Patent Grant
9205156
The present invention generally relates to labeled ammonium salts and uses thereof as myocardial perfusion agents in molecular imaging.
Myocardial perfusion imaging (MPI) is the most common tool for non-invasively evaluating ischemia in patients with suspected coronary artery disease (CAD). This technique contributes substantially to the risk-stratification of CAD patients, in terms of their likelihood to encounter coronary events and cardiac death. Therefore, MPI provides valuable information which assists clinical decision-making with regard to medical treatment and intervention. There is a growing and consistent evidence that gated MPI provides additional clinically useful data towards patient risk stratification, by enabling the assessment and comparison of left ventricular function during both post-stress and rest conditions.
Current data suggest that positron emission tomography (PET) MPI is a powerful modality for diagnosing obstructive CAD, and appears to provide better diagnosis than single photon emission computed tomography (SPECT); this is primarily related to PET technological advantages, such as higher count sensitivity, better spatial resolution and more accurate attenuation correction. The ability to quantify myocardial perfusion in absolute values is an added advantage of PET over SPECT, particularly in multivessel CAD.
Despite the known advantages of PET, SPECT tracers and [99mTc]-MIBI, in particular, are more prevalent in clinical practice. This is primarily due to the two following reasons: (i) the use of SPECT radiopharmaceuticals is logistically easier, and tracer dose price is usually lower than the respective cyclotron-produced PET pharmaceuticals, and (ii) existing PET MPI probes have short physical half lives, which require in-house production, and more importantly, have sub-optimal pharmacokinetic profiles, which most likely withhold the shift of nuclear cardiology from SPECT to the superior PET modality.
Nevertheless, in light of the potential contribution of PET to the field of MPI, increasing efforts were invested into the development of new PET MPI probes.
In the last decade, a wealth of effort has been invested into the development of optimal PET myocardial perfusion agents. Several fluorine-18 labeled myocardial flow tracers were reported in the literature. [18F]RP1004 [1], [18F]FBnTP [2, 3] and [18F]FDHR [3] base their cardiac accumulation on interactions with mitochondria which are plentiful in the heart muscle. The longer half-life of fluorine-18 enables extensive radiochemical transformations, extended PET scan times and commercial distribution. A wait of a period of four to five half-lives is ideally required between equal doses administered at rest and stress tests to allow for radioactivity from the first injection to decay to a point where it does not interfere significantly with the later scan. Fluorine-18 based tracers may prevent performance of both scans during a single day or at least require several hours stay interval while using an initial low dose followed by a high dose later, similarly to a “one-day protocol” used with 99mTc-labled SPECT flow tracers.
Several publications discuss the utilization of radiolabled tracers in molecular imaging techniques such as PET.
A main prerequisite for a positron emission tomography (PET) tracer to be considered as an optional candidate for myocardial perfusion imaging (MPI) is an elevated myocardial tracer concentration with simultaneous decrease in adjacent compartments, mainly the blood pool and lungs. Rapid and elevated myocardial concentration may be indicative of high tracer extraction during tracer passage through the coronary microcirculation. Rapid washout of tracer from background tissues creates target to non-target image contrast and thus improves the MPI diagnostic process. In addition, a tracer with prolonged stable myocardial activity levels confers an additional advantage, as this quality allows for the performance of the stress test and tracer injection outside the PET room. This is particularly important when a treadmill stress test is clinically indicated or is preferable. Renal excretion of the tracer is also favored over hepatic pathways to minimize the tracer's biological half-life, thus reducing radiation dose to the patient. Labeling the tracer with carbon-11 with its short physical half-life (20.4 min), while restricting its usage to the cyclotron unit's vicinity, is associated, by itself, with favorable radiation dosimetry, and also allows the performance of a rest study an hour and a half after the stress injection without significant residual crosstalk.
The present invention is based on the observed biodistribution in mice and rats and both small- and large-animal imaging using a microPET/CT and a clinical PET/CT, respectively, which supports the superiority of compounds of the invention over those previously known. The results presented herein clearly suggest that organic ammonium salts of the genera disclosed herein offer a great spectrum of advantages and open up the path for new PET applications for MPI. Thus, the invention generally provides novel compounds, compositions comprising same for, e.g., diagnostic applications, pharmaceutical compositions, radiopharmaceuticals and uses thereof in a variety of applications.
In one aspect of the present invention there is provided a radiolabeled compound of Formula (I):
wherein
each of R1, R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH, each of the aforementioned may optionally be substituted as disclosed herein;
at least one of R1, R2, R3 and R4 is or comprises at least one radiolabeled atom or substituent (which comprises one or more radiolabled atom);
each of n and m, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion (anion).
Each of the substituents recited above for R1, R2, R3 and R4, independently of each other, is optionally substituted by one or more substituent selected from C1-C6alkyl, C6-C18aryl, halide (Cl, Br, I, F), nitro, amine, hydroxyl, ether, and others.
In some embodiments in a compound of Formula (I), each of the alkyl group in said C1-C10alkyl, C3-C10cycloalkyl, C1-C10aralkyl, and polyethyleneglycol (PEG), independently of the other, and irrespective of their position on the ammonium center (namely whether designated as R1, R2, R3 and/or R4) may be substituted as stated above. In some embodiments, the alkyl group is substituted by at least one halide. In other embodiments, the alkyl group is substituted by one or more F atoms.
