Patent Grant
7608456
This application is the U.S. National Phase under 35 U.S.C. § 371 of International Application No. PCT/JP2005/006986, filed Apr. 5, 2005, which in turn claims the benefit of Japanese Application No. 2004-112975, filed Apr. 7, 2004, the disclosures of which Applications are incorporated by reference herein in their entirety.
The present invention relates to a microplate processing apparatus capable of performing given processes for microplates, and to a method of conveying microplates in the microplate processing apparatus.
In fields such as drug screening and biotechnology, biochemical reactions of substances and the like are tested. In the testing, a microplate is used as a container for containing drug solution and test substances with which cultivation and biochemical reactions are performed. The testing, usually performed systematically targeted for a large number of samples, processes a plurality of microplates in one testing for dispensing operation, componential analysis, and others.
Under the circumstances, as disclosed in Japanese Patent Unexamined Publication No. H11-223636, such a structure is known that is equipped with a feeding device for stocking a plurality of microplates; and a plate conveying mechanism for conveying microplates extracted from the feeding device, as a dedicated processing apparatus for performing the above-mentioned processes automatically. In this conventional example, microplates are individually contained in a storage rack provided with a plurality of plate placing positions; when feeding, microplates are extracted one by one by means of a plate conveying mechanism of a ceiling-travel robot type, and are transferred to the dispensing stage; and microplates having undergone the dispensing operation are returned to the storage rack.
A well for containing liquid, provided in a microplate, is open at its top surface. Therefore, when storing a microplate with liquid injected into its well, having undergone the dispensing operation, a lidded microplate may be used for isolating the injected liquid from the outside atmosphere, depending on a purpose of testing. In such a case, the lid needs to be attached and detached when performing processes such as dispensing operation for a microplate, and thus a microplate processing apparatus requires a function of attaching and detaching a microplate lid.
In the above-mentioned conventional example, for instance, the apparatus is equipped with a lid removing unit therein, and microplates individually move to the lid removing unit by the plate conveying mechanism every time before and after a dispensing operation is performed.
However, in the above-mentioned conventional example, only one set of lid removing unit is arranged at a fixed position. Consequently, a large number of microplates requiring being processed continuously make it difficult to attach and detach lids efficiently, which causes the entire processing to be inefficient.
A microplate processing apparatus according to the present invention has the following makeup.
A microplate processing apparatus that removes a microplate lid to perform a given process for the microplate, and attaches the lid after the process is completed, the apparatus equipped with a microplate conveying mechanism with a conveyor belt, for conveying the microplate in an order corresponding to a lid removing position, an intermediate position, and a lid attaching position, from upstream on a conveying path; a processing unit for performing a given process for the microplate at the intermediate position; a lid removing mechanism for removing the microplate lid at the lid removing position; a lid placing mechanism for placing the lid removed by the lid removing mechanism, on a downstream position from the microplate to which this lid was attached, on the conveying path of the microplate conveying mechanism; a lid attaching mechanism that picks up the lid conveyed from the lid removing position to the lid attaching position by the microplate conveying mechanism, by the microplate conveying mechanism, and attaches this lid to the microplate conveyed to the lid attaching position by the microplate conveying mechanism; a lid conveying processor for conveying the lid placed on this microplate conveying mechanism by the lid placing mechanism, from the lid removing position to the lid attaching position, by controlling the microplate conveying mechanism; and a microplate conveying processor that conveys the microplate with its lid removed by the lid removing mechanism, to the intermediate position, and conveys the microplate having undergone a given process by the processing unit, to the lid attaching position.
A method of conveying a microplate according to the present invention has the following steps.
A method of conveying a microplate in a microplate processing apparatus equipped with a microplate conveying mechanism with a conveyor belt, for conveying the microplate in an order corresponding to a lid removing position, an intermediate position, and a lid attaching position, from upstream on a conveying path, the method including a lid removing position conveying step for conveying the microplate with its lid attached, to the lid removing position, by the microplate conveying mechanism; a lid removing step for removing the microplate lid at the lid removing position; a lid placing step for placing the lid removed by the lid removing mechanism, on a downstream position from the microplate to which this lid was attached, on the conveying path of the microplate conveying mechanism; a lid conveying step for conveying the lid placed on this microplate conveying mechanism, from the lid removing position to the attaching position, by the microplate conveying mechanism; a lid picking up step for picking up the lid conveyed to the lid attaching position, from the microplate conveying mechanism, and for holding the lid; an intermediate position conveying step for conveying the microplate with its lid removed, by the microplate conveying mechanism; a lid attaching position conveying step for conveying the microplate having undergone a given process, to the lid attaching position, at the intermediate position; a lid attaching step for attaching the lid held in the lid picking up step, to the microplate conveyed to the lid attaching position; and a carrying out step for carrying out the microplate with its lid attached, from the lid attaching position, by the microplate conveying mechanism.
