Patent Application
20140088197
The present invention relates to a mixed crystalline form of agomelatine, N-[2-(7-methoxy-1-naphthyl)ethyl]acetamide, its method of preparation, application and pharmaceutical composition.
Agomelatine, with chemical name N-[2-(7-methoxy-1-naphthyl)ethyl]acetamide and brand name Valdoxan, has the following chemical structure:
It has a dual effect, acting not only as an agonist of melatoninergic system receptors, but also as an antagonist of the 5HT2C receptor. Its properties mean that it is active in the central nervous system, especially in the treatment of severe depression, seasonal affective disorder, sleep disorders, cardiovascular diseases, digestive system diseases, insomnia and fatigue brought on by jet lag, eating disorders and obesity. Agomelatine is the first melatoninergic antidepressant, and is effective in the treatment of depression and the improvement of sleep parameters, while not affecting sexual function.
The preparation and therapeutic use of agomelatine have been reported in the European patent EP0447285.
In view of the pharmaceutical value of said compound, it is important to obtain a highly pure, stable crystalline form with good reproducibility in order for it to be advantageous in pharmaceutical preparation and stable enough for long-term storage without having specific requirements in terms of temperature, light, humidity or oxygen levels.
The Chinese patents CN200510071611.6, CN200610108396.7, CN200610108394.8, CN200610108395.2, CN200910047329.2, CN200910245029.5 have made public the various crystalline forms as well as the preparation methods of agomelatine.
Among these, in CN200910047329.2, the publicly disclosed crystalline form VI obtained through the process of acetic acid and water recrystallization has superior solubility over most of the existing publicly disclosed crystalline forms, thus possessing unique value with regard to its properties in pharmaceutical formulation. However, when placing crystalline form VI under extreme conditions (high temperature of 60° C.) for 10 days, small amounts of crystal transition can occur.
Researchers have focused on the search for a crystalline or mixed crystalline form possessing greater stability without compromising in terms of solubility.
Advantageously, through exploring various preparation processes and comparisons of stability, the inventor has found a mixed crystalline form which, when placed under extreme conditions, offers superior stability over crystalline form VI. Said mixed crystal achieves greater stability without compromising the excellent solubility of crystalline form VI. It offers great reproducibility and under extreme conditions, stability in its preparation process, thus greatly increasing the feasibility of pharmaceutical formulation.
The aim of the present invention is to provide a mixed crystalline form of agomelatine, form VIII, while also providing a preparation process. When compared with crystalline form VI, said form VIII offers greater stability under high temperature. In addition, it exhibits valuable pharmaceutical formulation properties.
The mixed crystalline form VIII of agomelatine in the present invention may be used in the treatment of diseases of the melatoninergic system, sleep disorders, stress, anxiety, seasonal affective disorder, severe depression, cardiovascular diseases, digestive system diseases, insomnia and fatigue brought on by jet lag, schizophrenia, phobias, and depression.
The present invention also aims to provide a method of preparation for form VIII of agomelatine which is simple in its operation and offers good reproducibility.
A further aim of the present invention is to provide a pharmaceutical composition, which includes the mixed crystalline form VIII of agomelatine of this invention as well as pharmaceutically acceptable adjuvants or excipients.
The said pharmaceutical composition can be configured to be used in different application routes, especially when administered either orally or via injection.
According to the nature and severity of the illness, treatment may be administered via a regulated dosage based on the age and weight of the patient. The dosage may vary between 0.1 mg and 1 g per day, being administered once only or several times.
The following examples of X-ray diffraction diagrams of form VIII of agomelatine of the present invention use interplanar crystal spacing d, Bragg angle 2θ and relative intensity (I%) to show:
When using X-ray diffraction to measure the crystallization of the present invention, sometimes owing to the measurement equipment or test conditions, the measured peaks show slight deviations in measurement; more specifically, for example there may be a deviation in measurement of the 2θ value by approximately ±0.2; even if extremely accurate equipment is used, a deviation of approximately ±0.1 may be seen. As a result, this deviation must be taken into consideration when determining each crystalline structure.
