Patent Grant
9963474
This application is a national stage under 35 U.S.C. 371 of International Application PCT/EP2014/075323, filed on Nov. 21, 2014 (currently published). International Application PCT/EP2014/075323 cites the priority of British Patent Application No. 1320636.2, filed Nov. 22, 2013 (expired).
The invention relates to compounds and pharmaceutically acceptable salts thereof comprising a dicarbonyl sequestering moiety; an optional linker moiety; and a mitochondrial targeting moiety. The invention also relates to pharmaceutical compositions containing such compounds and salts, and to the use of such compounds and salts for treating diabetes, preferably hyperglycemic diabetes. The invention also relates to the provision of a mass spectrometry probe and to a method of labelling a biological molecule for mass spectrometry detection.
Hyperglycaemia is a damaging medical condition. Hyperglycaemia wreaks its effects via inappropriate glycation of molecules within cells. This glycation occurs following generation of reactive 1,2-dicarbonyls such as glyoxal and methylglyoxal. These reactive species modify molecules in the cell and cause negative pathology.
Glycation, the non-enzymatic formation of sugar-protein and sugar-nucleotide adducts, plays a major role in disrupting cell function and causing tissue damage in a range of pathologies such as diabetes, aging and neurodegeneration [1-3]. Glycation increases in response to the elevation of glucose that occurs in unregulated diabetes and is a major cause of diabetic complications [4, 5]. Within the cell excessive glucose can lead to molecular damage through the formation of 1,2-dicarbonyl compounds such as methylglyoxal from the triose phosphate intermediates of glycolysis [1, 6] or from the metabolism of acetone generated during ketosis [7]. These reactive 1,2-dicarbonyls often exist in modified chemical forms in situ including reversible hemiacetals, hemithioacetals and hemiaminals with small biomolecules and with reactive moieties on proteins and nucleic acids [8, 9]. In addition they can react directly with free amine functions on proteins and nucleic acids, thereby generating substantial permanent modifications such as arginine-derived hydroimidazolones and lysine cross-links on proteins [10], and guanine-derived imidazopurinones on DNA [11]. Such modifications are thought to result in biochemical dysfunction by altering protein structure and activity, and by inducing genomic mutations [2]. These markers of glycation damage are elevated in many clinical samples from diabetic patients and also in animal models of diabetes and aging [2,4,9,12,13], consistent with a contribution from these reactions to cell damage and pathology. An important role for methylglyoxal and glyoxal in pathology is further supported by the existence of the glyoxalase enzyme system, which specifically degrades these two dicarbonyls [14]. Loss of the glyoxalase degradation pathway renders organisms more susceptible to glycation and subsequent damage while its over-expression increases lifespan in Caenorhabditis elegans [15]. Thus dicarbonyl-associated glycation of proteins and nucleic acids is a significant contributing factor in a range of pathologies, particularly those associated with diabetes or aging.
In hyperglycaemia, there is considerable evidence for mitochondrial damage and elevated oxidative stress that contributes to pathology, and this has been in part ascribed to mitochondrial glycation due to methylglyoxal and glyoxal [16-21]. Furthermore these reactive dicarbonyls disrupt mitochondrial function in vitro [22-24]. Therefore, understanding the contribution from glycation damage by reactive dicarbonyls to mitochondrial dysfunction is important for analyzing and understanding the pathology associated with hyperglycaemia. However, the mechanistic details are uncertain, and it has proven difficult to specifically evaluate the importance of these processes. This is in part due to the uncertainties related to the distribution of methylglyoxal and glyoxal between the cytosol and mitochondria. In known approaches to combating the effects of hyperglycaemia, it has been attempted to use reactive guanidine groups. This forms a generalised “mopping” approach. The guanidine groups react with glyoxal/methylglyoxal groups. However, this guanidine approach is entirely untargeted. This is a drawback in the art.
In addressing problem(s) associated with the prior art, the solution provided by the inventors includes compounds of the invention which specifically target molecular groups capable of sequestering the dicarbonyls that are found within mitochondria under conditions of hyperglycaemia.
These compounds couple a targeting moiety to a sequestering moiety so that the compounds preferentially accumulate in the mitochondria, where they are maximally effective in reducing and/or preventing the damage caused by the reactive dicarbonyls. Optionally, the sequestering moiety may be attached to the targeting moiety via a linker moiety. It should be noted that the approach of sequestering the reactive groups within mitochondria is new. This approach has not been contemplated in any known treatment. It should be noted that the approach underlying the invention of sequestering the reactive groups within mitochondria themselves is a departure from known techniques. These and other benefits flow from the invention as explained below.
Thus, in a broad aspect the invention relates to a compound of Formula 1:
A-L-B Formula 1
or a pharmaceutically acceptable salt thereof, wherein:
In one aspect the invention provides a pharmaceutical composition which comprises: a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, carrier or diluent.
A further aspect of the invention provides a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use as a medicament.
A further aspect of the invention provides a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the treatment of diabetes, preferably hyperglycemic diabetes.
A further aspect of the invention provides a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for the treatment of diabetes, preferably hyperglycemic diabetes.
A further aspect of the invention provides a method of treating a disease or condition in a subject, the method comprising administering to the subject an effective amount of a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, wherein the disease or condition is diabetes, preferably hyperglycemic diabetes.
A further aspect of the invention provides a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the preservation of organ and tissue for surgical transplants.
A further aspect of the invention provides a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the storage of blood.
A further aspect of the invention provides a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the prevention or treatment of hyperglycaemia.
A further aspect of the invention provides a compound of Formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use as a mass spectrometry probe.
A further aspect of the invention provides a method of labelling a biological molecule for mass spectrometry detection comprising contacting said molecules with a compound of formula 1 or a pharmaceutically acceptable salt thereof.
“Substituted”, when used in connection with a chemical substituent or moiety (e.g., an alkyl group), means that one or more hydrogen atoms of the substituent or moiety have been replaced with one or more non-hydrogen atoms or groups, provided that valence requirements are met and that a chemically stable compound results from the substitution.
“Alkyl” refers to straight chain and branched saturated hydrocarbon groups, generally having a specified number of carbon atoms (e.g., C1-3 alkyl refers to an alkyl group having 1 to 3 carbon atoms, C1-6 alkyl refers to an alkyl group having 1 to 6 carbon atoms, and so on). Examples of alkyl groups include methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, i-butyl, t-butyl, pent-1-yl, pent-2-yl, pent-3-yl, 3-methylbut-1-yl, 3-methylbut-2-yl, 2-methylbut-2-yl, 2,2,2-trimethyleth-1-yl, n-hexyl, and the like.
“Alkylene” refers to a divalent radical derived from an alkane which may be a straight chain or branched, as exemplified by —CH2CH2CH2CH2—.
The terms “cycloalkyl” by itself or in combination with other terms, represent, unless otherwise stated, a cyclic versions of “alkyl”. Examples of cycloalkyl include cyclopentyl, cyclohexyl, 1-cyclohexenyl, 3-cyclohexenyl, cycloheptyl, and the like.
The term “alkenyl”, as used herein, means a hydrocarbon radical having at least one double bond including, but not limited to, ethenyl, propenyl, 1-butenyl, 2-butenyl and the like.
The term “alkenylene” refers to a divalent radical derived from an alkenyl which may be a straight chain or branched, containing one or more double bonds, as exemplified by, —CH2CH═CH—, or —CH2CH(CH3)CH═CH—CH2—.
The term “alkynyl”, as used herein, means a hydrocarbon radical having at least one triple bond including, but not limited to, ethynyl, propynyl, 1-butynyl, 2-butynyl and the like.
The term “alkynylene” refers to a divalent unsaturated hydrocarbon group which may be linear or branched, containing one or more carbon-carbon triple bonds, as exemplified by, ethyne-1,2-diyl.
“Aryl” employed alone or in combination with other terms (e.g., aryloxy, arylthioxy, arylalkyl) refers to fully unsaturated monocyclic aromatic hydrocarbons and to polycyclic hydrocarbons having at least one aromatic ring, both monocyclic and polycyclic aryl groups generally having a specified number of carbon atoms that comprise their ring members (e.g., C6-14 aryl refers to an aryl group having 6 to 14 carbon atoms as ring members). The aryl group may be attached to a parent group or to a substrate at any ring atom and may include one or more non-hydrogen substituents unless such attachment or substitution would violate valence requirements. Examples of aryl groups include phenyl, biphenyl, cyclobutabenzenyl, indenyl, naphthalenyl, benzocycloheptenyl, biphenylenyl, fluorenyl, groups derived from cycloheptatriene cation, and the like.
“Arylene” refers to a divalent radical derived from an aryl group.
The term “alkoxy”, as used herein, means an O-alkyl group wherein “alkyl” is defined above.
The term “aralkyl” or “arylalkyl” means an aryl-alkyl-group in which the aryl and alkyl are as previously described. Preferred aralkyls comprise a lower alkyl group attached to the aryl group. Non-limiting examples of suitable aralkyl groups include phenylmethylene, 2-phenethyl and naphthalenylmethyl. The bond to the parent moiety is through the alkyl.
“Cycloalkyl” refers to saturated monocyclic and bicyclic hydrocarbon groups, generally having a specified number of carbon atoms that comprise the ring or rings (e.g., C3-8 cycloalkyl refers to a cycloalkyl group having 3 to 8 carbon atoms as ring members). Bicyclic hydrocarbon groups may include isolated rings (two rings sharing no carbon atoms), spiro rings (two rings sharing one carbon atom), fused rings (two rings sharing two carbon atoms and the bond between the two common carbon atoms), and bridged rings (two rings sharing two carbon atoms, but not a common bond). The cycloalkyl group may be attached to a parent group or to a substrate at any ring atom unless such attachment would violate valence requirements. In addition, the cycloalkyl group may include one or more non-hydrogen substituents unless such substitution would violate valence requirements.
Examples of monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like. Examples of fused bicyclic cycloalkyl groups include bicyclo[2.1.0]pentanyl (i.e., bicyclo[2.1.0]pentan-1-yl, bicyclo[2.1.0]pentan-2-yl, and bicyclo[2.1.0]pentan-5-yl), bicyclo[3.1.0]hexanyl, bicyclo[3.2.0]heptanyl, bicyclo[4.1.0]heptanyl, bicyclo[3.3.0]octanyl, bicyclo[4.2.0]octanyl, bicyclo[4.3.0]nonanyl, bicyclo[4.4.0]decanyl, and the like. Examples of bridged cycloalkyl groups include bicyclo[2.1.1]hexanyl, bicyclo[2.2.1]heptanyl, bicyclo[3.1.1]heptanyl, bicyclo[2.2.2]octanyl, bicyclo[3.2.1]octanyl, bicyclo[4.1.1]octanyl, bicyclo[3.3.1]nonanyl, bicyclo[4.2.1]nonanyl, bicyclo[3.3.2]decanyl, bicyclo[4.2.2]decanyl, bicyclo[4.3.1]decanyl, bicyclo[3.3.3]undecanyl, bicyclo[4.3.2]undecanyl, bicyclo[4.3.3]dodecanyl, and the like. Examples of spiro cycloalkyl groups include spiro[3.3]heptanyl, spiro[2.4]heptanyl, spiro[3.4]octanyl, spiro[2.5]octanyl, spiro[3.5]nonanyl, and the like. Examples of isolated bicyclic cycloalkyl groups include those derived from bi(cyclobutane), cyclobutanecyclopentane, bi(cyclopentane), cyclobutanecyclohexane, cyclopentanecyclohexane, bi(cyclohexane), etc.
“Drug”, “drug substance”, “active pharmaceutical ingredient”, and the like, refer to a compound (e.g., compounds of Formula 1 and compounds specifically named above) that may be used for treating a subject in need of treatment.
“Excipient” refers to any substance that may influence the bioavailability of a drug, but is otherwise pharmacologically inactive.
“Halo”, “halogen” and “halogeno” may be used interchangeably and refer to fluoro, chloro, bromo, and iodo. Additionally, terms such as “fluoroalkyl”, are meant to include monofluoroalkyl and polyfluoroalkyl.
“Heteroaryl” refers to unsaturated monocyclic aromatic groups and to polycyclic groups having at least one aromatic ring, each of the groups having ring atoms composed of carbon atoms and 1 to 4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. Both the monocyclic and polycyclic groups generally have a specified number of carbon atoms as ring members (e.g., C1-9 heteroaryl refers to a heteroaryl group having 1 to 9 carbon atoms and 1 to 4 heteroatoms as ring members) and may include any bicyclic group in which any of the above-listed monocyclic heterocycles are fused to a benzene ring. The heteroaryl group may be attached to a parent group or to a substrate at any ring atom and may include one or more non-hydrogen substituents unless such attachment or substitution would violate valence requirements or result in a chemically unstable compound. Examples of heteroaryl groups include monocyclic groups such as pyrrolyl (e.g., pyrrol-1-yl, pyrrol-2-yl, and pyrrol-3-yl), furanyl, thiophenyl, pyrazolyl, imidazolyl, isoxazolyl, oxazolyl, isothiazolyl, thiazolyl, 1,2,3-triazolyl, 1,3,4-triazolyl, 1-oxa-2,3-diazolyl, 1-oxa-2,4-diazolyl, 1-oxa-2,5-diazolyl, 1-oxa-3,4-diazolyl, 1-thia-2,3-diazolyl, 1-thia-2,4-diazolyl, 1-thia-2,5-diazolyl, 1-thia-3,4-diazolyl, tetrazolyl, pyridinyl, pyridazinyl, pyrimidinyl, and pyrazinyl.
Examples of heteroaryl groups also include bicyclic groups such as benzofuranyl, isobenzofuranyl, benzothiophenyl, benzo[c]thiophenyl, indolyl, 3H-indolyl, isoindolyl, 1H-isoindolyl, indolinyl, isoindolinyl, benzimidazolyl, indazolyl, benzotriazolyl, chromanyl, 2-phenylchromanyl, 3-phenylchromanyl, 4-phenylchromanyl, chromen-4-only, 2-phenylchromen-4-only, 3-phenylchromen-4-only, coumarinyl, 3-phenylcoumarinyl, 4-phenylcoumarinyl, 1,8-bis[2-chromanyl]-6-benzo[7]annuleonyl, 1H-pyrrolo[2,3-b]pyridinyl, 1H-pyrrolo[2,3-c]pyridinyl, 1H-pyrrolo[3,2-c]pyridinyl, 1H-pyrrolo[3,2-b]pyridinyl, 3H-imidazo[4,5-b]pyridinyl, 3H-imidazo[4,5-c]pyridinyl, 1H-pyrazolo[4,3-b]pyridinyl, 1H-pyrazolo[4,3-c]pyridinyl, 1H-pyrazolo[3,4-c]pyridinyl, 1H-pyrazolo[3,4-b]pyridinyl, 7H-purinyl, indolizinyl, imidazo[1,2-c]pyridinyl, imidazo[1,5-c]pyridinyl, pyrazolo[1,5-c]pyridinyl, pyrrolo[1,2-b]pyridazinyl, imidazo[1,2-c]pyrimidinyl, quinolinyl, isoquinolinyl, cinnolinyl, quinazolinyl, quinoxalinyl, phthalazinyl, 1,6-naphthyridinyl, 1,7-naphthyridinyl, 1,8-naphthyridinyl, 1,5-naphthyridinyl, 2,6-naphthyridinyl, 2,7-naphthyridinyl, pyrido[3,2-c]pyrimidinyl, pyrido[4,3-c]pyrimidinyl, pyrido[3,4-c]pyrimidinyl, pyrido[2,3-c]pyrimidinyl, pyrido[2,3-b]pyrazinyl, pyrido[3,4-b]pyrazinyl, pyrimido[5,4-c]pyrimidinyl, pyrazino[2,3-b]pyrazinyl, and pyrimido[4,5-c]pyrimidinyl.