According to the present invention, the lid removed by the lid removing mechanism is placed on a downstream position from the microplate to which this lid was attached, on the conveying path of the microplate conveying mechanism, and conveyed to the lid attaching position in advance of the microplate. As a result that this lid is attached to the microplate conveyed to the lid attaching position after a given process is completed, attaching and detaching the lid to and from the microplate are performed efficiently.
Hereinafter, a description will be made for an embodiment of the present invention, using drawings.
First, a description will be made for the entire structure of microplate processing apparatus 1, referring to
Microplate processing apparatus 1, in biochemical testing and analysis such as in drug screening, has functions for removing the microplate lid to perform a given process for the microplate, and for attaching the lid after the process is completed. As shown in
Microplates 10, test objects, are contained in first plate feeding container 41 and second plate feeding container 42. Microplates 10 fed one by one from first plate feeding container 41 and second plate feeding container 42, are conveyed downstream (to the right in FIG. 1) by means of first microplate conveying mechanism 31 and second microplate conveying mechanism 32, both with conveyor belts.
Then, a dispensing operation is performed for microplates 10 by dispensing head 8, on the conveying paths of first microplate conveying mechanism 31 and second microplate conveying mechanism 32. Microplates 10 having undergone the dispensing operation are conveyed downstream by first microplate conveying mechanism 31 and second microplate conveying mechanism 32, and collected to first plate collecting container 51 and second plate collecting container 52.
Microplate 10, a test object, is to be stocked with lid 11 attached to prevent foreign matter from being immixed. Lid 11 is attached and detached when dispensing operation is performed by dispensing head 8. Accordingly, microplate processing apparatus 1 is provided with lid removing mechanism 6 and lid attaching mechanism 9 for removing and attaching lid 11, upstream and downstream of dispensing head 8, respectively. Lid removing mechanism 6 and lid attaching mechanism 9 move up and down first lid holding head 6b and second lid holding head 9b, both capable of sucking and holding lid 11, by means of first lid holding head up/down actuator 6a and second lid holding head up/down actuator 9a, respectively.
Next, a description is made for the structures of first microplate conveying mechanism 31 and second microplate conveying mechanism 32, and their positional segments on the conveying path, referring to
As shown in
The top surface of belt 13 born by bearing plate 3b for travel is a conveying path on which microplate 10 is placed and conveyed. The traveling direction of belt 13, namely the conveying direction, and the start/stop timing of conveying are arbitrarily controllable as required. The conveying path of microplate conveying mechanism 3 is segmented into the positions with their sizes in which microplate 10 can stay one each from upstream: first position [P1], second position [P2], third position [P3], fourth position [P4], and fifth position [P5].
First position [P1] is a receiving position for receiving microplate 10 fed from plate feeding containers 41 and 42 located upstream. The second position [P2] is a lid removing position for removing lid 11 from microplate 10 by lid removing mechanism 6. Meanwhile, fourth position [P4] is a lid attaching position for attaching lid 11 once removed at second position [P2], to microplate 10. Finally, third position [P3], located intermediately between second position [P2] and fourth position [P4], is an intermediate position for dispensing microplate 10 by dispensing head 8.
Therefore, in the above-mentioned makeup, dispensing head 8 is a processing unit for performing a given process for microplate 10 having been conveyed by microplate conveying mechanism 3, at the intermediate position. Lid removing mechanism 6 removes lid 11 of microplate 10 having been conveyed by microplate conveying mechanism 3, at second position [P2] (lid removing position). Further, lid attaching mechanism 9, to be hereinafter described, picks up lid 11 having been conveyed to fourth position [P4] in advance of microplate 10, after removed from microplate 10, and then attaches lid 11 to microplate 10 having been conveyed to fourth position [P4] later.
The respective positions are provided with sensors for detecting the presence of microplate 10 at each position: first sensor S1, second sensor S2, third sensor S3, fourth sensor S4, and fifth sensor S5. Meanwhile, first position [P1], second position [P2], third position [P3], and fourth position [P4] are provided with first stopper 151, second stopper 152, third stopper 153, and fourth stopper 154, respectively, for stopping microplate 10 at each position.
