Patent Application
20150043062
1. Technical Field
The disclosure relates to a microscope apparatus having a plurality of optical units of the same type and to a method of controlling an electrically driven optical unit of the microscope apparatus.
2. Related Art
Presently, microscope apparatuses, which enable observation of a micro sample by magnification and is able to record observed images as photographs or video images, are used in a wide range of fields, such as biological fields and industrial fields. In the biological fields, such a microscope apparatus is used, for example, in observation of a biological specimen, such as a biological cell or cell piece and in time lapse observation in combination with a culturing apparatus for culturing a cell.
Since microscope apparatuses are used in the above mentioned various fields, each part thereof is often unitized, and according to combinations of plural units, microscope apparatuses are able to be used for various purposes.
Conventionally, a microscope system has been disclosed, which includes a display content changing means for displaying operating icons only for units that connected to a microscope main body thereof, without including controllers independently provided for respective connection units in a microscope apparatus thereof, to and from which various microscopy units and image capturing units are attachable and detachable (for example, see Japanese Patent Application Laid-open No. H07-199077).
Further, a microscope apparatus has been disclosed that controls a lamp output, a field stop, and an aperture stop in such a way that optimum illumination light, illumination light's irradiation range, and specimen's contrast can be achieved when switching between microscopies (for example, see Japanese Patent Application Laid-open No. H08-211295).
In some embodiments, a microscope apparatus includes: a stage on which a sample is configured to be placed; a microscope main body unit that holds the stage; an objective lens arranged below the sample; one or a plurality of light sources configured to emit light for exciting the sample; one or a plurality of cube turrets that holds a plurality of fluorescent cubes and is configured to arrange the plurality of fluorescent cubes selectively on an observation optical path; an imaging element configured to capture an image of the sample; a plurality of shutters arranged on an illumination optical path and configured to switch between irradiation of the sample with the light emitted from the one or the plurality of light sources and blocking of the light; one input unit configured to receive inputs of operation instructions for the plurality of shutters; a storage unit that stores operation conditions of the plurality of shutters corresponding to the operation instructions received by the input unit; an operation controller configured to control operations of the plurality of shutters; and a controller configured to acquire, from the storage unit, the operation conditions corresponding to the operation instructions received by the input unit, and to output instruction signals to the operation controller based on the acquired operation conditions in order to change conditions of the plurality of shutters. One of the plurality of shutters, and the other of the plurality of shutters or the one or the plurality of cube turrets are arranged on a same illumination optical path.
The above and other features, advantages and technical and industrial significance of this invention will be better understood by reading the following detailed description of presently preferred embodiments of the invention, when considered in connection with the accompanying drawings.
Embodiments of a microscope apparatus according to the present invention will be described below in detail, based on the drawings. The present invention is not limited by these embodiments and various changes may be made without departing from the scope of the present invention.
In the microscope main body unit 1: a shutter 8A that switches between irradiation of the sample 2 with light emitted from the light source 5 and blocking of the light; a cube turret 10 having therein built-in optical elements, such as a fluorescent cube and a mirror; a mirror 11 that reflects the light reflected from the sample 2 towards the imaging element 7; a beam splitter 12 that splits the light reflected from the mirror 11; and a mirror 13 that reflects the light split by the beam splitter 12 to the eyepiece 6, are arranged as appropriate.
Further, in the microscope apparatus 100: a filter wheel 15 having therein a plurality of built-in excitation filters 14, and a shutter 8B synchronized with the filter wheel 15 are connected on an optical path of the light emitted from the light source 5; and a filter wheel 18 having therein a plurality of built-in absorption filters 17 is arranged on an observation optical path. The filter wheel 18 also operates in synchronization with the filter wheel 15. In the first embodiment, the shutter 8A is built in the microscope apparatus 100, the shutter 8B is a unit externally attached together with the filter wheel 15, and an operational speed of the shutter 8B is set to be faster than the shutter 8A.
The shutter 8A and shutter 8B are connected to a main controller 30 via a shutter operation controller 19A and a shutter operation controller 19B. The shutter operation controller 19A and shutter operation controller 19B control, based on control signals transmitted from the main controller 30, motors to open or close the shutter 8A and shutter 8B respectively.
The filter wheel 15 and filter wheel 18 are connected to the main controller 30 via a filter wheel operation controller 21 and a filter wheel operation controller 22. The filter wheel operation controller 21 and filter wheel operation controller 22 control, based on control signals transmitted from the main controller 30, motors to operate the filter wheel 15 and filter wheel 18 respectively.
The cube turret 10 is connected to the main controller 30 via a cube turret operation controller 23. Based on a control signal transmitted from the main controller 30, the cube turret operation controller 23 operates the cube turret 10 and controls a motor to insert a cube onto the observation optical path.
An input unit 24 receives an operation instruction, that is, an open or close instruction, for a shutter (shutter 8A or shutter 8B) from a user.