In some embodiments, where the alkyl group is C1-C10alkyl, or C1-C10aralkyl, the alkyl group has 1, 2, or 3 carbon atoms, one of which carbon atoms being substituted by one or more F atoms (in some embodiments, 1 carbon atom). In other embodiments, the alkyl group has 1 or 2 carbon atoms, one of which carbon atoms being substituted by one or more F atoms (in some embodiments, 1 carbon atom). For example, where the alkyl group is C1-C10alkyl having 2 carbon atoms, the alkyl group is an ethyl group, one of which carbon atoms being substituted by an F atom. Where the alkyl group is polyethylenglycol (PEG), one of the ethylene carbons may be substituted by an F atom.
In some embodiments, at least one of said R1, R2, R3 and R4, independently of the other, is an alkyl group having 1, 2, 3, or 4 carbon atoms, one of which being substituted by one or more F atoms.
In some embodiments, at least one of said R1, R2, R3 and R4, independently of the other, is an alkyl group having 1 or 2 carbon atoms, one of which being substituted by one or more F atoms.
In some embodiments, at least one of said R1, R2, R3 and R4, independently of the other, is an alkyl group having 1 carbon atom (in some embodiments, 2 carbon atoms; in another embodiment 3 carbon atoms), one of which being substituted by one or more F atoms.
In some embodiments, any of the above F atoms substituting an alkyl group is 18F.
In some embodiments, in a compound of Formula (I), at least one of R1, R2 and R3 is a C1-C10alkyl. In other embodiments, at least two of R1, R2 and R3 is a C1-C10alkyl. In further embodiments, each of R1, R2 and R3 is a C1-C8alkyl. In each of said embodiments, each of the substituents, independently, is optionally substituted.
In some embodiments, R4 is a radiolabeled C1-C8alkyl.
In other embodiments, at least one of R1, R2 and R3 is a C1-C10alkyl and R4 is a C6-C18aryl.
In yet additional embodiments, two of R1, R2 and R3 are each, independently, a C1-C10alkyl and the other of R1, R2 and R3 is a C6-C18aryl and R4 is a C6-C18aryl. In such embodiments, for example, each of R1 and R2 is a C1-C10alkyl and each of R3 and R4, independently, is a C6-C18aryl.
In some embodiments, at least one of R1, R2 and R3 is a C6-C18aryl, being optionally substituted. In some embodiments, at least one of R1, R2 and R3 is a C6-C18aryl, being optionally substituted, and R4 is a C1-C10alkyl.
In some embodiments, each of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C10alkyl.
In some embodiments, at least one of said R1, R2, R3 and R4 is a C1-C10alkyl different from a methyl, and at least one of said R1, R2, R3 and R4 is a methyl group. The C1-C10alkyl may or may not be substituted.
In some embodiments, each of R1, R2, R3 and R4 is a methyl group, at least one which being radiolabeled. An exemplary compound is Compound [11C]-1.
In some embodiments, two of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C10alkyl and the other two of R1, R2, R3 and R4, independently of the other, is an optionally substituted C6-C18aryl.
In some embodiments, three of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C6alkyl and the other one of R1, R2, R3 and R4 is an optionally substituted C6-C18aryl.
In other embodiments, in a compound according to Formula (I), R1 is a C6-C18aryl, said aryl being in certain embodiments a substituted or unsubstituted phenyl ring. Thus, the compound is of Formula (II):
wherein
each of R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m, —OH;
at least one of R2, R3 and R4 is or comprises at least radiolabeled atom or substituent;
R5 is one or two (referred to as R51, R52) or three (referred to as R51, R52, R53) or four (referred to as R51, R52, R53, R54) or five substitutions (referred to as R51, R52, R53, R54, R55) on the ring, the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C6alkyl, —N(C1-C6alkyl)2), hydroxyl (—OH), C1-C6alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C6alkoxy (—O—C1-C6alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion (anion).
In some embodiments, in a compound of Formula (II), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl. In some further embodiments, one of R2, R3, and R4 is a C1-C10 alkyl, and another of R2, R3, and R4 is PEG. In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10 alkyl and the third of said one of R2, R3, and R4 is PEG.
In some embodiments, in a compound of Formula (II), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl, one of which being substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In some further embodiments, one of R2, R3, and R4 is a C1-C10alkyl, and another of R2, R3, and R4 is PEG, one or both of C1-C10alkyl and PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10alkyl and the third of said one of R2, R3, and R4 is PEG, wherein one or both of said C1-C10alkyl and/or said PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom).
In some embodiments, in a compound of Formula (II), each of R2, R3 and R4 is a C1-C10alkyl, at least one of said R2, R3 and R4 comprising at least one radiolabeled atom or substituent.
In other embodiments, in a compound of Formula (II), at least one of R51, R52, R53, R54, R55 is —H or a C1-C10alkyl.
