The invention relates to the field of microarrays and more particularly to an apparatus and method for separating, identifying and quantitating chemical moieties using arrays.
Polynucleotide arrays (such as DNA or RNA arrays) are known and are used, for example, as diagnostic or screening tools. Such arrays include regions of usually different sequence polynucleotides arranged in a predetermined configuration on a substrate. These regions (sometimes referenced as “features”) are positioned at respective locations (“addresses”) on the substrate. In use, the arrays, when exposed to a sample, will exhibit an observed binding or hybridization pattern. This binding pattern can be detected upon interrogating the array. For example, all polynucleotide targets (for example, DNA) in the sample can be labeled with a suitable label (such as a fluorescent dye), and the fluorescence pattern on the array accurately observed following exposure to the sample. Assuming that the different sequence polynucleotides were correctly deposited in accordance with the predetermined configuration, then the observed binding pattern will be indicative of the presence and/or concentration of one or more polynucleotide components of the sample.
Biopolymer arrays can be fabricated by depositing previously obtained biopolymers (such as from synthesis or natural sources) onto a substrate, or by in situ synthesis methods. Methods of depositing obtained biopolymers include dispensing droplets to a substrate from dispensers such as pin or capillaries (such as described in U.S. Pat. No. 5,807,522) or such as pulse-jets (such as a piezoelectric inkjet head, as described in PCT publications WO 95/25116 and WO 98/41531, and elsewhere). For in situ fabrication methods, multiple different reagent droplets are deposited from drop dispensers at a given target location in order to form the final feature (hence a probe of the feature is synthesized on the array substrate). The in situ fabrication methods include those described in U.S. Pat. No. 5,449,754 for synthesizing peptide arrays, and described in WO 98/41531 and the references cited therein for polynucleotides. The in situ method for fabricating a polynucleotide array typically follows, at each of the multiple different addresses at which features are to be formed, the same conventional iterative sequence used in forming polynucleotides from nucleoside reagents on a support by methods of known chemistry. This iterative sequence is as follows: (a) coupling a selected nucleoside through a phosphite linkage to a functionalized support in the first iteration, or a nucleoside bound to the substrate (i.e. the nucleoside-modified substrate) in subsequent iterations; (b) optionally, but preferably, blocking unreacted hydroxyl groups on the substrate bound nucleoside; (c) oxidizing the phosphite linkage of step (a) to form a phosphate linkage; and (d) removing the protecting group (“deprotection”) from the now substrate bound nucleoside coupled in step (a), to generate a reactive site for the next cycle of these steps. The functionalized support (in the first cycle) or deprotected coupled nucleoside (in subsequent cycles) provides a substrate bound moiety with a linking group for forming the phosphite linkage with a next nucleoside to be coupled in step (a). Final deprotection of nucleoside bases can be accomplished using alkaline conditions such as ammonium hydroxide, in a known manner.
The foregoing chemistry of the synthesis of polynucleotides is described in detail, for example, in Caruthers, Science 230: 281-285, 1985; Itakura et al., Ann. Rev. Biochem. 53: 323-356; Hunkapillar et al., Nature 310: 105-110, 1984; and in “Synthesis of Oligonucleotide Derivatives in Design and Targeted Reaction of Oligonucleotide Derivatives”, CRC Press, Boca Raton, Fla., pages 100 et seq., U.S. Pat. No. 4,458,066, U.S. Pat. No. 4,500,707, U.S. Pat. No. 5,153,319, U.S. Pat. No. 5,869,643, EP 0294196, and elsewhere.
As discussed above, there are a number of techniques for constructing microarrays. In addition, microarrays may be used to identify and quantitate different types of RNA, DNA or protein molecules in a sample. A microarray comprises a number of surface bound molecules that may be arranged in defined locations. For instance, a sample containing an unknown target is often labeled with a fluorescent dye, applied to the array and allowed to react or hybridize to a probe over a period of time. The array is then washed to remove unbound or inappropriately bound sample and scanned for fluorescent signal. The detected signal at each location is correlated to the probe identity.