“Heterocycle” and “heterocyclyl” may be used interchangeably and refer to saturated or partially unsaturated monocyclic or bicyclic groups having ring atoms composed of carbon atoms and 1 to 4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. Both the monocyclic and bicyclic groups generally have a specified number of carbon atoms in their ring or rings (e.g., C2-5 heterocyclyl refers to a heterocyclyl group having 2 to 5 carbon atoms and 1 to 4 heteroatoms as ring members). As with bicyclic cycloalkyl groups, bicyclic heterocyclyl groups may include isolated rings, spiro rings, fused rings, and bridged rings. The heterocyclyl group may be attached to a parent group or to a substrate at any ring atom and may include one or more non-hydrogen substituents unless such attachment or substitution would violate valence requirements or result in a chemically unstable compound. Examples of monocyclic heterocyclyl groups include oxiranyl, thiaranyl, aziridinyl (e.g., aziridin-1-yl and aziridin-2-yl), oxetanyl, thiatanyl, azetidinyl, tetrahydrofuranyl, tetrahydrothiophenyl, pyrrolidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, 1,4-dioxanyl, 1,4-oxathianyl, morpholinyl, 1,4-dithianyl, piperazinyl, 1,4-azathianyl, oxepanyl, thiepanyl, azepanyl, 1,4-dioxepanyl, 1,4-oxathiepanyl, 1,4-oxaazepanyl, 1,4-dithiepanyl, 1,4-thiazepanyl, 1,4-diazepanyl, 3,4-dihydro-2H-pyranyl, 5,6-dihydro-2H-pyranyl, 2H-pyranyl, 1,2,3,4-tetrahydropyridinyl, and 1,2,5,6-tetrahydropyridinyl
“Pharmaceutically acceptable” substances refers to those substances which are within the scope of sound medical judgment suitable for use in contact with the tissues of subjects without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit-to-risk ratio, and effective for their intended use.
“Pharmaceutical composition” refers to the combination of one or more drug substances and one or more excipients.
The term “sulfonyl” refers to a radical —S(O)2R where R is an alkyl, substituted alkyl, substituted cycloalkyl, substituted heterocycloalkyl, substituted aryl, or substituted heteroaryl group as defined herein. Representative examples include, but are not limited to methylsulfonyl, ethylsulfonyl, propylsulfonyl, butylsulfonyl, and the like.
The term “sulfinyl” refers to a radical —S(O)R where R is an alkyl, substituted alkyl, substituted cycloalkyl, substituted heterocycloalkyl, substituted aryl, or substituted heteroaryl group as defined herein. Representative examples include, but are not limited to, methylsulfinyl, ethylsulfinyl, propylsulfinyl, butylsulfinyl, and the like.
The term “subject” as used herein refers to a human or non-human mammal. Examples of non-human mammals include livestock animals such as sheep, horses, cows, pigs, goats, rabbits, deer, ostriches and emus; and companion animals such as cats, dogs, rodents, and horses.
“Therapeutically effective amount” of a drug refers to the quantity of the drug that may be used for treating a subject and may depend on the weight and age of the subject and the route of administration, among other things.
“Treating” refers to reversing, alleviating, inhibiting the progress of, or preventing a disorder, disease or condition to which such term applies, or to reversing, alleviating, inhibiting the progress of, or preventing one or more symptoms of such disorder, disease or condition.
“Treatment” refers to the act of “treating”, as defined immediately above.
As used herein the term “comprising” means “consisting at least in part of”. When interpreting each statement in this specification that includes the term “comprising”, features other than that or those prefaced by the term may also be present. Related terms such as “comprise” and “comprises” are to be interpreted in the same manner.
Dicarbonyl Sequestering Moiety
The dicarbonyl sequestering moiety A comprises a substituted aryl group or a substituted heteroaryl group. A diacarbonyl sequestering moiety is a moiety that forms a stable compound, such as a quinoxaline compound, with a dicarbonyl molecule, suitably, with a 1,2-dicarbonyl molecule. The dicarbonyl sequestering moiety is used to sequester dicarbonyl molecules in biological systems, such as the dilute solutions found in cells. In order for the dicarbonyl sequestering moiety to form a stable compound with a dicarbonyl molecule under the conditions found in cells, the dicarbonyl sequestering moiety must be sufficiently nucleophilic to form adducts spontaneously with the dicarbonyl molecule at low concentrations in water at body temperature. In the dilute solutions of biological systems, such as those found in cells, the first step to produce a stable compound with a dicarbonyl compound is to form a hemiacetal, hemiaminal or hemithioacetal which is a reversible reaction with the equilibrium in favour of the starting materials.
Suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —NH2, —NHR1, —NR1R1, —1X—NH2, —1X—NHR1, —O—NH2, —O—NHR1, —1X—O—NH2, —1X—O—NHR1, —NR′—NHR′, —1X—NR′—NHR′ and —NHCOR1.
Suitably the substituted aryl group or substituted heteroaryl group comprises from two to nine substituent groups. More suitably, the substituted aryl group or substituted heteroaryl group comprises from two to eight substituent groups; from two to seven substituent groups; from two to six substituent groups; or from two to five substituent groups.
Suitably the substituted aryl group or substituted heteroaryl group comprises from two to nine substituent groups independently selected from —OH, —OR1, —NH2, —NHR1, —NR1R1, —1X—NH2, —1X—NHR1, —O—NH2, —O—NHR1, —1X—O—NH2, —1X—O—NHR1, —NR′—NHR′, —1X—NR′—NHR′, —NHCOR1 and —O—C(O)—R1.
Thus, the substituent groups may comprise hydrazine derivatives such as —NH—NH2, —NH—NH(C1-6 alkyl), —N(C1-6 alkyl)-NH2, —N(C1-6 alkyl)-NH(C1-6 alkyl), —1X—NH—NH2, —1X—NH—NH(C1-6 alkyl), —1X—N(C1-6 alkyl)-NH2 and —1X—N(C1-6 alkyl)-NH(C1-6 alkyl).
Suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —OH, —OR1, —NH2, —NHR1, —NR1R1, —1X—NH2, —1X—NHR1, —NHCOR1, and —O—C(O)—R1.
Suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —NH2, —NHR1, —NR1R1, —1X—NH2, —1X—NHR1 and —NHCOR1.
Suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —OH, —OR1, —NH2, —NHR1, —NR1R1, —C1-6 alkylene-NH2, —C1-6 alkylene-NHR1, —NHCOR1, and —O—C(O)—R1.
More suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —OH, —OR1, —NH2 and —C1-6 alkylene-NH2.
More suitably the substituted aryl group, or the substituted heteroaryl group comprises two or more substituent groups independently selected from —NH2 and —C1-6 alkylene-NH2.
Suitably the substituted aryl group, or the substituted heteroaryl group comprises from one to seven of the optional substituent groups; from one to six of the optional substituent groups; from one to five of the optional substituent groups; from one to four of the optional substituent groups; from one to three of the optional substituent groups.
Suitably, the substituted aryl group, or the substituted heteroaryl group comprises one or more of the optional substituent groups selected from —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, -halogen, —1X—OH, —1X—O—R1, —CO2H, —1X—CO2H, —CO2R1, —1X—CO2R1, —C(O)NH1, —C(O)NHR1, —SO2NH2 and —SO2NHR1.
Suitably, the substituted aryl group, or the substituted heteroaryl group comprises one or more of the optional substituent groups selected from —C1-6 alkyl, -halogen, —C1-6 alkylene-OH, —C1-6 alkylene-O—R1, —CO2H, —C1-6 alkylene-CO2H, —CO2R1, —C1-6 alkylene-CO2R1, —C1-6 alkylene-O—C(O)—R1, —CHO and —C(O)—R1, —C(O)NH2, —C(O)NHR1, —SO2NH2 and —SO2NHR1.
Suitably, the substituted aryl group, or the substituted heteroaryl group comprises one or more of the optional substituent groups selected from —C1-6 alkyl, -halogen, —C1-6 alkylene-OH, —C1-6 alkylene-O—R1, —CO2H, —CO2R1.
More suitably, R1 is a —C1-6 alkyl. More suitably, R1 is selected from methyl, ethyl, n-propy, i-propyl, n-butyl, s-butyl, i-butyl and t-butyl.
More suitably, R1 has the structure of Formula 2:
More suitably, two or more of R2-R6 are —H.
More suitably, R2 and R6 are —H.
More suitably, R2 and R6 are —H and R3, R4 and R5 are —OH
Suitably, A is a dicarbonyl sequestering moiety comprising a substituted aryl group selected from substituted phenyl, biphenyl and naphthalenyl; or a substituted heteroaryl group selected from substituted pyrrolyl, furanyl, thiophenyl, pyrazolyl, imidazolyl, pyridinyl, pyridazinyl, pyrimidinyl, chromanyl, 2-phenylchromanyl, 3-phenylchromanyl, 4-phenylchromanyl, chromen-4-only, 2-phenylchromen-4-only, 3-phenylchromen-4-only, coumarinyl, 3-phenylcoumarinyl, 4-phenylcoumarinyl and 1,8-bis[2-chromanyl]-6-benzo [7]annuleonyl.
More suitably, A is a dicarbonyl sequestering moiety comprising a substituted aryl group selected from substituted phenyl and naphthalenyl; or a substituted heteroaryl group selected from substituted pyridinyl, chromanyl, 2-phenylchromanyl, 3-phenylchromanyl, 4-phenylchromanyl, chromen-4-only, 2-phenylchromen-4-only, 3-phenylchromen-4-only, coumarinyl, 3-phenylcoumarinyl, 4-phenylcoumarinyl and 1,8-bis[2-chromanyl]-6-benzo [7]annuleonyl.
More suitably, A is a dicarbonyl sequestering moiety comprising a substituted aryl group selected from substituted phenyl and naphthalenyl; or a substituted heteroaryl group selected from substituted pyridinyl.
More suitably, the dicarbonyl sequestering moiety A is a substituted phenyl.
More suitably, the dicarbonyl sequestering moiety A is a substituted phenyl comprising from two to five substituent groups; more suitably, from two to four substituent groups; more suitably, from two to three substituent groups.
More suitably, the dicarbonyl sequestering moiety A is a substituted aryl group comprising Formula 3:
wherein two or more of R7-R11 are independently selected from —OH, —OR1, —NH2, —NHR1, —NR1R1, —C1-6 alkylene-NH2, —C1-6 alkylene-NHR1, —O—NH2, —O—NHR1, —C1-6 alkylene-O—NH2, —C1-6 alkylene-O—NHR1, —NHCOR1, —O—C(O)—R1, —NR′—NHR′, —C1-6 alkylene —NR′—NHR′; and
the remaining groups R7-R11 are independently selected from —H, —C1-6 alkyl, —C2-6 alkenyl, —C2-6 alkynyl, -halogen, —C1-6 alkylene-OH, —C1-6 alkylene-O—R1, —CO2H, —C1-6 alkylene-CO2H, —CO2R1, —C1-6 alkylene-CO2R1, —C1-6 alkylene-O—C(O)—R1, —CH(OH)—C(O)—R1, —C1-6 alkylene-CH(OH)—C(O)—R1, —CHO, —C(O)—R1, —C(O)NH2, —C(O)NHR1, —SO2NH2 and —SO2NHR1.
More suitably two or more of R7-R11 are independently selected from —OH, —NH2, —OC1-C6 alkyl, —NH(C1-C6 alkyl), —C1-C6 alkylene-NH2, —C1-C6 alkylene-NH(C1-C6 alkyl).
More suitably two or more of R7-R11 are independently selected from —OH, —OC1-C6 alkyl, —NH2, —NH(C1-C6 alkyl), —C1-C6 alkylene-NH2 and —C1-C6 alkylene-NH(C1-C6 alkyl); and the remaining groups R7-R11 are independently selected from —H, —C1-6 alkyl, —C1-6 alkylene-OH, —C1-6 alkylene-O—C1-6 alkyl, —CO2H, —CO2C1-6 alkyl, —CH(OH)—C(O)—C1-6 alkyl and —CHO.
Suitably, from one to three of R7-R11 are —H.
More suitably two or more of R7-R11 is independently selected from —OH, —OC1-C6 alkyl, —NH2, —NH(C1-C6 alkyl), —C1-C6 alkylene-NH2 and —C1-C6 alkylene-NH(C1-C6 alkyl); and the remaining groups R7-R11 are independently selected from —H, —C1-6 alkyl, —C1-6 alkylene-OH and —C1-6 alkylene-OC1-6 alkyl.
More suitably, R7=—H, R10=—H, and R11=—H, and R8 and R9 are independently selected from —OH, —OR1, —NH2, —NHR1, —C1-6 alkylene-NH2, —C1-6 alkylene-NHR1, —O—NH2, —O—NHR1, —C1-6 alkylene-O—NH2, —C1-6 alkylene-O—NHR1, —NHCOR1 and —O—C(O)—R1.
More suitably, R7=—H, R10=—H, and R11=—H, and R8 and R9 are independently selected from OH, —OC1-C6 alkyl, —NH2, —NH(C1-C6 alkyl), —C1-C6 alkylene-NH2 and —C1-C6 alkylene-NH(C1-C6 alkyl).
More suitably, R7=—H, R10=—H, and R11=—H, and R8 and R9 are independently selected from —OH, —C1-C6 alkylene-OH, —NH2 and —C1-C6 alkylene-NH2.
More suitably, R7=—H, R8=—NH2, R9=—NH2, R10=—H, and R11=—H. This results in a dicarbonyl sequestering moiety A of Formula 4:
In one aspect, the dicarbonyl sequestering moiety A comprises a substituted flavonoid or theaflavin compound which comprises a heteroaryl group selected from substituted chromanyl, 2-phenylchromanyl, 3-phenylchromanyl, 4-phenylchromanyl, chromen-4-only, 2-phenylchromen-4-only, 3-phenylchromen-4-only, coumarinyl, 3-phenylcoumarinyl, 4-phenylcoumarinyl and 1,8-bis[2-chromanyl]-6-benzo[7]annuleonyl.
The trapping of species such as glyoxal and methylglyoxal with various flavonoid or theaflavin compounds have been described [55]-[57].