In further embodiments, each of R51, R52R53R54R55 is —H, R2, each of R2, R3 and R4, independently of the other is a radiolabeled C1-C10alkyl selected from a methyl, ethyl, propyl, iso-propyl and n-butyl.
In other embodiments, each of R2, R3 and R4, independently of the other is a radiolabeled methyl or an ethyl.
In further embodiments, each of R2, R3 and R4 is a methyl, one of said methyl groups being radiolabeled. An exemplary compound is thus Compound [11C]-2 of the invention.
In other embodiments, R5 is one or more substituents, as detailed hereinabove, each being independently selected from halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), hydroxyl (—OH), C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH.
In some embodiments, R5 is a single substituent at ay position of the phenyl ring (substituting any of the 5 ring hydrogens). In some embodiments, R5 is a single substituent at the ortho position. In some embodiments, R5 is a single substituent at the meta position. In some embodiments, R5 is a single substituent at the para position.
In further embodiments, R5 is a halide (i.e., Cl, Br, I, F), an amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), an hydroxyl (—OH), or a C1-C10alkyl. In some embodiments, R5 is —NH2. In further embodiments, R5 is —N(C1-C10alkyl)2, wherein each of the two C1-C10alkyl groups in —N(C1-C6alkyl)2 is same or different. In some embodiments, the two C1-C10alkyl groups in —N(C1-C10alkyl)2 are the same, being selected from methyl and ethyl. In further embodiments, each of the two groups is a methyl. A non-limiting example of such a compound is Compound [11C]-3.
In other embodiments, R5 is a C1-C10alkyl. In some embodiments, is the C1-C10alkyl is selected from a methyl, ethyl, propyl, iso-propyl and n-butyl. In some embodiments, the C1-C10alkyl is a methyl group. A non-limiting example is Compound [11C]-6.
In still other embodiments, in a compound according to Formula (I), R1 is a C6-C18aryl, said aryl being different from a phenyl. In some embodiments, the C6-C18aryl is a naphthyl and the compound is of Formula (III):
wherein the naphthyl moiety may be bonded to the N atom of the quarternary ammonium group via any of the naphthyl carbon atoms, and wherein
each of R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH;
at least one of R2, R3 and R4 is or comprises at least radiolabeled atom or substituent;
R5 is one or two or three or four or five or six or seven substitutions on the naphthyl ring, the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), hydroxyl (—OH), C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C10aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion (anion).
In some embodiments, in a compound of Formula (III), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl. In some further embodiments, one of R2, R3, and R4 is a C1-C10 alkyl, and another of R2, R3, and R4 is PEG. In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10 alkyl and the third of said one of R2, R3, and R4 is PEG.
In some embodiments, in a compound of Formula (III), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl, one of which being substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In some further embodiments, one of R2, R3, and R4 is a C1-C10alkyl, and another of R2, R3, and R4 is PEG, one or both of C1-C10alkyl and PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10alkyl and the third of said one of R2, R3, and R4 is PEG, wherein one or both of said C1-C10alkyl and/or said PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom).
In some embodiments, the compound of Formula (III) is of the Formula (IV):
wherein each of R2, R3, R4 and R5 is as defined above.
In some embodiments, R5 is —H.
In some embodiments, in a compound of Formula (IV), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl. In some further embodiments, one of R2, R3, and R4 is a C1-C10 alkyl, and another of R2, R3, and R4 is PEG. In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10 alkyl and the third of said one of R2, R3, and R4 is PEG.
In some embodiments, in a compound of Formula (IV), at least one of R2, R3, and R4, independently of the other, is a C1-C10alkyl, one of which being substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In some further embodiments, one of R2, R3, and R4 is a C1-C10alkyl, and another of R2, R3, and R4 is PEG, one or both of C1-C10alkyl and PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In further embodiments, two of said at least one of R2, R3, and R4 is a C1-C10alkyl and the third of said one of R2, R3, and R4 is PEG, wherein one or both of said C1-C10alkyl and/or said PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom).
In further embodiments, in a compound of Formula (IV), each of R2, R3 and R4 is a C1-C10alkyl, at least one of said R2, R3 and R4 comprising at least one radiolabeled atom or substituent.
In further embodiments, each of R2, R3 and R4, independently of the other is a radiolabeled C1-C10alkyl selected from a methyl, ethyl, propyl, iso-propyl and n-butyl.
In other embodiments, each of R2, R3 and R4, independently of the other is a radiolabeled methyl or an ethyl.
In further embodiments, each of R2, R3 and R4 is a methyl, one of said methyl groups being radiolabeled. An exemplary compound is thus Compound [11C]-7 of the invention.
In other embodiments, in a compound according to Formula (I), each of R1 and R2 is a C6-C18aryl, said aryl being in certain embodiments a substituted or unsubstituted phenyl ring. Thus, the compound is of Formula (V):
wherein
each of R3 and R4, independently of the other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH;
at least one of R3 and R4 is or comprises at least radiolabeled atom or substituent;
each of R5 and R6, independently of the other, is one or two or three or four or five substitutions on the respective rings (as detailed hereinabove), the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), hydroxyl (—OH), C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol (PEG), C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion (anion).