In other words, microplate conveying mechanism 3 is equipped with a plurality of stoppers for stopping microplate 10 at second position [P2], third position [P3], and fourth position [P4] (lid removing position, intermediate position, and lid attaching position, respectively).
Next, a description will be made for the makeup of the control system, referring to
Control unit 20 further controls first lid holding head up/down actuator 6a and second lid holding head up/down actuator 9a, for the attaching and detaching operation of lid 11 performed during the plate conveying operation. Control unit 20 controls the moving operation of dispensing head 8 when dispensing microplate 10, by controlling dispensing head drive mechanism 7. Control unit 20 controls the feeding and collecting operations of microplate 10, by controlling first plate feeding container 41, second plate feeding container 42, first plate collecting container 51, and second plate collecting container 52.
Next, a description is made for the processing functions of lid conveying processor 21, microplate conveying processor 22, and lid placing operation processor 23, shown in
First, a description is made for lid placing operation processor 23.
In the process of a plate by microplate processing apparatus 1, after lid 11 is removed at second position [P2] by lid removing mechanism 6, from microplate 10 that has been fed, with lid 11 attached, microplate 10 is conveyed to third position [P3], and a given process is performed for this microplate 10. Then, lid 11 is to be attached to microplate 10 conveyed to fourth position [P4] after the process is completed, by means of lid attaching mechanism 9. Here, the correspondence between microplate 10 and lid 11 is not in random order, but lid 11 that was attached when fed is to be always attached to its original microplate 10, in order to prevent foreign matter from being immixed into liquid contained in microplate 10.
Accordingly, in microplate processing apparatus 1 according to this embodiment, lid 11 removed at second position [P2] is conveyed to fourth position [P4] in advance of microplate 10, and is preliminarily held in lid attaching mechanism 9. Then, the lid 11 is to be attached to microplate 10, when microplate 10 after the process is completed at third position [P3], is conveyed to fourth position [P4].
In order to implement these lid attaching and detaching operations by the same microplate conveying mechanism 3, as described later, microplate conveying mechanism 3 is controlled by lid placing operation processor 23 so that the conveying direction of microplate 10 with lid 11 removed is reversed, to be conveyed upstream. This is to cause an evacuating operation that evacuates relevant microplate 10 from second position [P2] (lid removing position), and a lid placing operation that places lid 11 on second position [P2] where microplate 10 has been evacuated, by controlling lid removing mechanism 6.
In other words, an operational process is performed by a lid placing mechanism to be hereinafter described, the process that places lid 11 removed by lid removing mechanism 6, on a downstream position from microplate 10 to which this lid 11 was attached, on the conveying path of microplate conveying mechanism 3. In this way, after removed lid 11 is positioned downstream of the body of microplate 10, microplate 10 and lid 11 are conveyed by microplate conveying mechanism 3. As a result, microplate 10 fed together with lid 11 attached can be separately conveyed, that is, microplate 10 to third position [P3], and lid 11 to fourth position [P4].
Therefore, in this embodiment, lid placing operation processor 23 is the aforementioned lid placing mechanism. Lid attaching mechanism 9 picks up lid 11 conveyed from second position [P2] to fourth position [P4] by microplate conveying mechanism 3, from microplate conveying mechanism 3, and then attaches this lid 11 to microplate 10 conveyed to fourth position [P4] by microplate conveying mechanism 3.
In the above-mentioned lid attaching and detaching operations, lid 11 and microplate 10 need to be conveyed to a specified position at a specified timing. What perform this conveying process are lid conveying processor 21 and microplate conveying processor 22. Lid conveying processor 21 conveys lid 11 placed on microplate conveying mechanism 3 by the aforementioned lid placing mechanism, from second position [P2] to fourth position [P4], by controlling microplate conveying mechanism 3. Meanwhile, microplate conveying processor 22 conveys microplate 10 with lid 11 removed, to third position [P3] (intermediate position), by controlling microplate conveying mechanism 3, and conveys microplate 10 having undergone a dispensing process by dispensing head 8 at third position [P3], to fourth position [P4].
