Claims
- 1. Essentially pure human H2-relaxin, which is free of other human proteins.
- 2. An essentially pure polypeptide having relaxin activity, wherein said polypeptide comprises:
- (i) a human H2-relaxin A chain selected from the group consisting of A(1-24) to A(5-24), wherein amino acids 1-24 have the following sequence: ##STR1## and (ii) a human H2-relaxin B chain selected from the group consisting of B(-1-32) to B(4-23), wherein amino acids -1-32 have the following sequence: ##STR2##
- 3. The essentially pure polypeptide according to claim 2, wherein said polypeptide comprises:
- (i) a human H2-relaxin A chain selected from the group consisting of A(1-24) to A(3-24); and
- (ii) a human H2-relaxin B chain selected from the group consisting of B(-1-32) to B(-1-24).
- 4. The essentially pure polypeptide as claimed in claim 2, wherein the B chain has been modified by one or more procedures selected from the group consisting of
- (a) formylation of the Trp residue at B(2); and
- (b) replacement of the Met residue at B(24) with a member selected from the group consisting of norleucine, valine, alanine, glycine, serine and homoserine.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PF7247 |
Dec 1982 |
AUX |
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Parent Case Info
This is a Division of application Ser. No. 560,790, filed 12/13/83, now U.S. Pat. No. 4,758,516.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
4267101 |
Bagazzi |
May 1981 |
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4624804 |
Voelter et al. |
Nov 1986 |
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4656249 |
Tregear et al. |
Apr 1987 |
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Non-Patent Literature Citations (4)
Entry |
Hudson et al. "Molecular Cloning and Characterization of cDNA Sequences Coding for Rat Relaxin" Nature vol. 291, pp. 127-131, 14 May 1981. |
Fields, P. A. "Purification and Immunohistochemical Localization of Relaxin . . . " J. of Clinical Endocrinology & Metabolism 52(1)79-85(1981). |
Haley, J. et al. "Porcine Relaxin . . . " DNA vol. 1(2) Mar. 1982 pp. 155-162. |
Chem. Abstracts 97:727496 (8/30/82). |
Divisions (1)
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Number |
Date |
Country |
Parent |
560790 |
Dec 1983 |
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