As used herein, “an integer between 2 and 10” is 2, 3, 4, 5, 6, 7, 8, 9, or 10. Any of the aforementioned integers may constitute n, m, z or y independently.
In some embodiments, in a compound of Formula (V), each of R3 and R4 is a C1-C10alkyl, at least one of said R3 and R4 comprising at least one radiolabeled atom or substituent.
In other embodiments, in a compound of Formula (V), at least one of the substituting R5, and/or R6, representing a single substituting or multiple substituting groups is —H or a C1-C10alkyl.
In some embodiments, in a compound of Formula (V), at least one of R3, and R4, independently of the other, is a C1-C10alkyl. In some further embodiments, one of R3, and R4 is a C1-C10 alkyl, and the other of R3, and R4 is PEG. In further embodiments, each R3 and R4 is a C1-C10 alkyl.
In some embodiments, in a compound of Formula (V), at least one of R3, and R4, independently of the other, is a C1-C10alkyl, one of which being substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In some further embodiments, one of R3 and R4 is a C1-C10alkyl, and the other of R3 and R4 is PEG, one or both of C1-C10alkyl and PEG is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom). In further embodiments, both R3 and R4 is a C1-C10alkyl, wherein one or both of said C1-C10alkyl is substituted with one or more F atoms (1, 2, or 3 F atoms; in some embodiments-1 F atom).
In further embodiments, each of R5 and/or R6 is —H, each of R3 and R4, independently of the other is a radiolabeled C1-C10 alkyl selected from a methyl, ethyl, propyl, iso-propyl and n-butyl.
In other embodiments, each of R3 and R4, independently of the other is a radiolabeled methyl or ethyl.
In further embodiments, each of R3 and R4 is methyl, one of said methyl groups being radiolabeled. An exemplary compound is thus Compound [11C]-4 of the invention.
In other embodiments, at least one of R3 and R4 is not a methyl group. In some embodiments, at least one of R3 and R4 is an ethyl group and the other of R3 and R4 is a methyl group. One of said of R3 and R4 is optionally substituted. An exemplary compound is Compound [18]-5.
As used herein, the term “—(C1-C10)alkyl” refers to a straight or branched aliphatic chain containing between 1 and 10 carbon atoms, which may or may not be substituted. In some embodiments, the —(C1-C10)alkyl is an alkyl group having 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 carbon atoms. In some embodiments, said —(C1-C10)alkyl is an alkyl group having 1, 2, 3, 4, or 5 carbon atoms. In other embodiments, said —(C1-C10)alkyl is an alkyl having 1, 2, or 3 carbon atoms. In further embodiments, said —(C1-C10)alkyl is an alkyl having 1 or 2 carbon atoms.
Thus, the term “—(C1-C10)alkyl” encompasses the various ranges of carbon atom numbers. The term encompasses and is interchangeable with C1-C2 alkyl, C2-C3alkyl, C3-C4alkyl, C4-C5alkyl, C5-C6alkyl, C6-C7alkyl, C7-C8alkyl, C8-C9alkyl, C9-C10 alkyl, C1-C3alkyl, C2-C4alkyl, C3-C5alkyl, C4-C6alkyl, C5-C7alkyl, C6-C8alkyl, C7-C9alkyl, C8-C10alkyl, C1-C4alkyl, C2-C5alkyl, C3-C6alkyl, C4-C7alkyl, C5-C8alkyl, C6-C9alkyl, C7-C10alkyl, C1-C5alkyl, C2-C6alkyl, C3-C7alkyl, C4-C8alkyl, C5-C9alkyl, C6-C10alkyl, C1-C6alkyl, C2-C7alkyl, C3-C8alkyl, C4-C9alkyl, C5-C10alkyl, C1-C7alkyl, C2-C8alkyl, C3-C9alkyl, C4-C10alkyl, C1-C8alkyl, C2-C9alkyl, C3-C10alkyl, C1-C9alkyl, C2-C10alkyl, C1alkyl, C2alkyl, C3alkyl, C4alkyl, C5alkyl, C6alkyl, C7alkyl, C8alkyl, C9alkyl or C10alkyl.
In some embodiments, wherein, for example, R1 and R2 (or any other groups or group combination) are said to independently of each other be selected from C1-C10alkyl, the alkyl groups need not be the same, in terms of length, nor in terms of substitution. For example, in some cases R1 may be a C1-C10alkyl selected amongst C1-C3alkyls and R2 may a C1-C10alkyl selected amongst C3-C7alkyls.
Non-limiting examples of such alkyl chains include methyl, ethyl, propyl, iso-propyl, iso-butyl, n-butyl, sec-butyl, tert-butyl, pentyl, iso-hexyl, heptyl, octyl, nonyl and decyl.
In some embodiments, the —(C1-C10)alkyl is a methyl.
In other embodiments, the —(C1-C10)alkyl is different from a methyl (namely, may be an alkyl having 2, or 3, or 4, or 5, or 6, or 7, or 8, or 9, or 10 carbon atoms).
