Claims
- 1. A microfluidic device comprising a solid support comprising:
a) a sample inlet port; b) at least one microchannel comprising at least one section with walls comprising magnetic beads and an inner diameter devoid of said beads; c) a sample outlet port.
- 2. A device according to claim 1 wherein said magnetic beads are embedded in said walls.
- 3. A device according to claim 1 wherein said magnetic beads are coated onto the inner surface of said walls.
- 4. A device according to claim 1 wherein said magnetic beads are of a uniform size.
- 5. A device according to claim 1 wherein said magnetic beads are of non-uniform size.
- 6. A device according to claims 1-5 wherein said magnetic beads are ferromagnetic.
- 7. A device according to claims 1-5 wherein said magnetic beads are permanently magnetized.
- 8. A device according to claims 1-5 wherein said magnetic beads are magnetized by electromagnet.
- 9. A device according to claim 1, further comprising a magnet that imparts magnetic property to the magnetic beads.
- 10. A device according to claim 1, further comprising a labeling chamber.
- 11. A device according to claim 1, further comprising a releasing chamber.
- 12. A device according to claim 1, further comprising a buffer inlet port.
- 13. A device according to claim 1, further comprising a waste outlet port.
- 14. A device according to claim 1, further comprising a detection module.
- 15. A device according to claim 14 wherein said detection module comprises:
a) a detection electrode; b) a self-assembled monolayer; c) a binding ligand; d) a detection inlet port to receive said sample.
- 16. A device according to claim 1, further comprising a reagent storage well.
- 17. A device according to claim 1, further comprising a cell handling well.
- 18. A device according to claim 1, further comprising a reaction module.
- 19. A device according to claim 1, further comprising a separation module.
- 20. A device according to claim 1 further comprising a pump.
- 21. A device according to claim 1 further comprising a valve.
- 22. A microfluidic device comprising a solid support comprising:
a) a sample inlet port; b) at least one microchannel comprising a gradient inducing feature coated with a magnetic material; and c) a sample outlet port.
- 23. A device according to claim 22 wherein said microchannel comprises a plurality of gradient inducing features.
- 24. A device according to claim 22 wherein said gradient inducing feature is a sawtooth ridge.
- 25. A device according to claim 22 wherein said gradient inducing feature is a dome.
- 26. A device according to claim 22 wherein said gradient inducing feature has a diameter of between 1 μm and 1000 μm.
- 27. A device according to claim 22 wherein said magnetic material is an iron-nickel alloy.
- 28. A microfluidic device comprising a solid support comprising:
a) a sample inlet port; b) at least one microchannel comprising at least one section filled with magnetic beads; c) a sample outlet port; and d) a detection module comprising:
i) a detection electrode; ii) a self-assembled monolayer; iii) a binding ligand; and iv) a detection inlet port to receive said sample.
- 29. A method to process a target analyte in a sample comprising:
a) provide said target analyte labeled with a magnetic label; and b) introducing said labeled target analyte to a microfluidic device comprising a solid support comprising:
i) a sample inlet port; ii) at least one microchannel comprising at least one section with walls comprising magnetic beads; iii) a sample outlet port; under conditions whereby said labeled target analyte binds to said walls.
- 30. A method according to claim 29, further comprising:
a) washing away other components of said sample from said microchannel.
- 31. A method according to claim 29 or claim 30, further comprising treating the target analyte inside the channel.
- 32. A method according to claim 29 or claim 30, further comprising detecting the target analyte inside the magnetic microchannel.
- 33. A method according to any one of claims 29-31, further comprising eluting the target analyte or the analysis product from said walls.
- 34. A method according to claim 33, wherein said elution is achieved by reversing the electromagnet.
- 35. A method according to claim 33, wherein said elution is achieved by ferrofluid.
- 36. A method according to claim 33, wherein the elution is achieved by chemical disruption.
- 37. A method according to claim 33, wherein the elution is achieved by thermal disruption.
- 38. A method according to claim 29 wherein said target analyte is nucleic acid.
- 39. A method according to claim 29 wherein said target analyte is protein.
- 40. A method according to claim 29 wherein said target analyte is cell.
- 41. A method according to claim 29 wherein said target analyte is labeled in a labeling chamber.
- 42. A method according to claim 29, wherein said target analyte is further treated in a post-treatment module.
- 43. A method to process a target analyte in a sample comprising:
a) providing said target analyte labeled with a magnetic label; and b) introducing said labeled target analyte to a microfluidic device comprising a solid support comprising:
i) a sample inlet port; ii) at least one microchannel comprising a gradient inducing feature coated with a magnetic material; and iii) a sample outlet port; under conditions whereby said labeled target analyte is transported toward said gradient inducing feature.
- 44. A method to process a target analyte in a sample comprising:
a) provide said target analyte labeled with a magnetic label; and b) introducing said labeled target analyte to a microfluidic device comprising a solid support comprising:
i) a sample inlet port; ii) at least one microchannel comprising at least one section filled with magnetic beads; iii) a sample outlet port; and iv) a detection module comprising:
1) a detection electrode 2) a self-assembled monolayer; 3) a binding ligand; and 4) a detection inlet port to receive said sample. under conditions whereby said labeled target analyte binds to said channel.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0001] The invention resulted in part from work on U.S. Government contract 70NANB9H3012 and DARPA #MDA972-01-3-0001.