Claims
- 1. A method for manipulating the metabolism of a plant, comprising expressing heterologous genes encoding fatty acid oxidation enzymes in the cytosol or plastids other than the peroxisomes, glyoxisomes or mitochondria, of the plant.
- 2. The method of claim 1 wherein the fatty acid β-oxidation enzymes are expressed from genes from selected from the group consisting of bacterial, yeast, fungal, plant, and mammalian genes.
- 3. The method of claim 2 wherein the fatty acid oxidation enzymes are expressed from genes from bacteria selected from the group consisting of Escherichia, Pseudomonas, Alcaligenes, and Coryneform.
- 4. The method claim 3 wherein the genes are Pseudomonas putida faoAB.
- 5. The method of claim 1 further comprising
expressing genes encoding enzymes selected from the group consisting of polyhydroxyalkanoate synthases, acetoacetyl-CoA reductases, β-ketoacyl-CoA thiolases, and enoyl-CoA hydratases.
- 6. A DNA construct for use in a method of manipulating the metabolism of a plant cell comprising, in phase,
(a) a promoter region functional in a plant; (b) a structural DNA sequence encoding at least one fatty acid oxidation enzyme activity; and (c) a 3′ nontranslated region of a gene naturally expressed in a plant, wherein the nontranslated region encodes a signal sequence for polyadenylation of mRNA.
- 7. The DNA construct of claim 6 wherein the promoter is a seed specific promoter.
- 8. The DNA construct of claim 7 wherein the seed specific promoter is selected from the group consisting of napin promoter, phaseolin promoter, oleosin promoter, 2S albumin promoter, zein promoter, β-conglycinin promoter, acyl-carrier protein promoter, and fatty acid desaturase promoter.
- 9. The DNA construct of claim 6 wherein the promoter is a constitutive promoter.
- 10. The DNA construct of claim 6 wherein the promoter is selected from the group consisting of CaMV 35S promoter, enhanced CaMV 35S promoter, and ubiquitin promoter.
- 11. A method for enhancing the biological production of polyhydroxyalkanoates in a transgenic plant, comprising expressing genes encoding heterologous fatty acid oxidation enzymes in cytosol or plastids other than the peroxisomes, glyoxisomes or mitochondria, of the plant.
- 12. The method of claim 11 wherein the transgenic plant is selected from the group consisting of Brassica, maize, soybean, cottonseed, sunflower, palm, coconut, safflower, peanut, mustards, flax, tobacco, and alfalfa.
- 13. A transgenic plant or part thereof comprising heterologous genes encoding fatty acid oxidation enzymes in cytosol or plastids other than the peroxisomes, glyoxisomes or mitochondria of the plant.
- 14. The plant or part thereof of claim 13 wherein the fatty acid β-oxidation enzymes are expressed from genes selected from the group consisting of bacterial, yeast, fungal, plant, and mammalian.
- 15. The plant or part thereof of claim 14 wherein the fatty acid oxidation enzymes are expressed from genes from bacteria selected from the group consisting of Escherichia, Pseudomonas, Alcaligenes, and Coryneform.
- 16. The plant or part thereof of claim 15 wherein the genes are Pseudomonas putida faoAB.
- 17. The plant or part thereof of claim 13 further comprising genes encoding enzymes selected from the group consisting of polyhydroxyalkanoate synthases, acetoacetyl-CoA reductases, β-ketoacyl-CoA thiolases, and enoyl-CoA hydratases.
- 18. The plant or part thereof of claim 13 wherein the plant is selected from the group consisting of Brassica, maize, soybean, cottonseed, sunflower, palm, coconut, safflower, peanut, mustards, flax, tobacco, and alfalfa.
- 19. The plant or part thereof of claim 13 comprising a DNA construct comprising, in phase,
(a) a promoter region functional in a plant; (b) a structural DNA sequence encoding at least one fatty acid oxidation enzyme activity; and (c) a 3′ nontranslated region of a gene naturally expressed in a plant, wherein the nontranslated region encodes a signal sequence for polyadenylation of mRNA.
- 20. The plant or part thereof of claim 19 wherein the promoter is a seed specific promoter.
- 21. The plant or part thereof of claim 20 wherein the seed specific promoter is selected from the group consisting of napin promoter, phaseolin promoter, oleosin promoter, 2S albumin promoter, zein promoter, β-conglycinin promoter, acyl-carrier protein promoter, and fatty acid desaturase promoter.
- 22. The plant or part thereof of claim 19 wherein the promoter is a constitutive promoter.
- 23. The plant or part thereof of claim 19 wherein the promoter is selected from the group consisting of CaMV 35S promoter, enhanced CaMV 35S promoter, and ubiquitin promoter.
- 24. A method of preventing or suppressing seed production in a plant, comprising
expressing heterologous genes encoding fatty acid oxidation enzymes in cytosol or plastids other than the peroxisomes, glyoxisomes or mitochondria, of the plant.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] Priority is claimed to U.S. application Ser. No. 09/263,406, filed Mar. 5, 1999, which claims priority to U.S. Provisional application Serial No. 60/077,107, filed Mar. 6, 1998.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60077107 |
Mar 1998 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09389395 |
Sep 1999 |
US |
Child |
10447515 |
May 2003 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09263406 |
Mar 1999 |
US |
Child |
09389395 |
Sep 1999 |
US |