Claims
- 1. An E. coli host cell transformed with the plasmid pBSEl of ATCC 67500, which encodes the functional neutral protease isolated from Bacillus stearothermophilus NRRL-B-3880, wherein said E. coli expresses an active, functional neutral protease enzyme which exhibits activity in a milk clearing assay.
- 2. The E. coli of claim 1 which is E. coli ATCC 67500.
Parent Case Info
This is a continuation-in-part of U.S. Ser. No. 103,983, now abandoned entitled "Molecular Cloning and Expression of Protease Genes" (A. H. Deutch and V. A. David), filed Oct. 1, 1987.
This is a continuation of application Ser. No. 123,038, filed Nov. 19, 1987.
Foreign Referenced Citations (1)
Number |
Date |
Country |
58-162291 |
Jan 1984 |
JPX |
Non-Patent Literature Citations (2)
Entry |
Takayi et al. J. Bact vol. 163, pp. 824-831. |
Maniatis et al. Molecular Cloning, A laborator Manual Cold Harbor Laboratory CHS, NY (1982) pp. 404-411. |
Continuations (1)
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Number |
Date |
Country |
Parent |
123038 |
Nov 1987 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
103983 |
Oct 1987 |
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