A storage unit 25 stores operation conditions of the shutter 8A and shutter 8B corresponding to operation instructions for a shutter (shutter 8A or shutter 8B) input by the input unit 24.
The main controller 30 is connected to each unit of the microscope apparatus 100 and transmits control signals that control operations of each unit. Further, the main controller 30 acquires, from the storage unit 25, the operation conditions corresponding to the operation instruction for the shutter 8A or shutter 8B input by the user via the input unit 24, and based on the acquired operation conditions, outputs control signals for operating the shutter 8A and shutter 8B in conjunction with each other, to the shutter operation controller 19A and shutter operation controller 19B, respectively.
Next, with reference to the drawings, a sequence of operations of the microscope apparatus 100 upon its initiation according to the first embodiment of the present invention will be described.
As illustrated in
Thereafter, initialization of the shutter 8A and shutter 8B is performed (S102). By this initialization, the shutter 8A and shutter 8B are moved to their initial position. If, for example, the shutter 8A and shutter 8B are set to be open as their initial position, the main controller 30 transmits control signals of the initial position to the shutter operation controller 19A and shutter operation controller 19B. Thereby, the shutter 8A and shutter 8B are opened. The initial position may be a position that is determined beforehand, or a position upon the last time's very last usage may be the initial position. Further, the initial position may be defined as having all of the shutters closed.
Subsequently, a sequence of operations of the shutter 8A and shutter 8B according to the first embodiment of the present invention will be described with reference to the drawings.
The user inputs an operation of a shutter via the input unit 24 (step S201). The input unit 24 has a selector, such as a button or switch, which opens or closes the shutter (shutter 8A or shutter 8B) and the user inputs the operation of the shutter by the selector.
The main controller 30 acquires the operation conditions for an operation instruction of the shutter 8A or shutter 8B from the user by the input unit 24 (step S202). For example, the table of the operation conditions as illustrated in
After acquiring the operation conditions, the main controller 30 outputs, based on the acquired operation conditions, control signals (operation instructions) for operations of the shutter 8A and shutter 8B to the shutter operation controller 19A and shutter operation controller 19B respectively (step S203). For example, if “close” is selected and input as the operation, the main controller 30 outputs control signals to close the shutter 8A and shutter 8B to the shutter operation controller 19A and shutter operation controller 19B respectively.
The shutter operation controller 19A and shutter operation controller 19B control, based on the received control signals, motors to operate the shutter 8A and shutter 8B (step S204). Thereby, by a single operation input by the user, the shutter 8A and shutter 8B are operated in conjunction with each other. In this specification, “in conjunction with” includes a case of simultaneous operation as well as a case of sequential operation.
The first embodiment has the effect of, even if the two shutters 8A and 8B are arranged on the same optical path, on which the irradiation light emitted from the one light source 5 is incident, by a single operation input process: allowing release or shut-off of the optical path; allowing complicated manipulation to be omitted, the complicated manipulation including determining a position where the shutter 8A and shutter 8B do not hinder observation between each other and individually inputting operations of the shutters; and allowing contribution to improvement of operability for a user.
In the first embodiment, although a case in which the two shutters 8A and 8B are arranged on the same optical path has been described as an example, as long as there are two or more, even if three or more shutters are connected, similarly by a single operation, the three or more shutters are able to be manipulated in conjunction with one another and time and effort required for the manipulation are able to be reduced.
Further, as a modified example 1-1 of the first embodiment, a microscope apparatus can be exemplified which causes a shutter for irradiating the eyepiece 6 with reflected light or shutting off the reflected light to operate in conjunction with another shutter.
As illustrated in
In the storage unit 25 of the microscope apparatus 100A, a table of operations conditions as illustrated in
According to the modified example 1-1, not only for the plurality of shutters arranged on the same optical path but also for the shutter arranged on the eyepiece side, the plural shutters are able to be operated in conjunction with one another by a single operation input.
A microscope apparatus according to a second embodiment is different from that of the first embodiment in that a plurality of cube turrets are arranged on the same observation optical path, shutters that switch between irradiation with irradiation light from individual light sources with respect to the cube turrets and blocking of the irradiation light are respectively arranged, and the plurality of cube turrets and the plurality of shutters are operated in conjunction with one another by a single operation instruction.
As illustrated in
Illumination light from a light source 5A is incident on the cube turret 10A and the shutter 8A, which switches between irradiation with the illumination light and blocking of the illumination light, is arranged on an optical path of the illumination light from the light source 5A. Further, illumination light from a light source 5D is incident on the cube turret 10D, and a shutter 8D, which switches between irradiation with the illumination light and blocking of the illumination light, is arranged on an optical path of the illumination light from the light source 5D. The shutter 8A and shutter 8D are connected to the main controller 30 via the shutter operation controller 19A and a shutter operation controller 19D.
