Microbicidal composition

Information

  • Patent Application
  • 20090023688
  • Publication Number
    20090023688
  • Date Filed
    July 08, 2008
    16 years ago
  • Date Published
    January 22, 2009
    15 years ago
Abstract
Synergistic microbicidal compositions containing N-methyl-1,2-benzisothiazolin-3-one.
Description

This patent application claims the benefit of the earlier filed European Patent application serial number 07290901.3 filed on Jul. 18, 2007 under 37 CFR 1.55(a).


This invention relates to a synergistic combination of selected microbicides having greater activity than would be observed for the individual microbicides.


In some cases, commercial microbicides cannot provide effective control of microorganisms, even at high use concentrations, due to weak activity against certain types of microorganisms, e.g., those resistant to some microbicides, or due to aggressive environmental conditions. Combinations of different microbicides are sometimes used to provide overall control of microorganisms in a particular end use environment. For example, U.S. Pat. App. Pub. No. 2007/0078118 discloses synergistic combinations of N-methyl-1,2-benzisothiazolin-3-one (MBIT) with other biocides. However, there is a need for additional combinations of microbicides having enhanced activity against various strains of microorganisms to provide effective control of the microorganisms. Moreover, there is a need for combinations containing lower levels of individual microbicides for environmental and economic benefit. The problem addressed by this invention is to provide such additional combinations of microbicides.


STATEMENT OF THE INVENTION

The present invention is directed to a microbicidal composition comprising: (a) N-methyl-1,2-benzisothiazolin-3-one; and (b) at least one microbicide selected from the group consisting of hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine; 4,4-dimethyloxazolidine; sodium hydroxymethylglycinate; myristamidopropyl PG-dimoonium chloride phosphate; octanoic acid; 2-ethylhexyl glycerin; glyceryl monocaprylate; glyceryl monocaprate; 7α-ethyldihydro-1H,3H,5H-oxazolo (3,4-C) oxazole; and 2-(thiocyanomethylthio)benzothiazole.







DETAILED DESCRIPTION OF THE INVENTION

As used herein, the following terms have the designated definitions, unless the context clearly indicates otherwise. “MBIT” is N-methyl-1,2-benzisothiazolin-3-one. The term “microbicide” refers to a compound capable of killing, inhibiting the growth of or controlling the growth of microorganisms at a locus; microbicides include bactericides, fungicides and algaecides. The term “microorganism” includes, for example, fungi (such as yeast and mold), bacteria and algae. The term “locus” refers to an industrial system or product subject to contamination by microorganisms. The following abbreviations are used throughout the specification: ppm=parts per million by weight (weight/weight), mL=milliliter, ATCC=American Type Culture Collection, MBC=minimum biocidal concentration, and MIC=minimum inhibitory concentration. Unless otherwise specified, temperatures are in degrees centigrade (° C.), and references to percentages (%) are by weight. Amounts of organic microbicides are given on an active ingredient basis in ppm (w/w).


The compositions of the present invention unexpectedly have been found to provide enhanced microbicidal efficacy at a combined active ingredient level lower than that of the individual microbicides. Additional microbicides beyond those listed in the claims may be present in the composition.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine. Preferably, a weight ratio of hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0014 to 1:150, more preferably from 1:0.012 to 1:150.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and 4,4-dimethyloxazolidine. Preferably, a weight ratio of 4,4-dimethyloxazolidine to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0011 to 1:3.5.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and sodium hydroxymethylglycinate. Preferably, a weight ratio of sodium hydroxymethylglycinate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0005 to 1:2.3, more preferably from 1:0.14 to 1:2.3, more preferably from 1:0.29 to 1:2.3.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and myristamidopropyl PG-dimoonium chloride phosphate. Preferably, a weight ratio of myristamidopropyl PG-dimoonium chloride phosphate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0011 to 1:150.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and octanoic acid. Preferably, a weight ratio of octanoic acid to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0046 to 1:0.68, more preferably from 1:0.029 to 1:0.68.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and 2-ethylhexyl glycerin. Preferably, a weight ratio of 2-ethylhexyl glycerin to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.017 to 1:2.3.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and glyceryl monocaprylate. Preferably, a weight ratio of glyceryl monocaprylate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0004 to 1:75, more preferably from 1:0.012 to 1:75.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and glyceryl monocaprate. Preferably, a weight ratio of glyceryl monocaprate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0031 to 1:8.8, more preferably from 1:0.017 to 1:8.8.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and 7α-ethyldihydro-1H,3H,5H-oxazolo (3,4-C) oxazole. Preferably, a weight ratio of 7α-ethyldihydro-1H,3H,5H-oxazolo (3,4-C) oxazole to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0003 to 1:7.1.


In one embodiment of the invention, the antimicrobial composition comprises N-methyl-1,2-benzisothiazolin-3-one and 2-(thiocyanomethylthio)benzothiazole. Preferably, a weight ratio of 2-(thiocyanomethylthio)benzothiazole to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.063 to 1:143.


The microbicides in the composition of this invention may be used “as is” or may first be formulated with a solvent or a solid carrier. Suitable solvents include, for example, water; glycols, such as ethylene glycol, propylene glycol, diethylene glycol, dipropylene glycol, polyethylene glycol, and polypropylene glycol; glycol ethers; alcohols, such as methanol, ethanol, propanol, phenethyl alcohol and phenoxypropanol; ketones, such as acetone and methyl ethyl ketone; esters, such as ethyl acetate, butyl acetate, triacetyl citrate, and glycerol triacetate; carbonates, such as propylene carbonate and dimethyl carbonate; and mixtures thereof. It is preferred that the solvent is selected from water, glycols, glycol ethers, esters and mixtures thereof Suitable solid carriers include, for example, cyclodextrin, silicas, diatomaceous earth, waxes, cellulosic materials, alkali and alkaline earth (e.g., sodium, magnesium, potassium) metal salts (e.g., chloride, nitrate, bromide, sulfate) and charcoal.


When a microbicide component is formulated in a solvent, the formulation may optionally contain surfactants. When such formulations contain surfactants, they are generally in the form of emulsive concentrates, emulsions, microemulsive concentrates, or microemulsions. Emulsive concentrates form emulsions upon the addition of a sufficient amount of water. Microemulsive concentrates form microemulsions upon the addition of a sufficient amount of water. Such emulsive and microemulsive concentrates are generally well known in the art; it is preferred that such formulations are free of surfactants. U.S. Pat. No. 5,444,078 may be consulted for further general and specific details on the preparation of various microemulsions and microemulsive concentrates.


