1. Field of the Invention
The present invention relates to methods and related devices for supplying fluid flow to the venous system in a retrograde manner. For example, the methods and related devices allow retrograde oxygenated blood flow in a coronary vein, for example, by forming a passageway between a blood-containing anatomical structure, which may contain oxygenated blood, such as a left ventricle or coronary artery, for example, and the coronary vein and controlling internal pressure of the coronary vein during the retroperfusion by at least partially obstructing the coronary vein at a location upstream of the passageway.
2. Background of the Related Art
A prevalent form of heart failure involves the build-up of plaque on walls of various vascular structure, such as, for example, the coronary artery. The plaque that builds up on the walls can form either a partial or total occlusion in the artery. Such an occlusion may either limit or completely block blood flow through the artery, which typically enters the artery through the aortic valve from the left ventricle. Because the coronary artery supplies blood to the various blood vessels within the muscle forming the heart wall, limiting or blocking the blood flow through the coronary artery can result in damage to the heart muscle, such as, for example, ischemia. Ischemic tissue can lead to reduced cardiac function by diminishing the pumping capacity of the heart. In some instances, the diminished capacity of the heart can lead to heart attack.
Various techniques have been developed to treat this type of heart condition. For example, a surgical technique, referred to as coronary artery bypass grafting (CABG), involves removing a vein or portion thereof from the patient, usually from the femoral vein, and grafting the vein so as to connect portions of the coronary artery upstream and downstream of the occlusion. Thus, the blood flow is diverted around the occlusion and through the vein graft so that the oxygenated blood can be delivered to the vessels in the heart wall. CABG generally is performed as an open surgery resulting in relatively long recovery times. Patients also often experience a large amount of discomfort resulting from harvesting of the veins to be used in CABG. Furthermore, the veins that are grafted to the coronary artery have a limited useful life.
Coronary angioplasty represents another form of treatment of arteries having occlusions that can be performed as an alternative to bypass surgery. In this technique, a balloon catheter is inserted percutaneously into the coronary artery. Once the catheter has been inserted such that the balloon is adjacent the occlusion being treated, the balloon is inflated to dilate the artery in the location of the occlusion. Often this technique involves inflating and deflating the balloon repeatedly to establish the desired dilation of the artery. This technique may include placing a stent in a collinear manner in the artery at the location of the occlusion to maintain the proper dilation of the artery. Delivery of the stent can be accomplished by removing the dilation balloon catheter and then inserting a balloon catheter carrying the stent into the artery. A multiple balloon stent delivery catheter may dilate the artery and place the stent in a single insertion of the catheter into the patient.
Yet another technique includes delivering oxygenated blood to the heart wall in a retrograde manner by creating a passageway between a coronary artery and a coronary vein at a location upstream of the occlusion in the coronary artery. In this technique the coronary vein is typically ligated or otherwise blocked to prevent antegrade blood flow from the passageway into the coronary sinus. The arterial blood is thus introduced from the coronary artery, through the passageway, and into the coronary vein where it may flow in a retrograde manner to perfuse the heart wall. This retroperfusion technique (i.e. using retrograde flow in the venous system from the arterial system to perfuse the heart), however, may be associated with relatively high incidences of hemorrhagic infarct or tissue engorgement leading to cell death. Such problems may be caused at least in part by preventing antegrade blood flow into the coronary sinus. In other words, under certain conditions, performing retroperfusion without permitting the an outflow from the heart through the venous system may cause overperfusion of the heart.
Moreover, conventional techniques to retroperfuse the heart by delivering blood from a coronary artery to the coronary vein in a retrograde manner have not taken into account the internal pressure experienced by the coronary vein, which as explained above may have effects on both the vein itself as well as well as the ability to perfuse the heart. For example, completely blocking or ligating the coronary vein proximate the location of the passageway, in addition to causing overperfusion of the heart, may potentially damage the vein due to increased pressures in the vein above those found under normal conditions (i.e., in the absence of arterial retrograde blood flow).
On the other hand, however, to leave the vein unblocked may result in inefficient perfusion of the heart due to competition between the retrograde blood flow through the vein with the antegrade blood flow through the vein. Such competing flows may result in an the heart being underperfused.
It may thus be desirable to provide a technique for retroperfusing the heart that permits at least some antegrade blood flow through the venous system.