The cube turrets 10A and 10D are, as illustrated in
Subsequently, a sequence of operations of the cube turrets 10A and 10D according to the second embodiment of the present invention will be described with reference to the drawings.
As to operations of the microscope apparatus 200 upon its initiation, similarly to the above described flow chart illustrated in
A user inputs a selection of a cube via the input unit 24 (step S301). The input unit 24 has a selector, such as buttons or switches to select cubes A-1, A-2, . . . , and A-8, and D-1, D-2, . . . , and D-8 held by the cube turret 10A and cube turret 10D and the user inputs a cube selection via the selector.
The main controller 30 acquires, from the storage unit 25, operation conditions corresponding to the user's cube selection instruction by the input unit 24 (step S302). For example, in the storage unit 25, a table of operation conditions as illustrated in
After acquiring the operation conditions, the main controller 30 outputs, based on the acquired operation conditions, control signals for operations of the cube turret 10A and cube turret 10D as well as the shutter 8A and shutter 8D to the cube turret operation controller 23A and cube turret operation controller 23D, as well as the shutter operation controller 19A and shutter operation controller 19D, respectively (step S303).
Based on the received control signals, the shutter operation controller 19A and shutter operation controller 19D control motors to operate the shutter 8A and shutter 8D (step S304).
Thereafter, the cube turret operation controller 23A and cube turret operation controller 23D control motors to operate the cute turret 10A and cube turret 10D, based on the received control signals (step S305). As above, by the single cube selection input by the user, the cube turret 10A and cube turret 10D, as well as the shutter 8A and shutter 8D are operated in conjunction with one another.
The second embodiment has the effect of, even if two cube turrets are connected on the same observation optical path, by a single cube selection input process: allowing two cube turrets and two shutters to be operated in conjunction with one another and operated to positions at which correct observation is possible; allowing complicated manipulation to be omitted, the complicated manipulation including determining a position where the two cube turrets and two shutters do not hinder observation among one another and inputting operations of respective cube turrets and respective shutters; and allowing operability for a user to be greatly improved.
In the second embodiment, a case in which the two cube turrets 10A and 10D are arranged on the same observation optical path has been described as an example, but as long as there are two or more, also in a case in which three or more cube turrets are connected, similarly, by a single operation, the three or more cube turrets and shutters are able to be manipulated in conjunction with one another and time and effort required for the manipulation are able to be reduced.
A microscope apparatus according to a third embodiment has the same configuration as that of the microscope apparatus 100 according to the first embodiment of
In the third embodiment, the input unit 24 has a selector, such as buttons or switches, to select the shutter 8A and shutter 8B, and a user selects and inputs the shutter 8A or shutter 8B to be used, by using the selector. Further, the input unit 24 has a selector, such as buttons or switches, to open or close the shutter 8A and shutter 8B, and enables input of an operation to open or close the shutter 8A or shutter 8B selected by the user via the selector. Furthermore, the shutters not being selected are opened.
Subsequently, a sequence of operations of the shutter 8A and shutter 8B according to the third embodiment of the present invention will be described with reference to the drawings.
As for operations of the microscope apparatus 100 upon its initiation, similarly to the above described flow chart illustrated in
A user inputs a selection of the shutter 8A or shutter 8B via the input unit 24 (step S401). The input unit 24 has a selector, such as buttons or switches, to select the shutter 8A or shutter 8B, and the user inputs the shutter 8A or shutter 8B selection via the selector.
The main controller 30 acquires, from the storage unit 25, operation conditions corresponding to an instruction to select the shutter 8A or shutter 8B by the user via the input unit 24 (step S402). For example, a table of operation conditions is stored in the storage unit 25, and if the user selects the shutter 8A, the shutter 8A is to be closed and the shutter 8B is to be opened (see
After acquiring the operation conditions, the main controller 30 outputs, based on the acquired operation conditions, control signals for operations of the shutter 8A and shutter 8B to the shutter operation controller 19A and shutter operation controller 19B, respectively (step S403). For example, if the shutter 8A is selected and input, the main controller 30 outputs control signals to close the shutter 8A and open the shutter 8B respectively to the shutter operation controller 19A and shutter operation controller 19B.
The shutter operation controller 19A and shutter operation controller 19B control, based on the received control signals, motors to operate the shutter 8A and shutter 8B (step S404). Thereby, by the user's shutter selection and input, the shutter 8A and shutter 8B are operated in conjunction with each other.
The third embodiment has the effect of, even if the two shutters 8A and 8B are arranged on the same optical path, on which the illumination light emitted from the one light source 5 is incident, by a single process of selecting and inputting the shutter 8A or shutter 8B: allowing release or shut-off of the optical path; allowing complicated manipulation to be omitted, the complicated manipulation including determining a position where the shutter 8A and shutter 8B do not hinder observation between each other and individually inputting operations of the shutters; and allowing contribution to improvement of operability for a user.