In the above example, the array provides a few major functions. The first function is that it acts as a separation device that organizes molecules from the sample into known locations and allows the remainder to be discarded. Second, it is a platform to analyze how many sample molecules were detected at each location. The two functions are independent and each confers its own requirements on the assay design.
The separation function requires that the known probe molecule be attached to the surface in a known or defined location. The pattern of features can be in the form of a grid or a linear arrangement. The detection of these hybridizations is due largely to the use of fluorescent dyes coupled to target molecules. Labeling is typically performed during a sample preparation process that can add significant time to the assay completion. Secondly, the use of labels increases costs, and can potentially cross react with other molecules or probes. Therefore, there is a need for an array system, apparatus or technique that eliminates the need for using labels. There is also a need for such apparatus or method to provide a high level of specificity and reproducibility for identifying and separating small sample volumes or quantities.
Microarrays also suffer from the limitation that they can require multiple runs and may require extensive time to employ in an analysis. In addition, they may be limited by hybridization parameters such as requiring a 20 mer or smaller to obtain complete hybridizations. Each of these requirements, therefore, influences the effectiveness of microarrays as clinical or diagnostic devices. Therefore, there is a need for analytical devices to be able to separate and identify targets at high speeds.
In contrast, nanopore technologies are now being developed to sequence genomes and nucleic acids, or proteins at high speeds. These techniques attempt to sequence the nucleic acid or protein when it passes through a defined nanopore or structure. Manipulating matter at the nanometre (nm) scale is important for many electronic, chemical and biological advances (See Li et al., “Ion beam sculpting at nanometer length scales”, Nature, 412: 166-169, 2001).
It has been demonstrated that a voltage gradient can drive single-stranded biopolymers through a transmembrane channel, or nanopore (See Kasianowicz et al., “Characterization of individual polynucleotide molecules using a membrane channel”, Proc. Natl. Acad. Sci. USA, 93: 13770-13773, 1996). During the translocation process, the extended biopolymer molecule will block a substantial portion of the otherwise open nanopore channel. This blockage leads to a decrease in the ionic current flow of the buffer solution through the nanopore during the biopolymer translocation. The passage of a single biopolymer can be monitored by recording the translocation duration and the blockage current, yielding plots with predictable stochastic sensing patterns. From the uniformly controlled translocation conditions, the lengths of the individual biopolymers can be determined from the translocation time. Furthermore, the differing physical and chemical properties of the individual bases of the biopolymer strand can in principle generate a measurable and reproducible modulation of the blockage current that allows an identification of the specific base sequence of the translocating biopolymer. These initially proposed systems suffer from a number of problems. For instance, some of the proposed systems require self-assembly of pore forming proteins on membranes (i.e. ahemolysin). Reproducibility of membranes an systems has been quite problematic. Secondly, commercial products require robustness not present in sensitive systems that require fluctuations of ionic currents for measurements. For these reasons, recent research has focused more on solid-state pore techniques that have an ability for high reproducibility and ease of fabrication.
Another method for detecting a biopolymer translocating through a nanopore has been proposed. This technique is based upon quantum mechanical tunneling currents through the portion of the translocating strand as it passes between a pair of electrodes. Measuring the magnitude of the tunneling current would be an electronic method of detecting the presence of a translocating biopolymer, and if the conditions were adequately controlled and the measurements sufficiently sensitive, the sequence of constituent bases could also be determined. One of the primary motivations for this approach is that typical tunneling currents in scanning tunneling microscopes are on the order of 1-10 nanoamps, which is two to three orders of magnitude larger than the ionic currents observed during polymer translocation of 2 nanometer nanopores, as described above (See Kasianowicz et al., “Characterization of individual polynucleotide molecules using a membrane channel”, Proc. Natl. Acad. Sci. USA, 93: 13770-13773, 1996).