XRD test conditions for said form VIII of agomelatine of the present invention:
Instrument model: Bruker D8 ADVANCE X-ray diffractometer
Experiment parameters:
The onset value of the endothermic peak of the DSC change-in-absorption diagram of the present invention is characterised by: onset value range being 97-98° C., the endothermic peak area being no lower than 90%, with the preferable ratio being 95-99%.
When using DSC to measure the crystals of the present invention, sometimes owing to the measurement equipment or test conditions, the measured peaks show slight deviations in measurement; more specifically, for example there may be a deviation in measurement of the onset value by approximately ±1° C., even if extremely accurate equipment is used, a deviation of approximately ±0.5° C. may be seen. As a result, this deviation must be taken into consideration when determining each crystalline structure.
TGA test conditions of the present invention:
Instrument model NETZSCH TG 209F1
Experimental conditions:
The method of preparation of form VIII of the present invention involves dissolving agomelatine compound of formula (II) (Agomelatine-HCl-H2O) in acetic acid, to which sodium acetate is then added, water is then added dropwise to this reaction mixture and agitated at a temperature of 7-13° C. in order to bring about crystallization, with the crystals then being separated from the solution.
In the present invention as described, there are no special requirements in terms of the amount of acetic acid that is to be added as long as a sufficient amount is used to dissolve the raw materials, while heating can also be suitably applied to facilitate dissolution.
The molar ratio of agomelatine compound of formula (II) and sodium acetate is preferably of the order of 1:1-1.5, most optimally 1:1-1.1.
In the preparation method of the present invention as described, the ratio of volume of acetic acid to water is 1:15-30.
In a preferred embodiment of the preparation method for agomelatine form VIII in the present invention, when the temperature of the resulting reaction mixture reaches 12-18° C., and in particular when around 15° C., water is added dropwise in order to bring about crystallization.
In a further preferred embodiment, when water is added dropwise to the resulting reaction mixture, agitation is then carried out at a temperature of around 10° C. This may be carried out over a period of around 1.5 hours in order to bring about crystallization.
In another preferred embodiment, following the addition of sodium acetate, the reaction mixture is heated to 40-80° C., an appropriate, non-fixed, amount of activated carbon is then added, followed by agitation and filtration; said solution is then left to cool on its own, and water is added dropwise in order to bring about crystallization.
The agomelatine form VIII provided by the present invention can be used in conjunction with pharmaceutically acceptable adjuvants or excipients for pharmaceutical formulation.
The present invention results in a new form VIII of agomelatine, with greater stability compared to that of crystalline form VI, thus possessing advantages in production in terms of stability.
According to the Chinese patent application CN 201010126254.X, agomelatine compound of formula (II) as previously described may be produced by means of the following preparation method, where said preparation method involves reacting agomelatine with various forms of HCl in order to form a hydrate. The two methods are as follows: Agomelatine is firstly dissolved in a water-containing organic solvent, after which HCl gas is added, the solid crystals are washed and then dried; or else agomelatine is added to a solvent containing HCl, and the solid crystals are then washed and dried. If the first method is used, an overabundance of HCl may lead to a decrease in yield, while in the second method the amount of HCl present in the solvent is easily controlled. Therefore the second method is preferred.
Specifically, agomelatine may be added to a water-containing organic solvent, followed by the dropwise addition of a solvent containing HCl. The solid crystals are then washed and then dried.
Likewise, it is also possible to add agomelatine to an organic solvent, followed by the dropwise addition of an aqueous solution containing HCl. The solid crystals are then washed and then dried. The full contents of reference documents either quoted or mentioned in this application have been referenced.