Where reference is made to “—(C1-C6)alkyl” the —(C1-C6)alkyl is an alkyl group having 1, 2, 3, 4, 5 or 6 carbon atoms. In some embodiments, said —(C1-C6)alkyl is an alkyl group having 1, 2, 3, 4, or 5 carbon atoms. In other embodiments, said —(C1-C6)alkyl is an alkyl having 1, 2, or 3 carbon atoms. In further embodiments, said —(C1-C6)alkyl is an alkyl having 1 or 2 carbon atoms.
Thus, the term “—(C1-C6)alkyl” encompasses the various ranges of carbon atom numbers. The term encompasses and is interchangeable with C1-C2 alkyl, C2-C3alkyl, C3-C4alkyl, C4-C5alkyl, C5-C6alkyl, C1-C3alkyl, C2-C4alkyl, C3-C5alkyl, C4-C6alkyl, C1-C4alkyl, C2-C1alkyl, C3-C1alkyl, C1-C1alkyl, C2-C1alkyl, C1alkyl, C1alkyl, C1alkyl, C4alkyl, C1alkyl, or C1alkyl.
In some embodiments, any of the alkyl groups disclosed herein in reference to a compound of Formula (I), (II), (III), (IV) or (V) may be substituted with one or more F atoms, as recited hereinabove with reference to a compound of Formula (I).
As used herein, the term “—N((C1-C10)alkyl)2” refers to a secondary amine (wherein one of R7 and R8 is a (C1-C10)alkyl), wherein the two —(C1-C10)alkyl groups may be the same or different. The —(C1-C10)alkyl is as defined above. The N atom may be further protonated or alkylated to a charged state, forming a salt with, e.g., at least one pharmaceutically acceptable counterion.
In some embodiments, in the —N((C1-C10)alkyl)2 group, the two —(C1-C10)alkyl groups form a cyclic structure with the N atom they are bonded to; the cyclic amine having between 3 and 6 atoms in the heteroring structure. In some embodiments, the heteroring comprises, apart from the N atom, one or more additional heteroatoms selected from N, O and S. In further embodiments, the heteroring comprises a single heteroatom (the N atom of the —N((C1-C10)alkyl)2) with the remaining atoms being carbon atoms.
As used herein, “—(C6-C18)aryl” refers, in the context of the present invention, to an aromatic ring system having between 6 and 18 carbon atoms. In some embodiments, the aryl is a substituted or unsubstituted phenyl or a substituted or unsubstituted naphthyl group.
As known in the art, polyethylenglycol (PEG) is an ether of the general formula —(O—CH2—CH2)k—OH. In accordance with the present invention, the PEG moiety may be associated with the N atom of the ammonium molecule via the bond N—O, wherein N is the ammonium N atom and O is the PEG end-chain atom, or via a carbon spacer (linker) such as an alkyl. In some embodiments, the PEG is associated directly with the N atom of the ammonium molecule. In other embodiments, the PEG is associated through a carbon spacer affording, e.g., C1-C6alkyl-(O—CH2—CH2)y—OH, wherein y is as defined above. In the general formula —(O—CH2—CH2)k—OH representing PEG, k is an integer between 2 and 10. K may be 2, 3, 4, 5, 6, 7, 8, 9, or 10.
In reference to groups R5 and R6, substituting on an aryl moiety, it is said that each represents “one or two or three or four or five substitutions on the ring”. In this context, it should be noted that one ring may be substituted by a single substituent (R5) and the other ring may be substituted by more than one substituent (R6), as selected above. When the ring bearing the group R5 and/or the ring bearing the group R6 is substituted by a single substituting group, the single group may be substituted on any one of the ring positions, i.e., ortho, meta, or para. When the ring(s) is substituted by two substituting groups, the two groups may be on neighboring carbon atoms (ortho to each other), or may be separated by one or more carbon atoms. When the ring(s) is substituted by three or more substituting groups, the groups may be substituted on any one of the ring positions at any variation available.
The negatively charged “pharmaceutically acceptable ion” (anion), being X in the general formula (I) is an anion derived from an inorganic acid or from an organic acid. In some embodiments, the pharmaceutically acceptable anion is derived from inorganic acids thus may be selected from sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, nitrate, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, caprylate, isobutyrate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, mandelate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, phthalate, benzenesulfonate, toluenesulfonate, phenylacetate, citrate, lactate, maleate, tartrate, methanesulfonate, and the like. Also contemplated are salts of amino acids such as arginate and the like and gluconate, galacruronate (For further anions suitable in compounds of the invention, see, for example, Berge S. M., et al., “Pharmaceutical Salts,” J. of Pharmaceutical Science, 66:1-19 (1977)).
The invention also provides a compound selected from:
In a further aspect of the present invention there is provided a radiopharmaceutical being or comprising a compound of Formula (I) or any of Formula (II), (III), (IV) and (V), as defined herein.
As used herein, the term “radiopharmaceutical” (or ‘radiopharmaceutical formulation’) refers to a formulation comprising at least one compound of general Formulae disclosed herein, as defined herein, namely at least one radiolabeled tracer, in a form suitable for administration to a mammal, such as a human. The tracer may be radiolabeled by any radioisotope known. In some embodiments, the radioisotope used for labeling the tracer is selected from 18F, 15O, 13N and 11C.