The problem with such techniques is that they generally require nucleic acids that are free of other contaminants such as ribonucleic acid (RNA), proteins, or other molecules. Therefore, there is a need to purify or remove contaminants before the molecule to be sequenced reaches the nanopore. Otherwise, the extraneous material or contaminants will interfere with the quality of the overall results. Secondly, these high speed sequencing technologies require a way to easily and efficiently input the biomolecules to be sequenced. Of the present separation and sequencing devices to date none provide stability and ease of fabrication. These problems and others are addressed by the present invention.
The invention provides an apparatus for identifying a chemical moiety from a sample solution. The system or apparatus comprises a substrate or housing having a channel with at least one microarray for capturing a chemical moiety from a sample solution, and a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the microarray.
The invention also provides a method for separating and identifying a chemical moiety. The method comprises contacting a solution comprising a target molecule to a probe positioned in a micro fluidic channel, binding the target molecule to the probe to separate the target molecule from the solution, releasing the target molecule off of the probe, and identifying or sequencing the target molecule released from the probe. Sequencing or identification may be by way of a solid state nanopore system. In particular the system may be ramped to perform resonant tunneling on the target of interest.
Embodiments of the invention will now be described with reference to the drawings, in which:
Before describing the invention in detail, it must be noted that, as used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “an array” includes more than one “array”. Reference to an “electrode” or “substrate” includes more than one “electrode” or “substrate”. In describing and claiming the present invention, the following terminology will be used in accordance with the definitions set out below.
The term “adjacent” or “adjacent to” refers to a component or element that is near, next to or adjoining. For instance, an array may be adjacent to a nanopore system.
A “biopolymer” is a polymer of one or more types of repeating units. Biopolymers are typically found in biological systems (although they may be made synthetically) and particularly include peptides or polynucleotides, as well as such compounds composed of or containing amino acid analogs or non-amino acid groups, or nucleotide analogs or non-nucleotide groups. This includes polynucleotides in which the conventional backbone has been replaced with a non-naturally occurring or synthetic backbone, and nucleic acids (or synthetic or naturally occurring analogs) in which one or more of the conventional bases has been replaced with a group (natural or synthetic) capable of participating in Watson-Crick type hydrogen bonding interactions. Polynucleotides include single or multiple stranded configurations, where one or more of the strands may or may not be completely aligned with another. A “nucleotide” refers to a sub-unit of a nucleic acid and has a phosphate group, a 5 carbon sugar and a nitrogen containing base, as well as functional analogs (whether synthetic or naturally occurring) of such sub-units which in the polymer form (as a polynucleotide) can hybridize with naturally occurring polynucleotides in a sequence specific manner analogous to that of two naturally occurring polynucleotides. For example, a “biopolymer” includes DNA (including cDNA), RNA, oligonucleotides, and PNA and other polynucleotides as described in U.S. Pat. No. 5,948,902 and references cited therein (all of which are incorporated herein by reference), regardless of the source. An “oligonucleotide” generally refers to a nucleotide multimer of about 10 to 100 nucleotides in length, while a “polynucleotide” includes a nucleotide multimer having any number of nucleotides. A “biomonomer” references a single unit, which can be linked with the same or other biomonomers to form a biopolymer (for example, a single amino acid or nucleotide with two linking groups one or both of which may have removable protecting groups). A “peptide” is used to refer to an amino acid multimer of any length (for example, more than 10, 10 to 100, or more amino acid units). A biomonomer fluid or biopolymer fluid references a liquid containing either a biomonomer or biopolymer, respectively (typically in solution).
The term “in” refers to being “within” and/or a portion that may also be exterior to. For instance, a biopolymer “in” a nanopore may mean that the whole biopolymer is within the opening of the nanopore or only a small portion of the biopolymer is located near the nanopore with a substantial portion protruding exterior to the nanopore.
The term “nanopore” refers to any pore or hole between at least a pair of electrodes or a hole in a solid substrate. Nanopores can range in size from around 1 nm to around 300 nm. Most effective nanopores have been roughly 2-20 nms.
