Claims
- 1. An injection composition comprising:
- (a) a pharmacologically effective amount of a modified tPA selected from the group consisting of:
- (i) a mutant tPA prepared by eliminating regions F and G of natural tPA and replacing Ser at an amino acid position No. 119 thereof with Met,
- (ii) a mutant tPA prepared by replacing K.sub.1 region of natural tPA with K.sub.1 region of plasminogen, and
- (iii) a mutant tPA prepared by replacing Cys at an amino acid position No. 84 of natural tPA with Ser;
- (b) at least 47.5 mM of a basic or acidic amino acid, a salt thereof, or mixture thereof; and
- (c) a pharmacologically acceptable carrier.
- 2. The injection composition as claimed in claim 1, wherein said modified tPA is the mutant tPA prepared by replacing Cys at an amino acid position No. 84 of natural tPA with Ser.
- 3. The injection composition as claimed in claim 1, wherein said basic amino acid is selected from the group consisting of arginine, histidine, lysine, ornithine and a salt thereof.
- 4. The injection composition as claimed in claim 1, wherein said acidic amino acid is selected from the group consisting of glutamic acid, aspartic acid and a salt thereof.
- 5. The injection composition as claimed in claim 1, in which the modified tPA is a mutant tPA prepared by replacing Cys at an amino acid position No. 84 of natural tPA with Ser and the amino acid is a mixture of arginine or a salt thereof and aspartic acid or a salt thereof.
- 6. The injection composition as claimed in claim 1, wherein said modified tPA is the mutant tPA prepared by replacing Cys at an amino acid position No. 84 of natural tPA with Ser and said amino acid salt is arginine aspartate.
- 7. The injection composition according to claim 6, wherein the concentration of said amino acid is at least 142.5 mM.
- 8. The injection composition according to claim 1, wherein said amino acid is selected from the group consisting of L-arginine, L-histidine, L-aspartate, L-glutamate, L-glycine, salts thereof, and mixtures thereof.
- 9. The injection composition according to claim 8, wherein said amino acid is selected from the group consisting of L-arginine hydrochloride, L-histidine hydrochloride, sodium L-aspartate, sodium L-glutamate, and L-arginine-L-aspartate.
- 10. The injection composition according to claim 8, wherein the concentration of said amino acid is at least 142.5 mM.
- 11. The injection composition according to claim 1, wherein the concentration of said amino acid is at least 142.5 mM.
- 12. The injection composition according to claim 1, wherein the concentration of said amino acid is 95 mM.
- 13. The injection composition according to claim 1, wherein the pH of the composition is in the range of 5 to 7.
- 14. An injection composition comprising:
- (a) a pharmacologically effective amount of a mutant tPA prepared by replacing Cys at an amino acid position No. 84 of natural tPA with Ser;
- (b) at least 47.5 mM of L-arginine-L-aspartate; and
- (c) a pharmacologically acceptable carrier.
Priority Claims (1)
Number |
Date |
Country |
Kind |
62-199381 |
Aug 1987 |
JPX |
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Parent Case Info
This is a continuation of Ser. No. 07/576,188, filed Aug. 27, 1990, now abandoned, which is a continuation of application Ser. No. 07/230,148, filed Aug. 9, 1988, now abandoned.
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Continuations (2)
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Number |
Date |
Country |
Parent |
576188 |
Aug 1990 |
|
Parent |
230148 |
Aug 1988 |
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