In the third embodiment, the case in which the two shutters 8A and 8B are arranged on the same optical path has been described as an example, but as long as there are two or more, even if three or more shutters are connected, similarly, by a single operation, the three or more shutters are able to be manipulated in conjunction with one another, and time and effort required for the manipulation are able to be reduced.
If, after the shutter 8A is selected, the shutter 8B operates in conjunction with another unit in another sequence, for example, if the shutter 8B operates in conjunction with the filter wheel 15, the shutter 8B may be closed, such that the shutter 8B is not at the shutter 8B's position (not open) after the selection of the shutter 8A. In that case, the main controller 30 detects end of the operation of the filter wheel 15, and transmits a control signal to open the shutter 8B to the shutter operation controller 19B. Thereby, even if the other sequence causes it to be in a state different from the position after the shutter selection, automatic return to the position after the shutter selection becomes possible, and there is an effect that contribution to improvement of operability for a user is possible.
A microscope apparatus according to a fourth embodiment has the same configuration as that of the microscope apparatus 100 according to first embodiment of
A sequence of operations of the shutter 8A and shutter 8B, as well as the cube turret 10 according to the fourth embodiment of the present invention will be described with reference to the drawings.
As for operations of the microscope apparatus 100 upon its initiation, similarly to the above described flow chart illustrated in
A user inputs a selection of a cube via the input unit 24 (step S601). The input unit 24 has a selector, such as buttons or switches, to select a cube, and the user inputs a cube selection via the selector.
After the input of the cube selection, the main controller 30 acquires operation conditions from the storage unit 25 (step S602). The operation conditions of the fourth embodiment are that after the shutter 8A and shutter 8B are closed in conjunction with each other and the cube selected in the initialization process is arranged on the observation optical path, the shutter 8A and shutter 8B are opened.
The main controller 30 transmits control signals for closing the shutter 8A and shutter 8B to the shutter operation controller 19A and shutter operation controller 19B, based on the acquired operation conditions, and causes the shutter 8A and shutter 8B to close in conjunction with each other (step S603). By causing the shutter 8A and shutter 8B to close when a cube switch over operation is performed, discoloration of the sample 2 is able to be prevented.
Thereafter, the main controller 30 transmits, to the cube turret operation controller 23, an operation instruction for the cube selected in the initialization process, that is, a control signal to arrange the selected cube on the observation optical path (step S604).
The cube turret operation controller 23 that has received the control signal operates the cube turret 10 and places the selected cube on the observation optical path (step S605).
The main controller 30 transmits control signals to open the shutter 8A and shutter 8B to the shutter operation controller 19A and shutter operation controller 19B, and based on the received control signals, the shutter operation controller 19A and shutter operation controller 19B control motors to open the shutter 8A and shutter 8B (step S606).
According to the fourth embodiment, when the two shutters 8A and 8B are arranged on the same optical path, on which irradiation light emitted from the one light source 5 is incident, by a single operation, with a cube selection input being a trigger, the two shutters 8A and 8B are able to be operated in conjunction with each other, and while being able to omit complicated manipulation of inputting operations of the shutters individually, discoloration of the sample 2 is able to be prevented.
In the fourth embodiment, the case in which the two shutters 8A and 8B are arranged on the same optical path has been described as an example, but as long as there are two or more, even if three or more shutters are connected, by a single operation, with a cube selection input being a trigger, the three or more shutters are able to be manipulated in conjunction with one another and time and effort required for the manipulation are able to be reduced.
Further, in the fourth embodiment, although the shutter 8A and shutter 8B are closed in conjunction with each other and after the cube selection operation, further the shutter 8A and shutter 8B are opened in conjunction with each other, the operation conditions may be that after operating a close operation of the shutter 8A and an open operation of the shutter 8B in conjunction with each other, and after a cube selection operation, only the shutter 8A is opened further. Or, the operation conditions may be that, after operating an open operation of the shutter 8A and a close operation of the shutter 8B in conjunction with each other, and after a cube selection operation, only the shutter 8B is opened further.
A microscope apparatus according to a fifth embodiment includes, in addition to a plurality of shutters arranged on an optical path on which irradiation light from one epi-illumination light source is incident, a shutter that switches between irradiation with illumination light from a transmitted light source and blocking of the illumination light, and the microscope apparatus operates the plurality of shutters in conjunction with one another with an input of a microscopy being a trigger.
As illustrated in
The input unit 24 of the microscope apparatus 300 has a selector, such as buttons or switches, to select a microscopy, for example, a transmitted-light microscopy, an epi-illumination microscopy, or a simultaneous microscopy for performing the transmitted-light microscopy and epi-illumination microscopy simultaneously, and a user inputs a microscopy selection via the selector.
