Claims
- 1. A method of screening a compound for bioactivity, comprising
contacting a candidate bioactive compound with an organized tissue, and measuring in at least a cell of the organized tissue a biological parameter that is associated with bioactivity, wherein a change in the biological parameter that occurs as a result of said contacting step is indicative of bioactivity of said candidate compound.
- 2. The method of claim 1 wherein said contacting step comprises contacting a candidate bioactive compound with an organized tissue comprising substantially post-mitotic cells.
- 3. The method of claim 1 wherein said contacting step comprises contacting a candidate bioactive compound with an organized tissue comprising cells aligned substantially parallel to each other and along a dimension of the vessel in which the cells were grown.
- 4. The method of claims 1-3 wherein at least a subset of cells of said organized tissue contain a foreign DNA sequence.
- 5. The method of claim 4 wherein the cells containing the foreign DNA sequence produce a substance of a type that is not normally present in the cells or in an amount that is not normally produced by the cells.
- 6. The method of claim 1 wherein said contacting step comprises contacting a candidate bioactive compound with an organized tissue comprising muscle cells.
- 7. The method of claim 2 wherein said substantially post-mitotic cells are muscle cells.
- 8. The method of claim 3 wherein said cells aligned substantially parallel to each other are muscle cells.
- 9. The method of claim 6 wherein said biological parameter comprises protein degradation.
- 10. The method of claim 4 wherein said foreign DNA sequence is a reporter gene encoding a detectable protein.
- 11. The method of claim 10 wherein said measuring step comprises detecting said detectable protein.
- 12. A method of screening a compound for bioactivity, comprising
(A) administering a candidate bioactive compound to a host organism in which an organized tissue is implanted; (B) removing at least a subset of cells of the organized tissue from the host organism; and (C) measuring in said cells of step (B) a biological parameter that is associated with bioactivity, wherein a change in the biological parameter that occurs as a result of said contacting step is indicative of bioactivity of said candidate compound.
- 13. A method of screening a compound for bioactivity, comprising
(A) administering a candidate bioactive compound to a host organism in which an organized tissue is implanted; (B) measuring in at least a cell of the organized tissue a biological parameter that is associated with bioactivity, wherein a change in the biological parameter that occurs as a result of said contacting step is indicative of bioactivity of said candidate compound.
- 14. A method of screening a compound for bioactivity, comprising
(A) administering a candidate bioactive compound to a host organism in which an organized tissue is implanted; (B) measuring in the host organism a biological parameter that is associated with bioactivity, wherein a change in the biological parameter that occurs as a result of said contacting step is indicative of bioactivity of said candidate compound.
- 15. The method of claim 14 wherein said measuring step is performed on a body fluid sample of said host organism.
- 16. A kit comprising a plurality of organoids wherein each organoid is individually contained in a physiological medium in a container.
- 17. The kit of claim 16 and wherein said container is a well and said plurality of organoids is contained in a plurality of wells of a culture plate wherein each well contains an organoid wherein the organoid is viable long term.
FIELD OF THE INVENTION
[0001] This application claims the benefit of U.S. Provisional Application No. 60/075,054 filed Feb. 18, 1998, and U.S. Provisional Application No. 60/086,370 filed May 22, 1998.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60075054 |
Feb 1998 |
US |
|
60086370 |
May 1998 |
US |