It also may be desirable to provide a retroperfusion technique that controls pressure in the vessel, for example, limiting pressure generated in the vessel, through which retrograde blood flow occurs. For example, it may be desirable to limit the pressure such that it is maintained substantially at or below a predetermined value or predetermined range of values, which may be selected based on a measured internal pressure of the coronary vein.
It should be understood that the invention could be practiced without performing one or more of the aspects, objects, or advantages described above. Other aspects will become apparent from the following detailed description.
An aspect of the invention includes a method of treating a heart that includes flowing blood through a passageway between a blood-containing anatomical structure, for example an oxygenated blood-containing anatomical structure, and a coronary vein so as to cause retrograde blood flow in the coronary vein. The method further includes measuring an internal pressure of the coronary vein to determine a measured internal pressure. The method may also include at least partially obstructing the coronary vein upstream, with respect to the retrograde blood flow, of the passageway based on the measured internal pressure.
At least partially obstructing may include (1) allowing at least some antegrade blood flow past the location throughout a cardiac cycle, (2) allowing at least some antegrade blood flow past the location during a portion a cardiac cycle and preventing antegrade blood flow past the location during another portion a cardiac cycle, or (3) completely obstructing the vessel so as to prevent antegrade blood flow past the location throughout a cardiac cycle.
Another aspect of the invention includes a method of treating a heart comprising flowing blood through a passageway between a heart chamber and a coronary vein so as to cause retrograde blood flow in the coronary vein and at least partially obstructing the coronary vein at a location upstream of the passageway relative to a direction of the retrograde blood flow.
Yet another aspect of the invention includes a system for use in retroperfusing a heart by causing retrograde blood flow in a coronary vein. The system may include a pressure measuring device configured to measure the internal pressure of a coronary vein. The system also may include an implant configured to be placed relative to the coronary vein at a location upstream, relative to a direction of the retrograde blood flow, of a source producing the retrograde blood flow. The implant may be configured to at least partially obstruct the coronary vein.
Yet another exemplary aspect of the invention includes a device for use in retroperfusing a heart by causing retrograde blood flow in a coronary vein. The device includes an implant configured to be placed within the coronary vein at a location upstream, relative to a direction of the retrograde blood flow, of a passageway between the coronary vein and a heart chamber. The implant may be configured to at least partially obstruct the coronary vein.
Aside from the structural and procedural arrangements set forth above, the invention could include a number of other arrangements, such as those explained hereinafter. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention.
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification. The drawings illustrate exemplary embodiments of the invention, and together with the description, serve to explain certain principles. In the drawings,
The present invention pertains to methods, and related devices for performing the methods, of retroperfusing a heart by flowing oxygenated blood in the venous system in a retrograde manner. By way of example, the disclosed methods and devices may be used to bypass a total or partial occlusion of a coronary vessel by forming a passageway between a heart chamber (e.g., left ventricle) and a vein (e.g., coronary vein) or between an artery (e.g., coronary artery) and a vein (e.g., coronary vein) and allowing retrograde blood flow in the vein to at least a portion of a heart wall. The techniques using the methods and devices may be performed either surgically or via a less invasive technique, such as percutaneous approaches, for example.
The method may generally include flowing blood through a passageway between a coronary vein and an oxygenated blood-containing anatomical structure so as to allow retrograde blood flow in the coronary vein and controlling the internal pressure of the coronary vein during the retroperfusion by at least partially obstructing the coronary vein at a location upstream of the passageway. When used herein, the terms “upstream” and “downstream” are relative to the direction of the retrograde blood flow in the vein, unless otherwise indicated. The method may include measuring an internal pressure of the coronary venous system via a pressure measuring device and at least partially obstructing the coronary vein based on the measured internal pressure. The at least partially obstructing the coronary vein based on the measured internal pressure may include, among other things, selecting an amount of obstruction and/or location of the obstruction.
By way of example, the internal pressure that is measured in the coronary venous system is the wedge pressure, and more particularly, the mean wedge pressure, for example.