More suitably, the substituted flavonoid or theaflavin compound comprises one or more substituent groups independently selected from —OH, —OR1, —NHCOR1 and —O—C(O)—R1; and may optionally comprises one or more substituent groups selected from —C1-6 alkyl, -halogen, —C1-6 alkylene-OH, —C1-6 alkylene-O—R1, —CO2H, —CO2R1, —CH(OH)—C(O)—R1 and —CHO, and
More suitably, the substituted flavonoid compound comprises one or more substituent groups independently selected from —OH, —OR1 and —O—C(O)—R1; and may optionally comprises one or more substituent groups selected from —C1-6 alkyl, —C1-6 alkylene-OH, —C1-6 alkylene-O—R1, —CO2H and —CO2R1, and
In some aspects, A is a dicarbonyl sequestering moiety that does not include a benzoquinone moiety.
Suitably, 1X is selected from C1-6 alkylene and C2-6 alkenylene.
More suitably, 1X is a C1-6 alkylene.
The ability of a diacarbonyl sequestering moiety to form a stable compound with a dicarbonyl molecule, such as methylglyoxal, could be tested by carrying out a competition assay with a detection system [64] where the methylglyoxal produces a fluorescent product that can be detected by HPLC.
Linker Moiety
The linker moiety -L- is divalent and may be any chemically non-active distance-making group (spacer) which joins the mitochondrial targeting moiety to the dicarbonyl sequestering moiety, and enables the two moieties to remain bonded together when crossing the plasma and mitochondrial membranes. In particular, -L- is stable under physiological conditions and must be pharmaceutically acceptable.
Suitably -L- is a linker moiety of Formula 5:
—(Z1)m—X1—Yn—[X2]s—(Z2)t— Formula 5
wherein:
Z1 and Z2 are independently selected from O, NR12, NR12—C(O), C(O)NR12, O—C(O), C(O)—O and S;
Y is selected from O, NR12, NR12—C(O), C(O)NR12, O—C(O), C(O)—O, S and arylene;
wherein R12 is selected from —H, —C1-C6 alkyl and -aryl;
X1 is selected from C1-Cp alkylene, C2-Cp alkenylene, C2-Cp alkynylene and C3-Cp cycloalkylene;
X2 is selected from C1-Cq alkylene, C2-Cq alkenylene, C2-Cq alkynylene and C3-Cq cycloalkylene;
each of m, n, s and t is independently selected from 0 or 1;
wherein p+q=30 and wherein X1 and X2 are optionally substituted with one or more functional groups independently selected from the group consisting of hydrogen, alkyl, cycloalkyl, alkenyl, alkynyl, haloalkyl, aryl, aminoalkyl, hydroxyalkyl, alkoxyalkyl, alkylthio, alkylsulfinyl, alkylsulfonyl, carboxyalkyl, cyano, oxy, amino, alkylamino, aminocarbonyl, alkoxycarbonyl, aryloxycarbonyl, alkylaminocarbonyl, arylaminocarbonyl, aralkylaminocarbonyl, alkylcarbonylamino, arylcarbonylamino, aralkylcarbonylamino, alkylcarbonyl, heterocyclocarbonyl, aminosulfonyl, alkylaminosulfonyl, alkylsulfonyl, and heterocyclosulfonyl, or the substituent groups of adjacent carbon atoms in the linker group can be taken together with the carbon atoms to which they are attached to form a carbocycle or a heterocycle.
Suitably, Z1 is adjacent to the dicarbonyl sequestering moiety and Z2 is adjacent to the mitochondrial targeting moiety.
More suitably, X1 is substituted with one or more functional groups independently selected from the group consisting of alkyl, aryl, alkoxycarbonyl, aryloxycarbonyl, alkylaminocarbonyl, arylaminocarbonyl, alkylcarbonylamino and arylcarbonylamino.
More suitably, X2 is substituted with one or more functional groups independently selected from the group consisting of alkyl, aryl, alkoxycarbonyl, aryloxycarbonyl, alkylaminocarbonyl, arylaminocarbonyl, alkylcarbonylamino and arylcarbonylamino.
More suitably, the linker moiety is of Formula 5, wherein:
Z1 and Z2 are as described above;
X1 and X2 are as described above;
Y is selected from NR12—C(O), C(O)NR12 and O—C(O);
R12 is as described above;
p=12;
q=5;
m, n, s and t are as described above; and
X1 and X2 are optionally substituted with one or more functional groups independently selected from the group consisting alkyl, aryl, alkoxycarbonyl, aryloxycarbonyl, alkylaminocarbonyl, arylaminocarbonyl, alkylcarbonylamino and arylcarbonylamino.
More suitably, X1 is selected from C1-Cp alkylene.
More suitably, X2 is selected from C1-Cp alkylene.
More suitably, m is 1.
More suitably, -L- is a linker moiety of Formula 6:
—(Z1)m—X1—(Z2)t— Formula 6
wherein Z1, m, Z2 and t have the same meanings as defined above, X1 is selected from C1-Cp alkylene, C2-Cp alkenylene, C2-Cp alkynylene and C3-Cp cycloalkylene and Cp=30.
More suitably, -L- is a linker moiety of Formula 6 and Z1 and Z2 are independently selected from O, NR12, NR12—C(═O) and C(═O)NR12.
More suitably, X1 is selected from C1-Cp alkylene.
More suitably, -L- is a linker moiety of Formula 6, wherein Cp=25; more suitably, Cp=20; more suitably, Cp=15; more suitably, Cp=12; more suitably, Cp=10; more suitably, Cp=9; more suitably, Cp=8; more suitably, Cp=7; more suitably, Cp=6.
More suitably -L- is a linker moiety of Formula 7:
—(Z1)m—(C1-Cp)alkylene- Formula 7
wherein Z1 and m have the same meanings as defined above and Cp=30.
Suitably, Z1 is adjacent to the dicarbonyl sequestering moiety and the C1-Cp alkylene is adjacent to the mitochondrial targeting moiety.
More suitably, -L- is a linker moiety of Formula 7 and m is 1 and Z1 is selected from O and C(O)NR12.
More suitably, -L- is a linker moiety of Formula 7 and R12 is selected from —H and —C1-C6 alkyl.
More suitably, -L- is a linker moiety of Formula 7 and m is 1 and Z1 is C(O)NH.
More suitably, -L- is a linker moiety of Formula 7 wherein Cp=25; more suitably, Cp=20; more suitably, Cp=15; more suitably, Cp=10; more suitably, Cp=9; more suitably, Cp=8; more suitably, Cp=7; more suitably, Cp=6
More suitably, -L- is a linker moiety of Formula 7 and the C1-Cp alkylene is a C3-C6 alkylene.
More suitably, -L- is a linker moiety of Formula 7, wherein m is 1, Z1 is selected from O and C(O)NR12; and the C1-Cp alkylene is selected from a C3-C6 alkylene.
More suitably, -L- is a linker moiety of Formula 7, wherein m is 1, Z1 is C(O)NH; and the C1-Cp alkylene is selected from a C3-C6 alkylene.
In one aspect, -L- is a linker moiety of Formula 8:
wherein R13 is a C1-C6 alkylene, and R14 is a C1-C6 alkylene.
In one aspect, -L- is a linker moiety of Formula 9:
wherein R13 is a C1-C6 alkylene, and R14 is a C1-C6 alkylene.
More suitably, for Formula 8 or 9 R13 is —CH2—CH2—CH2—.
More suitably, for Formula 8 or 9 R14 is —CH2—CH2—CH2—.
Linker moieties of Formula 8 or 9 are described in US 2009/0099080.
Mitochondrial Targeting Moiety
Many mitochondrial targeting moieties are known in the art. Compounds comprising mitochondrial targeting moieties accumulate in high concentrations within mitochondria following administration.
The accumulation of compounds comprising mitochondrial targeting moieties within mitochondria may, for example, be driven by plasma membrane potential and/or mitochondrial membrane potentials. Such accumulation can often be adequately described by the Nernst equation and, for example, for compounds comprising a triphenylphosphonium cation may be 10-fold per 60 mV membrane potential under typical biological conditions [25-27]. As a result, compounds comprising a mitochondrial targeting moiety, such as the triphenylphosphonium cation, may accumulate several hundred-fold or more within mitochondria in vivo assuming plasma and mitochondrial membrane potentials of 30 mV and 160 mV respectively [28, 29].
Suitably, the mitochondrial targeting moiety B is a cationic mitochondrial targeting moiety or a mitochondrial targeting peptide.
A range of mitochondrial targeting peptides are known in the art. Suitable examples are disclosed in US 2009/0099080.
Suitably, the mitochondrial targeting moiety B is a cationic mitochondrial targeting moiety. A cationic mitochondrial targeting moiety will also be associated with a pharmaceutically acceptable anion.
Suitably, the anion is selected from acetate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, bisulfate, sulfate, methylsulfonate, nitrate, phosphate, hydrogen phosphate, dihydrogen phosphate and trifluoroacetate salts.
Suitably, the mitochondrial targeting moiety B is a lipophilic cation.
Liphophilic cations are suitable mitochondrial targeting moieties because they can readily pass directly through phospholipid bilayers without having to overcome large energy barriers or requiring a specific uptake mechanism, and they accumulate substantially within mitochondria due to the large membrane potential.
Without wishing to be bound to one theory, it is believed that lipophilic cations are taken up from a positively charged cellular compartment into a negatively charged compartment until a sufficiently large concentration gradient is built up to equalize the electrochemical potential of the molecules in the two compartments. For every 60 mV increase in membrane potential, there will be approximately tenfold accumulation of the lipophilic cation within mitochondria. Because the plasma membrane has a negative 30-60 mV potential on the inside, lipophilic cations will accumulate 5 to 10 fold in the cytosol. Lipophilic cations within the cytosol will accumulate in mitochondria because the mitochondrial membrane potential is typically about 140 to 180 mV.
Suitably, the mitochondrial targeting moiety B is selected from:
(i) cationic mitochondrial targeting moieties comprising a quaternary ammonium or phosphonium cation;
(ii) cationic mitochondrial targeting moieties comprising a 1,4a,8-triaza-2,3,4,5,6,7-hexahydro-1H-napthalene compound; and
(iii) cationic mitochondrial targeting moieties comprising a Rhodamine compound.
More suitably B is (i) a cationic mitochondrial targeting moiety comprising a quaternary ammonium or phosphonium cation of Formula 10:
wherein:
More suitably D is phosphorous.
More suitably, each of R15, R16 and R17 is independently selected from substituted or unsubstituted alkyl, benzyl, phenyl, naphthyl, furanyl, pyridyl and thiophenyl.
More suitably, where any of R15, R16 and R17 are substituted, the substituted alkyl, benzyl, aryl or heteroaryl are substituted with from 1 to 3 substitutents selected from the group consisting of -halogen, —OH, —SH, —OC1-6 alkyl, —SC1-6 alkyl, —SPh, —C1-6 alkyl, —C2-6 alkenyl, —C1-6 alkynyl, —C1-6 hydroxyalkyl, —C1-6 aminoalkyl, —C6-18 aralkyl, —C6-12 aryl, —C3-8 cycloalkyl, —C1-12 heteroaryl and —C1-12 heterocyclyl.
Suitably, where one or more of R15, R16 and R17 is independently selected from substituted or unsubstituted alkyl, each alkyl group is independently selected from a substituted or unsubstituted C1-30 alkyl. More suitably, each alkyl group is independently selected from a substituted or unsubstituted C1-25 alkyl; from a substituted or unsubstituted C1-20 alkyl; from a substituted or unsubstituted C1-15 alkyl; from a substituted or unsubstituted C1-10 alkyl.
More suitably each of R15, R16 and R17 are the same.
More suitably each R15, R16 and R17 is an unsubstituted or a substituted aryl group.
More suitably each R15, R16 and R17 is an unsubstituted aryl group.
More suitably each aryl group is phenyl.
Most suitably, B is a triphenylphosphonium (TPP) cation, which has the Formula 11:
The large hydrophobic radius of the TPP cation enables it to pass easily through the phospholipid bilayer relative to other cations.
Lipophilic triphenylphosphonium (TPP) cation functionality is suitable to target mitochondria and it has been shown to direct a wide variety of antioxidants, probes and bioactive molecules to mitochondria in cells, animal models and patients following intravenous, oral or intraperitoneal administration [25-27]. Uptake occurs directly through the phospholipid bilayer and does not require a protein carrier, while the extent of accumulation into mitochondria is determined by the membrane potential.
More suitably, B is (ii) a cationic mitochondrial targeting moiety comprising 1,4a,8-triaza-2,3,4,5,6,7-hexahydro-1H-napthalene compound of Formula 12
wherein R18 is —H, —C1-C6 alkyl, —C1-C6 alkenyl, —C1-C6 alkynyl, —C1-C6 alkylene-halogen, -aryl, -aryl-C1-C6alkyl or R19R20R21Si wherein R19, R20 and R21 are independently selected from —C1-C6 alkyl and -aryl; and v is 1, 2 or 3. Such cationic mitochondrial targeting moieties are described in US 2009/099080.
As disclosed in US 2009/099080, linker moieties of Formula 9 are suitable for use with cationic mitochondrial targeting moieties of Formula 12
More suitably, B is (iii) a cationic mitochondrial targeting moiety comprising a Rhodamine compound of Formula 13:
wherein R22, R23 and R24 are independently selected from —H and —C1-C6 alkyl;
R26 and R27 are independently selected from —H or —CH3;
R28 is selected from —CO2R29, —O—C(O)—R29, —C(O)—NHR29 and —NH—C(O)—R29; and
one of R25 and R29 is a bond to the linker L and the other of R25 and R28 is selected from —H and —C1-C6 alkyl.
A large number of Rhodamine derivatives such as Rhodamine 123, Rhodamine B, Rhodamine 6G, Rhodamine 19, Rhodamine 110, and Rhodamine 116 are commercially available from suppliers such as Acos Organics, Aldrich and Fluka. The synthesis of Rhodamine derivatives has also been reviewed [54].
Further Compounds of Formula 1
Suitably, the compound or pharmaceutically acceptable salt of Formula 1 is a salt of Formula 14:
wherein R7, R8, R9, R10, R11, R15, R16, R17, Z1, X1, Y, X2, Z2, D, m, n, s and t have the same meanings as described above. The salt of Formula 14 may optionally further comprise a pharmaceutically acceptable anion.
More suitably, the compound or pharmaceutically acceptable salt of Formula 1 is a salt of Formula 15:
wherein R7, R8, R9, R10, R11, R15, R16, R17, Z1, X1, Z2, m and t have the same meanings as described above. The salt of Formula 15 may optionally further comprise a pharmaceutically acceptable anion.
More suitably, the compound of Formula 1 is MitoG which has the structure shown in Formula 16:
wherein An− represents an optional pharmaceutically acceptable anion.
Another example of a compound of Formula 1 is MitoGamide, (3-(3,4-diaminobenoylamino)propyl)triphenylphosphonium salt, which has the structure shown in Formula 17:
wherein An− represents an optional pharmaceutically acceptable anion.
Suitably, the anion An− is selected from acetate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, bisulfate, sulfate, methylsulfonate, nitrate, phosphate, hydrogen phosphate, dihydrogen phosphate and trifluoroacetate salts.
More suitably, the anion An− is the chloride salt.