In some embodiments, the radioisotope is 11C.
In some embodiments, the radioisotope is 18F.
In some embodiments, the radioisotope is 13N.
In some embodiments, the radioisotope is not 18F.
Typically, the radioisotope is 11C or a radioisotope bonded directly to at least one of the alkyl groups in the formulae of the invention. The radioisotope is typically not bonded to an aryl (e.g., phenyl, naphthyl) group.
In some embodiments, where the radioisotope is 18F it is not bonded to an aryl (e.g., phenyl, naphthyl) group but rather to an alkyl group.
The radiopharmaceutical may be in the form of an aqueous solution. The radiopharmaceutical formulation may optionally further comprise additional components such as a pH-adjusting agent, pharmaceutically acceptable stabilizers or antioxidants (such as ascorbic acid, gentisic acid or para-aminobenzoic acid), an antimicrobial preservative, an organic solvent (such as ethanol) or filler. The radiopharmaceutical, as defined herein, is typically administered in an amount to provide a dependable image considering the nature of the disease or condition being detected, the patient's weight, size, Waist-Hip Ratio (WHR), Body Surface Area (BSA), Body Mass Index (BMI) and such other factors as would be apparent to a person skilled in the art.
As a general rule to be applied for the radiopharmaceutical, as defined herein, the aim is to have the lowest quantities of excipients possible that produce a pharmaceutically effective as well as physiologically tolerable radiopharmaceutical formulation.
The radiopharmaceutical, as defined herein, contains, inter alia, a suitable amount of the radiolabeled compound of the general Formula (I). For use in positron emission tomography (PET) the amount is between about 5 to between about 20 mCi, preferably 10 to 15 mCi. For use in single photon emission computed tomography (SPECT) the amount is between about 0.1 to between about 30 mCi, preferably 10 to 20 mCi.
In some embodiments, the radiopharmaceutical, as defined herein, is for use in the diagnosis of a disease or disorder.
In other embodiments, the radiopharmaceutical, as defined herein, is for use in risk stratification.
In some embodiments, the disease or disorder is selected from a cardiac disease, renal, neuronal and neoplastic diseases or disorders.
As used herein, the term “cardiac disease” refers to any disease that involves the heart or blood vessels (e.g. coronary artery disease) including diseases related to atherosclerosis (arterial disease). In some embodiments, the disease or disorder is a coronary artery disease.
As used herein, the term “coronary artery disease” refers to any disease or disorder which involves the complete or partial blockage of the blood vessels that supply blood and oxygen to the heart and/or which may involve the symptoms of chest heaviness, dyspnea, fatigue, chest pain, angina or myocardial infarction. Coronary artery disease according to the present invention refers to the entire spectrum of coronary artery diseases.
The present invention also provides the use of at least one compound of Formula (I), as defined herein, for the preparation of a radiopharmaceutical.
In a further of its aspects, the present invention provides the use of at least one compound of Formula I, as defined herein, or of at least one radiopharmaceutical, as defined herein, in the preparation of a composition.
In some embodiments, the composition is a diagnostic composition.
As defined herein the term “diagnostic composition” refers to a composition which is suitable for use as a biomarker for diagnosis of various disease and disorders in human and non-human subjects using molecular imaging. In some embodiments, the molecular imaging is a non-invasive molecular imaging technique selected from myocardial perfusion imaging (MPI), Positron Emission Tomography (PET) or Single Photon Emission Computed Tomography (SPECT) and other methods of non-invasive molecular imaging techniques known to a person skilled in the art.
In some embodiments, the molecular imaging technique is PET.
In some embodiments, the diagnostic composition is for use in the diagnosis of a disease or disorder, as defined herein.
In some embodiments, an additional noninvasive imaging modality (e.g. stress echocardiography) is used sequentially or concurrently with the methods of MPS, PET or SPECT in which the diagnostic composition, compound of Formula (I) or radiopharmaceutical, as defined herein, are used.
The diagnostic composition, as defined herein, may be used for human or animal purposes. The term “animal” refers to a non-human animal.
The diagnostic composition may comprise at least one compound of the Formula (I), as defined herein, and/or at least one radiopharmaceutical, as defined herein, and at least one further pharmaceutically acceptable carrier, diluent, excipient, preservative, solubilizer, emulsifier, and/or adjuvant. The diagnostic composition may be a liquid or lyophilized or otherwise dried formulation and include diluents of various buffer content (e.g.; Tris-HCL, acetate, phosphate), pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween 20, Tween 80, Pluronic F68, bile acid salts), organic solvents (e.g. ethanol), solubilizing agents (e.g., glycerol, polyethylene glycerol), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., Thimerosal, benzyl alcohol, parabens), bulking substances or tonicity modifiers (e.g., lactose, mannitol), covalent attachment of polymers such as polyethylene glycol to the protein, complexation with metal ions, or incorporation of the material into or onto particulate preparations of polymeric compounds such as polylactic acid, polglycolic acid, hydrogels, etc, or onto liposomes, microemulsions, micelles, unilamellar or multilamellar vesicles, erythrocyte ghosts, or spheroplasts. Such compositions will influence the physical state, solubility, stability, rate of in vivo release, and rate of in vivo clearance. Controlled or sustained release compositions include formulation in lipophilic depots (e.g., fatty acids, waxes, oils).