Although the methods and devices according to exemplary aspects of the present invention will be described with reference to retroperfusing a heart by forming a passageway between an oxygenated blood-containing anatomical structure (e.g., a heart chamber, such as a left ventricle, or a coronary vessel, such as a coronary artery) and the coronary vein to allow retrograde blood flow in the coronary vein, the methods and devices described may be practiced in various other settings, aside from the heart, to provide blood flow to a target area in a retrograde manner. Further, oxygenated blood-containing anatomical structures may be any such structure, including other heart chambers, other coronary vessels, and any other oxygenated blood-containing anatomical structures. In addition, the source of oxygenated blood for providing the retrograde blood flow to the coronary vein may be any other type of source, whether natural or artificial.
To facilitate the retrograde blood flow 28, the coronary vein CV may be at least partially obstructed at a location 26, as illustrated in
As illustrated in
In accordance with an exemplary aspect, the internal pressure of the coronary vein CV during retroperfusion may be controlled. The internal pressure of the coronary vein CV during retroperfusion should be high enough to cause adequate retrograde blood flow to the ischemic region of the heart being treated. For example, the pressure should be such that the retrograde blood flow is sufficient to overcome the antegrade blood flow through the vein so that the heart is not underperfused. At the same time, the internal pressure of the coronary vein CV during retroperfusion should not be too high so as to cause overperfusion of the heart and/or potential damage to the coronary vein CV. An optimal value, or a range of values, for the internal pressure of the coronary vein CV during retroperfusion may vary depending on the particular patient, the particular coronary vein in which the retroperfusion is to be performed, and the particular site of the retroperfusion in the coronary vein.
Accordingly, the internal pressure, such as the mean wedge pressure, for example, of the coronary venous system may be measured, and an amount of obstruction of antegrade flow in the vein may be tailored for a particular patient and selected based on the measured pressure so as to achieve and maintain as closely as possible a predetermined pressure or range of pressures. The mean wedge pressure of the coronary venous system may be measured before establishing the retrograde blood flow therethrough, for example, before forming the passageway 22. Alternatively, the mean wedge pressure may be measured after forming the passageway 22. An exemplary method of measuring mean wedge pressure of the coronary vein CV and determining a target pressure range for the coronary vein while establishing retrograde flow in the vein is disclosed in U.S. Pat. No. 6,458,323 to Boekstegers. The entire disclosure of U.S. Pat. No. 6,458,323 is incorporated herein by reference.
By way of example, pressure measurement may be performed by inserting a balloon or other type of occluder into the vein and measuring the pressure via a pressure transducer or pressure-measuring guidewire distal to the occluder. Such pressure measurement techniques would be obvious to those skilled in the art. As mentioned above, it also is contemplated that the pressure could be measured after formation of the passageway, in which case the mean wedge pressure could be measured using similar techniques in order to determine a desired amount and/or location of the obstruction.
In addition to the amount of obstruction, the location of the obstruction may be selected to control the internal pressure of the coronary vein CV during retroperfusion. For example, given the same amount of obstruction, selecting the location of the obstruction closer to the passageway 22 may reduce the internal pressure of the coronary vein CV during retroperfusion. Accordingly, the internal pressure of the coronary vessel during retroperfusion may be controlled by selecting the amount of the obstruction, selecting the location of the obstruction, or selecting the combination of the amount and the location of the obstruction.
Aside from the amount of obstruction and/or location of the obstruction, other mechanisms, such as pressure absorption mechanisms, for example, also may be used to control the internal pressure of the coronary vein. Examples of such mechanisms for controlling the internal pressure of the coronary vein are described in more detail below.
According to an exemplary aspect, the coronary vein CV may be completely obstructed at the location 26 so as to prevent antegrade blood flow past the location 26 throughout a cardiac cycle.
The completely-obstructing implant 35 may be self-expandable. It may be delivered to the location 26 via a catheter or sheath (not shown) in a collapsed state and may be pushed out of the catheter or sheath into the lumen 25 of the coronary vein CV. Upon being pushed out of the catheter or sheath, the completely-obstructing implant 35 may expand to engage its external surface 37 with the inner surface 27 of the coronary vein CV. Alternatively, the implant may be balloon expandable and delivered via a balloon catheter, for example.
It is envisioned that the implant 35 may be a substantially hollow structure or it may be a solid structure. In either case, the implant 35 has an end face 39 which blocks flow through the implant 35. Skilled artisans would understand that the impermeable end face 39 could be positioned at either or both ends of the implant 35.