These molecules comprise a mitochondrial targeting group, such as a triphenylphosphonium ion (TPP), linked through an oxygen atom to a sequestering group, such as an o-phenylenediamine, that reacts selectively with methylglyoxal and glyoxal. Alkoxy-substituted phenylenediamines have been used for detecting 1,2-dicarbonyls because of the enhanced reactivity due to the electron donating alkoxy substituent [28, 29]. These have been as used derivatizing agents for the detection of dicarbonyls, reacting to form stable quinoxaline products [12, 13, 28].
Compounds such as MitoG are demonstrated to be protective, as shown in the examples section.
Compounds such as MitoGamide are also protective and show increased stability.
In designing the MitoG compounds such as MitoGamides, the characteristics required to bring about the therapeutic benefits of the invention were considered. A need to pull electrons into a separate part of the molecular structure was identified. The inventors identified molecular groups which could be substituted in order to tune the reactivity of the diamine group. Solutions to this problem are provided by the compounds of Formula 1. Preferred solutions include the MitoGamide molecule which provides benefits including enhanced stability.
Compounds of Formula 1, which include compounds specifically named above, may form pharmaceutically acceptable salts. These salts include acid addition salts (including di-acids) and base salts. Pharmaceutically acceptable acid addition salts include nontoxic salts derived from inorganic acids such as hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, hydrobromic acid, hydroiodic acid, hydrofluoric acid, and phosphorous acids, as well nontoxic salts derived from organic acids, such as aliphatic mono- and dicarboxylic acids, phenyl-substituted alkanoic acids, hydroxy alkanoic acids, alkanedioic acids, aromatic acids, aliphatic and aromatic sulfonic acids, etc. Such salts include acetate, adipate, aspartate, benzoate, besylate, bicarbonate, carbonate, bisulfate, sulfate, borate, camsylate, citrate, cyclamate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mesylate, methylsulfonate, naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, pyroglutamate, saccharate, stearate, succinate, tannate, tartrate, tosylate, trifluoroacetate and xinofoate salts.
Pharmaceutically acceptable base salts include nontoxic salts derived from bases, including metal cations, such as an alkali or alkaline earth metal cation, as well as amines. Examples of suitable metal cations include sodium (Na+) potassium (K+), magnesium (Mg2+), calcium (Ca2+), zinc (Zn2+), and aluminum (Al3+). Examples of suitable amines include arginine, N,N′-dibenzylethylenediamine, chloroprocaine, choline, diethylamine, diethanolamine, dicyclohexylamine, ethylenediamine, glycine, lysine, N-methylglucamine, olamine, 2-amino-2-hydroxymethyl-propane-1,3-diol, and procaine. For a discussion of useful acid addition and base salts, see S. M. Berge et al., J. Pharm. Sci. (1977) 66:1-19; see also Stahl and Wermuth, Handbook of Pharmaceutical Salts: Properties, Selection, and Use (2002).
Pharmaceutically acceptable salts may be prepared using various methods. For example, one may react a compound of Formula 1 with an appropriate acid or base to give the desired salt. One may also react a precursor of the compound of Formula 1 with an acid or base to remove an acid- or base-labile protecting group or to open a lactone or lactam group of the precursor. Additionally, one may convert a salt of the compound of Formula 1 to another salt through treatment with an appropriate acid or base or through contact with an ion exchange resin. Following reaction, one may then isolate the salt by filtration if it precipitates from solution, or by evaporation to recover the salt. The degree of ionization of the salt may vary from completely ionized to almost non-ionized.
Compounds of Formula 1 may also exist in unsolvated and solvated forms. The term “solvate” describes a molecular complex comprising the compound and one or more pharmaceutically acceptable solvent molecules (e.g., EtOH). The term “hydrate” is a solvate in which the solvent is water. Pharmaceutically acceptable solvates include those in which the solvent may be isotopically substituted (e.g., D2O, acetone-d6, DMSO-d6).
A currently accepted classification system for solvates and hydrates of organic compounds is one that distinguishes between isolated site, channel, and metal-ion coordinated solvates and hydrates. See, e.g., K. R. Morris (H. G. Brittain ed.) Polymorphism in Pharmaceutical Solids (1995). Isolated site solvates and hydrates are ones in which the solvent (e.g., water) molecules are isolated from direct contact with each other by intervening molecules of the organic compound. In channel solvates, the solvent molecules lie in lattice channels where they are next to other solvent molecules. In metal-ion coordinated solvates, the solvent molecules are bonded to the metal ion.
When the solvent or water is tightly bound, the complex will have a well-defined stoichiometry independent of humidity. When, however, the solvent or water is weakly bound, as in channel solvates and in hygroscopic compounds, the water or solvent content will depend on humidity and drying conditions. In such cases, non-stoichiometry will typically be observed.
Compounds of Formula 1 may also exist as multi-component complexes (other than salts and solvates) in which the compound (drug) and at least one other component are present in stoichiometric or non-stoichiometric amounts. Complexes of this type include clathrates (drug-host inclusion complexes) and co-crystals. The latter are typically defined as crystalline complexes of neutral molecular constituents which are bound together through non-covalent interactions, but could also be a complex of a neutral molecule with a salt. Co-crystals may be prepared by melt crystallization, by recrystallization from solvents, or by physically grinding the components together. See, e.g., O. Almarsson and M. J. Zaworotko, Chem. Commun. (2004) 17:1889-1896. For a general review of multi-component complexes, see J. K. Haleblian, J. Pharm. Sci. (1975) 64(8):1269-88.
Synthetic Methods
Methods for the chemical synthesis of compounds of the present invention are described herein. These methods may be modified and/or adapted in known ways in order to facilitate the synthesis of additional compounds within the scope of the present invention. The amounts of reactants given are for guidance. Descriptions of general laboratory methods and procedures, useful for the preparation of the compounds of the present invention, are described in Vogel's Textbook of Practical Organic Chemistry (5th edition, Ed. Furniss, B. S., Hannaford, A. J., Smith, P. W. G., Tatchell, A. R., Longmann, UK).
The synthesis of compounds of the present invention may have three key steps:
(i) formation of the dicarbonyl sequestering moiety;
(ii) attachment of the linking group to the dicarbonyl sequestering moiety; and
(iii) attachment of the linking group to the mitochondrial targeting moiety.
In some cases, the starting material for formation of the dicarbonyl sequestering moiety may already include part or all of the linking group. Where all of the linking group is included, then step (ii) is omitted.
These three steps can be carried out in any order, which will be dependent on the methods used and the nature of each of the three groups. It is possible that the formation of the dicarbonyl sequestering moiety can be interrupted by linking a precursor to the linking group. If necessary, protecting groups can be employed to avoid any unwanted reactions occurring during the synthesis.
Formation of the dicarbonyl sequestering moiety: this will depend on the nature of the dicarbonyl sequestering moiety, and can usually be based on the disclosed routes for forming that moiety. It is sometimes convenient to synthesise the dicarbonyl sequestering moiety with a heteroatom (O, S or NH) already attached to the dicarbonyl sequestering moiety in the position where this moiety is desired to be attached to the linker moiety. Such a heteroatom can aid the joining of the linking group to the dicarbonyl sequestering moiety. The dicarbonyl sequestering moiety may also be synthesised with a functional group present, such as a carboxylic acid, which is suitable for further functionalization.
Linking the linking group to the mitochondrial targeting moiety: it is generally preferred to carry this step out by heating an halogenated precursor, preferably an iodinated or brominated precursor (RBr or RI), or a precursor with a strong leaving group, such as a mesylate, sometimes in an appropriate solvent with 2-3 equivalents of the mitochondrial targeting moiety precursor under argon for up to several days. R can either be the linking group, the linking group already attached to the dicarbonyl sequestering moiety, or the linking group attached to a precursor of the dicarbonyl sequestering moiety. Where an halogenated precursor is used in the reaction, the product compound is then isolated as its bromide or iodide salt. To do this the solvent is removed (if necessary), the product is then triturated repeatedly with a compound such as diethyl ether, until an solid remains. This can then dissolved in a solvent, e.g. dichloromethane, and precipitated with diethyl ether to remove the excess unreacted material. This can be repeated. Purification can involve recrystallisation, for example, from methylene chloride/diethyl ether or chromatography on silica gel eluting with dichloromethane/ethanol mixtures.
Linking the linking group to the dicarbonyl sequestering moiety: this will depend on the nature of the dicarbonyl sequestering moiety. One method of achieving this linking is to synthesise the linking group as part of the dicarbonyl sequestering moiety. In this case, the linking group would suitably have a functional group at the end of the linking group that is to be attached to the mitochondrial targeting moiety. Such a functional group may, for example, be a good leaving group such as a mesylate group which could then be reacted with the precursor of the mitochondrial targeting moiety. Alternatively, if the dicarbonyl sequestering moiety has been synthesised with a suitable functional group in place (see above), it may be reacted with a combined mitochondrial targeting-linker precursor moiety using a coupling agent or following activation of one partner as a sulfonyl halide or acid halide. The combined mitochondrial targeting-linker precursor moiety would have an appropriate functional group on the linker, such as an amine. Thus, a carbodiimide or other coupling agent may be used to form an amide from these moieties. For example, in the synthesis of MitoGamide, the dicarbonyl sequestering group armed with a carboxylic acid can be reacted with a TPP-linker-amine using a coupling agent to form an amide. In theory, a dicarbonyl sequestering group-amine could be reacted with TPP-linker-carboxylic acid using a coupling agent provided that the reacting amine is more nucleophilic than the dicarbonyl sequestering unit, or the latter is protected. Similarly, sulfonyl chlorides react rapidly with amines to form sulfonamides in the presence of relatively weak bases to remove HCl, again the sequestering group may need protection to avoid reacting with the sulfonyl chloride.
The compounds of Formula 1 may be prepared using the techniques described below. Some of the schemes and examples may omit details of common reactions, including oxidations, reductions, and so on, separation techniques (extraction, evaporation, precipitation, chromatography, filtration, trituration, crystallization, and the like), and analytical procedures, which are known to persons of ordinary skill in the art of organic chemistry. The details of such reactions and techniques can be found in a number of treatises, including Richard Larock, Comprehensive Organic Transformations, A Guide to Functional Group Preparations, 2nd Ed (2010), and the multi-volume series edited by Michael B. Smith and others, Compendium of Organic Synthetic Methods (1974 et seq.). Starting materials and reagents may be obtained from commercial sources or may be prepared using literature methods. Some of the reaction schemes may omit minor products resulting from chemical transformations (e.g., an alcohol from the hydrolysis of an ester, CO2 from the decarboxylation of a diacid, etc.). In addition, in some instances, reaction intermediates may be used in subsequent steps without isolation or purification (i.e., in situ).
In some of the reaction schemes and examples below, certain compounds can be prepared using protecting groups, which prevent undesirable chemical reaction at otherwise reactive sites. Protecting groups may also be used to enhance solubility or otherwise modify physical properties of a compound. For a discussion of protecting group strategies, a description of materials and methods for installing and removing protecting groups, and a compilation of useful protecting groups for common functional groups, including amines, carboxylic acids, alcohols, ketones, aldehydes, and so on, see T. W. Greene and P. G. Wuts, Protecting Groups in Organic Chemistry, 4th Edition, (2006) and P. Kocienski, Protective Groups, 3rd Edition (2005).
Generally, the chemical transformations described throughout the specification may be carried out using substantially stoichiometric amounts of reactants, though certain reactions may benefit from using an excess of one or more of the reactants. Additionally, many of the reactions disclosed throughout the specification may be carried out at about room temperature (RT) and ambient pressure, but depending on reaction kinetics, yields, and so on, some reactions may be run at elevated pressures or employ higher temperatures (e.g., reflux conditions) or lower temperatures (e.g., −78° C. to 0° C.). Any reference in the disclosure to a stoichiometric range, a temperature range, a pH range, etc., whether or not expressly using the word “range,” also includes the indicated endpoints.
Many of the chemical transformations may also employ one or more compatible solvents, which may influence the reaction rate and yield. Depending on the nature of the reactants, the one or more solvents may be polar protic solvents (including water), polar aprotic solvents, non-polar solvents, or some combination. Representative solvents include saturated aliphatic hydrocarbons (e.g., n-pentane, n-hexane, n-heptane, n-octane); aromatic hydrocarbons (e.g., benzene, toluene, xylenes); halogenated hydrocarbons (e.g., methylene chloride, chloroform, carbon tetrachloride); aliphatic alcohols (e.g., methanol, ethanol, propan-1-ol, propan-2-ol, butan-1-ol, 2-methyl-propan-1-ol, butan-2-ol, 2-methyl-propan-2-ol, pentan-1-ol, 3-methyl-butan-1-ol, hexan-1-ol, 2-methoxy-ethanol, 2-ethoxy-ethanol, 2-butoxy-ethanol, 2-(2-methoxy-ethoxy)-ethanol, 2-(2-ethoxy-ethoxy)-ethanol, 2-(2-butoxy-ethoxy)-ethanol); ethers (e.g., diethyl ether, di-isopropyl ether, dibutyl ether, 1,2-dimethoxy-ethane, 1,2-diethoxy-ethane, 1-methoxy-2-(2-methoxy-ethoxy)-ethane, 1-ethoxy-2-(2-ethoxy-ethoxy)-ethane, tetrahydrofuran, 1,4-dioxane); ketones (e.g., acetone, methyl ethyl ketone); esters (methyl acetate, ethyl acetate); nitrogen-containing solvents (e.g., formamide, N,N-dimethylformamide, acetonitrile, N-methyl-pyrrolidone, pyridine, quinoline, nitrobenzene); sulfur-containing solvents (e.g., carbon disulfide, dimethyl sulfoxide, tetrahydro-thiophene-1,1,-dioxide); and phosphorus-containing solvents (e.g., hexamethylphosphoric triamide).
Applications
A key aspect of the invention is the blocking or amelioration of damage caused by hyperglycaemia. The invention finds application in the treatment or prevention of downstream consequences of hyperglycaemia.
One application for a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, is for use in the prevention or treatment of hyperglycaemia.
Hyperglycaemia can occur as an age related syndrome. Hyperglycaemia can occur in sepsis, and/or in intensive care/emergency room admissions. Hyperglycaemia can occur as a complication of pregnancy.
Thus, prevention of hyperglycaemia can be important for subjects with a health state that increases the risk of hyperglycaemia compared to a normal healthy subject who does not have that health state. Suitably, there may be provided a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the prevention or treatment of hyperglycaemia in a subject with a health state selected from sepsis, pregnancy or a health state requiring critical care.
Most specifically, the invention is applied in diabetes such as hyperglycemic diabetes. The invention finds application in the treatment or prevention of the downstream consequences of hyperglycaemia and diabetes (such as hyperglycemic diabetes), for example, to prevent or reduce damage to any suitable organs and tissues such as eyes, heart, kidneys, nervous system and/or pancreas.
The invention is applied to the treatment or prevention of mitochondrial damage associated with dysfunction or dysregulation of glycaemic control, for example mitochondrial damage associated with hyperglycaemia.
Most suitably the invention is applied to the treatment or prevention of molecular consequences of hyperglycaemia.
In one aspect, the invention relates to a compound of Formula 1 or a pharmaceutical salt thereof, for use in slowing the deleterious effects of aging in a subject.