14 g of agomelatine compound of formula (II) is dissolved in 55 ml of acetic acid, to which 4.5 g of sodium acetate is then added; the mixture is then heated to 60° C., after which 0.5 g of activated carbon is added. Agitation is carried out for 2 hours after which the mixture is filtered; at a temperature of 15° C., 1 L of water is then added dropwise. The solution gradually becomes turbid, and at a temperature of ˜10° C., agitation is carried out over 1.5 hours, followed by filtration, then washing and drying the filter cake at 45° C. under vacuum until constant weight is achieved, resulting in 9.6 g of white solid;
(Refer to
140 g of agomelatine compound of formula (II) is dissolved in 490 ml of acetic acid, to which 60 g of sodium acetate is then added; the mixture is then heated to 60° C., after which 1.4 g of activated carbon is added. Agitation is carried out for 1 hour after which the mixture is filtered; at a temperature of 15° C., 8.8 L of water is then added dropwise. The solution gradually becomes turbid, and at a temperature of ˜10° C., agitation is carried out over 1.5 hours, followed by filtration, then washing and drying the filter cake at 45° C. under vacuum until constant weight is achieved, resulting in 94 g of white solid;
(Refer to
66 g of agomelatine compound of formula (II) is dissolved in 230 ml of acetic acid, to which 21 g of sodium acetate is then added; the mixture is then heated to 60° C., after which 1.3 g of activated carbon is added. Agitation is carried out for 1 hour after which the mixture is filtered; at a temperature of 15° C., 6.9 L of water is then added dropwise. The solution gradually becomes turbid, and at a temperature of ˜10° C., agitation is carried out over 1.5 hours, followed by filtration, then washing and drying the filter cake at 50° C. under vacuum until constant weight is achieved, resulting in 49 g of white solid;
(Refer to
Agomelatine crystalline forms VI and VIII (obtained through embodiment 2) are each placed in thermostatic containers at a temperature of 40° C. and stored for 20 days, with the stability of these samples being studied using the method of High Performance Liquid Chromatography.
1. Purity Measurement of the Sample
Chromatographic conditions: Octadecyl silane chemically bonded silica is used as packing; a mixed solution of 10 mmol/L phosphate buffer (adjusted to pH 7.0 with sodium hydroxide) and acetonitrile in the ratio 2:7 by volume acts as the mobile phase; column temperature 40° C.; and detection wavelength 220 nm. Purity is measured using an internal standard method.
In the mobile phase, crystalline forms VI and VIII are distributed into 1 mg/mL solutions, 10 μL of each of which are then passed into a liquid chromatograph, with their chromatograms being recorded.
The reference sample purity measurement method was used, with measurements being made using an external standard method, the results can be seen in Table I.
The HPLC method was used to determine water solubility, with measurements being made using an external standard method. The results are shown in Table II.
Measured using the pharmacopoeia stability assessment method:
As can be seen from the test results, form VIII of agomelatine of the present invention clearly offers greater stability under high temperature and comparable solubility when compared with crystalline form VI. Its preparation method offers good reproducibility. In addition, it exhibits valuable pharmaceutical formulation properties.
5. Study into the Preparation and Stability of Pharmaceutical Compositions (Crystalline Form, Purity and Content)
Subjected to the pharmacopoeia stability assessment method and undergoing influencing factor testing (10 day exposure): High temperature (60° C.), illumination (4500 1×), high humidity (92.5% RH, 25° C.); Accelerated testing (hermetically sealed for 6 months): temperature 30° C., humidity 65% RH, Long term testing (hermetically sealed for 12 months): temperature 25° C., humidity 60% RH. The assessment results demonstrate that under the above conditions neither the crystalline form, purity nor content of the product underwent any changes.
Consequently, the test results of the pharmaceutical ingredients and capsules of this product show that form VIII has a great potential in pharmaceutical production.
10 g of agomelatine is added to a 100 ml solution of ethyl acetate. At a temperature of 10° C., 4.6 g of an aqueous solution of HCl (36%) is slowly added dropwise. Agitation is then carried out for 1 hour, followed by filtration and the resulting solid is washed twice in 10 ml of ethyl acetate, then dried at a temperature of 40° C. to obtain 10.2 g of form II white solid; purity: 99.8%, yield: 88.7%.
Cl elemental analysis:
Determination of crystal water content of agomelatine compound of formula (II):
The calculated theoretical crystal water content of C15H17NO2.HCl.H2O is 6.06 wt %.
The product resulting from embodiment 5 was measured according to the Fischer method as mentioned above, and the crystal water content recorded was: 6.15 wt %.
The product resulting from embodiment 5 was measured according to thermogravimetric analysis as mentioned above, and the loss of crystal water recorded was: 6.67 wt %, i.e. the crystal water content of the original product was 6.67 wt %. For TGA curve, please refer to
| Number | Date | Country | Kind |
|---|---|---|---|
| 201110070634.0 | Mar 2011 | CN | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/CN12/72818 | 3/22/2012 | WO | 00 | 9/20/2013 |