To help secure the implant 35 within the lumen 25 at the location 26, an attachment mechanism may be provided on the implant 35. As shown in
The deformation of the end face 49 may provide a mechanism by which to control the internal pressure of the coronary vein CV during retroperfusion. Accordingly, if the completely-obstructing implant 45 with the flexible end face 49 is used, the internal pressure of the coronary vein CV during retroperfusion may be controlled by selecting the location 26 relative to the passageway 22 and/or selecting an appropriate flexibility, and thus degree of deformation, of the end face 49. Like the exemplary embodiment of
Yet another exemplary embodiment of a device for obstructing the coronary vein through which retrograde blood flow occurs is shown in
Alternatively, or in addition to, the magnetic elements 52 may be placed in or attached to the walls of the coronary vein CV (not shown). The number and/or the magnetic strength of the magnetic elements 52 may be appropriately selected to ensure that the coronary vein CV is either partially or completely collapsed at the location 26, as desired. Accordingly, if the number and/or the magnetic strength of the magnetic elements 52 are selected to completely collapse the coronary vein CV (
Exemplary magnetic elements and methods of placing magnetic elements into body structures are disclosed in U.S. Patent Application Publication No. 2000/0091295 A1, entitled “Medical Treatment Method and Device Utilizing Magnetic Particles.” The entire disclosure of U.S. Patent Application Publication No. 2000/0091295 A1 is incorporated herein by reference.
As mentioned above, rather than being completely obstructed at the location 26, the coronary vein CV may be partially obstructed at the location 26 so as to allow at least some antegrade blood flow past the location 26 during at least a portion of a cardiac cycle. For example, a partial obstruction may allow at least some antegrade blood flow past the location throughout a cardiac cycle. Alternatively, a partial obstruction may allow at least some antegrade blood flow past the location during a portion of a cardiac cycle and prevent antegrade blood flow past the location during another portion of the cardiac cycle.
To control the collapsing of the vein in conjunction with the various portions of the cardiac cycle, the magnetic elements 132 may be influenced by electrical signals associated with the heart during the various portions of the cardiac cycle, for example. Alternatively, an externally controlled magnetic source (not shown) may be coupled to the magnets 132 and may either automatically or manually be activated to cause the magnetic strength of the magnets 132 to change depending on the desired degree of collapsing of the vein during a particular portion of the cardiac cycle. Other means for controlling the degree of collapse of the vein and timing the collapsing of the vein with portions of the cardiac cycle may also be used and are considered within the scope of this invention.
Accordingly, in addition to the location 26, the number and/or the magnetic strength of the magnetic elements 132, in addition to altering the magnetic strength depending on the phase of the cardiac cycle, may be selected to control the internal pressure of the coronary vein CV during retroperfusion. As described above, these various features to control the internal pressure of the coronary vein CV during retroperfusion may be selected based on the measured internal pressure of the coronary vein CV before or after forming the passageway 22.
The partially-obstructing implant 65 further defines a passage 66 configured to allow at least some antegrade blood flow 29 therethrough. This passage 66 may be configured to allow some antegrade blood flow therethrough throughout a cardiac cycle. Alternatively, the passage 66 may be configured to close during at least part of the cardiac cycle thereby allowing antegrade blood flow through the passage 66 during only part of the cardiac cycle. For example, the passage 66 may be configured to close (for example, by collapse of the implant 65) during systole and open to permit antegrade blood flow therethrough during diastole. Such deformation may occur due to the structure of the implant 60 itself, which may take into account the various forces experienced by the vein during the cardiac cycle and be configured so as to collapse and open, respectively, in conjunction with those forces. Alternatively, an external source may be used to control the collapsing and opening of the implant. Exemplary embodiments of such devices to control the collapse and opening of an implant having similar structures to the implant 60 are described below with reference to
In the illustrated embodiment of
The partially-obstructing implant 65 may be self-expandable. Alternatively, the partially-obstructing implant 65 may be expandable by a dilation mechanism, such as a balloon. A balloon (not shown) disposed within the passage 66 may be expanded in the lumen 25 of the coronary vein CV to expand the partially-obstructing implant 65. The partially-obstructing implant 65 may further include an attachment mechanism, for example, in the form of a plurality of prongs 40 extending from the external surface 67, or other suitable mechanisms.