The diagnostic composition may typically comprise at least one radiolabeled compound according to the invention, namely at least one 11C, 18F, 15O or 13N radiolabeled tracer. The selection of the appropriate tracer as a biomarker for diagnosis, particularly one which employs PET, and to some extent the amount of the biomarker, depends on a variety of factors which are known to a person skilled in the art. In some embodiments, the diagnostic composition comprises a single biomarker (tracer). In other embodiments, the diagnostic composition comprises at least two such biomarkers, one of which may or may not be a biomarker according to the invention.
In some other embodiments, the diagnostic composition, as defined herein is used alongside tracers radiolabeled with a different tracer than the one used in the diagnostic composition of the invention.
In some embodiments, the diagnostic composition is used in the diagnosis of a cardiac disease, as defined herein.
In some embodiments, the diagnostic composition is used in the prognosis and risk stratification of cardiac disease in patients with known or suspected cardiac disease.
In other embodiments, the diagnostic composition is used in the prediction of functional recovery following acute myocardial infarction.
In yet other embodiments, the diagnostic composition is used in the prediction of functional recovery after revascularization in patients with chronic ischemic left ventricle (LV) dysfunction.
In accordance with the present invention, the diagnostic composition may be used to determine blood flow to the heart muscle, for determining the effects of a heart attack, or myocardial infarction, on areas of the heart, to identify areas of the heart muscle that would benefit from a procedure such as angioplasty or coronary artery bypass surgery (in combination with a myocardial perfusion scan) or to map normal heart function.
The diagnostic composition, radiopharmaceutical or compound of the invention may be used either in vivo or in vitro for the purposes defined herein.
The diagnostic composition, radiopharmaceutical or compound of the invention may be used in the preparation of kits suitable for use in the diagnosis of a disease or disorder as defined herein.
In a further of its aspects the present invention provides a method for the diagnosis of a disease or disorder, said method comprising administering to a subject (human or non-human) suffering from or having predisposition to suffer from or having symptoms which may be associated with the disease or disorder, a diagnostically effective amount of a compound of Formula (I), as defined herein, or a radiopharmaceutical as defined herein or a diagnostic composition as defined herein, and imaging the body or any part of the body of said subject.
In some embodiments, the disease or disorder is coronary artery disease, as defined herein.
As use herein the term “diagnostically effective amount” refers to an amount of the compound, radiopharmaceutical or diagnostic composition, as defined herein, which allows for efficient molecular imaging depending on the type of the imaging technique (e.g. PET, SPECT etc) used, the acquisition parameters of the specific imaging technique used, the area of the body scanned, the physical condition of the subject, the purpose of the test or any other factors which are apparent to the person skilled in art.
In a still further aspect, the present invention provides a method of monitoring treatment of a subject, said method comprising administering to a subject having disease or disorder a compound as defined herein or a radiopharmaceutical, as defined herein, or a diagnostic composition, as defined herein, and measuring at least one imaging parameter associated with the disease or disorder in the subject using imaging of the body or any part of the body of said subject.
As used herein, the term “imaging parameter” refers to any parameter which can be measured using an imaging technique such as MPI, PET or SPECT as applied to the body or to any part of the body. Examples of such parameters include, inter alia, images (e.g. four-dimensional images or pictures of functional processes in the body) acquired by a nuclear medicine technician, a radiographer or a cardiac technician which correlate with myocardial vascular resistance, the extent of blood supply to the heart muscle (e.g. pointing to inadequate blood supply in specific regions of the heart), information about the heart's pumping function, the amount of scarring from a heart attack, the success of coronary bypass surgery or angioplasty.
A further method is provided, said method being for determining the severity of a disease or disorder, in a subject, the method comprising:
(i) obtaining at least one imaging parameter following the administration of at least one radiolabeled compound, as defined herein, or at least one radiopharmaceutical, as defined herein, or at least one composition, as defined herein, to a subject suffering from the disease or disorder,
(ii) comparing the at least one imaging parameter obtained in (i) with a control reference that correlates with the severity of the disease or disorder, wherein the comparison allowing the determination of the severity of the disease or disorder in the subject.
In a further aspect, the present invention provides a method for determining the effectiveness of a therapeutic treatment of a disease or disorder in a subject, said method comprising:
(i) obtaining at least one imaging parameter following the administration, at a first time point, at least one radiolabeled compound, as defined herein, or at least one radiopharmaceutical, as defined herein, or at least one composition as defined herein, to the subject suffering from a disease or disorder,
(ii) comparing the at least one imaging parameter obtained in (i) with at least one imaging input parameter obtained, following the administration, at a second time point, of at least one radiolabeled compound as defined herein or at least one radiopharmaceutical as defined herein or at least one composition as defined herein, to the subject suffering from the disease or disorder, and wherein the comparison allowing the determination of the effectiveness of a therapeutic treatment.
When PET is used for imaging, a dedicated PET or PET/CT scanner may be used. However, in accordance with the present invention it is also possible to acquire PET images using a conventional dual-head gamma camera fitted with a coincidence detector.
