Patent Application
20220233499
The present application claims priority from Indian Patent Application No. 202141003895 filed on Jan. 28, 2021, the contents of which are hereby incorporated by reference herein in their entirety.
The present invention pertains to the field of advanced materials. More particularly, the invention relates to a modified PEG-400 (mPEG-AA complex) having antibacterial, antifungal and antiviral activity.
Polyethylene glycol (PEG) is used for various biomedical applications such as drug delivery, due to the high biocompatibility and enhanced circulation time they offer. There is no existing polymer technology which acts as both antimicrobial and antifungal while being biocompatible. Since the inhibition of one commodity/population leads to the growth of others, it is necessary to have a material which can act as both antimicrobial and antiviral material.
Polyethylene glycol (PEG), a polymer made out of ethylene oxide monomers is having various applications in chemical to biomedical industries. The structure of PEG is commonly expressed as H—(O—CH2—CH2)n—OH. PEG is one of the most used polymers used for biomedical applications as drug carriers, for coating various proteins or molecules (PEGylation). The modifications on physical and chemical properties of PEG are usually performed depending on the application.
There is a continuous effort in development of new materials which can be used as a coating agent and has antimicrobial and antiviral activities. Thus, the present invention represents an advancement in the development of new material to solve a long-standing problem of providing materials which can be used as biocompatible coating agents having antimicrobial and antiviral properties.
The primary objective of this invention is to develop a modified PEG 400 having antimicrobial and antiviral activity.
The present invention provides a modified PEG-400-ascorbic acid (mPEG-AA complex) comprising the reaction product of fluorescent polyethylene glycol 400 (FLPEG 400) and ascorbic acid. The invention also provides a process for the preparation of the mPEG-AA complex comprising the steps of: (a) heating PEG 400 at a temperature of about 80-90° C. for about 0.5-1 h to get FLPEG-400; (b) adding ascorbic acid to the FLPEG 400; and (c) heating the mixture of FLPEG 400 and ascorbic acid to obtain the mPEG-AA complex. The mPEG-AA complex exhibits both antimicrobial and antiviral activities.
As those in the art will appreciate, the following detailed description describes certain preferred embodiments of the invention in detail and is thus only representative and does not depict the actual scope of the invention. Before describing the present invention in detail, it is understood that the invention is not limited to the particular aspects and embodiments described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention defined by the appended claims.
The present invention discloses a modified PEG-400 (mPEG-AA complex) having antibacterial, antifungal and antiviral activity. Further, the invention contemplates a multidimensional approach in the development of highly efficacious and cost-effective modified PEG-400.
The present invention is characterized by the following advantages:
Before the compositions and methods of the present disclosure are described in greater detail, it is to be understood that the invention is not limited to particular embodiments and may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting since the scope of the formulations and processes will be limited only by the appended claims.
Embodiments described herein can be understood more readily by reference to the following detailed description, examples, and drawings. Elements, apparatus, and methods described herein are merely illustrative of the principles of the present invention and are not limited to the specific embodiments presented in the detailed description, examples, and drawings. Numerous modifications and adaptations will be readily apparent to those of skill in the art without departing from the spirit and scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the methods belong. Although any nanoformulations, compositions or methods similar or equivalent to those described herein can also be used in the practice or testing of the embodiments of the present invention, representative illustrative methods and compositions are now described.
Where a range of values is provided, it is understood that each intervening value between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within by the methods and compositions. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within by the methods and compositions, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the methods and compositions.
It is appreciated that certain features of the methods, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the methods and compositions, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable sub-combination. It is noted that, as used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as “solely,” “only” and the like in connection with the recitation of claim elements or use of a “negative” limitation.
As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other embodiments without departing from the scope or spirit of the present methods. Any recited method can be carried out in the order of events recited or in any other order that is logically possible.
In one embodiment, the disclosure provides a modified fluorescent polyethylene glycol 400 (mPEG-AA complex) comprising the reaction product of fluorescent polyethylene glycol 400 (FLPEG 400) and ascorbic acid.
In another embodiment, the mPEG-AA complex exhibits a red shifted fluorescence peak wavelength compared with the peak wavelength of fluorescence emitted by the FLPEG 400 and/or polyethylene glycol 400 (PEG 400).
In another embodiment, fluorescent polyethylene glycol 400 (FLPEG 400) is obtained by heating the PEG 400 at a temperature of about 80-90° C. for about 0.5-1 hr.
In yet another embodiment, ascorbic acid as used for the development of mPEG-AA complex is present as in solid form.
In another embodiment, the PEG 400 is heated at a temperature of about 85-90° C. for about 35-45 minutes for the development of FLPEG-400.