Another exemplary embodiment of a blocking device in the form of an implant defining a passage with a non-uniform cross-section is shown in
These tapered configurations may provide another mechanism to control the internal pressure of the coronary vein CV during retroperfusion. Different tapered configurations may be selected so as to provide different flow resistances through the passage, for example. Accordingly, in addition to the various features explained above, a degree and/or configuration of the taper and/or passage cross-section may be used as yet another mechanism to control the internal pressure of the coronary vein CV during retroperfusion.
As with the embodiment of
Yet another exemplary embodiment of a blocking device for partially obstructing the coronary vein at a location so as to allow at least some antegrade blood flow past the location during a cardiac cycle is shown in
As with the embodiments discussed above, various configurations of the cone 95, such as the porosity of the covering of the lateral surface, the size of the openings at each end 98, 99, the degree of taper of the passage 22, etc. may be altered so as to obtain a desired antegrade flow therethrough and thereby control the pressure within the coronary vein CV.
The end face 119 closest to the passageway 22 may be rigid so that it does not deform throughout the cardiac cycle. Alternatively, as described with respect to
The size of the space 116 may be varied by selecting different biasing strengths for the biasing member 118. Accordingly, in addition to the various features explained above, the biasing strength of the biasing member 118 may be selected to control the internal pressure of the coronary vein CV during retroperfusion.
As described above with respect to the embodiments of the blocking devices of
The partially-obstructing implant 145 includes a lumen 146 extending between the two ends 148, 149 and a biasing member 144, such as, for example, a relatively thin elastic or inelastic band, may be disposed around the external surface 147. For example, the biasing member 148 may be disposed around a middle portion of the implant 145. The biasing strength and elasticity of the biasing member 144 may be selected such that it causes the lumen 146 to open during a first portion of the cardiac cycle, as shown in
The partially-obstructing implant 145 may be self-expandable. Alternatively, the partially-obstructing implant 145 may be balloon-expandable and/or expandable by other dilation mechanisms. The biasing member 144 may be elastic or inelastic. Also, in the case of a balloon-expandable implant, the degree of closure may be controlled by making the biasing member 144 more compliant under different balloon expansion pressures. In other words, the biasing member 144 may be configured such that its elasticity or compliance have different values depending on the different pressure applied to expand the implant 145. This may permit the biasing member 144 to be elastic so as to expand to a certain degree and then to be substantially inelastic, i.e., resist further expansion, once expanded to the desired degree. The partially-obstructing implant 145 may further include an attachment mechanism, such as, for example, a plurality of prongs 40 extending from the external surface 147.
As illustrated in
As described above, the biasing member 144 may be elastic and made from materials such as elastomers, rubbers, silicones, expandable metals, and other materials exhibiting elastic behavior. Alternatively, the biasing member 144 may be substantially inelastic and made from materials such as, for example, metals, such as stainless steel, for example, rigid plastics, such as polyethylene, for example, and other substantially inelastic materials. In an exemplary embodiment, the biasing member 144 may be in the form of a suture encircling the implant.
According to an exemplary embodiment, the stent 101 may have at least two differing stent cell structures. For example, a first stent cell structure 102′ may make up the restricted region 102 of the stent 101, as shown in
The specific stent cell structures shown in
Instead of forming the differing stent cell structures from a single piece tube, as described above with reference to
Stent 101 may be made of materials such as, for example, stainless steel, shape memory alloys, metals, plastics, and other materials used for making stents. In an exemplary embodiment, the stent may be provided with a covering 107, as shown in
As with other exemplary embodiments described herein, the implant of
Further, it is contemplated that the restricted region 102 of the stent 101 may be such that the lumen of the stent 101 is completely or substantially collapsed during either a portion of or throughout the cardiac cycle. For example, in the case of the latter, the stent 101 may be a self-expandable stent, with the restricted region 102 being nonexpandable such that the lumen remains completely or substantially collapsed at that region upon deployment of the stent 101. In the case of the former, the stent structure 102′ at the restricted region 102 may be configured so as to provide some expansion to open the stent lumen during a portion of the cardiac cycle while closing or substantially closing the stent lumen during another portion of the cardiac cycle. As an example, the expansion of the restricted region may occur by providing a stent structure that responds to various forces associated with the cardiac cycle.