A microbicide component also can be formulated in the form of a dispersion. The solvent component of the dispersion can be an organic solvent or water, preferably water. Such dispersions can contain adjuvants, for example, co-solvents, thickeners, anti-freeze agents, dispersants, fillers, pigments, surfactants, biodispersants, sulfosuccinates, terpenes, furanones, polycations, stabilizers, scale inhibitors and anti-corrosion additives.


When both microbicides are each first formulated with a solvent, the solvent used for the first microbicide may be the same as or different from the solvent used to formulate the other commercial microbicide, although water is preferred for most industrial biocide applications. It is preferred that the two solvents are miscible.


Those skilled in the art will recognize that the microbicide components of the present invention may be added to a locus sequentially, simultaneously, or may be combined before being added to the locus. It is preferred that the first microbicide and the second microbicide component be added to a locus simultaneously or sequentially. When the microbicides are added simultaneously or sequentially, each individual component may contain adjuvants, such as, for example, solvent, thickeners, anti-freeze agents, colorants, sequestrants (such as ethylenediamine-tetraacetic acid, ethylenediaminedisuccinic acid, iminodisuccinic acid and salts thereof), dispersants, surfactants, biodispersants, sulfosuccinates, terpenes, furanones, polycations, stabilizers, scale inhibitors and anti-corrosion additives.


The microbicidal compositions of the present invention can be used to inhibit the growth of microorganisms or higher forms of aquatic life (such as protozoans, invertebrates, bryozoans, dinoflagellates, crustaceans, mollusks, etc.) by introducing a microbicidally effective amount of the compositions onto, into, or at a locus subject to microbial attack. Suitable loci include, for example: industrial process water; electrocoat deposition systems,; cooling towers; air washers; gas scrubbers; mineral slurries; wastewater treatment; ornamental fountains; reverse osmosis filtration; ultrafiltration; ballast water; evaporative condensers; heat exchangers; pulp and paper processing fluids and additives; starch; plastics; emulsions; dispersions; paints; latices; coatings, such as varnishes; construction products, such as mastics, caulks, and sealants; construction adhesives, such as ceramic adhesives, carpet backing adhesives, and laminating adhesives; industrial or consumer adhesives; photographic chemicals; printing fluids; household products, such as bathroom and kitchen cleaners and sanitary wipes; cosmetics; toiletries; shampoos; soaps; detergents; industrial cleaners; floor polishes; laundry rinse water; metalworking fluids; conveyor lubricants; hydraulic fluids; leather and leather products; textiles; textile products; wood and wood products, such as plywood, chipboard, wallboard, flakeboard, laminated beams, oriented strandboard, hardboard, and particleboard; petroleum processing fluids; fuel; oilfield fluids, such as injection water, fracture fluids, and drilling muds; agriculture adjuvant preservation; surfactant preservation; medical devices; diagnostic reagent preservation; food preservation, such as plastic or paper food wrap; food, beverage, and industrial process pasteurizers; toilet bowls; recreational water; pools; and spas.


Preferably, the microbicidal compositions of the present invention are used to inhibit the growth of microorganisms at a locus selected from one or more of mineral slurries, pulp and paper processing fluids and additives, starch, emulsions, dispersions, paints, latices, coatings, construction adhesives, such as ceramic adhesives, carpet backing adhesives, photographic chemicals, printing fluids, household products such as bathroom and kitchen cleaners and sanitary wipes, cosmetics, toiletries, shampoos, soaps, detergents, industrial cleaners, floor polishes, laundry rinse water, metal working fluids, textile products, wood and wood products, agriculture adjuvant preservation, surfactant preservation, diagnostic reagent preservation, food preservation, and food, beverage, and industrial process pasteurizers.


The specific amount of the composition of this invention necessary to inhibit or control the growth of microorganisms and higher aquatic life forms in a locus depends upon the particular locus to be protected. Typically, the amount of the composition of the present invention to control the growth of microorganisms in a locus is sufficient if it provides from 0.1 to 1,000 ppm of the isothiazoline ingredient of the composition in the locus. It is preferred that the isothiazolone ingredients of the composition be present in the locus in an amount of at least 0.5 ppm, more preferably at least 4 ppm and most preferably at least 10 ppm. It is preferred that the isothiazolone ingredients of the composition be present in the locus in an amount of no more than 1000 ppm, more preferably no more than 500 ppm, and most preferably no more than 200 ppm.


EXAMPLES
Materials and Methods

The synergism of the combination of the present invention was demonstrated by testing a wide range of concentrations and ratios of the compounds.


One measure of synergism is the industrially accepted method described by Kull, F. C.; Eisman, P. C.; Sylwestrowicz, H. D. and Mayer, R. L., in Applied Microbiology 9:538-541 (1961), using the ratio determined by the formula:






Q
a
/Q
A
+Q
b
/Q
B=Synergy Index (“SI”)


wherein:

    • QA=concentration of compound A (first component) in ppm, acting alone, which produced an end point (MIC of Compound A).
    • Qa=concentration of compound A in ppm, in the mixture, which produced an end point.
    • QB=concentration of compound B (second component) in ppm, acting alone, which produced an end point (MIC of Compound B).
    • Qb=concentration of compound B in ppm, in the mixture, which produced an end point.


When the sum of Qa/QA and Qb/QB is greater than one, antagonism is indicated. When the sum is equal to one, additivity is indicated, and when less than one, synergism is demonstrated. The lower the SI, the greater the synergy shown by that particular mixture. The minimum inhibitory concentration (MIC) of a microbicide is the lowest concentration tested under a specific set of conditions that prevents the growth of added microorganisms.


Synergy tests were conducted using standard microtiter plate assays with media designed for optimal growth of the test microorganism. Minimal salt medium supplemented with 0.2% glucose and 0.1% yeast extract (M9GY medium) was used for testing bacteria; Potato Dextrose Broth (PDB medium) was used for testing yeast and mold. In this method, a wide range of combinations of microbicides was tested by conducting high resolution MIC assays in the presence of various concentrations of MBIT. High resolution MICs were determined by adding varying amounts of microbicide to one column of a microtitre plate and doing subsequent ten-fold dilutions using an automated liquid handling system to obtain a series of endpoints ranging from 2 ppm to 10,000 ppm active ingredient.


The synergy of the combinations of the present invention was determined against several microorganisms, as described in the Tables below. The bacteria were used at a concentration of about 5×106 bacteria per mL and the yeast and mold at 5×105 fungi per mL. These microorganisms are representative of natural contaminants in many consumer and industrial applications. The plates were visually evaluated for microbial growth (turbidity) to determine the MIC after various incubation times at 25° C. (yeast and mold) or 30° C. (bacteria).