In another aspect, the invention relates to a compound of Formula 1 or a pharmaceutical salt thereof, for use in preventing or reducing glycation-induced toxicity.
In another aspect, the invention relates the invention relates to a compound of Formula 1 or a pharmaceutical salt thereof, for use in preventing or reducing respiratory depression, in particular dicarbonyl-induced respiratory depression.
In another aspect, the invention relates the invention relates to a compound of Formula 1 or a pharmaceutical salt thereof, for use to protect against cell death, in particular to protect against glycation-induced cell death.
A further aspect of the invention provides a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the preservation of organ and tissue for surgical transplants. In such an application the compound of formula 1 or a pharmaceutically acceptable salt thereof may suitably be administered as a component of a tissue preservation fluid. In this aspect, any suitable organ and tissue for surgical transplants may be preserved; illustrative organ transplants include eyes (e.g. cornea), heart, kidney, nerve cells and pancreas.
A further aspect of the invention provides a compound of formula 1, as defined above, or a pharmaceutically acceptable salt thereof, for use in the storage of blood. In such an application the compound of formula 1 or a pharmaceutically acceptable salt thereof may suitably be administered as a component of a preservation fluid.
Suitably the invention is applied to mammals, most suitably humans.
The invention may be applied to retinal degeneration. Retinal degeneration is a correlate of diabetic complications. The invention may be usefully applied to any other complication of diabetes, specifically hyperglycemic diabetes.
Administration
The compound of the invention can be administered in solid or liquid form such as tablets, powders, capsules, pellets, solutions, suspensions elixirs, emulsions, gels, creams, or suppositories, including rectal and urethral suppositories.
Suitably compounds of the invention may be used to treat humans.
Suitably the dose for humans is orally administered.
Suitably the dose for humans is one per day.
Suitably the dose for humans is provided in tablet form.
The compounds or compositions of the invention may be administered by parenteral administration.
The compound of the invention may be administered intravenously.
The compound of the invention can be administered in a dose of approximately 20-40 mgs per day. Suitably the dose for humans is approximately 80 mgs per day.
Dosing information is provided for an average adult human unless otherwise stated. It is well within the skill of a physician to vary the dose according to characteristics of the patient being treated, for example age, weight, gender etc.
The compound of the invention may be administered intraocularly.
The compound of the invention may be administered intravenously.
The compound of the invention may be administered intraperitoneally.
The compound of the invention may be administered as eye drops.
Administration may be to any suitable tissue or organ, for example, the compounds or compositions of the invention may be administered to the eye, heart, kidney, nervous system and/or pancreas.
The compound of the invention may be administered in combination with one or more pharmaceutically acceptable excipients, carriers or diluents.
Suitable excipients, carriers and diluents can be found in standard pharmaceutical texts. See, for example, Handbook for Pharmaceutical Additives, 3rd Edition (eds. M. Ash and I. Ash), 2007 (Synapse Information Resources, Inc., Endicott, N.Y., USA) and Remington: The Science and Practice of Pharmacy, 21st Edition (ed. D. B. Troy) 2006 (Lippincott, Williams and Wilkins, Philadelphia, USA) which are incorporated herein by reference.
Excipients for use in the compositions of the invention include, but are not limited to microcrystalline cellulose, sodium citrate, calcium carbonate, dicalcium phosphate and glycine may be employed along with various disintegrants such as starch (and preferably corn, potato or tapioca starch), alginic acid and certain complex silicates, together with granulation binders like polyvinylpyrrolidone, sucrose, gelatin and acacia. Additionally, lubricating agents such as magnesium stearate, sodium lauryl sulfate and talc are often very useful for tabletting purposes. Solid compositions of a similar type may also be employed as fillers in gelatin capsules; preferred materials in this connection also include lactose or milk sugar as well as high molecular weight polyethylene glycols. When aqueous suspensions and/or elixirs are desired for oral administration, the active ingredient may be combined with various sweetening or flavouring agents, colouring matter or dyes, and, if so desired, emulsifying and/or suspending agents as well, together with such diluents as water, ethanol, propylene glycol, glycerin and various like combinations thereof.
Pharmaceutical carriers include solid diluents or fillers, sterile aqueous media and various non-toxic organic solvents, and the like.
Pharmaceutically acceptable carriers include gums, starches, sugars, cellulosic materials, and mixtures thereof. The compound can be administered to a subject by, for example, subcutaneous implantation of a pellet. The preparation can also be administered by intravenous, intra-arterial, or intramuscular injection of a liquid preparation oral administration of a liquid or solid preparation, or by topical application. Administration can also be accomplished by use of a rectal suppository or a urethral suppository.
Further, as used herein “pharmaceutically acceptable carriers” are well known to those skilled in the art and include, but are not limited to, 0.01-0.1M and preferably 0.05M phosphate buffer or 0.9% saline. Additionally, such pharmaceutically acceptable carriers may be aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media.
Pharmaceutically acceptable parenteral vehicles include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Preservatives and other additives may also be present, such as, for example, antimicrobials, antioxidants, collating agents, inert gases and the like.
Pharmaceutically acceptable carriers for controlled or sustained release compositions administrable according to the invention include formulation in lipophilic depots (e.g. fatty acids, waxes, oils). Also comprehended by the invention are particulate compositions coated with polymers (e.g. poloxamers or poloxamines) and the compound coupled to antibodies directed against tissue-specific receptors, ligands or antigens or coupled to ligands of tissue-specific receptors.
Pharmaceutically acceptable carriers include compounds modified by the covalent attachment of water-soluble polymers such as polyethylene glycol, copolymers of polyethylene glycol and polypropylene glycol, carboxymethyl cellulose, dextran, polyvinyl alcohol, polyvinylpyrrolidone or polyproline are known to exhibit substantially longer half-lives in blood following intravenous injection than do the corresponding unmodified compounds (Abuchowski and Davis, Soluble Polymer-Enzyme Adducts, Enzymes as Drugs, Hocenberg and Roberts, eds., Wiley-Interscience, New York, N.Y., (1981), pp 367-383; and [65]). Such modifications may also increase the compound's solubility in aqueous solution, eliminate aggregation, enhance the physical and chemical stability of the compound, and greatly reduce the immunogenicity and reactivity of the compound. As a result, the desired in vivo biological activity may be achieved by the administration of such polymer-compound abducts less frequently or in lower doses than with the unmodified compound.
Mass Spectrometry
The invention also finds application as a mass spectrometry probe for detection of reactive dicarbonyls.
These mass spectrometry methods enable the assessment of the importance of mitochondrial damage caused by methylglyoxal and glyoxal. Hence, the compounds of Formula 1, for example, the mitochondria-selective molecule MitoG, may be used to assess relative changes in the levels of these damaging species within mitochondria in cells and in vivo.
The mode of action of MitoG, is shown in
Further particular and preferred aspects are set out in the accompanying independent and dependent claims. Features of the dependent claims may be combined with features of the independent claims as appropriate, and in combinations other than those explicitly set out in the claims.
Where an apparatus feature is described as being operable to provide a function, it will be appreciated that this includes an apparatus feature which provides that function or which is adapted or configured to provide that function.
Embodiments of the present invention will now be described further, with reference to the accompanying drawings, in which:
Although illustrative embodiments of the invention have been disclosed in detail herein, with reference to the accompanying drawings, it is understood that the invention is not limited to the precise embodiment and that various changes and modifications can be effected therein by one skilled in the art without departing from the scope of the invention as defined by the appended claims and their equivalents.
Materials and Methods
Chemical Syntheses
A schematic of the syntheses of MitoG, quinoxaline ether (QE) and the methylquinoxaline ethers 1 and 2 (MQE1/MQE2) is shown in
To a solution of 6-(4-aminophenoxy)hexan-1-ol 1 (2.12 g, 10.0 mmol) in glacial acetic acid (3.20 mL) and water (2.40 mL) with ice (3.70 g) at 0-5° C. was added acetic anhydride (1.20 mL) with rapid stirring. The resulting crystalline mixture was dissolved by heating in a water bath. The reaction mixture was then cooled to about 45° C. before the addition of concentrated nitric acid (1.10 mL) with stirring. The reaction mixture was heated at 65° C. for 10 min, cooled to room temperature, placed in an ice bath for 18 h, diluted with water and extracted into dichloromethane. The organic phase was dried (MgSO4) and evaporated in vacuo to give a mixture of 2, together with an intermediate acetate, as a yellow oil (2.98 g). Purification by column chromatography on silica gel eluting with diethyl ether gave 2 as a yellow crystalline solid (1.279 g, 43%).
1H NMR (500 MHz, CDCl3): δ 10.03 (1H, bs, NH), 8.61 (1H, d, J=8 Hz), 7.64 (1H, d, J=2 Hz), 7.21 (1H, dd, J=2, 8 Hz), 3.99 (2H, t, J=6 Hz, CH2O), 3.67 (2H, t, J=6 Hz, CH2OH), 2.26 (3H, s, Ac), 1.79-1.82 (2H, m), 1.58-1.64 (2H, m), 1.41-1.54 (4H, m), 1.30 (1H, bs, OH) ppm; 13C NMR (125 MHz, CDCl3): δ 168.8, 154.5, 137.1, 128.4, 123.9, 123.8, 109.2, 68.7 (CH2O), 62.9 (CH2OH), 32.7, 29.0, 25.9, 25.6, 25.5 ppm; MS m/z found: 319.1261, calcd. for C14H20N2O5.Na+, 319.1264.
Claisen's alkali (0.45 mL) was added to the total crude product from 2 (0.527 g, 1.78 mmol) and heated to 70° C. with stirring for 15 min. Hot water (0.45 mL) was then added to the reaction mixture with stirring and this was heated for another 15 min. The reaction mixture was cooled to 0-5° C. in an ice bath, diluted with water and extracted into dichloromethane. The organic phase was dried (MgSO4) and evaporated in vacuo to give an orange/red solid (0.390 g). Purification by column chromatography on silica gel eluting with diethyl ether gave 3 as an orange/red solid (0.236 g, 52%).
1H NMR (500 MHz, CDCl3): δ 7.53 (1H, d, J=2 Hz), 7.06 (1H, dd, J=2, 8 Hz), 6.75 (1H, d, J=8 Hz), 5.88 (2H, bs, NH2), 3.92 (2H, t, J=6 Hz, CH2O), 3.66 (2H, bt, J=6 Hz, CH2OH), 1.76-1.81 (2H, m, CH2CH2O), 1.58-1.65 (2H, m, CH2CH2OH), 1.40-1.52 (4H, m), 1.30 (1H, bs, OH); 13C NMR (125 MHz, CDCl3): δ 150.3, 139.9, 131.6, 127.1, 120.1, 107.2, 68.7 (CH2O), 62.9 (CH2OH), 32.7, 29.1, 25.9, 25.6; MS m/z found: 277.1161, calcd. for C12H18N2O4.Na+: 277.1159. Microanalysis found: C, 56.83; H, 6.94; N, 10.99; calcd. for C12H18N2O4: C, 56.68; H, 7.13; N, 11.02.
Palladium on carbon (0.20 g) was added to a solution of the nitroaniline (3) (2.22 g, 8.7 mmol) in dry ethanol (200 mL) and the mixture stirred for 18 h under an atmosphere of hydrogen. The reaction mixture was filtered through Celite® and evaporated in vacuo to give 4 as a red solid (1.83 g, 93%). The crude product was used without further purification.
1H NMR (500 MHz, CDCl3): δ 6.62 (1H, d, J=8 Hz), 6.32 (1H, d, J=2 Hz), 6.25 (1H, dd, J=2, 8 Hz), 3.87 (2H, t, J=6 Hz, CH2O), 3.65 (2H, t, J=6 Hz, CH2OH), 1.72-1.77 (2H, m, CH2CH2O), 1.55-1.65 (2H, m, CH2CH2OH), 1.38-1.51 (4H, m); 13C NMR (125 MHz, CDCl3): δ 154.0, 137.1, 127.3, 118.4, 105.1, 103.8, 68.3 (CH2O), 63.0 (CH2OH), 32.8, 29.4, 26.0, 25.6; MS m/z found: 225.1592, calcd. for C12H21N2O2+, 225.1596; Microanalysis found: C, 64.15; H, 8.94, 12.29; calcd. for C12H20N2O2: C, 64.26; H, 8.99; N, 12.49.
Di-tert-butyldicarbonate (0.48 g, 2.2 mmol) was added to a solution of 4 (0.16 g, 0.73 mmol) in tetrahydrofuran (5 mL) and stirred under an atmosphere of argon at room temperature for 18 h. Sodium bicarbonate (0.31 g, 3.6 mmol) was added to the reaction mixture which was stirred for another 30 min. The reaction mixture was filtered and evaporated in vacuo to give a yellow oil (0.621 g). Purification by column chromatography on silica gel eluting with 1% triethylamine in diethyl ether gave 5 as a yellow oil (0.228 g, 74%).
1H NMR (500 MHz, CDCl3): δ 7.29 (1H, bs), 7.14 (1H, m), 6.91 (1H, bs, NH), 6.59 (1H, dd, J=2, 8 Hz), 6.35 (1H, bs, NH), 3.93 (2H, t, J=6 Hz, CH2O), 3.63 (2H, t, J=6 Hz, CH2OH), 1.72-1.78 (2H, m), 1.54-1.63 (2H, m), 1.50 (9H, s), 1.49 (9H, s), 1.36-1.47 (4H, m) ppm; 13C NMR (125 MHz, CDCl3): δ 157.6 (C4), 154.6, 153.3, 133.6 (C2), 126.7 (C6), 121.0 (C1), 110.8 (C5), 108.4 (C3), 80.8, 80.7, 68.1 (CH2O), 62.9 (CH2OH), 32.7 (CH2CH2O), 29.2 (CH2CH2OH), 28.4, 28.3, 25.9, 25.5; MS m/z found: 447.2451, calcd. for C22H36N2O6.Na+: 447.2466; Microanalysis found: C, 62.50; H, 8.86; N, 6.35; calcd. for C22H36N2O6: C, 62.24; H, 8.86; N, 6.35.
A solution of 5 (0.23 g, 0.53 mmol) and triethylamine (150 μL, 0.110 g, 1.06 mmol) was stirred in anhydrous dichloromethane (10 mL) at room temperature. Methanesulfonyl chloride (50 μL, 0.074 g, 0.64 mmol) was added and the reaction was stirred for 1 h. The reaction mixture was diluted with dichloromethane, washed with water and aqueous sodium bicarbonate, dried (MgSO4) and evaporated in vacuo to give 6 as a yellow oil (0.213 g, 80%). The crude product was used without further purification.
1H NMR (500 MHz, CDCl3): δ 7.31 (1H, bs), 7.15 (1H, m), 6.89 (1H, bs, NH), 6.59 (1H, dd, J=2, 8 Hz), 6.29 (1H, bs, NH), 4.23 (2H, t, J=8 Hz, CH2OMs), 3.94 (2H, t, J=6 Hz, CH2O), 2.99 (3H, s, Ms), 1.74-1.80 (4H, m), 1.46-1.51 (22H, m) ppm; 13C NMR (125 MHz, CDCl3): δ 157.6, 154.6, 153.3, 133.6, 126.8, 121.0, 110.8, 108.3, 80.8, 80.7, 70.0 (CH2OMs), 67.9 (CH2O), 37.4, 29.1, 29.0, 28.4, 28.3, 25.6, 25.3 ppm; MS m/z found: 525.2240, calcd. for C23H38N2O8S.Na+: 525.2241.
