Claims
- 1. Apparatus for determining the effect of test samples of compounds on ion-transfer channels of a membrane, comprising: a microperfusion chamber assembly comprising: a base, an aperture in said base, and transparent means over a bottom of said base, said aperture and said transparent means cooperating to define the side walls and a bottom of a microperfusion chamber, said microperfusion chamber being open to the atmosphere at a top thereof, a reference electrode arranged to contact a liquid in said chamber, means for introducing the liquid into said chamber, means for aspirating the liquid from said chamber, and means for electrically connecting said reference electrode to an electrical measuring device.
- 2. A microperfusion chamber assembly according to claim 1, wherein said transparent means which cooperates with said aperture to define the bottom of said chamber is a transparent coverslip.
- 3. A microperfusion chamber assembly according to claim 1, wherein said base comprises silver having a coating of a silver halide deposited over at least the surface of the aperture defining the side walls of said microperfusion chamber, thereby providing a reference electrode in said base adapted to make electrical contact with a liquid contained in said chamber.
- 4. A microperfusion chamber assembly according to claim 1, wherein said base comprises silver having a coating of silver chloride deposited over at least the surface of the aperture defining the side walls of said microperfusion chamber.
- 5. A microperfusion chamber assembly according to claim 4, wherein said base comprises silver having a coating of silver chloride deposited over its entire surface including the side walls of said chamber.
- 6. A microperfusion chamber assembly according to claim 5, wherein said microperfusion chamber is cylindrical, frustoconical, or ellipsoidal.
- 7. A microperfusion chamber assembly according to claim 5, wherein the volume of said microperfusion chamber is between about 5 microliters to about 50 microliters.
- 8. A microperfusion chamber assembly according to claim 5, wherein the volume of said microperfusion chamber is between about 10 microliters to about 15 microliters.
- 9. A microperfusion chamber assembly according to claim 5, wherein the volume of said microperfusion chamber is between about 10 microliters to about 12 microliters.
- 10. A microperfusion chamber assembly according to claim 1, wherein said base is formed of a non-electrically conductive material.
- 11. A microperfusion chamber assembly according to claim 10, wherein said base comprises a plastic material.
- 12. A microperfusion chamber assembly according to claim 11, wherein said plastic material is polymethyl methacrylate, polystyrene, polyvinyl chloride, or polycarbonate.
- 13. A microperfusion chamber assembly according to claim 1, wherein said electrode comprises a silver wire surrounded by a mixture of particulate silver and a silver halide.
- 14. A microperfusion chamber assembly according to claim 13, wherein said electrode comprises a silver wire surrounded by a mixture of particulate silver and silver chloride.
- 15. A microperfusion chamber assembly according to claim 14, wherein said particulate silver and silver chloride are affixed to said silver wire.
- 16. A microperfusion chamber according to claim 1, wherein said means for introducing a liquid into said microperfusion chamber is a groove provided in said base cooperating with said transparent means over the bottom of the base to define a channel, a proximal end of said channel communicating with said microperfusion chamber, and a second smaller aperture provided at a top of said base communicating with a distal end of said channel and adapted to have an inflow duct connected thereto for introducing the liquid into said microperfusion chamber.
- 17. A microperfusion chamber assembly according to claim 16, wherein the volume of said microperfusion chamber is between about 5 microliters to about 50 microliters.
- 18. A microperfusion chamber assembly according to claim 16, wherein the volume of said microperfusion chamber is between about 10 microliters to about 15 microliters.
- 19. A microperfusion chamber assembly according to claim 16, wherein the volume of said microperfusion chamber is between about 10 microliters to about 12 microliters.
Priority Claims (1)
Number |
Date |
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Kind |
1252/94 |
Oct 1994 |
DKX |
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Parent Case Info
The present application is a division of our prior-filed application Ser. No. 08/836,094, filed Apr. 25, 1997 which is 371 of PCT/EP95/02204, Jun. 7, 1995 now U.S. Pat. No. 6,063,260.
US Referenced Citations (10)
Foreign Referenced Citations (2)
Number |
Date |
Country |
4305405 |
May 1994 |
DEX |
8502201 |
May 1985 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Patent Abstracts of Europe (DE 04305405 C1) May 26, 1994 (Assignee: Hering). |
Divisions (1)
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Number |
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Parent |
836094 |
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