The test results for demonstration of synergy of the MBIT combinations of the present invention are shown below in Tables 1 through 10. In each test, Second Component (B) was MBIT and the First Component (A) was the other commercial microbicide. Each table shows the specific combinations of MBIT and the other component; results against the microorganisms tested with incubation times; the end-point activity in ppm measured by the MIC for MBIT alone (QB), for the other component alone (QA), for MBIT in the mixture (Qb) and for the other component in the mixture (Qa); the calculated SI value; and the range of synergistic ratios for each combination tested (other component/MBIT or A/B).














TABLE 1






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
425





ATCC # 16404


37.5






110
18.8
0.76
1:0.1709




200
18.8
0.97
1:0.0940




300
18.8
1.21
1:0.0627




300
9.4
0.96
1:0.0313




425
9.4
1.25
1:0.0221




300
4.7
0.83
1:0.0157




425
4.7
1.13
1:0.0111




300
2.4
0.77
1:0.0080




425
2.4
1.06
1:0.0056




300
1.2
0.74
1:0.0040




425
1.2
1.03
1:0.0028



 7 days
650








150






42.5
75
0.57
1:1.7647




65
75
0.60
1:1.1538




110
75
0.67
1:0.6818




200
75
0.81
1:0.3750




300
75
0.96
1:0.2500




400
75
1.12
1:0.1875




425
18.8
0.78
1:0.0442




525
18.8
0.93
1:0.0358




650
9.4
1.06
1:0.0145




525
4.7
0.84
1:0.0090




650
4.7
1.03
1:0.0072




525
2.4
0.82
1:0.0046




650
2.4
1.02
1:0.0037




650
1.2
1.01
1:0.0018



C. albicans

48 hrs
875





ATCC # 10231


30






87.5
15
0.60
1:0.1714




110
15
0.63
1:0.1364




200
15
0.73
1:0.0750




300
15
0.84
1:0.0500




425
15
0.99
1:0.0353




110
7.5
0.38
1:0.0682




200
7.5
0.48
1:0.0375




300
7.5
0.59
1:0.0250




425
7.5
0.74
1:0.0176




525
7.5
0.85
1:0.0143




650
7.5
0.99
1:0.0115




425
3.75
0.61
1:0.0088




525
3.75
0.73
1:0.0071




650
3.75
0.87
1:0.0058




875
3.75
1.13
1:0.0043




650
1.86
0.80
1:0.0029




875
1.86
1.06
1:0.0021




650
0.94
0.77
1:0.0014




875
0.94
1.03
1:0.0011



72 hrs
875








30






87.5
15
0.60
1:0.1714




110
15
0.63
1:0.1364




200
15
0.73
1:0.0750




300
15
0.84
1:0.0500




425
15
0.99
1:0.0353




425
7.5
0.74
1.0.0176




525
7.5
0.85
1:0.0143




650
7.5
0.99
1:0.0115




875
1.86
1.06
1:0.0021




875
0.94
1.03
1:0.0011



Ps. aeruginosa

24 hrs
55





ATCC # 9027


75






32.5
37.5
1.09
1:1.1538




43.75
18.75
1.05
1:0.4286




43.75
9.4
0.92
1:0.2149




43.75
4.6
0.86
1:0.1051



48 hrs
55








125






26.25
75
1.08
1:2.8571




32.5
37.5
0.89
1:1.1538




43.75
37.5
1.10
1:0.8571




55
4.6
1.04
1:0.0836



S. aureus

24 hrs
55





ATCC # 6538


15






15
7.5
0.77
1:0.5000




32.5
3.8
0.84
1:0.1169




43.75
3.8
1.05
1:0.0869




32.5
1.8
0.71
1:0.0554




43.75
1.8
0.92
1:0.0411




55
1.8
1.12
1:0.0327




55
0.94
1.06
1:0.0171



48 hrs
55








30






0.1
15
0.50
1:150.0000




1.5
15
0.53
1:10.0000




2.625
15
0.55
1:5.7143




4.375
15
0.58
1:3.4286




5.5
15
0.60
1:2.7273




10
15
0.68
1:1.5000




15
15
0.77
1:1.0000




21.25
15
0.89
1:0.7059




26.25
15
0.98
1:0.5714




32.5
15
1.09
1:0.4615




21.25
7.5
0.64
1:0.3529




26.25
7.5
0.73
1:0.2857




32.5
7.5
0.84
1:0.2308




43.75
7.5
1.05
1:0.1714




32.5
3.8
0.72
1:0.1169




43.75
3.8
0.92
1:0.0869




55
3.8
1.13
1:0.0691




32.5
1.8
0.65
1:0.0554




55
1.8
1.06
1:0.0327




43.75
0.94
0.83
1:0.0215




55
0.94
1.03
1:0.0171





Ca: ppm AI of BIOBAN GK(hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 2






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
525





ATCC # 16404


37.5






200
18.8
0.88
1:0.0940




300
18.8
1.07
1:0.0627




300
9.4
0.82
1:0.0313




425
9.4
1.06
1:0.0221




425
4.7
0.93
1:0.0111




525
4.7
1.13
1:0.0090




425
2.4
0.87
1:0.0056




525
2.4
1.06
1:0.0046




425
1.2
0.84
1:0.0028




525
1.2
1.03
1:0.0023



 7 days
875








150






525
75
1.1 
1:0.1429




650
37.5
0.99
1:0.0577




875
9.4
1.06
1:0.0107




650
4.7
0.77
1:0.0072




875
4.7
1.03
1:0.0054




650
2.4
0.76
1:0.0037




875
2.4
1.02
1:0.0027




875
1.2
1.01
1:0.0014



C. albicans

48 hrs
1100





ATCC # 10231


30






425
15
0.89
1:0.0353




525
15
0.98
1:0.0286




650
15
1.09
1:0.0231




650
7.5
0.84
1:0.0115




875
7.5
1.05
1:0.0086




875
3.75
0.92
1:0.0043




1100
3.75
1.13
1:0.0034




875
1.86
0.86
1:0.0021




1100
1.86
1.06
1:0.0017




875
0.94
0.83
1:0.0011




1100
0.94
1.03
1:0.0009



72 hrs
1100








30






425
15
0.89
1:0.0353




525
15
0.98
1:0.0286




650
15
1.09
1:0.0231




875
7.5
1.05
1:0.0086




875