In another embodiment, the concentration of ascorbic acid is at least about 5 mg per mL of FLPEG-400.
In another embodiment, the concentration of ascorbic acid is in a range from 17-50 mg per mL of FLPEG-400.
In another embodiment, the invention provides a method for obtaining a modified polyethylene glycol 400 (mPEG-AA complex) by the steps of:
In another embodiment, in step (a) of the method, PEG 400 is heated at a temperature of about 85-90° C. for about 35-45 minutes.
In another embodiment, in step (b) of the method, the ascorbic acid is added in powder form, wherein the concentration of ascorbic acid to FLPEG is in a range from 5 mg per ml of FLPEG-400 to 50 mg per ml of FLPEG-400.
In another embodiment, ascorbic acid is present at a concentration of at least 5 mg per ml of FLPEG-400.
In another embodiment, in step (c) of the method, the mixture is heated at a temperature of 85-90° C. for about 2-20 minutes.
In another embodiment, the invention provides a composition comprising an effective amount of mPEG-AA complex.
The formulation or composition comprising an effective amount of mPEG-AA complex may further comprise one or more active pharmaceutical ingredient, pharmaceutically acceptable carriers or excipient. The carriers include but are not limited to polymers, sterile aqueous media, solid diluents or fillers, excipients, and various non-toxic organic solvents.
The formulation or composition as disclosed herein can be used as a medicament or as a component in a pharmaceutical composition. Pharmaceutical compositions include tablets, capsules, pills, powders, granules, aqueous and non-aqueous oral solutions and suspensions, sprays, suppositories, gels, pastes, ointments, jellies, lotions, injectable solutions, elixirs, syrups, and parenteral solutions packaged in containers adapted for subdivision into individual doses.
Parenteral formulations include pharmaceutically acceptable aqueous or non-aqueous solutions, dispersion, emulsions, suspensions, and sterile powders for the preparation thereof. Non-limiting examples of carriers include water, ethanol, polyols (such as propylene glycol, polyethylene glycol), vegetable oils, and injectable organic esters such as ethyl oleate. Fluidity can be maintained by the use of a coating such as lecithin, a surfactant, or maintaining appropriate particle size. Exemplary parenteral administration forms include solutions or suspensions of the compounds of the invention in sterile aqueous solutions, for example, aqueous propylene glycol or dextrose solutions. Such dosage forms can be suitably buffered, if desired.
An effective amount refers to that amount which has the effect of reducing or inhibiting (that is, slowing to some extent, preferably stopping) one or more signs of a medical symptom.
In certain embodiments, composition or formulation containing mPEG-AA complex may be administered in one or more dosage forms.
Those skilled in the art will be able to determine, according to known methods, the appropriate amount, dose or dosage of the composition for administration to a subject taking into account factors such as age, weight, general health, the compositions administered, the route of administration, the nature and advancement of malignancy requiring treatment, and the presence of other medications.
The composition may be administered together or independently of one another by any route known to a person skilled in the art, such as by oral, intravenous, topical, intraperitoneal or nasal route.
In certain embodiments, the composition is administered at a pre-determined daily dosage. This dosage regimen may be adjusted to provide the optimal therapeutic response. For example, the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation.
The practice of the method of this invention may be accomplished through various administration or dosing regimens. The formulations of the present invention can be administered intermittently, concurrently or sequentially with other prescribed pharmaceutical compositions.
Repetition of the administration or dosing regimens may be conducted as necessary to achieve levels of treatment.
In another embodiment, the mPEG-AA complex may be used as a coating agent in face masks, N95 respirators, elastomeric respirators, powered air-purifying respirators (PAPRs), controlled air-purifying respirators (CAPRs), face shields, personal protection kits and/or other safety accessories and protective packaging covers.
The following examples particularly describe the manner in which the invention is to be performed. These examples are not intended to be inclusive of all aspects of the subject matter disclosed herein, but rather to illustrate representative methods and results. These examples are not intended to exclude equivalents and variations of the present invention which are apparent to one skilled in the art.
For preparing the modified fluorescent PEG with molecular weight 400 (PEG-400, manufactured by Sigma Aldrich, U.S.A) was heated at a temperature of about 85-90° C. for a period of 40 minutes. This reaction led to the synthesis of FLPEG 400 (PEG-400 with enhanced green fluorescence).
Thereafter, ascorbic acid at a concentration of 5 mg/ml of FLPEG was added to the reaction mixture and continuously stirred at a temperature in the range of 85-90° C. for about 5-7 minutes to obtain modified PEG-400 (mPEG-AA complex).
The mPEG-AA complex exhibits a red shift upon addition of ascorbic acid into the pre-heated samples.
