MOLECULAR CLONING OF CDNA ENCODING OXIDIZED LDL RECEPTOR

Information

  • Research Project
  • 3502059
  • ApplicationId
    3502059
  • Core Project Number
    R43HL047220
  • Full Project Number
    1R43HL047220-01
  • Serial Number
    47220
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/30/1991 - 33 years ago
  • Project End Date
    3/29/1992 - 33 years ago
  • Program Officer Name
  • Budget Start Date
    9/30/1991 - 33 years ago
  • Budget End Date
    3/29/1992 - 33 years ago
  • Fiscal Year
    1991
  • Support Year
    1
  • Suffix
  • Award Notice Date
    9/27/1991 - 33 years ago

MOLECULAR CLONING OF CDNA ENCODING OXIDIZED LDL RECEPTOR

DESCRIPTION (Adapted from applicant's abstract): The long term objective of this research is to develop drugs to prevent progression/formation of atherosclerotic lesions in humans. Lipid deposition in the arterial wall has been shown in animal models to involve the initial accumulation of lipid by macrophages. Evidence to date suggests that this lipid is taken up in the form of oxidized low-density lipoprotein (LDL), by two macrophage receptor, the acetyl-LDL receptor, and a distinct oxidized-LDL receptor. The intent is to identify compounds which will block lipid uptake by the oxidized-LDL receptor. During Phase I, it is proposed to clone the cDNA encoding the oxidized-LDL receptor from macrophages. A mouse macrophage cDNA library will be expressed transiently in mammalian cells, and the clone for the receptor will be isolated by screening for the cell surface expression of receptors which bind oxidized-LDL. In order not to clone the acetyl-LDL receptor, binding studies will be carried out under conditions in which the acetyl-LDL receptor is blocked. During Phase II, the oxidized-LDL receptor will be stably expressed in a cell line, and this line will be used to screen libraries of compounds for blocking activity.

IC Name
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
  • Activity
    R43
  • Administering IC
    HL
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    837
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    CALIFORNIA BIOTECHNOLOGY, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    MOUNTAIN VIEW
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    94043
  • Organization District
    UNITED STATES