3.75
0.92
1:0.0043




1100
3.75
1.13
1:0.0034




875
1.86
0.86
1:0.0021




1100
1.86
1.06
1:0.0017




875
0.94
0.83
1:0.0011




1100
0.94
1.03
1:0.0009



Ps. aeruginosa

24 hrs
100





ATCC # 9027


75






55
37.5
1.05
1:0.6818




55
9.4
0.68
1:0.1709




100
9.4
1.13
1:0.0940




55
4.6
0.61
1:0.0836




100
4.6
1.06
1:0.0460



48 hrs
100








125






21.25
75
0.81
1:3.5294




26.25
75
0.86
1:2.8571




32.5
75
0.93
1:2.3077




43.75
75
1.04
1:1.7143




55
37.5
0.85
1:0.6818




100
9.4
1.08
1:0.0940




100
4.6
1.04
1:0.0460



S. aureus

24 hrs
55





ATCC # 6538


30






21.25
15
0.89
1:0.7059




26.25
15
0.98
1:0.5714




32.5
15
1.09
1:0.4615




26.25
7.5
0.73
1:0.2857




32.5
7.5
0.84
1:0.2308




43.75
7.5
1.05
1:0.1714




43.75
3.8
0.92
1:0.0869




55
3.8
1.13
1:0.0691




55
1.8
1.06
1:0.0327




55
0.94
1.03
1:0.0171



48 hrs
55








30






21.25
15
0.89
1:0.7059




26.25
15
0.98
1:0.5714




32.5
15
1.09
1:0.4615




26.25
7.5
0.73
1:0.2857




32.5
7.5
0.84
1:0.2308




43.75
7.5
1.05
1:0.1714




43.75
3.8
0.92
1:0.0869




55
3.8
1.13
1:0.0691




55
1.8
1.06
1:0.0327




55
0.94
1.03
1:0.0171





Ca: ppm AI of BIOBAN 1135 (4,4-dimethyloxazolidine)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 3






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
875





ATCC # 16404


75






425
18.8
0.74
1:0.0442




525
18.8
0.85
1:0.0358




650
18.8
0.99
1:0.0289




425
9.4
0.61
1:0.0221




525
9.4
0.73
1:0.0179




650
9.4
0.87
1:0.0145




875
9.4
1.13
1:0.0107




650
4.7
0.81
1:0.0072




875
4.7
1.06
1:0.0054




525
2.4
0.63
1:0.0046




650
2.4
0.77
1:0.0037




875
2.4
1.03
1:0.0027




650
1.2
0.76
1:0.0018




875
1.2
1.02
1:0.0014



 7 days
1100








150






110
75
0.60
1:0.6818




200
75
0.68
1:0.3750




300
75
0.77
1:0.2500




425
75
0.89
1:0.1765




525
75
0.98
1:0.1429




650
75
1.09
1:0.1154




425
37.5
0.64
1:0.0882




525
37.5
0.73
1:0.0714




650
37.5
0.84
1:0.0577




875
37.5
1.05
1:0.0429




525
18.8
0.6 
1:0.0358




650
18.8
0.72
1:0.0289




875
18.8
0.92
1:0.0215




1100
18.8
1.13
1:0.0171




525
9.4
0.54
1:0.0179




650
9.4
0.65
1:0.0145




875
9.4
0.86
1:0.0107




1100
9.4
1.06
1:0.0085




650
4.7
0.62
1:0.0072




875
4.7
0.83
1:0.0054




1100
4.7
1.03
1:0.0043




650
2.4
0.61
1:0.0037




875
2.4
0.81
1:0.0027




1100
2.4
1.02
1:0.0022




650
1.2
0.60
1:0.0018




875
1.2
0.80
1:0.0014




1100
1.2
1.01
1:0.0011



C. albicans

48 hrs
3000





ATCC # 10231


30






525
15
0.68
1:0.0286




650
15
0.72
1:0.0231




875
15
0.79
1:0.0171




1100
15
0.87
1:0.0136




2000
15
1.17
1:0.0075




525
7.5
0.43
1:0.0143




650
7.5
0.47
1:0.0115




875
7.5
0.54
1:0.0086




1100
7.5
0.62
1:0.0068




2000
7.5
0.92
1:0.0038




3000
7.5
1.25
1:0.0025




875
3.75
0.42
1:0.0043




1100
3.75
0.49
1:0.0034




2000
3.75
0.79
1:0.0019




3000
3.75
1.13
1:0.0013




2000
1.86
0.73
1:0.0009




3000
1.86
1.06
1:0.0006




2000
0.94
0.70
1:0.0005




3000
0.94
1.03
1:0.0003



72 hrs
3000








30






525
15
0.68
1:0.0286




650
15
0.72
1:0.0231




875
15
0.79
1:0.0171




1100
15
0.87
1:0.0136




2000
15
1.17
1:0.0075




3000
15
1.5 
1:0.0050




525
7.5
0.43
1:0.0143




650
7.5
0.47
1:0.0115




875
7.5
0.54
1:0.0086




1100
7.5
0.62
1:0.0068




2000
7.5
0.92
1:0.0038




3000
7.5
1.25
1:0.0025




875
3.75
0.42
1:0.0043




1100
3.75
0.49
1:0.0034




2000
3.75
0.79
1:0.0019




3000
3.75
1.13
1:0.0013




2000
1.86
0.73
1:0.0009




3000
1.86
1.06
1:0.0006




2000
0.94
0.70
1:0.0005




3000
0.94
1.03
1:0.0003



Ps. aeruginosa

24 hrs
87.5





ATCC # 9027


75






65
9.4
0.87
1:0.1446




87.5
9.4
1.13
1:0.1074



48 hrs
110








125






87.5
9.4
0.87
1:0.1074




110
9.4
1.08
1:0.0855




110
4.6
1.04
1:0.0418



S. aureus

24 hrs
110





ATCC # 6538


15






30
7.5
0.77
1:0.2500




42.5
7.5
89   
1:0.1765




52.5
7.5
98   
1:0.1429




65
7.5
1.09
1:0.1154




52.5
3.8
0.73
1:0.0724




65
3.8
0.84
1:0.0585




87.5
3.8
1.05
1:0.0434




110
0.94
1.06
1:0.0085



48 hrs
110








30






6.5
15
0.56
1:2.3077




8.75
15
0.58
1:1.7143




11
15
0.6 
1:1.3636




20
15
0.68
1:0.7500




30
15
0.77
1:0.5000




42.5
15
0.89
1:0.3529




52.5
15
0.98
1:0.2857




65
15
1.09
1:0.2308




42.5
7.5
0.64
1:0.1765




52.5
7.5
0.73
1:0.1429




65
7.5
0.84
1:0.1154




87.5
7.5
1.05
1:0.0857




65
3.8
0.72
1:0.0585




110
1.8
1.06
1:0.0164




110
0.94
1.03
1:0.0085





Ca: ppm AI of SUTTOCIDE A (sodium hydroxymethylglycinate)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 4