The storage unit 25 of the microscope apparatus 300 stores a table of operation conditions of the shutter 8A, shutter 8B, and shutter 8E, corresponding to microscopy selections by the input unit 24.
A sequence of operations of the shutter 8A, shutter 8B, and shutter 8E according to the fifth embodiment of the present invention will be described with reference to the drawings.
As for operations of the microscope apparatus 300 upon its initiation, similarly to the above described flow chart illustrated in
A user selects and inputs a microscopy via the input unit 24 (step S701).
After the input of the microscopy selection, the main controller 30 acquires operation conditions from the storage unit 25 (step S702). In the fifth embodiment, a table of operation conditions as illustrated in
If the initialization indicates the transmitted-light microscopy, in order to perform a close operation for the shutter 8E that is open, the main controller 30 transmits a control signal for closing the shutter 8E to the shutter operation controller 19E to close the shutter 8E (step S703). Thereby, discoloration of the sample 2 is able to be prevented.
Thereafter, the main controller 30 transmits operation conditions for the microscopy selected in step S701, that is, control signals to open the shutter 8A and shutter 8B, to the shutter operation controller 19A and shutter operation controller 19B (step S704).
The shutter operation controller 19A and shutter operation controller 19B, which have received the control signals, control motors to open the shutter 8A and shutter 8B in conjunction with each other (step S705).
According to the fifth embodiment, because the plurality of shutters arranged on the optical path, on which the illumination light from the epi-illumination light source is incident, and the shutter that switches between irradiation with the illumination light from the transmitted light source and blocking of the illumination light are able to be operated in conjunction with one another, with the input of the microscopy being the trigger, while omitting complicated manipulation of inputting operations of the shutters individually, the transmitted-light microscopy and epi-illumination microscopy are able to be switched over to each other.
In the fifth embodiment, from the viewpoint of prevention of discoloration of the sample 2, all of the shutters are closed once, but all of the three shutters may be operated in conjunction with one another.
In a microscope apparatus according to a sixth embodiment, two cube turrets are arranged on the same observation optical path; and two shutters, which switch between irradiation with illumination light from an individual light source with respect to each cube turret and blocking of the illumination light, are arranged, and the plural shutters are caused to be operated in conjunction with each other by an input of an optical path (light source) selection.
As illustrated in
On the cube turret 10A, illumination light from the light source 5A is incident, and on a first optical path of the illumination light from the light source 5A, the shutter 8A and shutter 8B, which switch between irradiation with the illumination light and blocking of the illumination light, are arranged. Further, illumination light from the light source 5D is incident on the cube turret 10D, and the shutter 8D and a shutter 8F, which switch between irradiation with the illumination light and blocking of the illumination light, are arranged on a second optical path of the illumination light from the light source 5D. The shutter 8A and shutter 8B are connected to the main controller 30 via the shutter operation controller 19A and shutter operation controller 19B, and the shutter 8D and shutter 8F are connected to the main controller 30 via the shutter operation controller 19D and shutter operation controller 19F. Further, the cube turret 10A and cube turret 10D are connected to the main controller 30 via a cube turret operation controller 23A and a cube turret operation controller 23D.
On the first optical path of the light emitted from the light source 5A, a filter wheel 15B having therein a plurality of excitation filters 14B is arranged, and the shutter 8B is synchronized with the filter wheel 15B. Further, on the second optical path of the light emitted from the light source 5D, a filter wheel 15F having therein a plurality of excitation filters 14F is arranged, and the shutter 8F is synchronized with the filter wheel 15F. The filter wheel 15B and filter wheel 15F are connected to the main controller 30 via the filter wheel operation controller 21B and the filter wheel operation controller 21F.
Further, on the observation optical path, a filter wheel 18A having therein a plurality of absorption filters 17A and a filter wheel 18F having therein a plurality of absorption filters 17F are arranged. The filter wheel 18A operates in synchronization with the filter wheel 15B, and the filter wheel 18F operates in synchronization with the filter wheel 15F. The filter wheel 18A and filter wheel 18F are connected to the main controller 30 via the filter wheel operation controller 22A and the filter wheel operation controller 22F.
A sequence of operations of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F according to the sixth embodiment of the present invention will be described with reference to the drawings.
As for operations of the microscope apparatus 400 upon its initiation, similarly to the above described flow chart illustrated in
A user inputs a selection of an optical path (light source) to be used, via the input unit 24 (step S801).
After the input of the selection of the optical path, the main controller 30 acquires operation conditions from the storage unit 25 (step S802). In the sixth embodiment, a table of operations conditions as illustrated in
The main controller 30 transmits, to the shutter operation controller 19A, shutter operation controller 19B, shutter operation controller 19D, and shutter operation controller 19F, control signals to open the shutter 8A and shutter 8B on the first optical path and to close the shutter 8D and shutter 8F on the second optical path (step S803).