A solution of mesylate 6 (0.59 g, 1.17 mmol) and triphenylphosphine (0.34 g, 1.29 mmol) with sodium iodide (0.260 g, 1.76 mmol) in dry acetonitrile (100 mL) was heated to 80° C. with stirring for 48 h. The reaction mixture was cooled to room temperature and the white solid was removed by filtration and washed with acetonitrile. The solvent was then reduced to about 30 mL and ether (400 mL) added slowly with stirring to precipitate the product. The ether was decanted from the oily solid and the process was repeated. The oily solids were dried at <0.5 mm Hg for 1 h to give a yellow solid (0.680 g). Purification by column chromatography on silica gel eluting with 5 to 15% methanol in chloroform gave 7 as a white solid (0.585 g, 63%).
1H NMR (500 MHz, CD2Cl2): δ 7.84-7.80 (3H, m, Ar), 7.73-7.67 (12H, m, Ar), 7.54 (1H, bs, NH), 7.25 (1H, bs), 7.19 (1H, d, J=8 Hz), 6.94 (1H, bs, NH), 6.55 (1H, dd, J=2, 8 Hz), 3.91 (2H, t, J=6 Hz, CH2O), 3.31 (2H, m, CH2P), 1.59-1.72 (6H, m), 1.26-1.47 (20H, m); 13C NMR (125 MHz, CDCl3): δ 157.3, 154.8, 153.8, 135.6 (d, JC4P=3 Hz, p-Ph), 133.9 (d, JC2P=10 Hz, o-Ph), 130.9 (d, JC3P=13 Hz, m-Ph), 126.9, 122.4, 118.5 (d, JCP=86 Hz, i-Ph), 110.7, 109.0, 80.5, 80.4, 68.1 (CH2O), 30.2 (d, JC3P=16 Hz, CH2CH2CH2P), 28.9, 28.4, 28.3, 25.6, 22.7 (d, JC2P=4 Hz, CH2CH2P), 22.6 (d, JCP=51 Hz, CH2P); 31P NMR (162 MHz, CD2Cl2): δ 24.4 ppm; MS m/z found: 669.3506, calcd. for C40H50N2H5P+: 669.3452;
A sample of the tetraphenylborate salt of 7 for analysis was prepared by anion exchange with sodium tetraphenylborate in dichloromethane. The product was recrystallised from ethanol at −78° C., filtered and dried in vacuo at 40° C. for 4 days to give (6-(3,4-bis(tert-butoxycarbonylamino)phenoxy)hexyl)triphenylphosphonium tetraphenylborate as white crystals.
Microanalysis found: C, 77.43; H, 7.35; N, 2.80; calcd. for C64H70N2O5BP: C, 77.72; H, 7.13; N, 2.83; MS m/z found: 669.3442, calcd. for C40H50N2H5P+: 669.3452. found: 319.1680, calcd. for C24H20B−: 319.1664. 1H and 31P NMR spectra were consistent with 7.
Similarly mesylate (6) (290 mg) with sodium iodide (110 mg) and d15 labelled triphenylphosphine (100 mg) gave d15-7 as an off white gum (0.274 g, 90%). HPLC 13.24 min 99+% pure. 1H NMR (500 MHz, CDCl3): δ 7.25 (1H, bs), 7.16 (1H, bd, J=8 Hz), 6.96 (1H, bs, NH), 6.57 (1H, dd, J=2, 8 Hz), 6.38 (1H, bs, NH), 3.89 (2H, t, J=6 Hz, CH2O), 3.74 (2H, m, CH2P), 1.59-1.72 (6H, m), 1.55 (6H, s) and 1.38-1.48 (14H, m). MS m/z found: 684.396, calcd. for C40H35D15N2O5P+: 684.4393.
Hydrochloric acid (9.8 M, 1.0 mL) was added to a solution of 7 (0.050 g, 0.065 mmol) in 1,4-dioxane (1 mL) and stood at room temperature under argon for 1 h. The reaction mixture was concentrated under reduced pressure (<0.5 mmHg) to give MitoG as a pale yellow solid (0.035 g, 98%).
HPLC 8.9 min 95% pure, 1H NMR (500 MHz, (CD3)2SO): δ 7.86-7.90 (3H, m, Ar), 7.73-7.80 (12H, m, Ar), 6.39 (1H, d, J=8 Hz), 6.12 (1H, d, J=2 Hz), 5.92 (1H, dd, J=2, 8 Hz), 4.47 (4H, bs, NH2), 3.70 (2H, t, J=6 Hz, CH2O), 3.57 (2H, m, CH2P), 1.45-1.58 (6H, m), 1.34-1.40 (2H, m); 13C NMR (125 MHz, (CD3)2SO): δ 151.6, 136.7, 134.8 (d, JC4P=3 Hz, p-Ph), 133.5 (d, JC2P=10 Hz, o-Ph), 130.2 (d, JC3P=12 Hz, m-Ph), 127.8, 118.5 (d, JCP=86 Hz, i-Ph), 115.5, 102.4, 101.9, 67.2 (CH2O), 29.6 (d, JC3P=17 Hz, CH2CH2CH2P), 28.6, 24.8, 21.7 (d, JC2P=4 Hz, CH2CH2P), 20.2 (d, JCP=50 Hz, CH2P) ppm; 31P NMR (162 MHz, CD2Cl2): δ 23.8 ppm; MS m/z found: 469.2404, calcd. for C30H34N2OP+: 469.2403.
Similarly (6-(3,4-bis(tert-butoxycarbonylamino)phenoxy)hexyl)triphenylphosphonium iodide (d15-7) (0.021 g, 0.026 mmol) gave d15-MitoG as a pale yellow solid. The crude product was used without further purification.
HPLC: 9.0 min 89% pure. MS m/z found: 484.3332, calcd. for C30H19D15N2OP+: 484.3345.
Triethylamine (50 μL) was added to a stirred solution of 8 (0.007 g, 14 μmol) in ethanol (96%, 1 mL) under argon at room temperature. After 5 min a solution of glyoxal (40%, 50 μL) in ethanol (96%, 0.5 mL) was added and the mixture stirred under argon for 2 h. The reaction mixture was evaporated in vacuo to give the product as a pale yellow gum. Purification by column chromatography on an SPE C18 silica column (10 g), eluting stepwise with 10 to 100% acetonitrile in water (0.1% TFA) gave QE in the 50% fraction as a brown gum (5 mg, 68%).
HPLC 11.2 min 99+% pure; 1H NMR (500 MHz, CD2Cl2): δ 8.72 (1H, d, J=2 Hz), 8.68 (1H, d, J=2 Hz), 7.96 (1H, d, J=8 Hz), 7.77-7.84 (3H, m, Ar), 7.69-7.76 (12H, m, Ar), 7.40 (1H, dd, J=2, 8 Hz), 7.38 (1H, d, J=2 Hz), 4.12 (2H, t, J=6 Hz, CH2O), 3.48 (2H, m, CH2P), 1.85 (2H, m), 1.72 (4H, m), 1.56 (2H, m) ppm; 13C NMR (125 MHz, CD2Cl2): δ 160.6, 144.9, 144.6, 142.7, 139.6, 135.6 (d, JC4P=3 Hz, p-Ph), 134.0 (d, JC2P=10 Hz, o-Ph), 130.9 (d, JC3P=13 Hz, m-Ph), 130.7, 123.9, 118.4 (d, JCP=86 Hz, i-Ph), 107.5, 68.8 (CH2O), 30.6 (d, JC3P=16 Hz, CH2CH2CH2P), 29.0, 25.9, 23.5 (d, JCP=51 Hz, CH2P), 22.9 (d, JC2P=4 Hz, CH2CH2P) ppm; 31P NMR (162 MHz, CD2Cl2): δ 23.8 ppm; MS m/z found: 491.2238, calcd. for C32H32N2OP+: 491.2247.
Similarly diamine (d15-MitoG) (0.036 g, 71 μmol) with glyoxal (40%, 200 μL) gave d15-QE in the 50% fraction as a brown gum (14 mg, 37%).
HPLC 11.0 min 99+% pure; 1H NMR (500 MHz, CDCl3): δ 8.79 (1H, d, J=2 Hz), 8.74 (1H, d, J=2 Hz), 8.05 (1H, d, J=8 Hz), 7.43 (1H, dd, J=2, 8 Hz), 7.38 (1H, d, J=2 Hz), 4.12 (2H, t, J=6 Hz, CH2O), 3.38 (2H, m, CH2P), 1.82 (2H, m), 1.66 (4H, m), 1.53 (2H, m) ppm; MS m/z found: 506.3201, calcd. for C32H17D15N2OP+: 506.3188.
Similarly reaction of MitoG (0.007 g, 14 μmol) with methylglyoxal (40%, 50 μL) gave the methyl quinoxalines (MQE1/MQE2) in the 50% fraction as a brown gum (5 mg, 68%).
HPLC: 11.1 min. 1H NMR (500 MHz, CD2Cl2): δ 8.78 (1H, s), 8.04 (1H, d, J=8 Hz), 7.77-7.84 (3H, m, Ar), 7.69-7.76 (12H, m, Ar), 7.55 (1H, d, J=2 Hz), 7.44 (1H, dd, J=2, 8 Hz), 4.16 (2H, t, J=6 Hz, CH2O), 3.14 (2H, m, CH2P), 2.83 (3H, s, C-Me), 1.88 (2H, m, CH2CH2O), 1.75 (2H, m), 1.67 (2H, m) and 1.58 (2H, m) ppm; 13C NMR (125 MHz, CD2Cl2): δ 161.91, 160.25 (q, JCF=30 Hz, CF3CO), 152.74, 143.20, 141.10, 137-43, 135.80 (d, JC4P=3 Hz, p-Ph), 133.58 (d, JC2P=10 Hz, o-Ph), 130.92 (d, JC3P=13 Hz, m-Ph), 130.08, 123.83, 117.88 (d, JCP=86 Hz, i-Ph), 116.21 (d, JCF=230 Hz, CF3) 105.01, 68.88 (CH2O), 30.50 (d, JC3P=16.0 Hz, CH2CH2CH2P), 28.71, 25.58, 23.20 (d, JCP=51 Hz, CH2P), 22.69 (d, JC2P=4 Hz, CH2CH2P), 21.06 (CMe) ppm; 31P NMR (162 MHz, CD2Cl2): δ 23.8 ppm. MS m/z found: 505.2419, calcd. for C33H34N2OP+: 505.2403.
Similarly d15-MitoG (0.036 g, 71 μmol) with methylglyoxal (40%, 200 μL) gave d15-MQE1/MQE2 in the 50% fraction as a brown gum (16 mg, 43%).
HPLC: 11.1 min 99%+. 1H NMR (500 MHz, CDCl3): δ 8.61 (2H, bs), 7.93 (1H, d, J=8 Hz), 7.32 (1H, dd, J=2, 8 Hz), 7.31 (1H, s), 4.07 (2H, t, J=6 Hz, CH2O), 3.50 (2H, m, CH2O), 2.75 (3H, s, CMe), 1.82 (2H, m, CH2CH2O), 1.66 (4H, m), 1.51 (2H, m) ppm; MS m/z found: 520.3366, calcd. for C33H19D15N2OP+: 520.3345.
The control compound (HP) was made as described above for MitoG. The compounds are related to the numbering scheme used in
Briefly, N-(4-(6-hexyloxy)-2-nitrophenyl)acetamide (2b) [62] was made from the amine 4-(hexyloxy)aniline 1b [32](1.57 g, 8.12 mmol) and gave 2b as a yellow oil (2.05 g). Purification by column chromatography on silica gel eluting with dichloromethane/diethyl ether gave 2b as a yellow solid (1.56 g, 75%). 1H NMR (400 MHz, CDCl3): δ 10.04 (1H, bs), 8.62 (1H, d, J=8 Hz), 7.65 (1H, d, J=2 Hz), 7.22 (1H, dd, J=2, 8 Hz), 3.99 (2H, t, J=6 Hz, CH2O), 2.26 (3H, s, Ac), 1.80 (2H, m, CH2CH2O), 1.46 (2H, m), 1.35 (4H, m) and 0.91 (3H, t, J=6 Hz, CH3) ppm. Reaction of 2b (0.42 g, 1.5 mmol) gave then 4-(hexyloxy)-2-nitroaniline 3b then 4-hexyloxy-1,2-phenylenediamine 4b [63] as a red solid (0.224 g, 72%). This was used directly in the following reaction. For this the diamine 4b (0.34 g, 1.5 mmol) gave 3,4-bis tert-butyl-4-(hexyloxy)-1,2-phenylenedicarbamate 5b as a yellow oil (0.430 g, 71%). HPLC: 17.7 min>99% pure. 1H NMR (500 MHz, CDCl3): δ 7.31 (1H, bs), 7.16 (1H, m), 6.84 (1H, bs, NH), 6.62 (1H, dd, J=2, 8 Hz), 6.25 (1H, bs, NH), 3.93 (2H, t, J=6 Hz, CH2O), 1.75 (2H, m, CH2CH2O), 1.52 (9H, s), 1.50 (9H, s), 1.46 (2H, m), 1.33 (4H, m) and 0.90 (3H, t, J=6 Hz, CH3); MS m/z found: 407.2618, calcd. for C22H35N2O5−: 407.2546.
To make 4-hexyloxybenzene-1,2-diamine (HP) 5b (0.022 g, 0.054 mmol) was reacted with hydrochloric acid to give HP as a pale yellow solid. HPLC: 9.67 min>99% pure.
1H NMR (500 MHz, (CD3)2SO): δ 7.19 (1H, d, J=8 Hz), 6.54 (1H, d, J=2 Hz), 6.37 (1H, dd, J=2, 8 Hz), 5.60 (4H, bs, NH2), 3.95 (2H, t, J=6 Hz, CH2O), 1.79 (2H, m, CH2CH2O), 1.50 (2H, m), 1.41 (4H, m), 1.51 (3H, t, J=6 Hz, CH3) ppm. 13C NMR (125 MHz, (CD3)2SO): δ 159.24, 142.83, 125.17, 111.50, 104.30, 103.01, 67.87 (CH2O), 31.44, 29.04, 25.61, 22.52 and 14.36 (CH3) ppm; MS m/z found: 209.1701, calcd. for C12H21N2O+: 209.1654.