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
1100





ATCC # 16404


37.5






425
18.8
0.89
1:0.0442




525
18.8
0.98
1:0.0358




650
18.8
1.09
1:0.0289




525
9.4
0.73
1:0.0179




650
9.4
0.84
1:0.0145




875
9.4
1.05
1:0.0107




1100
2.4
1.06
1:0.0022




1100
1.2
1.03
1:0.0011



 7 days
1100








150






2
75
0.50
1:37.5000




20
75
0.52
1:3.7500




200
75
0.68
1:0.3750




525
75
0.98
1:0.1429




650
75
1.09
1:0.1154




20
37.5
0.27
1:1.8750




52.5
37.5
0.3 
1:0.7143




87.5
37.5
0.33
1:0.4286




200
37.5
0.43
1:0.1875




425
37.5
0.64
1:0.0882




650
37.5
0.84
1:0.0577




875
37.5
1.05
1:0.0429




650
18.8
0.72
1:0.0289




875
18.8
0.92
1:0.0215




1100
18.8
1.13
1:0.0171




650
9.4
0.65
1:0.0145




875
9.4
0.86
1:0.0107




1100
9.4
1.06
1:0.0085




1100
4.7
1.03
1:0.0043




1100
2.4
1.02
1:0.0022




1100
1.2
1.01
1:0.0011



C. albicans

48 hrs
1100





ATCC # 10231


30






525
15
0.98
1:0.0286




650
15
1.09
1:0.0231




525
7.5
0.73
1:0.0143




650
7.5
0.84
1:0.0115




875
7.5
1.05
1:0.0086




650
3.75
0.72
1:0.0058




875
3.75
0.92
1:0.0043




1100
3.75
1.13
1:0.0034




875
1.86
0.86
1:0.0021




1100
1.86
1.06
1:0.0017




875
0.94
0.83
1:0.0011




1100
0.94
1.03
1:0.0009



72 hrs
1100








60






2
30
0.50
1:15.0000




20
30
0.52
1:1.5000




110
30
0.60
1:0.2727




200
30
0.68
1:0.1500




300
30
0.77
1:0.1000




425
30
0.89
1:0.0706




525
30
0.98
1:0.0571




650
30
1.09
1:0.0462




525
15
0.73
1:0.0286




650
15
0.84
1:0.0231




525
7.5
0.6 
1:0.0143




650
7.5
0.72
1:0.0115




875
7.5
0.92
1:0.0086




650
3.75
0.65
1:0.0058




875
3.75
0.86
1:0.0043




1100
3.75
1.06
1:0.0034




875
1.86
0.83
1:0.0021




1100
1.86
1.03
1:0.0017




875
0.94
0.81
1:0.0011




1100
0.94
1.02
1:0.0009



Ps. aeruginosa

24 hrs
425





ATCC # 9027


75






425
4.6
1.06
1:0.0108



48 hrs
525








125






525
4.6
1.04
1:0.0088



S. aureus

24 hrs
10





ATCC # 6538


15






2.625
7.5
0.76
1:2.8571




3.25
7.5
0.83
1:2.3077




4.375
7.5
0.94
1:1.7143




5.5
7.5
1.05
1:1.3636




4.375
3.8
0.69
1:0.8686




5.5
3.8
0.80
1:0.6909




10
3.8
1.25
1:0.3800




4.375
1.8
0.56
1:0.4114




5.5
1.8
0.67
1:0.3273




10
1.8
1.12
1:0.1800




10
0.94
1.06
1:0.0940



48 hrs
10








30






0.1
15
0.51
1:150.0000




0.5
15
0.55
1:30.0000




1
15
0.6 
1:15.0000




1.5
15
0.65
1:10.0000




2.125
15
0.71
1:7.0588




2.625
15
0.76
1:5.7143




3.25
15
0.83
1:4.6154




4.375
15
0.94
1:3.4286




5.5
15
1.05
1:2.7273




4.375
7.5
0.69
1:1.7143




5.5
7.5
0.80
1:1.3636




10
7.5
1.25
1:0.7500




4.375
3.8
0.56
1:0.8686




5.5
3.8
0.68
1:0.6909




10
3.8
1.13
1:0.3800




5.5
1.8
0.61
1:0.3273




10
1.8
1.06
1:0.1800




10
0.94
1.03
1:0.0940





Ca: ppm AI of Phospholipid PTM(myristamidopropyl PG-dimoonium chloride phosphate)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 5






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
650





ATCC # 16404


75






425
18.8
0.9 
1:0.0442




525
18.8
1.06
1:0.0358




525
9.4
0.93
1:0.0179




650
9.4
1.13
1:0.0145




525
4.7
0.87
1:0.0090




525
2.4
0.84
1:0.0046




650
2.4
1.03
1:0.0037




650
1.2
1.02
1:0.0018



 7 days
875








150






110
75
0.63
1:0.6818




200
75
0.73
1:0.3750




300
75
0.84
1:0.2500




425
75
0.99
1:0.1765




650
37.5
0.99
1:0.0577




425
18.8
0.61
1:0.0442




650
18.8
0.87
1:0.0289




875
18.8
1.13
1:0.0215




875
9.4
1.06
1:0.0107




875
4.7
1.03
1:0.0054




875
2.4
1.02
1:0.0027




875
1.2
1.01
1:0.0014



C. albicans

48 hrs
65





ATCC # 10231


30






42.5
7.5
0.9 
1:0.1765




52.5
7.5
1.06
1:0.1429




65
1.86
1.06
1:0.0286




65
0.94
1.03
1:0.0145



72 hrs
87.5








30






42.5
15
0.99
1:0.3529




42.5
7.5
0.74
1:0.1765




52.5
7.5
0.85
1:0.1429




65
7.5
0.99
1:0.1154




65
3.75
0.87
1:0.0577




87.5
3.75
1.13
1:0.0429




87.5
1.86
1.06
1:0.0213




87.5
0.94
1.03
1:0.0107



Ps. aeruginosa

48 hrs
>11000





ATCC # 9027


125






8750
75
1.4 
1:0.0086




11000
37.5
1.3 
1:0.0034




11000
18.75
1.15
1:0.0017




11000
9.4
1.08
1:0.0009




11000
4.6
1.04
1:0.0004



S. aureus

24 hrs
425





ATCC # 6538


15






425
0.94
1.06
1:0.0022



48 hrs
425








30






425
1.8
1.06
1:0.0042




425
0.94
1.03
1:0.0022





Ca: ppm AI of Caprylic acid (octanoic acid)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 6