The modified PEG-400 (mPEG-AA complex) as obtained in Example 1 was characterized under white light and UV illumination as depicted in
The theoretical study on both ascorbic acid and monoethylene glycol/hexaethylene glycol shows that mPEG-AA complex exist as a single entity through the hydrogen bonding interactions (
PEG 400 was modified with Albumin gold nanoclusters (A-GNCs). Albumin gold nanoclusters (A-GNCs) are inherently red fluorescent. The inventors evaluated the change in the fluorescence with the addition of green fluorescent mPEG-AA complex.
The fluorescence of mixture of A-GNCs and mPEG-AA complex in different ratios and their respective spectra are depicted in
The TEM imaging (
The antibacterial effects of mPEG-AA complex were studied. mPEG-AA complex, modified with ascorbic acid concentration of 5 mg per ml of FLPEG-400 was investigated for antibacterial effect using Escherichia coli and Staphylococcus aureus.
The bacterial culture of Escherichia coli and Staphylococcus aureus was grown till the optical density (O.D.) reached 1.
Thereafter, 200 μl of culture was spread on the LB agar plates separately for each bacterial culture.
A commercially available face-mask was cut into rectangular shape and mPEG-AA complex was coated on the surface of a piece of mask.
mPEG-AA complex coated mask was placed on the bacteria containing agar plate keeping PEG 400, FLPEG coated and uncoated masks as experimental controls as depicted in
Commercially available face-mask material was unable to inhibit the bacterial growth, whereas mPEG-AA complex coated mask was able to inhibit bacterial growth on the agar plate.
Though antibacterial property was visible from a concentration of 50 μg/ml, the mPEG-AA complex was able to inhibit the growth of bacteria at a concentration, starting from 500 μg/ml.
Thus, mPEG-AA complex containing about 5 mg per ml of FLPEG-400 of ascorbic acid was able to inhibit bacterial growth.
The antifungal effects of mPEG-AA complex were studied. Candida albicans was cultured in Sabroud dextrose broth and incubated at 37° C.
The cells were then sub-inoculated in sterile RPMI 1640 media and grown to an optical density (O.D.) of 0.2. The turbidity of the inoculum was adjusted to 0.5 McFarland and diluted in RPMI, corresponding to around 3×105 C.F.U./ml.
The culture was subsequently used for the experiment. 200 μl of culture (triplicates) were plated in the 24-well plate, PEG 400, FLPEG 400 and mPEG-AA complex was added with varying concentrations (50 μg/ml to 300 μg/ml) then incubate the plate at 37° C. for 48 hrs, and viability was evaluated by MTT assay. IC50 value for FLPEG 400 was determined to be 200 μg/ml.
The mPEG-AA complex was found to be a superior antifungal material as compared to FLPEG 400 and PEG 400. The survival of the fungal growth decreases below 50% only when it was treated with 250 μg/ml of FLPEG 400, while it was achieved at a concentration of 100 μg/ml and onwards when treated with mPEG-AA complex.
A spot assay was performed to understand the colony-forming units of Candida albicans. Briefly, 1 mL of five-fold serial dilutions (10, 10−1, 10−2, 10−3 and 10−4) of Candida albicans inoculum from PEG 400, FLPEG 400 and mPEG-AA complex were treated in a 24-micro well plate and was spotted onto SDA agar plates with proper controls. The growth difference was observed after incubation at 37° C. for 48 hrs.
mPEG-AA complex at a concentration of 200 μg/ml, was observed to possess an antifungal property. The spot assay as well shows the antifungal properties of mPEG-AA complex.
The antifungal effects of mPEG-AA complex were studied. Varying concentrations of PEG 400, FLPEG 400 and mPEG-AA complex (50 mg and 100 mg) were incubated with bacteriophage lambda viral load of 1*105 pfu/ml in SM buffer.
Post 24 hours of incubation carried out at 37° C., dose-response inactivation was observed in quantitative way of analysis.
Plaque formation assay was performed for the quantitative assessment with which an IC50 value of 200 mg/ml of mPEG-AA complex was observed.
Qualitative analysis with drop-cast method also validates the same, but rather much more pronounced effect was exhibited when incubated with mPEG-AA complex in comparison to the FLPEG 400 of the same concentration.
mPEG-AA complex was able to act as an antiviral material with the lowest concentration of 50 mg/ml. It was obtained that a concentration of 100 mg/ml was able to completely inhibit the viral growth in the medium.
It was further determined that in the mPEG-AA complex, an ascorbic acid concentration of ˜10 mM inhibits total viral growth.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202141003895 | Jan 2021 | IN | national |