The term “portion” or “portion of a biopolymer” refers to a part, subunit, monomeric unit, portion of a monomeric unit, atom, portion of an atom, cluster of atoms, charge or charged unit.
The term “ramping potential” or “bias potential” refers to having the ability to establish a variety of different voltages over time. In certain cases this may be referred to as “scanning a voltage gradient” or altering a voltage gradient per unit of time. The ramping potential is produced by the “potential means”.
The term “resonant” or “resonant tunneling” refers to an effect where the relative energy levels between the current carriers in the electrodes are relatively similar to the energy levels of the proximal biopolymer segment. This provides for increased conductivity.
A “set” or “sub-set” of any item (for example, a set of features) may contain one or more than one of the item (for example, a set of clamp members may contain one or more such members). An “array”, unless a contrary intention appears, includes any one, two or three dimensional arrangement of addressable regions bearing a particular chemical moiety or moieties (for example, biopolymers such as polynucleotide sequences) associated with that region. An array is “addressable” in that it has multiple regions of different moieties (for example, different polynucleotide sequences) such that a region (a “feature” or “spot” of the array) at a particular predetermined location (an “address”) on the array will detect a particular target or class of targets (although a feature may incidentally detect non-targets of that feature). Array features are typically, but need not be, separated by intervening spaces. In the case of an array, the “target” will be referenced as a moiety in a mobile phase (typically fluid), to be detected by probes (“target probes”) which are bound to the substrate at the various regions. However, either of the “target” or “target probes” may be the one that is to be evaluated by the other (thus, either one could be an unknown mixture of polynucleotides to be evaluated by binding with the other). An “array layout” refers collectively to one or more characteristics of the features, such as feature positioning, one or more feature dimensions, and some indication of a moiety at a given location. “Hybridizing” and “binding”, with respect to polynucleotides, are used interchangeably. When one item is indicated as being “remote” from another, this is referenced that the two items are at least in different buildings, and may be at least one mile, ten miles, or at least one hundred miles apart.
The term “substrate” or “substrate surface” are synonymous and refer to the material an electrode may be attached, comprise or be embedded in.
The term “symmetric” or “symmetrized” refers to similar tunneling barriers adjacent to both electrodes.
The term “translocation” or to “translocate” refers to movement from one side to another, or movement in a defined direction.
The term “tunneling” refers to the ability of an electron to move from a first position in space to a second position in space through a region that would be energetically excluded without quantum mechanical tunneling.
All patents and other cited references are incorporated into this application by reference.
The first substrate 115 may comprise a number of different materials well know in the art. For instance, the first substrate 115 may comprise a material selected from the group consisting of metals, plastics, polycarbonate materials, rubber, silica or silicone based materials, and composite materials. The first substrate 115 may comprise flexible or non-flexible materials. As mentioned the first substrate 115 may comprise one or more channels 118. The channel 118 may comprise a micro fluidic channel 118 having one or more probes 121 (See
The sample may comprise one or more targets 103 that are transported through the channel 118. Transport may be accomplished through osmotic pressure, fluidic pressure, Brownian motion, diffusion, osmotic gradient, electro-osmotic gradient, gravity, capillary action, active or passive transport, electrophoresis, pressure, suction or creation of a vacuum or artificial vacuum or other physical or mechanical forces that are well know in the art. The technique is not important. However, functionally it is important that the technique efficiently regulates and allows the targets 103 to bind to the probes 121 that are attached or positioned in the channel 118 of the first substrate 115.
The array 112 may comprise a microarray or similar type device. As discussed, the array 112 may be constructed on the interior wall of channel 118 (See
Referring now to
The array 112 may comprise a biopolymer or in particular a nucleic acid or nucleotide sequence. Other biopolymers know in the art may be employed such as proteins, peptides, amino acids, nucleotides, nucleosides, nucleic acids, RNA, DNA, single stranded RNA, single stranded DNA, double stranded DNA or RNA etc., may be employed with the present invention. The target 103 or probe 121 sequence may be known or unknown. The biopolymers may be arranged in any of a number of orders and/or orientations on the array 112. This allows for the capture and release of biopolymers in a defined order or sequence.
