Claims
- 1. A human ciliary neurotrophic factor (CNTF) protein (SEQ ID NO:1) in which glutamine at position 63 (Q63) is substituted by arginine and cysteine at position 17 (C17) is substituted with another amino acid.
- 2. The protein of claim 1, wherein C17 is substituted by serine or alanine.
- 3. A human ciliary neurotrophic factor (CNTF) protein (SEQ ID NO:1) in which glutamine at position 63 (Q63) is substituted by another amino acid.
- 4. The protein of claim 3, wherein Q63 is substituted by alanine, asparagine, histidine, glutamic acid, or lysine.
- 5. The protein of claim 3, further modified by a substitution of cysteine at position 17 (C17).
- 6. The protein of claim 3 further modified to reduce immunogenicity.
- 7. The protein of claim 6, wherein the modification is a truncation of the C-terminus.
- 8. The protein of claim 7, wherein the truncation is 13 or 15 amino acids.
- 9. The protein of claim 6, further modified by a substitution of tryptophan at position 64 (W64).
- 10. The protein of claim 5, further modified by a substitution of tryptophan at position 64 (W64).
- 11. The protein of claim 5, wherein the substitution at C17 is serine or alanine.
- 12. The protein of claim 5, further modified to reduce immunogenicity.
- 13. The protein of claim 12, wherein the modification is a truncation of the C-terminus.
- 14. The protein of claim 13, wherein the truncation if 13 or 15 amino acids.
- 15. The protein of claim 12, further modified by a substitution of tryptophan at position 64 (W64).
- 16. A human ciliary neurotrophic factor (CNTF) protein (SEQ ID NO:1) which is modified by a substitution of glutamine at position 63 (Q63), and a substitution of tryptophan at position 64 (W64).
- 17. The protein of claim 16, where in the substitution of Q63 is arginine, alanine, asparagine, histidine, glutamic acid, or lysine.
- 18. The protein of claim 16, where in the substitution of W64 is alanine, phenylalanine, histidine, or glutamine.
- 19. The protein of claim 16, where in the substitution of Q63 is arginine and the substitution of W64 is alanine.
- 20. A human ciliary neurotrophic factor (CNTF) protein (SEQ ID NO:1) which is modified by a substitution of glutamine at position 63 (Q63) with alanine, asparagine, histidine, glutamic acid, or lysine, and a substitution of tryptophan at position 64 (W64) with phenylalanine, histidine, or glutamine.
- 21. The protein of claim 20, further modified to reduce immunogenicity.
- 22. The protein of claim 21, wherein the modification is a truncation of the C-terminus.
- 23. The protein of claim 22 wherein the truncation is 13 or 15 amino acids.
STATEMENT OF RELATED APPLICATIONS
[0001] This application is a continuation of U.S. Ser. No. 10/226,759 filed 23 Aug. 2002, which is a divisional of U.S. Ser. No. 09/197,349 filed 20 Nov. 1998, now U.S. Pat. No. 6,440,702, which is a continuation of U.S. Ser. No. 08/308,736 filed 19 Sep. 1994, now U.S. Pat. No. 5,846,935, which is a continuation in part of U.S. Ser. No. 07/959,284 filed 9 Oct. 1992, now U.S. Pat. No. 5,349,056, which applicatins are herein specifically incorporated by reference in their entirety.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09197349 |
Nov 1998 |
US |
Child |
10226759 |
Aug 2002 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
10226759 |
Aug 2002 |
US |
Child |
10383916 |
Mar 2003 |
US |
Parent |
08308736 |
Sep 1994 |
US |
Child |
09197349 |
Nov 1998 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
07959284 |
Oct 1992 |
US |
Child |
08308736 |
Sep 1994 |
US |