The shutter operation controller 19A, shutter operation controller 19B, shutter operation controller 19D, and shutter operation controller 19F, which have received the control signals, control motors, such that the shutter 8A and shutter 8B are opened and the shutter 8D and shutter 8F are closed, in conjunction with each other (step S804).
Thereafter, if the user inputs a shutter operation via the input unit 24, similarly to the flow chart illustrated in
In the sixth embodiment, even if there are two light sources, and two shutters are connected on an optical path from each light source, by a user's selection of an optical path, a plurality of shutters are able to be operated in conjunction with each other, and shut-off of an optical path not being used in the observation becomes possible. Further, by an input of one shutter operation, a plurality of shutters are able to be operated in conjunction with one another, and release and shut-off of only an optical path being used in the observation become possible.
A microscope apparatus according to a seventh embodiment has the same configuration as that of the microscope apparatus 400 according to the sixth embodiment illustrated in
A sequence of operations of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F according to the seventh embodiment of the present invention will be described with reference to the drawings. First, operations for selecting a shutter in each optical path will be described. In the shutter selection, a selection of a shutter is performed for each of the first optical path and second optical path.
As for operations of the microscope apparatus 400 upon its initiation, similarly to the above described flow chart illustrated in
A user inputs a selection of a shutter to be used on the first optical path, via the input unit 24 (step S901).
The main controller 30 acquires operation conditions for the shutter selected in the first optical path from the storage unit 25 (step S902). In the seventh embodiment, a table of operation conditions of the first optical path as illustrated in
The main controller 30 transmits, to the shutter operation controller 19A and shutter operation controller 19B, control signals to operate the shutter 8A and shutter 8B under the acquired operation conditions (step S903) and the shutter operation controller 19A and shutter operation controller 19B, which have received the control signals, control motors to operate the shutter 8A and shutter 8B in conjunction with each other (step S904).
The main controller 30 transmits control signals to operate the shutter 8D and shutter 8F under the acquired operation conditions to the shutter operation controller 19D and shutter operation controller 19F (step S1003), and the shutter operation controller 19D and shutter operation controller 19F, which have received the control signals, control motors to operate the shutter 8D and shutter 8F in conjunction with each other (step S1004).
Thereafter, the main controller 30 determines whether or not the selected shutter on the non-selected optical path is open (step S1102). If the main controller 30 determines that the selected shutter on the non-selected optical path is open (step S1102; Yes), the main controller 30 transmits a control signal to the shutter operation controller to close the selected shutter (step S1103) and proceeds to step S1104. On the contrary, if the main controller 30 determines that the selected shutter on the non-selected optical path is not open, that is, is closed (step S1102; No), the main controller 30 proceeds to step S1104.
The main controller 30 determines whether or not the selected shutter on the selected optical path is closed, after performing shutter control of the non-selected optical path (step S1104). If the main controller 30 determines that the selected shutter on the selected optical path is closed (step S1104; Yes), the main controller 30 causes the selected shutter to open by transmitting a control signal to the shutter operation controller (step S1105) and ends the shutter operation process. On the contrary, if the main controller 30 determines that the selected shutter on the non-selected optical path is not closed, that is, is open (step S1104; No), the main controller 30 ends the shutter operation process.
By the above described shutter operation process, if, for example, the shutter 8B is selected as the shutter to be used in the first optical path and the shutter 8F is selected as the shutter to be used in the second optical path, when the first optical path is selected by the user, the shutter 8A, shutter 8B, and shutter 8D are opened and the selected shutter 8F is kept closed. Further, if the second optical path is selected by the user, the shutter 8A, shutter 8D, and shutter 8F are opened and the selected shutter 8B is kept closed.
According to the seventh embodiment, even if there are two light sources and two shutters are connected on an optical path from each light source, by a user's selection of an optical path, a plurality of shutters are able to be operated in conjunction with one another and shut-off of an optical path not being used in the observation becomes possible. Further, by an input of one shutter operation, a plurality of shutters are able to be operated in conjunction with one another, and release and shut-off of only an optical path being used in the observation become possible.
In the seventh embodiment, although the case in which two shutters are arranged on each of the two optical paths has been described as an example, the number of shutters connected on each optical path just needs to be two or more, and even if three or more shutters are connected, similarly, just by one operation, the three or more shutters are able to be manipulated in conjunction with one another, and time and effort required for the manipulation are able to be reduced.
A microscope apparatus according to an eighth embodiment has the same configuration as that of the third embodiment in that it is operated such that a selected shutter is opened or closed and all of non-selected shutters are opened, but is different from the third embodiment in that the shutter operation controller 19A and shutter operation controller 19B have storage units. FIG. 31 is a diagram illustrating a schematic configuration of the microscope apparatus according to the eighth embodiment of the present invention.