The nanopore system 120 is positioned downstream from the array 112 (See
Typical nanopore systems 120 used with the present invention may comprise and are not limited to devices disclosed and discussed in U.S. patent application Ser. No. 10/353,675 filed on Jan. 27, 2003 entitled “Apparatus and Method for Biopolymer Identification During Translocation Through a Nanopore” and Ser. No. 10/462,216 filed on Jun. 12, 2003 entitled “Nanopore with Resonant Tunneling Electrodes”. These disclosures are herein incorporated by reference in their entirety. Other solid-state nanopore devices known in the art may also be employed with the present invention.
Referring now to
The biopolymer 5 may comprise a variety of shapes, sizes and materials. The shape or size of the molecule is not important, but it must be capable of translocation through the nanopore 3. For instance, both single stranded and double stranded RNA and DNA may be used as a biopolymer 5. In addition, the biopolymer 5 may contain groups or functional groups that are charged. Furthermore, metals or materials may be added, doped or intercalated within the biopolymer 5 to provide a net dipole, a charge or allow for conductivity through the biomolecule. The material of the biopolymer must allow for electron tunneling between electrodes.
The first electrode 7 may comprise a variety of electrically conductive materials. Such materials include electrically conductive metals and alloys of tin, copper, zinc, iron, magnesium, cobalt, nickel, and vanadium. Other materials well known in the art that provide for electrical conduction may also be employed. When the first electrode 7 is deposited on or comprises a portion of the solid substrate 8, it may be positioned in any location relative to the second electrode 9. It must be positioned in such a manner that a potential can be established between the first electrode 7 and the second electrode 9. In addition, the bipolymer 5 must be positioned sufficiently close so that a portion of it may be identified or sequenced. In other words, the first electrode 7, the second electrode 9, and the nanopore 3 must be spaced and positioned in such a way that the bipolymer 5 may be identified or sequenced. This should not be interpreted to mean that the embodiment shown in
The second electrode 9 may comprise the same or similar materials as described above for the first electrode 7. As discussed above, its shape, size and positioning may be altered relative to the first electrode 7 and the nanopore 3.
The optional substrate 8 may comprise a variety of materials known in the art for designing substrates and nanopores. The substrate 8 may or may not be a solid material. For instance, the substrate 8 may comprise a mesh, wire, or other material that a nanopore may be constructed. Such materials may comprise silicon, silica, solid-state material such as Si3N4, carbon based materials, plastics, metals, or other materials known in the art for etching or fabricating semiconductor or electrically conducting materials. The solid substrate 8 may comprise various shapes and sizes. However, it must be large enough and of sufficient width to be capable of forming the nanopore 3 through it.
The nanopore 3 may be positioned anywhere on/through the optional substrate 8. As described above, the nanopore 3 may also be established by the spacing between the first electrode 7 and the second electrode 9 (in a planar or non planar arrangement). When the substrate 8 is employed, it should be positioned adjacent to the first electrode 7 and the second electrode 9. The nanopore may range in size from 1 nm to as large as 300 nms. In most cases, effective nanopores for identifying and sequencing biopolymers would be in the range of around 2-20 nm. These size nanopores are just large enough to allow for tranlocation of a biopolymer. The nanopore 3 may be established using any methods well known in the art. For instance, the nanopore 3, may be sculpted in the substrate 8, using argon ion beam sputtering, etching, photolithography, or other methods and techniques well known in the art.