Microplate processing apparatus 1 is composed as mentioned above. A description will be hereinafter made for a method of conveying microplates in microplate processing apparatus 1, referring to
When starting the process in microplate processing apparatus 1, microplate 10 fed from first plate feeding container 41 or second plate feeding container 42, is placed on first position [P1] with lid 11 attached, as shown in
Then, microplate 10 is stopped at second position [P2] by second stopper 152 that is preliminarily located at the elevated position. In other words, microplate 10 with lid 11 attached is conveyed to second position [P2] (lid removing position), by microplate conveying mechanism 3 (lid removing position conveying step). Here, when a given standby time elapses after second sensor S2 detects microplate 10, first lid holding head 6b moves down, to remove lid 11 from microplate 10, as shown in
After then, as shown in
As a result that the microplate evacuating step and lid placing step shown here are executed, the lid placing step is executed that places lid 11 removed in the lid removing step, on a downstream position from microplate 10 with this lid 11 having been attached, on the conveying path of microplate conveying mechanism 3. In other words, the lid placing step includes the microplate evacuating step and lid placing step.
After then, microplate 10 and lid 11 are conveyed. More specifically, as shown in
At this moment, lid 11 reaches fourth position [P4], to be stopped by fourth stopper 154. When a given standby time elapses after fourth sensor S4 detects that this lid 11 has reached, third stopper 153 is elevated. This causes, as shown in
In other words, the following steps are executed here: a lid conveying step for conveying lid 11 placed on this microplate conveying mechanism 3, from second position [P2] to fourth position [P4], by microplate conveying mechanism 3; a lid picking up step for picking up lid 11 conveyed to fourth position [P4], from microplate conveying mechanism 3, to be held; and an intermediate position conveying step for conveying microplate 10 with lid 11 removed, to third position [P3] (intermediate position), by microplate conveying mechanism 3.
Then in third position [P3], when a given standby time elapses after microplate 10 is detected by third sensor S3, a dispensing process targeted for microplate 10 by dispensing head 8 is started. At this moment, first position [P1] is fed with new microplate 10 with lid 11 attached, and second stopper 152 is elevated. After that, when a given time elapses after microplate 10 is detected by second sensor S2, travel of belt 13 is stopped, and the completion of the dispensing process at third position [P3] is waited. At this moment, lid 11 is not removed from microplate 10, but the lid-attached state is held, at second position [P2].
After that, when the dispensing process by dispensing head 8 is completed at third position [P3], microplate 10 having undergone a dispensing operation is conveyed to fourth position [P4], as shown in
This causes lid 11 removed from microplate 10 in the lid removing step, to be attached to the original microplate 10 to which relevant lid 11 was attached, in the lid attaching step. After that, when second lid holding head 9b is elevated, fourth stopper 154 is released to convey microplate 10 to fifth position [P5], as shown in
Subsequently, when a given time elapses after fifth sensor S5 detects that microplate 10 has passed through, it is judged that carrying out of microplate 10 that has turned into a lid-attached state after the dispensing process, from microplate conveying mechanism 3, has been completed, as shown in
As mentioned above, as a result that lid 11 removed by lid removing mechanism 6 is placed on a downstream position from the original microplate 10, on the conveying path of microplate conveying mechanism 3, to be conveyed to the lid attaching position in advance of this microplate 10, lid 11 can be attached to microplate 10 having undergone a process. This makeup allows lid 11 to be attached and detached efficiently in a microplate processing apparatus that performs processes while continuously conveying a large number of microplates 10 by a single microplate conveying mechanism.
A microplate processing apparatus according to the present invention is effective in that the lid of a microplate is efficiently attached to and detached from the microplate, for continuous processes targeted for a microplate requiring a lid to be attached.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2004-112975 | Apr 2004 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP2005/006986 | 4/5/2005 | WO | 00 | 10/31/2005 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2005/098453 | 10/20/2005 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 3844896 | Sharpe | Oct 1974 | A |
| 5985214 | Stylli et al. | Nov 1999 | A |
| 6360792 | Ganz et al. | Mar 2002 | B1 |
| 6979425 | Ganz et al. | Dec 2005 | B1 |
| 20020119077 | Shumate et al. | Aug 2002 | A1 |
| 20030044991 | Haslam et al. | Mar 2003 | A1 |
| Number | Date | Country |
|---|---|---|
| 11-223636 | Aug 1999 | JP |
| 2002-340912 | Nov 2001 | JP |
| 2004-85521 | Mar 2004 | JP |
| Number | Date | Country | |
|---|---|---|---|
| 20060210431 A1 | Sep 2006 | US |
