MODIFIED SITE-SPECIFIC ENDONUCLEASE FOR GENOME RESEARCH

Information

  • Research Project
  • 3500518
  • ApplicationId
    3500518
  • Core Project Number
    R43HG000700
  • Full Project Number
    1R43HG000700-01A1
  • Serial Number
    700
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/30/1992 - 31 years ago
  • Project End Date
    6/30/1993 - 30 years ago
  • Program Officer Name
  • Budget Start Date
    9/30/1992 - 31 years ago
  • Budget End Date
    6/30/1993 - 30 years ago
  • Fiscal Year
    1992
  • Support Year
    1
  • Suffix
    A1
  • Award Notice Date
    9/30/1992 - 31 years ago
Organizations

MODIFIED SITE-SPECIFIC ENDONUCLEASE FOR GENOME RESEARCH

With the rapid growth of human genome research, there is a growing need for advanced mapping and sequencing methods. In current methodology, there is no single step process for generating specific DNA sequencing methods. In current methodology, there is no single step process for generating specific DNA fragments in the megabase size range. Promega proposes to undertake random mutagenesis coupled with a powerful, novel genetic selection to isolate forms of an intron-encoded site-specific endonuclease--I-Ppo--with potential for use in genome analysis. These goal of these studies would be to develop 1) a family of enzymes with a range of cutting frequencies in the 2-1-- megabase size range, 2) advanced methods for analyzing novel DNA-protein interaction, and 3) advanced genetic selection methodology useful for detailed analysis of the determinants for recognition and catalysis by other site-specific endonucleases. To demonstrate the feasibility of achieving these results, Phase I research will focus on two specific aims: 1) Systematically examine the toleration for base substitutions within the canonical 15 base pair I-Ppo recognition sequence. 2) Demonstrate the feasibility of a genetic selection for mutant enzymes with altered specificity. Subsequent to Phase I and Phase II studies, Promega would commercialize the rare-cutting enzymes, and seek strategic alliances to incorporate the enzymes into automated DNA mapping and analysis applications.

IC Name
NATIONAL HUMAN GENOME RESEARCH INSTITUTE
  • Activity
    R43
  • Administering IC
    HG
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    172
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    PROMEGA CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    MADISON
  • Organization State
    WI
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    537115399
  • Organization District
    UNITED STATES