A solution of (3-aminopropyl)triphenylphosphonium iodide (1.34 g, 3.0 mmol), 2,4-diaminobenzoic acid (445 mg, 2.9 mmol), and diisopropylcarbodiimide (400 μl, 2.6 mmol) in dry MeCN (10 mL) was stirred at 35° C. under Ar for 48 h. After this, the precipitate was washed with MeCN (3×10 mL). The solid was collected and the residual solvent was removed under high vacuum. The resulting brown solid (1.25 g) was recrystallized twice from ethanol to give the iodide salt as a brown powder (790 mg, 53%). A sample of the iodide was dissolved in MeOH—H2O (50:50) and passed trough an ion exchange column (Amberlite IRA-400, mesylate counterion) twice. The solvent was removed under vacuum yielding the phosphonium mesylate as an amorphous brown solid (238 mg, 44%). δH (CD3OD, 400 MHz): 7.92-7.87 (m, 3×p-H PPh3), 7.82-7.71 (12H, m, 6×o-H PPh3, 6×m-H PPh3), 7.21 (1H, d, J=1.9 Hz, H-2′), 7.14 (1H, dd, J=8.1, 2.0 Hz, H-6′), 6.68 (1H, d, J=8.1 Hz, H-5′), 3.53 (2H, t, J=6.4 Hz, NCH2), 3.50-3.44 (2H, m, PCH2), 2.70 (3H, s, CH3SO3) 2.02-1.92 (2H, m, CH2CH2CH2). δC (CD3OD, 125 MHz): 170.99 (C), 141.14 (C), 136.34 (d, J=4 Hz, CH), 134.80 (d, J=10 Hz, CH), 131.59 (d, J=12 Hz, CH), 124.42 (C), 120.52 (CH), 119.78 (d, J=86 Hz, C), 116.44 (CH), 115.55 (CH), 40.81 (d, J=18 Hz, CH2), 39.55 (CH3), 24.03 (d, J=4 Hz, CH2), 20.68 (d, J=52 Hz, CH2) (one C presumably coincident with another signal).
The glyoxalase I inhibitor, bromobenzyl glutathione cyclopentyl diester, was synthesised by alkylation of glutathione [35], followed by standard Boc-protection, coupling with cyclopentanol using 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate [36], trifluoroacetic acid (TFA) removal of the Boc group and treatment with sodium bicarbonate to give the free base. Other reagents were obtained from Sigma-Aldrich, unless otherwise stated. 1H, 13C and 31P spectra were acquired on Varian INOVA-400 or Varian INOVA-500 spectrometers. Chemical shifts are reported downfield from TMS, relative to the solvent residual signal. For the chemical synthesis components of this work, high resolution mass spectra were recorded on a Bruker microTOF electrospray mass spectrometer and HPLC analysis was carried out on an Agilent HP1100: Column Phenomenex Prodigy 250×3 mm, gradient elution 10% acetonitrile/water (0.1% TFA) to 100% acetonitrile over 12.5 min at 0.5 mL min-1 with detection at 210 and 254 nm.
Assessment of Compound Properties
TPP-conjugated compounds were made up as 10 mM stock solutions in DMSO, flushed with argon and stored as aliquots at −20° C. UV/Visible spectra and kinetic analyses were done using a Shimadzu UV-2501PC spectrophotometer in a 1 ml-cuvette containing KCl buffer [120 mM KCl, 10 mM HEPES and 1 mM EGTA, pH 7.2 (KOH)]. The molar extinction coefficients were calculated from the absorbance at maximal absorption (λmax) for the compounds for solutions of known concentration. Reaction rates between MitoG and 1,2-dicarbonyls were determined from the initial linear slope over the first 30 s at the λmax for the formation of the relevant quinoxaline. Fluorescence spectra were obtained in 2.5 ml KCl buffer using a Shimadzu RF-301PC fluorimeter with slit widths of 3 nm. Kinetic assays used excitation and emission wavelengths of 344 and 433 nm, respectively, emission spectra used an excitation wavelength of 344 nm and excitation spectra used an emission wavelength of 433 nm.
RP-HPLC was performed using a Gilson 321 pump with a C18 column (Jupiter 300 Å, Phenomenex) with a Widepore C18 guard column (Phenomenex). Samples (1 ml) were injected through a 0.22 μm PVDF filter (Millex, Millipore). HPLC buffer A [0.1% TFA in water] and HPLC buffer B (90% acetonitrile and 0.1% TFA) were used and a gradient was run at 1 ml/min at room temperature as follows: 0-2 min—5% B; 2-17 min—5-100% B; 17-19 min—100% B; 19-22 min—100-5% B. Peaks were detected by absorbance at 220 nm (UV/Vis 151, Gilson) and by fluorescence (excitation and emission wavelengths of 344 and 433 nm; RF-10AXL, Shimadzu). Partition coefficients between PBS and octan-1-ol were determined as previously described [37].
Mitochondrial Preparation and Incubations
Rat liver mitochondria (RLM) were prepared at 4° C. in STE buffer [250 mM sucrose, 5 mM Tris and 1 mM EGTA, pH 7.4 (HCl)] by homogenisation and differential centrifugation. Protein concentration was determined using the biuret assay relative to bovine serum albumin (BSA) and was typically 40-60 mg/ml. A Clark-type oxygen electrode (Rank Brothers, Bottisham, Cambridge, UK) connected to a Powerlab 2/20 data acquisition system (AD Instruments, Australia) was used to measure respiration rates and was calibrated with air-saturated water (210 nmol O2/ml at 37° C.). RLM (2 mg protein/ml) were suspended in 120 mM KCl, 10 mM HEPES, 1 mM EGTA, 1 mM MgCl2 and 5 mM KH2PO4, pH 7.2 (KOH) in a thermostatted 1 ml-electrode chamber with stirring. MitoG was then added and after 5 min glutamate and malate (5 mM of each) were added, followed 3 min later by 400 μM ADP. Oxygen consumption rates were determined from the slopes using Chart v5.5.6. for Mac (AD Instruments, Australia). An electrode selective for the TPP moiety of MitoG was constructed and used as previously described [38].
Cell Culture
All cells were incubated at 37° C. in a humidified atmosphere of 95% air and 5% CO2 and culture media used were supplemented with 10% (v/v) fetal calf serum (FCS), 100 U/ml penicillin and 100 μg/ml streptomycin. C2C12 cells (mouse myoblast cell line; European Collection of Animal Cell Cultures) were cultured in low glucose (1000 mg/l D-glucose) Dulbecco's modified Eagle's medium (DMEM; Invitrogen). Cells were maintained at sub-confluence (<80%) to prevent differentiation. BAECs (Bovine Aortic Endothelial Cells; Cell Applications Inc, San Diego, Calif.) were maintained in α-minimum essential medium (αMEM; Invitrogen) containing 5 mM D-glucose. All culture flasks and assay plates used for experiments were pre-coated with fibronectin ((25 μg/ml: Sigma) in Hank's balanced salt solution (HBSS; Cell Applications) at 1-4 μg fibronectin/cm2. After 1 h at RT excess coating solution was removed, and flasks and plates were then seeded with cells for experiments. BAECs were used for experiments at passages 4-6.
To assess cell viability, C2C12 cells or BAECs were seeded at a density of 10,000 cells/well and 40,000 cells/well, respectively, in 96-well plates. After an overnight incubation, the medium was replaced with fresh media containing test compounds and incubated for 24 h. To determine cell survival, cells were washed twice with media, then fresh medium (100 μl per well) was added and mixed with 20 μl [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]/phenazine methosulphate (MTS/PMS) (Promega, USA). After 2 h absorbance was read at 490 nm in a plate reader (SpectraMax Plus 384, Molecular Devices). All treatments were conducted in triplicate wells.
The Seahorse XF24 extracellular flux analyser was used to assess cellular oxygen consumption rate (OCR) [39-41]. Cells (40,000 BAECs/well) were cultured and subjected to experimental treatment in Seahorse XF24 V7 assay plates that were coated with fibronectin as described above. OCR was determined as follows: cells were washed twice in assay medium [4.15 g/l DMEM base, 1.85 g/l NaCl, ix glutamax (Invitrogen), 1 mM sodium pyruvate, 5 mM D-glucose, 15 mg/l phenol red, 20 mM HEPES and 0.4% (v/v) FCS, pH 7.4] and incubated in 630 μl assay medium for 1 h at 37° C. in air. After 20 min equilibration basal OCR was determined and after 3 h incubation with MitoG sequential injections of oligomycin (1 μg/ml), FCCP (2 μM) and rotenone/antimycin A (4 μg/ml and 5 μM, respectively). OCRs were normalized to cell number as measured using the sulphorhodamine B (SRB) assay as follows: cells were fixed (200 μl 5% trichloroacetic acid at 4° C. for 1 h), washes with water (×3), stained for 20 min with 50 μl 0.4% (w/v) SRB in 1% (v/v) acetic acid, washed (3×1% acetic acid) and the incorporated SRB dye solubilised in 100 μl 10 mM Tris base for 5 min. Then 50 μl of each sample was transferred in duplicate to a 96-well plate, mixed with 50 μl 10 mM unbuffered Tris base and absorbance was read at 565 nm in a plate reader (SpectraMax Plus 384, Molecular Devices). A standard curve was constructed by seeding known number of cells in quadruplicate wells and fixing and staining them in parallel with sample wells. To calculate the proportion of oxygen consumption attributable to proton leak and reserve capacity, OCRs following injection of oligomycin, FCCP and rotenone/antimycin A were expressed as a percentage of the total basal OCR [42] [40].
To assess total methylglyoxal formation in cells by RP-HPLC, the cell layers were washed in PBS (1 ml), scraped into 1.5 ml PBS and pelleted by centrifugation (16,000 g for 2 min). The cell pellet was re-suspended in 500 μl KCl buffer, 500 μl 0.1% formic acid was added, protein was pelleted by centrifugation as above, and the supernatant collected. Methylglyoxal was then derivatized to 2-methylquinoxaline by addition 100 μM o-phenylenediamine followed by incubation at 37° C. for 4 h. Samples were dried under vacuum and assessed for 2-methylquinoxaline as previously described [28]. Briefly, dried samples were re-suspended in 1 ml HPLC buffer [68% (v/v) 10 mM KH2PO4, pH 2.5 and 32% acetonitrile], filtered (0.22 μm PVDF; Millex, Millipore) and separated by isocratic RP-HPLC in the HPLC buffer above at a flow rate of 2 ml/min at room temperature using a Gilson 321 pump with a C18 column (Jupiter 300 Å, Phenomenex) and a Widepore C18 guard column (Phenomenex). Peaks were detected fluorometrically (excitation and emission wavelengths of 352 and 385 nm, respectively; RF-10AXL, Shimadzu).
For LC-MS/MS analysis of MitoG reaction products cells were incubated in T25 flasks (Nunc) with 2 μM MitoG at 37° C. Cell monolayers were washed (1 ml PBS), scraped into 1.5 ml PBS and pelleted by centrifugation (16,000 g for 2 min). The pellet was re-suspended in 250 μl 100% acetonitrile/0.1% spiked with deuterated internal standards (ISs) (100 μmol each of d15-MQE and d15-QE), vortexed and centrifuged (2×16,000 g for 15 min). Samples were dried under vacuum (Savant SpeedVac), re-suspended in 100 μl 20% acetonitrile/0.1% formic acid, vortexed, centrifuged (16,000 g for 10 min), transferred to silanised autosampler vials [Chromacol #1.5HRRV(S)], flushed and sealed under argon and then stored at −80° C. until LC-MS/MS analysis. Where specified, cells were subjected to acute glycaemia treatment and were incubated either in low glucose (5 mM D-glucose), high glucose (30 mM D-glucose), or in osmotic control medium (5 mM D-glucose+25 mM L-glucose) for 4 h.
Mouse Experiments
The Akita mouse model of type I diabetes (Ins2+/−AkitaJ) [43-45] was assessed at the University of Alabama, USA. All procedures were performed in accordance with “The Guide for the Care and Use of Laboratory Animals”, and were approved by the Institutional Animal Care and Use Committee at the University of Alabama at Birmingham. Male Ins2+/−AkitaJ and wild type (C57BL/6) mice (4-8 weeks old) from Jackson Laboratory (Bar Harbor, Me., USA) were maintained on laboratory chow and water ad libitum until 14 weeks old when they were used for experiments. For this MitoG (100 nmol in 100 μl saline) was administered by tail vein injection. After 4-6 h the mice were killed and urine samples taken and snap frozen for subsequent analysis of MQE/QE content. Blood glucose levels were measured using an Accu-Chek Advantage blood glucose meter (Roche Diagnostics). Urine creatinine levels were determined at the Core Biochemical Assay Laboratory (Addenbrooke's Hospital, Cambridge, UK). To extract MQE and QE, 20 μl urine was mixed with 500 μl 60% acetonitrile/0.1% formic acid and incubated on ice for 30 min. The extracts were then spiked with deuterated ISs (100 pmol each of d15-MQE and d15-QE), vortexed for 30 sec and incubated on ice for 30 min with vortexing every 10 min and then centrifuged (16,000 g for 10 min). The supernatants were collected, filtered (0.22 m PVDF, Millex, Millipore) and dried under vacuum (Savant SpeedVac). The dried samples were re-suspended in 150 μl 20% acetonitrile/0.1% formic acid by vortexing for 5 min, followed by centrifugation at 16,000 g for 10 min. Samples were then transferred to silanised autosampler vials [Chromacol #1.5HRRV(S)], flushed and sealed under argon, and stored at −80° C. until LC-MS/MS analysis.
LC-MS/MS Analysis
The MS fragmentation patterns of TPP compounds were determined by direct infusion of compounds [1 μM in 20% acetonitrile (v/v)] at 2 μl/min into a triple quadrupole mass spectrometer (Waters Quattro Ultima). Electrospray ionisation in positive ion mode was used with the following settings: source spray voltage—3 kV; cone voltage—100 V; ion source temperature—80° C.; collision energy—50V. Nitrogen and argon were used as the curtain and collision gases, respectively.
LC-MS/MS analyses were carried out using a triple quadrupole mass spectrometer (Waters Xevo TQ-S) with an I-class Aquity LC system (Waters) attached. Samples were kept at 4° C. prior to injection by the autosampler of 10 μl into a 15 μl flow-through needle and RP-HPLC at 30° C. using a Luna 5 Phenyl-Hexyl column (1×50 mm, 5 μm; Phenomenex) with a Phenyl-Hexyl guard column (2×4 mm; Phenomenex). Buffers used were MS buffer A [0.1% (v/v) formic acid in water] and MS buffer B [95% acetonitrile/0.1% formic acid (both v/v)]. A gradient was run at 50 l/min as follows: 0-2 min—5% B; 2-3 min—5-25% B; 3-5 min—25-75% B; 5-7 min—75-100% B; 7-10 min—100% B; 10-12 min—100-5% B; 12-20 min—5% B. Eluant was diverted to waste from the mass spectrometer at 0-5 min and 16-20 min acquisition time using an in-line divert valve. For MS analysis, electrospray ionization in positive ion mode was used: source spray voltage—2.5 kV; cone voltage—25 V; ion source temperature −100° C.; collision energy—38 V. Nitrogen and argon were used as the curtain and the collision gases respectively. Multiple reaction monitoring in positive ion mode was used for compound detection. Transitions used for quantification were as follows: MQE, 506>262; d15-MQE, 521>277; QE, 492>262 and d15-QE, 507>277. For each experiment standard curves were prepared using known amounts of MQE and QE, which were spiked with IS and extracted in parallel with the samples. Standards and samples were quantified using MassLynx 4.1 software to determine the peak area for MQE, QE and ISs, and the standard curves used to determine the amount of MQE and QE present in samples.