The partially-obstructing implant 165 defines a passage 166 extending between the open ends 168, 169. One or more inflatable members 164 may be disposed adjacent the external surface 167 of the implant. The inflatable members 164 may have a variety of forms, such as, for example, balloons. An exemplary form of the inflatable members 164 may be a pocket 163 containing an air bubble 162, as shown in
Accordingly, in addition to the various features explained above, any combination of the number, size, and distribution of the air bubbles 162 and/or the pockets 163 may be selected to control amount of occlusion of the coronary vein CV, thereby controlling the amount of antegrade flow through the passage 166 and the internal pressure of the coronary vein CV during retroperfusion. As described above, these features to control the internal pressure of the coronary vein CV during retroperfusion may be selected based on the measured internal pressure of the coronary vein CV before or after forming the passageway 22.
The partially-obstructing implant 165 may be self-expandable. Alternatively, the partially-obstructing implant 165 may be expandable by a dilation mechanism. For example, a balloon disposed within the passage 166 may be expanded in the lumen 25 of the coronary vein CV to expand the partially-obstructing implant 165. The partially-obstructing implant 165 may also be provided with an attachment mechanism, such as the plurality of prongs 40 shown in
As illustrated in
As discussed, the various exemplary embodiments of blocking devices described above may be used to treat the heart via retroperfusion. In an exemplary aspect, the wedge pressure, for example the mean wedge pressure, of the coronary vein of a patient may be measured, for example prior to forming a passage between an oxygenated blood-containing anatomical structure and the coronary vein to establish retrograde flow in the coronary vein. Based on the measured wedge pressure, a target pressure or range of pressures may be determined. For example, the target pressure or range of pressures may be selected so that the pressure in the coronary vessel is high enough to cause retroperfusion but low enough so that the heart is not overperfused and/or so that the risk of damage to the vessel due to excess pressure is minimized. Based on the measured pressure and/or the target pressure or range of pressures, a blocking device may be selected so as to control the pressure in the coronary vein to approximately the target level. Factors such as the amount of obstruction, location of the obstruction, and/or amount of pressure absorption also may be determined based on the measured pressure so as to control pressure in the coronary vessel to the predetermined target level and those factors may be used to determine which of a plurality of blocking devices having differing characteristics to select for implantation. In this way, for each patient, the type and/or location of the blocking device used may be tailored to meet that patient's particular needs, thereby optimizing treatment of the patient.
An exemplary embodiment of a system for use in retroperfusing a patient's heart may therefore include a pressure measuring device for measuring the wedge pressure of the coronary vein and at least one implant configured to be positioned relative to the coronary vein and to at least partially block antegrade flow in the coronary vein. As an example, such a system may include a plurality of implants, for example in the form of the various blocking devices described herein, and each of the implants may have differing characteristics so that they partially obstruct the flow in differing ways.
It should be understood that the various embodiments described herein are exemplary only. Various other structural configurations may be envisioned and provide the same function for at least partially obstructing antegrade blood flow through the coronary vein. Further, embodiments of blocking devices that have been described as permitting at least some antegrade blood flow past the location also may be configured so as to completely block the coronary vein CV so as to prevent antegrade blood flow past the location of the blocking device throughout the cardiac cycle.
Additionally, in its broadest aspects, the invention is not limited to retroperfusing a heart in the manner described with reference to the exemplary embodiments. The devices and methods described may be used to control fluid flows and pressures in a variety of other settings, as will be apparent to those skilled in the art. For example, the devices and methods may be useful to retroperfuse organs and tissue regions other than those associated with the heart. Further, the devices and methods have been discussed in conjunction with retroperfusing the venous system via oxygenated blood-containing anatomical structures. However, it is envisioned that the devices and methods also may be used in conjunction with retroperfusing the venous system via sources other than oxygenated blood-containing anatomical structures. Such sources may include, for example, artificial sources or external sources which can be placed in flow communication with the venous system to provide retrograde flow therethrough. In addition, the devices and methods described herein may find use in the field of flow control during the perfusion of various organs and other portions of the body, in which case the various partial blocking devices and methods may be used in conjunction with the arterial system to control blood flow and/or pressures therein.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. The specification and examples are exemplary only and the present invention is intended to cover modifications and variations.