In a microscope apparatus 500 according to the eighth embodiment, a shutter operation controller 19A has a storage unit 191A that stores speed parameters of the shutter 8A. Further, the shutter operation controller 19B has a storage unit 191B that stores speed parameters of the shutter 8B. The speed parameters are parameters related to operations of shutters and include information such as speeds related to opening and closing of shutters.
Subsequently, a sequence of operations of the shutter 8A and shutter 8B according to the eighth embodiment of the present invention will be described with reference to the drawings.
As illustrated in
Thereafter, initialization of the shutter 8A and shutter 8B is performed (S1302). By the initialization, the shutter and shutter 8B are moved to their initial position. If, for example, the shutter 8A and shutter 8B are set to be open as their initial position, the main controller 30 transmits control signals for the initial position to the shutter operation controller 19A and shutter operation controller 19B. Thereby, the shutter 8A and shutter 8B are opened. The initial position may be determined beforehand, or may be a position upon the last time's very last usage. Further, the initial position may be defined as having all of the shutters closed.
Thereafter, the main controller 30 refers to the storage unit 191A and storage unit 191B and acquires the speed parameters of the shutter 8A and shutter 8B respectively (step S1303). The main controller 30 selects, from the shutter 8A and shutter 8B, a shutter for which the acquired speed parameter is larger (maximum) (step S1304), and registers the selected shutter as a high speed shutter (a shutter to be controlled) in the storage unit 25 (step S1305).
The main controller 30 acquires operation conditions from the storage unit 25, after registering the high speed shutter (step S1306). For example, in the storage unit 25, a table of operation conditions as illustrated in
After acquiring the operation conditions, the main controller 30 outputs, based on the acquired operation conditions, control signals for operations of the shutter 8A and shutter 8B respectively to the shutter operation controller 19A and shutter operation controller 19B (step S1307).
The shutter operation controller 19A and shutter operation controller 19B control, based on the received control signals, motors to operate the shutter 8A and shutter 8B respectively (step S1308). Thereby, the shutter 8A and shutter 8B are operated in conjunction with each other.
The eighth embodiment has the effect of, even if the two shutters 8A and 8B are arranged on the optical path, on which the illumination light emitted from the one light source 5 is incident, by referring to the storage unit 191A and storage unit 191B and registering one of the shutter 8A and shutter 8B as the high-speed shutter: allowing release or shut-off of the optical path; allowing complicated manipulation of determining a position where observation is not hindered between the shutter 8A and shutter 8B and inputting operations of the shutters individually to be omitted; and allowing contribution to improvement of a user's operability.
Further, according to the eighth embodiment, even if a user does not perform input of a shutter selection, a shutter is registered and operated such that only a high speed shutter is operated to open or close automatically, and thus selection and input by the user is able to be omitted and release or shut-off of an optical path becomes possible.
In the eighth embodiment, although the case in which the two shutters 8A and 8B are arranged on the same optical path has been described as an example, as long as there are two or more, even if three or more shutters are connected, similarly, by a single operation, the three or more shutters are able to be manipulated in conjunction with one another and time and effort required for the manipulation is able to be reduced.
Further, in the eighth embodiment, for example, if the shutter 8B registered as the high speed shutter is operated in conjunction with the filter wheel 18 and is closed when the conjunction operation is finished, the main controller 30 causes the shutter 8A to close and causes the shutter 8B to open when the main controller 30 detects the finishing of the conjunction operation. Thereby, even if the shutter 8B operates in conjunction with another unit contrary to a user's intention, the shutter 8B is open when the conjunction operation is finished. Thus, it is possible to release or shut-off an optical path without the user confirming a condition of the shutter upon finishing of the operation, and to contribute to improvement of the user's operability. If the optical path is opened or closed by a user's input of an operation, the opening or closing of the optical path is performed by the shutter 8A which is not registered as the high speed shutter. Further, the same holds true for an operation in conjunction with another unit other than the filter wheel 18.
Further, as a modified example 8-1 of the eighth embodiment, a microscope apparatus may be exemplified, which is different from the eight embodiment in that two cube turrets are arranged on the same observation optical path, two shutters that switch between irradiation with illumination light from an individual light source with respect to each cube turret and blocking of the illumination light are arranged, and the plural shutters are operated in conjunction with one another by an input of an optical path selection.
A sequence of operations of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F according to the modified example 8-1 will be described with reference to the drawings. First, operations for selecting a high speed shutter in each optical path will be described. In the shutter selection, a selection of a high speed shutter is performed for each of the first optical path and second optical path.
As illustrated in
Thereafter, initialization of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F is performed (S1502). By the initialization, the shutter 8A, shutter 8B, shutter 8D, and shutter 8F are moved to their initial position. If, as the initial position, for example, the shutter 8A, shutter 8B, shutter 8C, and shutter 8F are set to be open, the main controller 30 transmits control signals for the initial position to the shutter operation controller 19A, shutter operation controller 19B, shutter operation controller 19D, and shutter operation controller 19F. Thereby, the shutter 8A, shutter 8B, shutter 8D, and shutter 8F are opened. The initial position may be determined beforehand, or the initial position may be a position upon the last time's very last usage. Further, the initial position may be defined as having all of the shutters closed.
