Claims
- 1. A method for screening compounds useful for the treatment of proliferative and differentiative disorders comprising contacting a compound with a cell or a cell extract expressing Skp2 and one or both of p27 and Cks1, and detecting a change in the activity of Skp2.
- 2. The method of claim 1 wherein the change in the activity of Skp2 is detected by detecting a change in the interaction of Skp2 with either p27 or Cks1.
- 3. The method of claim 1 wherein the change in the activity of Skp2 is detected by detecting a change in the ubiquitination of p27 or degradation of p27 or Cks 1.
- 4. A method for screening compounds useful for the treatment of proliferative and differentiative disorders comprising adding a compound in a purified system containing Skp2 and one or both of p27 and Cks1, and detecting a change in the activity of Skp2.
- 5. The method of claim 4 wherein the change in the activity of Skp2 is detected by detecting a change in the interaction of Skp2 with either p27 or Cks1.
- 6. The method of claim 4 wherein the change in the activity of Skp2 is detected by detecting a change in the ubiquitination of p27 or degradation of p27 or Cks1.
- 7. A method for screening compounds usefull for the treatment of proliferative and differentiative disorders comprising adding a compound in a purified system containing Skp2 and one or both of a polypeptide corresponding to the carboxy terminus of the human p27 chain having the sequence NAGSVEWTPKKPGLRRRQT with or without a phosphothreonine at position 187 and Cks1, and detecting a change in the activity of Skp2.
- 8. The method of claim 7 wherein the change in the activity of Skp2 is detected by detecting a change in the interaction of Skp2 with either the polypeptide or Cks1.
- 9. The method of claim 7 wherein the change in the activity of Skp2 is detected by detecting a change in the ubiquitination of the polypeptide or degradation of the polypeptide or Cks1.
Parent Case Info
[0001] This application claims priority under 35 U.S.C. § 119(e) to U.S. Application No. 60/260,179, filed Jan. 5, 2001, the contents of which are incorporated herein by reference in their entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60260179 |
Jan 2001 |
US |