Claims
- 1. A method of preliminarily screening peptides for immunogenicity comprising the steps of:
1) creating a molecular mode of receptor DR1 Class II MHC and minimizing the model of the DR1; 2) modeling a peptide to be tested and minimizing the model of the peptide; and 3) testing fit of model obtained in step 2 into the model obtained in step 1 to produce composite receptor/peptide model.
- 2. A computerized model comprising a model of the DR1 molecule having fitted in a cleft therein a model of a peptide.
- 3. A method of claim 1 wherein, additionally, the receptor/peptide model is subjected to computer-simulated heating.
- 4. A method of claim 1 further comprising, assaying the peptide by competitive inhibition binding to a Class II MHC receptor DR1.
- 5. A minimize peptide capable of binding to a Class II MHC receptor DR1 an inhibiting the binding of HA (306-318).
- 6. A synthetic peptide, wherein the amino acid sequence of the minimized peptide according to claim 5 has been modified to have a superior binding affinity for a Class II MHC receptor DR1 to form at least a portion of the synthetic peptide.
- 7. A synthetic peptide, wherein the amino acid sequence of the minimized peptide according to claim 5, has been modified to have greater inhibition of HA (306-318) binding to a Class II MHC receptor DR1 to form at least a portion of the synthetic peptide.
- 8. A synthetic peptide according to claim 6, wherein an amino acid has been modified from a charge amino acid to an uncharged amino acid.
- 9. A synthetic peptide according to claim 7, wherein an amino acid has been modified from a charged amino acid to an uncharged amino acid.
- 10. A synthetic peptide according to claim 8, wherein said uncharged amino acid is alanine.
- 11. A synthetic peptide according to claim 9, wherein said uncharged amino acid is alanine.
- 12. A minimized peptide according to claim 5, wherein the sequence is selected from the group consisting of PKYVKQNTLKLAT, AAYAAAAAAKAA and SKNGTVTWAHETNNSA, SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3 respectively.
- 13. A minimized peptide according to claim 5, wherein the sequence is contained in a CFA.
- 14. A minimized peptide according to claim 13, wherein the sequence is selected from the group consisting of DEYGLGRLVNTAD, IIYQIVDEKGKKK, LNYTSGEKKISPG, WQYKSLDVNVNIE, QLTVEMTIPAGV, TSYTFSAIYTGGE, GEYPNSGYSSGTY and GTYAGHLTVSFYS, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12 respectively.
- 15. A vaccine comprising:
a minimzed peptide according to claim 5; and an immunologically acceptable carrier.
- 16. A vaccine comprising:
a synthetic peptide according to claim 6; and an immunologically acceptable carrier.
- 17. A vaccine comprising:
a synthetic peptide according to claim 7; and an immunologically acceptable carrier.
- 18. A method of eliciting an immune response in an animal comprising administering said animal with the vaccine according to claim 15.
- 19. A method of eliciting an immune response in an animal comprising administering said animal with the vaccine according to claim 16.
- 20. A method of eliciting an immune response in an animal comprising administering said animal with the vaccine according to claim 17.
CROSS REFERENCE
[0001] This application is a continuation in part of U.S. patent application Ser. No. 08/789,734 filed Jan. 27, 1998 which in turn is a continuation in part of U.S. patent applicaton Ser. No. 08/590,973 filed Jan. 24, 1996 which in turn is a continuation in part of U.S. patent application Ser. No. 08/247,884 filed May 23, 1994 which in turn is a continuation-in-part of U.S. patent application Ser. No. 08/064,559, filed May 21, 1993, and present application incorporates U.S. patent application Ser. Nos. 08/064,559, 08/789,734, 08/590,973 and 08/247,884 in their entirety by reference.
GOVERNMENT INTEREST
[0002] The invention described herein may be manufactured, licensed and used by or for governmental purposes without the payment of any royalties to us thereon.
Continuation in Parts (4)
|
Number |
Date |
Country |
Parent |
08789734 |
Jan 1997 |
US |
Child |
09013077 |
Jan 1998 |
US |
Parent |
08590973 |
Jan 1996 |
US |
Child |
08789734 |
Jan 1997 |
US |
Parent |
08247884 |
Oct 1995 |
US |
Child |
08590973 |
Jan 1996 |
US |
Parent |
08064559 |
May 1993 |
US |
Child |
08247884 |
Oct 1995 |
US |