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
875





ATCC # 16404


37.5






650
9.4
0.99
1:0.0145




650
4.7
0.87
1:0.0072




875
4.7
1.13
1:0.0054




875
2.4
1.06
1:0.0027




875
1.2
1.03
1:0.0014



 7 days
1100








150






87.5
75
0.58
1:0.8571




110
75
0.60
1:0.6818




200
75
0.68
1:0.3750




300
75
0.77
1:0.2500




425
75
0.89
1:0.1765




525
75
0.98
1:0.1429




650
75
1.09
1:0.1154




650
37.5
0.84
1:0.0577




1100
9.4
1.06
1:0.0085




1100
4.7
1.03
1:0.0043




1100
2.4
1.02
1:0.0022




1100
1.2
1.01
1:0.0011



C. albicans

48 hrs
2000





ATCC # 10231


30






6.5
15
0.50
1:2.3077




11
15
0.51
1:1.3636




110
15
0.56
1:0.1364




200
15
0.60
1:0.0750




300
15
0.65
1:0.0500




650
15
0.83
1:0.0231




875
15
0.94
1:0.0171




1100
15
1.05
1:0.0136




2000
1.86
1.06
1:0.0009




2000
0.94
1.03
1:0.0005



72 hrs
2000








30






425
15
0.71
1:0.0353




525
15
0.76
1:0.0286




650
15
0.83
1:0.0231




875
15
0.94
1:0.0171




1100
15
1.05
1:0.0136




2000
1.86
1.06
1:0.0009




2000
0.94
1.03
1:0.0005



Ps. aeruginosa

24 hrs
11000





ATCC # 9027


75






8750
37.5
1.3 
1:0.0043



48 hrs
11000








125






4250
75
0.99
1:0.0176



S. aureus

24 hrs
2000





ATCC # 6538


30






8.75
15
0.5 
1:1.7143




20
15
0.51
1:0.7500




42.5
15
0.52
1:0.3529




65
15
0.53
1:0.2308




110
15
0.56
1:0.1364




200
15
0.6 
1:0.0750




425
15
0.71
1:0.0353




525
15
0.76
1:0.0286




650
15
0.83
1:0.0231




875
15
0.94
1:0.0171




1100
15
1.05
1:0.0136




2000
1.8
1.06
1:0.0009




2000
0.94
1.03
1:0.0005



48 hrs
2000








30






8.75
15
0.50
1:1.7143




20
15
0.51
1:0.7500




42.5
15
0.52
1:0.3529




65
15
0.53
1:0.2308




110
15
0.56
1:0.1364




200
15
0.60
1:0.0750




425
15
0.71
1:0.0353




525
15
0.76
1:0.0286




650
15
0.83
1:0.0231




875
15
0.94
1:0.0171




1100
15
1.05
1:0.0136




2000
1.8
1.06
1:0.0009




2000
0.94
1.03
1:0.0005





Ca: ppm AI of SENSIVA SC 50(3[(2-ethylhexyl)oxy]1,2-propanediol) (2- ethylhexyl glycerin)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 7






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
4250





ATCC # 16404


75






2000
37.5
0.97
1:0.0188




3000
37.5
1.21
1:0.0125




2000
18.8
0.72
1:0.0094




3000
18.8
0.96
1:0.0063




4250
18.8
1.25
1:0.0044




2000
9.4
0.60
1:0.0047




3000
9.4
0.83
1:0.0031




4250
9.4
1.13
1:0.0022




2000
4.7
0.53
1:0.0024




3000
4.7
0.77
1:0.0016




4250
4.7
1.06
1:0.0011




2000
2.4
0.5 
1:0.0012




3000
2.4
0.74
1:0.0008




4250
2.4
1.03
1:0.0006




3000
1.2
0.72
1:0.0004




4250
1.2
1.02
1:0.0003



 7 days
4250








150






1100
75
0.76
1:0.0682




2000
75
0.97
1:0.0375




3000
75
1.21
1:0.0250




2000
37.5
0.72
1:0.0188




3000
37.5
0.96
1:0.0125




4250
37.5
1.25
1:0.0088




2000
18.8
0.60
1:0.0094




3000
18.8
0.83
1:0.0063




4250
18.8
1.13
1:0.0044




2000
9.4
0.53
1:0.0047




3000
9.4
0.77
1:0.0031




4250
9.4
1.06
1:0.0022




2000
4.7
0.50
1:0.0024




3000
4.7
0.74
1:0.0016




4250
4.7
1.03
1:0.0011




2000
2.4
0.49
1:0.0012




3000
2.4
0.72
1:0.0008




4250
2.4
1.02
1:0.0006



C. albicans

48 hrs
200





ATCC # 10231


30






0.2
15
0.50
1:75.0000




2
15
0.51
1:7.5000




20
15
0.60
1:0.7500




42.5
15
0.71
1:0.3529




65
15
0.83
1:0.2308




87.5
15
0.94
1:0.1714




110
15
1.05
1:0.1364




200
1.86
1.06
1:0.0093




200
0.94
1.03
1:0.0047



72 hrs
200








30






0.2
15
0.50
1:75.0000




2
15
0.51
1:7.5000




20
15
0.6 
1:0.7500




42.5
15
0.71
1:0.3529




65
15
0.83
1:0.2308




87.5
15
0.94
1:0.1714




110
15
1.05
1:0.1364




200
1.86
1.06
1:0.0093




200
0.94
1.03
1:0.0047



Ps. aeruginosa

48 hrs
>11000





ATCC # 9027


125






2000
75
0.78
1:0.0375




3000
75
0.87
1:0.0250




4250
75
0.99
1:0.0176



S. aureus

24 hrs
650





ATCC # 6538


30






0.2
15
0.50
1:75.0000




2
15
0.50
1:7.5000




6.5
15
0.51
1:2.3077




11
15
0.52
1:1.3636




20
15
0.53
1:0.7500




30
15
0.55
1:0.5000




42.5
15
0.57
1:0.3529




52.5
15
0.58
1:0.2857




65
15
0.60
1:0.2308




87.5
15
0.63
1:0.1714




110
15
0.67
1:0.1364




200
15
0.81
1:0.0750




300
15
0.96
1:0.0500




425
15
1.15
1:0.0353




425
7.5
0.9 
1:0.0176




525
7.5
1.06
1:0.0143



48 hrs
875








30






0.2
15
0.50
1:75.0000




2
15
0.50
1:7.5000




6.5
15
0.51
1:2.3077




11
15
0.51
1:1.3636




20
15
0.52
1:0.7500




30
15
0.53
1:0.5000




42.5
15
0.55
1:0.3529




52.5
15
0.56
1:0.2857




65
15
0.57
1:0.2308




87.5
15
0.60
1:0.1714




110
15
0.63
1:0.1364




200
15
0.73
1:0.0750




300
15
0.84
1:0.0500




425
15
0.99
1:0.0353




650
7.5
0.99
1:0.0115




875
1.8
1.06
1:0.0021




875
0.94
1.03
1:0.0011





Ca: ppm AI of CAPMUL MCM C8 (glyceryl monocaprylate)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 8






