Claims
- 1. A method for producing a modified 7S legume seed storage protein in a plant cell which comprises the steps of
- (a) modifying a DNA sequence of a structural gene encoding a 7S legume seed storage protein, wherein said structural gene comprises a conserved region corresponding to the conserved region of a phaseolin gene that flanks an XbaI site, by inserting a nucleotide sequence comprising a methionine codon into said conserved region;
- (b) transforming a plant cell with the modified 7S legume seed protein structural gene such that the modified structural gene is expressible in a plant cell; and
- (c) regenerating a plant descended from the transformed plant cell, whereby a plant that expresses the modified 7S legume seed storage protein structural gene is produced.
- 2. A plant produced by the method of claim 1.
- 3. A seed of a plant of claim 2 which comprises said DNA sequence encoding the modified 7S legume seed storage protein.
- 4. Progeny of the plant of claim 2 which comprises said DNA sequence encoding the modified 7S legume seed storage protein.
Parent Case Info
This is a division of application Ser. No. 07/626,682 pending, filed Dec. 12, 1990, which is a divisional of U.S. patent application Ser. No. 06/902,223, filed Aug. 29, 1986, now U.S. Pat. No. 5,003,045, which is hereby incorporated by reference.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4771002 |
Gelvin |
Sep 1988 |
|
4886878 |
Larkins et al. |
Dec 1989 |
|
Non-Patent Literature Citations (14)
Entry |
Hoffman et al 1988 Pl Molec Biol 11:717-729. |
Pedersen et al 1986 (15 May) J Biol Chem 261:6279-6284. |
Seugupta-Gopalan 1985 (May) Proc Natl Acad Sci USA 82:3320-3324. |
Schuler et al 1982 Nucleic Acids Res 10:8245-8260. |
Schuler et al 1982 Nucleic Acids Res 10:8225-8244. |
Doyle et al 1986 (May) J Biol Chem 261(20):9228-9238. |
Argos et al 1985 (May) The EMBO Journal 4:1111-1117. |
Cramer et al 1985 (Jan.) Proc Natl Acad Sci USA 82:334-338. |
Cray et al 1982 Nature 295:76-78. |
Slighton et al 1985 (Sep.) Nucleic Acids Res 13:6483-6498. |
Slighton et al 1983 Proc Natl Acad Sci USA 80:1897-1901. |
Larkins et al 1983 In Genetic Eng of Plants, an Agric Perspective, pp. 93-118. |
Nielsen 1985 (Jun.) In World Soybean Res Conf III: Proc.; Shibles ed. pp. 281-290. |
Goodman et al 1985 (Jun) In World Soybean Res Conf III: Proc.; Shibles ed. pp. 261-271. |
Divisions (2)
|
Number |
Date |
Country |
Parent |
626682 |
Dec 1990 |
|
Parent |
902223 |
Aug 1986 |
|