tooth by quantifying the grayscale difference between the white spot lesions and the surrounding healthy enamel in the blue channel of a digital color photographic image of the tooth. Focusing on the contrast in the blue spectrum highlights the differences between the white spot areas and the sound enamel (which is naturally slightly yellow and therefore absorbs blue light) and digitally filters out much of the variability generated by different lighting in different environments, providing a more accurate and consistent measure of the severity of the white spot relative to the adjacent enamel. The blue channel is the channel corresponding to a color with a wavelength in the range of 450-520 nm, e.g., a color corresponding to about 450-495 nm, e.g. about 470 nm.
For example, in one embodiment, the disclosure provides a novel method for quantifying the severity of white spots on teeth, comprising
The difference between the the grayscale value of the pixel in the area depicting the white spot and the grayscale value of the pixel in the area depicting the adjacent healthy enamel is a measure of the contrast between the white spot and the new adjacent enamel. The greater the contrast, the more noticeable and/or severe the white spot is. If the contrast increases over time, that indicates the white spot is getting worse. If the contrast reduces, that indicates the severity of the white spot is reduced, at least from an aesthetic perspective.
In another embodiment the disclosure provides a method of evaluating a treatment for white spot on the teeth of a patient comprising quantifying the severity of white spots on teeth as described above before and after such treatment, wherein a reduction in the difference between the grayscale value of at least one pixel in the area depicting at least a portion of the white spot and the grayscale value of at least one pixel in the area depicting at least a portion of the adjacent healthy enamel indicates the efficacy of such treatment.
For example, the white spot repair efficacy of a proposed treatment can be calculated as follows:
In another embodiment the disclosure provides a method of monitoring the progress of a white spot condition on the teeth of a patient comprising quantifying the severity of white spots on teeth over time, as described above, wherein a reduction in the difference between the grayscale value of at least one pixel in the area depicting at least a portion of the white spot and the grayscale value of at least one pixel in the area depicting at least a portion of the adjacent healthy enamel indicates repair of the white spot.
Further areas of applicability of the present invention will become apparent from the detailed description provided hereinafter. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Digital cameras and image processing software are widely available, and virtually all image processing programs permit shifting of color balance, which would enable them to be used for the methods of the instant disclosure.
For example, ImageJ is a public domain, Java-based image processing program developed at the National Institutes of Health, which can be downloaded free at https://imagej.nih.gov/ij/. Using ImageJ, the operations can be carried out as follows:
The following examples illustrate methods for use in accordance with the present disclosure. The exemplified methods are illustrative and do not limit the scope of the disclosure.
Human extracted teeth with white spot(s) are gently brush cleaned and also screened by dentist. The screened teeth are then stored in deionized water at ca. 4° C. until the treatments are started.
The stored teeth are taken out, slightly dried with paper towel before the base line images of the white spots and adjacent sound areas are taken. The contrast values between the white spot and adjacent sound areas are quantified using ImageJ.
The teeth are then soaked in one of three test solutions or control mouthwash for 30 minutes, 15 ml for each tooth, at 37° C., while being spun at 100 rpm. The teeth are then rinsed for 5 minutes with deionized water at 400 rpm. The teeth are then paper towel dried, and the deionized water rinse is repeated. The treated teeth are then incubated in artificial saliva overnight at 37° C.
This treatment, washing and incubation is repeated daily for nine more days, for a total of 10 days of treatment. After the 10 days of treatment is completed, the teeth are rinsed twice with deionized water as described.
In order to avoid false repair readings, e.g., decreased contrast from loosely deposited mineral (from artificial saliva) on tooth surface, the teeth are brushed using medium toothbrush after all treatment/incubation. The teeth are each brushed under tap water, continuously and evenly at all angles for two minutes, rinsed under tap water, and placed onto a paper towel to dry.
Final images of the treated teeth, after brushing, are taken after the teeth are slightly dried with the paper towel. The contrast between the white spot area and the adjacent sound enamel is again quantified. Reduction in contrast (negative value) indicates degree of repair of the white spot. The results are set forth in Table 2:
This data shows that the three novel treatment solutions reduced the white spot lesions, while the control treatment had little effect on the white spots.
While particular embodiments of the invention have been illustrated and described, it will be obvious to those skilled in the art that various changes and modifications may be made without departing from the scope of the invention as defined in the appended claims.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2018/029134 | 4/24/2018 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62489366 | Apr 2017 | US |