Statistics
Data analysis was performed with R software environment for statistical computing and graphics (R Foundation for Statistical Computing, Wien, Austria). All data were analyzed using t-tests or one-way analysis of variance (ANOVA) followed by post-hoc Dunnett's test as appropriate, and represented as mean±standard error of mean (S.E.). P-values equal to or less than 0.05 were taken to be statistically significant.
Results and Discussion
Synthesis and Characterization of MitoG and its Reaction Products
The syntheses of MitoG, its predicted quinoxaline products upon reaction with methylglyoxal and glyoxal, and their deuterated versions are summarized in
The expected products of the reaction of MitoG with methylglyoxal (MQE) and glyoxal (QE) were synthesized and have UV/Vis absorption spectra distinct from that of MitoG (
Reactivity of MitoG with Glyoxal and Methylglyoxal
MitoG reacted with methylglyoxal and glyoxal to form products with identical absorption, fluorescence and RP-HPLC properties (
Progress of the reaction between MitoG and methylglyoxal could be observed by UV/Vis spectrophotometry, but that of MitoG with glyoxal was too slow (
Accumulation of MitoG within Energized Mitochondria
To be a mitochondria-selective probe for 1,2-dicarbonyls in cells and in vivo, the TPP moiety of MitoG should promote its uptake within mitochondria. The uptake of MitoG by isolated mitochondria using an electrode selective for the TPP-moiety of MitoG was measured (
For MitoG to be useful, it should not disrupt mitochondrial function or cause cell toxicity at the concentrations used. To test this MitoG was incubated with isolated mitochondria and assessed its effect on respiration under different conditions. At 5 μM MitoG there was a slight increase in proton leak as indicated by an increase in coupled respiration (
Quantification of Reaction of MitoG with 1,2-Dicarbonyls by LC-MS/MS
To use MitoG to probe the local concentration of methylglyoxal and glyoxal, it was necessary to measure the amounts of the reaction products (MQE, QE) by LC-MS/MS relative to deuterated internal standards [31]. The fragmentations of MQE and QE and their deuterated versions during tandem MS were determined (
MitoG as Probe for Mitochondrial Methylglyoxal and Glyoxal in Cells
For MitoG to be an effective probe it should react with methylglyoxal and glyoxal within a biological system to give the diagnostic products, MQE and QE, which can then be extracted and analysed by LC-MS/MS. To assess whether this was possible in cells, BAECs were preincubated with MitoG for 1 h, then methylglyoxal or glyoxal was added and after a further 3 h the cell layers were extracted and analysed by LC-MS/MS to assess the amounts of MQE (
Next MitoG was utilized to determine relative mitochondrial levels of methylglyoxal and glyoxal under hyperglycaemia, a condition in which damaging glycation by 1,2 dicarbonyls is thought to contribute. First it was confirmed that hyperglycaemia did increase the production of cellular methylglyoxal in our system. To do this BAEC cells were incubated under conditions of high (30 mM) and low (5 mM) glucose for 4 h and then measured the formation of methylglyoxal by derivitization with o-phenylenediamine to generate 2-methylquinoxaline, which was assessed by RP-HPLC. This analysis showed that hyperglycaemia in BAEC did indeed increase the formation of methylglyoxal ˜2-fold compared to controls (data not shown, n=3). To see whether MitoG could assess a change in mitochondrial methylglyoxal/glyoxal under hyperglycemic conditions the formation of MQE and QE was next compared in cells following incubation with low (5 mM) glucose or high (25 mM) glucose for 4 h (
When BAECs were incubated under conditions of high glucose in the presence of the glyoxalase I inhibitor bromobenzyl glutathione cyclopentyl diester [35], to suppress degradation of glyoxal and methyl glyoxal, the amount of MQE and QE increased (
MitoG as a Probe for Mitochondrial Levels of Methylglyoxal and Glyoxal In Vivo
Previously it has been shown that the mitochondria-targeted hydrogen peroxide mass spectrometry probe MitoB can be used to assess the production of hydrogen peroxide within mitochondria in living fruit flies [30, 31] and mice [50]. In this, MitoB is acting as a probe to generate the exomarker MitoP [51], that is an exogenous probe compound that when administered to an experimental animal is converted to a diagnostic marker that can be assessed ex vivo and used to infer the production of reactive species within the living organism [51]. Therefore next a study was carried out to see if MitoG could also be used to generate the exomarkers MQE and QE so as to assess the formation of methylglyoxal/glyoxal within mitochondria in vivo. The TPP component of MitoG facilitates this goal, as it is known that following intravenous injection TPP compounds rapidly distribute from the blood to mitochondria within tissues and are then slowly excreted over several hours into the urine and bile [27, 52, 53]. Therefore it may be possible to administer MitoG to mice and then analyze urine to see if there is elevated production of the MQE/QE products under certain conditions, indicating an elevation in reactive dicarbonyls within mitochondria in vivo.
To do this the Akita mouse model (Ins2+/−AkitaJ) was used in which a mutation in proinsulin leads to chronic hyperglycaemia and consequent pathological complications similar to those found in type I diabetes. [43-45]. To assess whether there were changes in mitochondrial methylglyoxal/glyoxal in the Akita mice compared to wild type, MitoG (100 nmol) was administered as a tail vein injection and after 4-6 h urine samples were isolated and the MQE and QE contents measured relative to creatinine. These data are shown as a function of blood glucose levels for individual mice (
Stability of MitoG
MitoG 100 μM was mixed with 0.07 U horse-radish peroxidase (HRP) in a 1 ml curvette containing 100 mM potassium phosphate buffer (pH 6.0) and 0.5% (w/v) hydrogen peroxide at room temperature. A reference curvette and a blank were similarly set up with DMSO in place of MitoG. Scanning UV/Vis spectra were then taken at 1 min, 5 min and 10 min after mixing using a spectrophotometer (Shimadzu UV-2501PC). Spectra of MitoG-containing mixtures were compared against the spectrum of the blank. To determine if any reaction observed could be prevented by the HRP inhibitor, NaN3 [58, 59] separate incubations were set up as above but containing 8 mg/ml NaN3 as well.
Oxidative degradation of o-phenylenediamine is known to result in the production of methylglyoxal and glyoxal [60]. This oxidative degradation can lead to an overestimation of dicarbonyl levels in samples if o-phenylenediamine is used to try to quantify methylglyoxal and glyoxal levels in biological samples. To determine if MitoG could undergo similar oxidative degradation to methylglyoxal and glyoxal, MitoG was incubated with HRP and hydrogen peroxide in vitro. Scanning UV/Vis spectra of the reaction mixture were taken before and after the addition of HRP. HRP was used as a representative peroxidase that could catalyse an oxidative degradation in a cellular environment. As shown in
Based upon the differences in the absorption profile (compare
Protection by MitoG Against Dicarbonyl-Induced Respiratory Depression and Cell Death
Isolated RLM respiring on glutamate/malate were incubated with MitoG, methoxyphenylenediamine (MP) or butylTPP for 2 min, followed by 0.5 mM methylglyoxal or 5 mM glyoxal for 5 min. ADP was then added and the rate of oxygen consumption monitored at 37° C. (see
To evaluate the usefulness of a mitochondria-targeted approach against glycation-induced toxicity, oxygen consumption by isolated RLM that had been treated with MitoG prior to α,β-dicarbonyl exposure was measured. As shown in
Hence, it has been shown that MitoG ameliorates methylglyoxal-induced respiratory depression, and both methylglyoxal- and glyoxal induced cytotoxicity. Non-targeted derivatives of phenylenediamine, MP and HP, were not significantly protective in both isolated mitochondria and cell cultures. Thus, protection by MitoG appears to be related to specific mitochondrial scavenging of these reactive carbonyl species.
Study of the Effects of MitoG-Amide on Diabetic Complications in the Heart
To determine the effects of MitoG-amide on diabetic complications in the heart a well-established mouse model of type I diabetes was used, based on destruction of the beta-cells in the pancreas by administration of the beta-cell toxin streptozotocin (STZ; obtainable from Selleck Chemicals, Munich, Germany). Mice that were 6-7 week old C57Bl6/J mice (>20 g) were administered STZ (50 mg/kg by intraperitoneal injection) on 5 consecutive days. The death of the beta-cells and the induction of the high blood glucose associated with Type I diabetes was confirmed by weekly blood glucose tests on mice tail vein blood sample. A blood glucose level >17 mmol/l was considered diabetic. For experiments, mice were divided into three groups: (a) control; (b) STZ-induced Type I diabetes; and (c) STZ-induced Type I diabetic animals treated with MitoG-amide (10 mg/kg body weight once per day via gavage). This was repeated for 12 weeks (
For the cardiac magnetic resonance imaging (MRI) measurement a standard cardiac MRI protocol with a 4.7T Bruker BioSpec system was performed 24 h after surgery. Anaesthesia was induced with 3% and maintained with 1.25% isoflurane in oxygen. Temperature was monitored with a rectal probe and maintained constant via a heated water blanket, respiration was monitored using a pillow connected to a piezoelectric transducer. Electrocardiogram (ECG) signals were monitored with neonatal graphite (3M) electrodes, placed over the front left and rear right paws.
A 12 cm diameter birdcage was used to transmit the signal and a 4 channel cardiac array coil was used for signal reception. The imaging protocol consisted of scout scans followed by ECG-gated fast imaging with steady state precision (FISP) slices (repetition time/echo time TR/TE 6/2.4 ms 13-20 frames, 3.5 cm field of view (FOV), 256 matrix, 1 mm slice thickness, bandwidth 64.1 kHz, flip angle (FA) 20°, 2 sampling average [number of excitations, NEX]), in the long-axis and in the short-axis to cover the whole heart. In post processing, volumes during different phases of the ECG were delineated and integrated over whole heart using Simpson's rule. Global functional parameters were obtained including ejection fraction, the fraction of blood ejected into the circulation during one heartbeat.
For the Pressure/Volume (P/V) measurement, left ventricular catheterization via the right carotid artery was carried out using a 1.2 F pressure-catheter (Scisense Inc., London, Canada).
The results of the long-term treatment of the STZ-induced Type I diabetes administered with MitoGamide on body weight, blood glucose and heart rate were compared with placebo-treated STZ mice and controls (
Study of the Effects of MitoG-Amide on Hyperglycaemia in Type I Diabetes
To assess the effect of MitoGamide on the well-known detrimental effects of long-term hyperglycaemia in Type I diabetes heart function was measured by MRI and by P/V loop measurement (
The systolic ejection fraction and the end diastolic volume were measured with MRI according to the procedures given in “Functional assessment of the mouse heart by MRI with a 1-min acquisition”, Buonincontri, G.; Methner, C.; Krieg, T.; Carpenter, T. A. and Sawiak, S. J.; NMR Biomed (2014), Vol. 27; pages 733-7.
The left ventricular diastolic time constant (Tau Weiss) and the end diastolic pressure were measured from the P/V loop system using a Transonic ADV500 with the corresponding software LifeScribe2.
The diastolic stiffness was measured from the MRI-corrected P/V loops.
From this it was found that neither STZ nor MitoGamide had any effect on systolic ejection fraction. As expected, STZ treatment impaired end diastolic volume and the left ventricular diastolic time constant (Tau Weiss) but these were not ameliorated by MitoGamide (
Finally, at the end of the analysis, hearts were excised and fixed with formalin and processed for histology and then cut into 10 m slices and Optimal Cutting Temperature (OCT) Embedded. To assess oxidative damage to the tissue an antibody against 4-hydroxynonenal, a marker of oxidative damage, (antibody ab48506 HNE-J-2, 1:5 in blocking buffer; obtainable from Abcam plc) was used. These experiments were performed as described in Kaludercic, N.; et al. (2014) “Monoamine oxidase B prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts” Antioxidants & Redox Signaling, Vol. 20, pages 267-80, with the exception that 2nd AB Goat anti-mouse (Invitrogen F2761 available from Thermo Fischer Scientific Inc.)+fluorescein (molecular probes, obtainable from Sigma-Aldrich) and 10 M Hoechst stain 33342 (Sigma B2261 obtainable from Sigma-Aldrich) at room temperature was used as the secondary antibody solution.
The extent of staining was quantified and the data are presented in
Disruption to mitochondrial function has been proposed to play a role in pathologies associated with hyperglycaemia. One plausible pathway by which this may occur is through damaging glycation reactions to mitochondrial components by the reactive 1,2-dicarbonyls methylglyoxal and glyoxal. However the importance of this pathway was difficult to assess in cells and in vivo due to the uncertainties about the levels of methylglyoxal and glyoxal that occur within mitochondria during hyperglycemia. To alleviate these problems a mass spectrometry approach has been developed to measure changes in the levels of these reactive 1,2-dicarbonyls within mitochondria in cells and in vivo.
In particular, it has been found that the combination of an o-phenylenediamine with a TPP moiety led to a molecule that accumulated within mitochondria where it reacted with methylglyoxal and glyoxal to form stable products. These products could then be extracted from cells and biological fluids and analyzed by LC/MS/MS relative to stable isotope internal standards. The selectivity of this assessment for methylglyoxal and glyoxal was based on the identification of the products by tandem mass spectrometry, and this was further demonstrated by the changes in their levels on pharmacological inhibition of the glyoxalase system and by addition of the dicarbonyl trap aminoguanidine. The ability of the TPP moiety to locate compounds to mitochondria in cells and in vivo is well established, suggesting that the formation of MQE/QE occurs predominantly due to the reaction with methylglyoxal/glyoxal within the mitochondrial matrix. This is supported by the inhibition of the formation of MQE/QE by addition of the uncoupler FCCP, which decreases the uptake of MitoG into mitochondria. Furthermore, as the reaction between MitoG and methylgloxal/glyoxal is second order, the ˜1,000-fold concentration of MitoG within mitochondria will mean that the rate of formation of MQE/QE will be greater inside mitochondria by a similar factor. Thus formation of MQE/QE within a cell or tissue is largely due to changes in the amount of methylglyoxal/glyoxal within mitochondria. However, other reactive species within biological systems such as nitric oxide or reactive aldehydes such as 4-hydroxynonenal may also react with and deplete MitoG, although these reactions will not produce the diagnostic products formed from the reaction with glyoxal/methylglyoxal. Furthermore, while this approach will report on mitochondrial dicarbonyl exposure, it does not indicate the cellular source of the methylglyoxal/glyoxal, and they may be largely produced in the cytosol with subsequent diffusion into mitochondria.
The ability to use MitoG within living mice to generate MQE/QE as exomarkers to assess the changes in dicarbonyls that occurred within a model of type I diabetes was an important step. Hence, this approach will be useful in assessing the role of reactive dicarbonyls in the mitochondrial damage associated with diabetes and in developing specific therapies.
The findings in
The results shown in
All publications mentioned in the above specification are herein incorporated by reference. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in chemistry or related fields are intended to be within the scope of the following claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1320636.2 | Nov 2013 | GB | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2014/075323 | 11/21/2014 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2015/075200 | 5/28/2015 | WO | A |
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| Number | Date | Country | |
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| 20160289252 A1 | Oct 2016 | US |
