Microspectrofluorometry of Oriented Myofibrils

Information

• NSF Award
• 8817581

Owner

• Boston Biomedical Research Institute, Inc.

• Award Id

  8817581

• Award Effective Date

  3/1/1989 - 35 years ago

• Award Expiration Date

  2/28/1993 - 31 years ago

• Award Amount

  $ 230,000.00

• Award Instrument

  Continuing grant

A fluorescence microscope coupled to a photon counting spectrofluorometer will be used to obtain fluorescence spectra, fluorescence-detected dichroism and fluorescence polarization data on labeled tropomyosin, actin and troponin incorporated into oriented muscle myofibrils and fibers. Prior work on fibers has indicated that actin subunits slightly reorient when myosin heads bind to ghost fibers not containing the regulatory proteins and when calcium binds to the regulatory proteins on the thin filament in the absence of myosin. No studies appear to have been reported of possible reorientation of actin subunits associated with myosin head binding to actin containing the regulatory proteins. This project will provide new information about subunit reorientation assoicated with regulation in systems that are close to in vivo muscle and will help to clarify the mechanism of the regulation of muscle contraction.***//

• Program Officer

  Arthur Kowalsky

• Min Amd Letter Date

  3/3/1989 - 35 years ago

• Max Amd Letter Date

  9/1/1992 - 32 years ago

• ARRA Amount

• Name

  Boston Biomedical Research Institute

• City

  Watertown

• State

  MA

• Country

  United States

• Address

  64 Grove Street

• Postal Code

  024722829

• Phone Number

  6179268040

• First Name

  Sherwin

• Last Name

  Lehrer

• Start Date

  3/1/1989 12:00:00 AM

• Name

  Life Science Biological

• Code

  61

• Name

  Health

• Code

  203000