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
2000





ATCC # 16404


75






4.25
37.5
0.50
1:8.8235




6.5
37.5
0.50
1:5.7692




11
37.5
0.51
1:3.4091




30
37.5
0.52
1:1.2500




65
37.5
0.53
1:0.5769




110
37.5
0.56
1:0.3409




200
37.5
0.60
1:0.1875




300
37.5
0.65
1:0.1250




425
37.5
0.71
1:0.0882




525
37.5
0.76
1:0.0714




650
37.5
0.83
1:0.0577




875
37.5
0.94
1:0.0429




1100
37.5
1.05
1:0.0341




1100
18.8
0.8 
1:0.0171




2000
4.7
1.06
1:0.0024




2000
2.4
1.03
1:0.0012




2000
1.2
1.02
1:0.0006



 7 days
2000








150






525
75
0.76
1:0.1429




650
75
0.83
1:0.1154




875
75
0.94
1:0.0857




1100
75
1.05
1:0.0682




1100
37.5
0.8 
1:0.0341




1100
18.8
0.68
1:0.0171




2000
9.4
1.06
1:0.0047




2000
4.7
1.03
1:0.0024




2000
2.4
1.02
1:0.0012




2000
1.2
1.01
1:0.0006



C. albicans

48 hrs
425





ATCC # 10231


30






87.5
15
0.71
1:0.1714




110
15
0.76
1:0.1364




200
15
0.97
1:0.0750




300
15
1.21
1:0.0500




200
7.5
0.72
1:0.0375




300
7.5
0.96
1:0.0250




425
7.5
1.25
1:0.0176




200
3.75
0.60
1:0.0188




300
3.75
0.83
1:0.0125




425
3.75
1.13
1:0.0088




200
1.86
0.53
1:0.0093




300
1.86
0.77
1:0.0062




425
1.86
1.06
1:0.0044




200
0.94
0.50
1:0.0047




300
0.94
0.74
1:0.0031




425
0.94
1.03
1:0.0022



72 hrs
425








30






87.5
15
0.71
1:0.1714




110
15
0.76
1:0.1364




200
15
0.97
1:0.0750




300
15
1.21
1:0.0500




200
7.5
0.72
1:0.0375




300
7.5
0.96
1:0.0250




425
7.5
1.25
1:0.0176




200
3.75
0.60
1:0.0188




300
3.75
0.83
1:0.0125




425
3.75
1.13
1:0.0088




200
1.86
0.53
1:0.0093




300
1.86
0.77
1:0.0062




425
1.86
1.06
1:0.0044




200
0.94
0.50
1:0.0047




300
0.94
0.74
1:0.0031




425
0.94
1.03
1:0.0022



Ps. aeruginosa

24 hrs
11000





ATCC # 9027


75







37.5







18.75







9.4







4.6





48 hrs
11000








125







75







37.5







18.75







9.4







4.6





S. aureus

24 hrs
200





ATCC # 6538


30






5.25
15
0.53
1:2.8571




11
15
0.56
1:1.3636




20
15
0.60
1:0.7500




30
15
0.65
1:0.5000




42.5
15
0.71
1:0.3529




52.5
15
0.76
1:0.2857




65
15
0.83
1:0.2308




87.5
15
0.94
1:0.1714




110
15
1.05
1:0.1364



48 hrs
200








30






65
15
0.83
1:0.2308




87.5
15
0.94
1:0.1714




110
15
1.05
1:0.1364





Ca: ppm AI of CapmulMCM C10 (Mono/digyceride of capric acid (Glyceryl Monocaprate))