The voltage source 11 may be positioned anywhere relative to the substrate 8, the nanopore 3, the first electrode 7 and the second electrode 9. The voltage source 11 should be capable of ramping to establish a voltage gradient between the first electrode 7 and the second electrode 9. A variety of voltage sources 11 may be employed with the present invention. A number of these voltage sources are known in the art. The voltage source 11 has the ability to ramp to establish a voltage gradient between the first electrode 7 and the second electrode 9. This is an important aspect of the present invention and for this reason is discussed in more detail below.
An optional means for signal detection may be employed to detect the signal produced from the bipolymer and voltage source 11. This means for signal detection may be any structure, component or apparatus that is well known in the art and that may be electrically connected to one or more components of the present invention.
Referring now to
Referring now to
Referring now to
As seen in
One particular point along the biopolymer 334 represented as bead 336 is shown in a favorable position for resonant tunneling to occur. A voltage source, not shown, applies a time-varying potential difference between electrodes 310 and 314, via a circuit, not shown, similar to that used for embodiment 100, and the resulting time-varying current is measured by a current measuring means, not shown, similar to that shown in embodiment 100, in order to characterize that portion of biopolymer molecule 334 which happens to be in the favorable position for resonant tunneling to occur. For more information regarding the method of fabrication please refer to the reference Ser. No. 10/462,216 filed on Jun. 12, 2003 entitled “Nanopore with Resonant Tunneling Electrodes”.
While a significant benefit of the apparatus is to avoid the use of labels, it may be advantageous in certain instances to combine detection techniques. A labeled sample allows for fluorescent detection in-situ. A follow up elution or mass spectrometry measurement may provide more detailed information or confirmation of the measurements. Alternatively, it may be desirable to elute the target and analyze by gel eletrophoresis. This secondary and more expensive approach may be of interest for a reference laboratory or a central research facility.
Having described the apparatus of the invention, a description of the method of assembling or making the array hybridization apparatus is now in order.
Referring now to
The RNA sample comprising unlabeled RNA and known control DNA targets is fragmented to lengths of approximately 200 mer using methods known in the art. Using a pipette, the channel is filled or nearly filled with the target. The chip is placed into an instrument that removably seals valve-controlled fluidic lines to each of the openings in the chip. In addition, the instrument controls the temperature of the chip.
The input valve 135 and exit valve 137 are adjusted so that each end of the chip is connected to a source of variable and controllable pressure that may be alternated to be above and below standard pressure as needed (See
After the hybridization period is complete, the instrument's valve at the inlet of the glass chip switches from the pressure pulses to the first wash fluid. The valve switches from pressure to the waste container. The first wash fluid is pumped through the channel driving the sample to the waste container and washing the array surface to remove unbound or non-specially bound sample. The wash fluid is generally not recirculated, although it may be. Next, the input valve 135 is switched to a second wash fluid that is added by way of wash buffer inlet port 138 as required by the assay. The wash fluid may be pumped through the channel for several minutes. During the wash protocol, the chip's temperature is generally returned to room temperature.
At the conclusion of the wash protocol, the input valve 135 is switched to the elution buffer. Elution buffer is pumped into the channel by way of the elution buffer inlet port 140 until the wash buffer is removed to waste (See
The exit valve 137 switches to the electrospray tip 130 and the electrospray mass spectrometer is then activated. The elution buffer is driven through the electrospray tip 130 into the mass spectrometer. The amount of target 103 eluted at each time point is quantitated. Since the flow rate is known, the signal at each time point can be correlated to each feature on the array for target identification. The control targets are used to establish the starting and ending points of the array as well as validate the timing along the array.
In summary, the method of the present invention operates for separating and detecting a chemical moiety such as a biopolymer. The steps of the method comprise contacting a sample comprising a target molecule to a probe positioned in a channel of a substrate, capturing the target molecule by contacting it with a probe, releasing the target molecule from the probe in a defined order and detecting the target molecule released from the probe in the defined order.
Clearly, minor changes may be made in the form and construction of the invention without departing from the scope of the invention defined by the appended claims. It is not, however, desired to confine the invention to the exact form herein shown and described, but it is desired to include all such as properly come within the scope claimed.