Thereafter, the main controller 30 refers to the storage unit 191A, storage unit 191B, storage unit 191D, and storage unit 191F, and acquires speed parameters of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F, respectively (step S1503). The main controller 30 first selects a shutter having a larger one of the acquired speed parameters of the shutter 8A and shutter 8B (maximum speed parameter) on the first optical path (step S1504), and registers the selected shutter in the storage unit 25 as a high speed shutter on the first optical path (step S1505).
Next, the main controller 30 selects a shutter having a larger one of the acquired speed parameters of the shutter 8D and shutter 8F (maximum speed parameter) on the second optical path (step S1506), and registers the selected shutter in the storage unit 25 as a high speed shutter on the second optical path (step S1507).
The main controller 30 acquires operation conditions from the storage unit 25 after registering the high speed shutters (step S1508). For example, in the storage unit 25, tables of operation conditions as illustrated in
After acquiring the operation conditions, the main controller 30 outputs, based on the acquired operation conditions, control signals for operations of the shutter 8A, shutter 8B, shutter 8D, and shutter 8F to the shutter operation controller 19A, shutter operation controller 19B, shutter operation controller 19D, and shutter operation controller 19F respectively (step S1509).
The shutter operation controller 19A, shutter operation controller 19B, shutter operation controller 19D, and shutter operation controller 19F control, based on the received control signals, motors to operate the shutter 8A, shutter 8B, shutter 8D, and shutter 8F, respectively (step S1510). Thereby, the shutter 8A, shutter 8B, shutter 8D, and shutter 8F are operated in conjunction with one another.
Thereafter, the main controller 30 determines whether or not the high speed shutter on the non-selected optical path is open (step S1602). If the main controller 30 determines that the high speed shutter on the non-selected optical path is open (step S1602; Yes), by transmitting a control signal to the shutter operation controller, the main controller 30 causes this high speed shutter to close (step S1603) and proceeds to step S1604. On the contrary, if the main controller 30 determines that the high speed shutter on the non-selected optical path is not open, that is, is closed (step S1602; No), the main controller 30 proceeds to step S1604.
After performing shutter control for the non-selected optical path, the main controller 30 determines whether or not the high speed shutter on the selected optical path is closed (step S1604). If the main controller 30 determines that the high speed shutter on the selected optical path is closed (step S1604; Yes), by transmitting a control signal to the shutter operation controller, the main controller 30 causes this high speed shutter to open (step S1605) and ends the optical path selection process. On the contrary, if the main controller 30 determines that the high speed shutter on the selected optical path is not closed, that is, is open (step S1604; No), the main controller 30 ends the optical path selection process.
By the above described optical path selection process, for example, if the user has selected the first optical path and the shutter 8B and shutter 8F have been registered as the high speed shutters, as illustrated in
According to the modified example 8-1, even if there are two light sources and two shutters are connected on an optical path from each light source, by a selection of an optical path by a user, a plurality of shutters are able to be operated in conjunction with one another and shut-off of an optical path not being used in the observation becomes possible. Further, by an input of one shutter operation, a plurality of shutters are able to be operated in conjunction with one another, and release and shut-off of only an optical path being used in the observation become possible.
Further, according to the modified example 8-1, even if a user does not input a shutter selection, shutters are registered and operated so as to automatically perform an open or close operation of only a high speed shutter, an input of a selection by the user is able to be omitted and release or shut-off of an optical path become possible.
As described above, a microscope apparatus and a method of controlling an electrically driven optical unit of the microscope apparatus according to some embodiments are useful for a microscope apparatus to which optical units of the same type are connected, and in particular is suitable for an inverted microscope apparatus having a plurality of illumination optical systems.
According to some embodiments, when a plurality of electrically driven optical units of the same type is connected, it is possible to reduce time and effort required for a user to select and input an optimum operation for every electrically driven optical unit, and to improve operability for the user.
Additional advantages and modifications will readily occur to those skilled in the art. Therefore, the invention in its broader aspects is not limited to the specific details and representative embodiments shown and described herein. Accordingly, various modifications may be made without departing from the spirit or scope of the general inventive concept as defined by the appended claims and their equivalents.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2012-103967 | Apr 2012 | JP | national |
This application is a continuation of PCT international application Ser. No. PCT/JP2013/062111 filed on Apr. 24, 2013 which designates the United States, incorporated herein by reference, and which claims the benefit of priority from Japanese Patent Application No. 2012-103967, filed on Apr. 27, 2012, incorporated herein by reference.
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/JP2013/062111 | Apr 2013 | US |
| Child | 14522526 | US |