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 9






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
650





ATCC # 16404


37.5






200
18.8
0.81
1:0.0940




300
18.8
0.96
1:0.0627




425
18.8
1.16
1:0.0442




425
9.4
0.90
1:0.0221




525
9.4
1.06
1:0.0179




425
4.7
0.78
1:0.0111




525
4.7
0.93
1:0.0090




650
4.7
1.13
1:0.0072




425
2.4
0.72
1:0.0056




525
2.4
0.87
1:0.0046




650
2.4
1.06
1:0.0037




425
1.2
0.69
1:0.0028




525
1.2
0.84
1:0.0023




650
1.2
1.03
1:0.0018



 7 days
875








150






425
75
0.99
1:0.1765




650
37.5
0.99
1:0.0577




525
18.8
0.73
1:0.0358




650
18.8
0.87
1:0.0289




875
18.8
1.13
1:0.0215




875
9.4
1.06
1:0.0107




875
4.7
1.03
1:0.0054




875
2.5
1.02
1:0.0029




875
1.2
1.01
1:0.0014



C. albicans

48 hrs
3000





ATCC # 10231


30






875
15
0.79
1:0.0171




1100
15
0.87
1:0.0136




2000
15
1.17
1:0.0075




875
7.5
0.54
1:0.0086




1100
7.5
0.62
1:0.0068




2000
7.5
0.92
1:0.0038




3000
7.5
1.25
1:0.0025




2000
3.75
0.79
1:0.0019




3000
3.75
1.13
1:0.0013




2000
1.86
0.73
1:0.0009




3000
1.86
1.06
1:0.0006




3000
0.94
1.03
1:0.0003



72 hrs
4250








30






875
15
0.71
1:0.0171




1100
15
0.76
1:0.0136




2000
15
0.97
1:0.0075




3000
15
1.21
1:0.0050




875
7.5
0.46
1:0.0086




1100
7.5
0.51
1:0.0068




2000
7.5
0.72
1:0.0038




3000
7.5
0.96
1:0.0025




4250
7.5
1.25
1:0.0018




2000
3.75
0.6 
1:0.0019




3000
3.75
0.83
1:0.0013




4250
3.75
1.13
1:0.0009




2000
1.86
0.53
1:0.0009




3000
0.94
0.74
1:0.0003




4250
0.94
1.03
1:0.0002



Ps. aeruginosa

24 hrs
100





ATCC # 9027


75






26.25
75
1.26
1:2.8571




43.75
37.5
0.94
1:0.8571




55
37.5
1.05
1:0.6818




55
18.75
0.80
1:0.3409




100
18.75
1.25
1:0.1875




55
9.4
0.68
1:0.1709




100
9.4
1.13
1:0.0940




100
4.6
1.06
1:0.0460



48 hrs
150








125






26.25
75
0.78
1:2.8571




32.5
75
0.82
1:2.3077




43.75
75
0.89
1:1.7143




55
75
0.97
1:1.3636




100
75
1.27
1:0.7500




55
37.5
0.67
1:0.6818




100
37.5
0.97
1:0.3750




150
37.5
1.3 
1:0.2500




100
18.75
0.82
1:0.1875




150
18.75
1.15
1:0.1250




150
9.4
1.08
1:0.0627




150
4.6
1.04
1:0.0307



S. aureus

24 hrs
55





ATCC # 6538


30






2.125
15
0.54
1:7.0588




5.5
15
0.60
1:2.7273




10
15
0.68
1:1.5000




26.25
15
0.98
1:0.5714




32.5
15
1.09
1:0.4615




21.25
7.5
0.64
1:0.3529




26.25
7.5
0.73
1:0.2857




32.5
7.5
0.84
1:0.2308




43.75
7.5
1.05
1:0.1714




32.5
3.8
0.72
1:0.1169




43.75
3.8
0.92
1:0.0869




55
3.8
1.13
1:0.0691




55
1.8
1.06
1:0.0327




55
0.94
1.03
1:0.0171



48 hrs
55








30






2.125
15
0.54
1:7.0588




5.5
15
0.60
1:2.7273




10
15
0.68
1:1.5000




26.25
15
0.98
1:0.5714




32.5
15
1.09
1:0.4615




21.25
7.5
0.64
1:0.3529




26.25
7.5
0.73
1:0.2857




32.5
7.5
0.84
1:0.2308




43.75
7.5
1.05
1:0.1714




32.5
3.8
0.72
1:0.1169




43.75
3.8
0.92
1:0.0869




55
3.8
1.13
1:0.0691




55
1.8
1.06
1:0.0327




55
0.94
1.03
1:0.0171





Ca: ppm AI of BIOBAN1246 (7α-ethyldihydro-1H,3H,5H-oxazolo (3,4-C) oxazole)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb


















TABLE 10






Contact






Test Organisms
Time
Ca
Cb
S.I.
Ca:Cb





















A. niger

 3 days
3





ATCC # 16404


37.5






3
2.4
1.06
1:0.8000




3
1.2
1.03
1:0.4000



 7 days
8.75








150






0.525
75
0.56
1:142.8571




0.65
75
0.57
1:115.3846




0.875
75
0.60
1:85.7143




1.1
75
0.63
1:68.1818




2
75
0.73
1:37.5000




3
75
0.84
1:25.0000




4.25
75
0.99
1:17.6471




8.75
9.4
1.06
1:1.0743




6.5
2.4
0.76
1:0.3692




8.75
2.4
1.02
1:0.2743




8.75
1.2
1.01
1:0.1371



C. albicans

48 hrs
4.25





ATCC # 10231


30






4.25
1.86
1.06
1:0.4376




4.25
0.94
1.03
1:0.2212



72 hrs
5.25








60






1.1
30
0.71
1.27.2727




2
30
0.88
1:15.0000




3
30
1.07
1:10.0000




4.25
15
1.06
1:3.5294




4.25
7.5
0.93
1:1.7647




5.25
3.75
1.06
1:0.7143




4.25
1.86
0.84
1:0.4376




5.25
1.86
1.03
1:0.3543




4.25
0.94
0.83
1:0.2212




5.25
0.94
1.02
1:0.1790



Ps. aeruginosa

48 hrs
212.5





ATCC # 9027


125






21.25
75
0.70
1:3.5294




26.25
75
0.72
1:2.8571




43.75
75
0.81
1:1.7143




55
75
0.86
1:1.3636




100
75
1.07
1:0.7500




150
37.5
1.01
1:0.2500




150
18.75
0.86
1:0.1250




212.5
18.75
1.15
1:0.0882




150
9.4
0.78
1:0.0627




212.5
9.4
1.08
1:0.0442




212.5
4.6
1.04
1:0.0216



S. aureus

24 hrs
26.25





ATCC # 6538


15






26.25
0.94
1.06
1:0.0358





Ca: ppm AI of TCMTB (2-(thiocyanomethylthio) benzothiazole)


Cb: ppm AI of MBIT(N-methyl-1,2-benzisothiazolin-3-one)


Ratio: Ca:Cb





Claims
  • 1. A microbicidal composition comprising: (a) N-methyl-1,2-benzisothiazolin-3-one; and(b) at least one microbicide selected from the group consisting of hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine; 4,4-dimethyloxazolidine; sodium hydroxymethylglycinate; myristamidopropyl PG-dimoonium chloride phosphate; octanoic acid; 2-ethylhexyl glycerin; glyceryl monocaprylate; glyceryl monocaprate; 7α-ethyldihydro-1H,3H, 5H-oxazolo (3,4-C) oxazole; and 2-(thiocyanomethylthio)benzothiazole.
  • 2. The microbicidal composition of claim 1 in which said at least one microbicide is hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine, and a ratio of hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0014 to 1:150.
  • 3. The microbicidal composition of claim 1 in which said at least one microbicide is 4,4-dimethyloxazolidine, and a ratio of 4,4-dimethyloxazolidine to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0011 to 1:3.5.
  • 4. The microbicidal composition of claim 1 in which said at least one microbicide is sodium hydroxymethylglycinate, and a ratio of sodium hydroxymethylglycinate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0005 to 1:2.3.
  • 5. The microbicidal composition of claim 1 in which said at least one microbicide is myristamidopropyl PG-dimoonium chloride phosphate, and a ratio of myristamidopropyl PG-dimoonium chloride phosphate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0011 to 1:150.
  • 6. The microbicidal composition of claim 1 in which said at least one microbicide is octanoic acid, and a ratio of octanoic acid to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0046 to 1:0.68.
  • 7. The microbicidal composition of claim 1 in which said at least one microbicide is 2-ethylhexyl glycerin, and a ratio of 2-ethylhexyl glycerin to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.017 to 1:2.3.
  • 8. The microbicidal composition of claim 1 in which said at least one microbicide is glyceryl monocaprylate, and a ratio of glyceryl monocaprylate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0004 to 1:75.
  • 9. The microbicidal composition of claim 1 in which said at least one microbicide is glyceryl monocaprate, and a ratio of glyceryl monocaprate to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0031 to 1:8.8.
  • 10. The microbicidal composition of claim 1 in which said at least one microbicide is 7α-ethyldihydro-1H,3H,5H-oxazolo (3,4-C) oxazole, and a ratio of 7α-ethyldihydro-1H, 3H,5H-oxazolo (3,4-C) oxazole to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.0003 to 1:7.1.
  • 11. The microbicidal composition of claim 1 in which said at least one microbicide is 2-(thiocyanomethylthio)benzothiazole, and a ratio of 2-(thiocyanomethylthio)benzothiazole to N-methyl-1,2-benzisothiazolin-3-one is from 1:0.063 to 1:143.
Priority Claims (1)
Number Date Country Kind
07290901.3 Jul 2007 EP regional