In some other embodiments, the methods of the invention are employed for determining the site of disease. In other embodiments, the methods of the invention are employed for measuring or quantifying or studying pharmacokinetics, biodistribution and drug interactions.
In some embodiments, the radiolabeled compound of the invention is conjugated to at least one drug; the uptake of the conjugate, the tissues in which it concentrates, and its eventual elimination, may be monitored using a molecular imaging technique such as MPI, PET or SPECT, as explained herein.
The present invention further provides methods for the synthesis of compounds of the invention, methods for the preparation of compositions of the invention, methods of administering to a subject of compositions of the inventions.
In order to understand the invention and to see how it may be carried out in practice, numerous examples are provided and should be considered as non-limiting of the invention as disclosed.
The present invention is based on the finding that radiolabeled ammonium salt derivatives can be used for the imaging of myocardial blood flow. They are positively charged, enhancing their tendency to undergo renal rather than hepatic excretion and increasing their tendency for cardiac accumulation. In addition, their lipophilicity can be easily manipulated via their functional groups and their labeling with carbon-11 is simple, straightforward and easily automated. Furthermore, carbon-11's half-life (20.4 min) provides adequate time for chemical transformations and an option for delivery to surrounding PET centers while keeping the waiting period between consecutive scans to less than two hours, even for equal doses.
The labeled ammonium derivatives of the invention were synthesized and evaluated as myocardial blood flow PET imaging agents. [11C]-2 gave high accumulation and retention in the heart, undergoing fast clearance mainly via the kidneys and yields high % ID/g heart ratios versus organs that interfere with cardiac imaging (e.g., blood, liver and lungs). In addition, since most tracers of this type are excreted via the kidneys and not the liver, the likelihood of gut activity (a well-known source of serious image quality problems that occur while using 99mTc-labled lypophilic cations) interfering with myocardial imaging is minimal. [11C]-3 gave superior cardiac accumulation, but its slower washout profile from the bloodpool, and lungs may pose a problem as far as resolution and patient dosimetry are concerned. [11C]-4 gave the best cardiac uptake and washout profiles with heart/tissue ratios improving over time and giving the best overall results.
The biodistribution data for [11C]-1 (log P=−3.92)[35] indicates little, if any, accumulation of tracer in the target tissue. Substitution of one methyl group in [11C]-1 to the more lipophilic phenyl group in [11C]-2 (log P=−2.74) [17], significantly increased cardiac activity uptake. This small, yet important, change in chemical structure increased cardiac uptake with minimal changes of the biodistribution profile to other organs. After lipophilicity was shown to play a major role in tracer uptake, the issue of increased positive charge was examined. The incorporation of the para-dimethylamino group into [11C]-2 resulted in [11C]-3-(log P=−0.93±0.1, n=3, data not shown). In addition, [11C]-3 accumulated in the lungs to a higher degree, increasing background radioactivity. The switch of a second methyl with a phenyl in [11C]-4 increased cardiac uptake but, more importantly, decreased cardiac washout to almost nothing, increasing heart/tissue ratios over time.
The results obtained with [99mTc]MIBI correlate with those from earlier publication (
The results of the PET/CT experiments in rats are consistent with the biodistribution results in mice. [11C]-1 gave little cardiac accumulation while [11C]-2 and [11C]-3 both accumulated in the heart, indicating, once again, the potential of [11C]-2 as a myocardial blood flow PET tracer. Accumulation of [11C]-3 in the lungs and liver was higher than that observed with [11C]-2 while the differences in cardiac washout profiles were negligible.
Materials and Methods
All solvents were of analytical grade. Dry acetonitrile was purchased from Merck (Darmstadt, Germany). Trimethylamine in THF 1M was purchased from Acros (Geel, Belgium). LiAlH4 in THF (0.25M) was purchased from ABX (Radeberg, Germany).
Radiosynthesis was carried out on an automated module (GE, Munster Germany). 11C-carbon dioxide was produced by the 14N(p,α)11C nuclear reaction on nitrogen containing 0.5% oxygen, using an 18/9 IBA cyclotron. At EOB, the target gas was delivered and trapped by a cryogenic trap in the [11C]CH3I module.
Radiochemical purity, chemical purity and identification of [11C]-1 was performed on a Varian 9012Q HPLC system with an Alltach 550 conductivity detector and a PMT/Scintillator detector Bioscan flow count. A cation exchange IC-PAK™ Cation M/D 150*3.9 mm (Waters Corporation, Milford, Mass.) column was used with 5 mM HCl as eluent at a flow rate of 1.2 mL/min.
For the purification and analysis of [11C]-2, two HPLC systems were used: (A) a reversed-phase system employing a Machery-Nagel 100-7 C-18 column (250×16 mm) and 15% EtOH/85% acetate buffer 0.1 M/pH 3.8 as eluent, at a flow rate of 8 mL/min which was installed in the [11C]CH3I module. (B) Analysis of formulated radiotracer was performed on the same Varian HPLC system as for [11C]-1 employing a Varian 9050 UV-VIS detector instead of a conductivity detector and using a Waters μBondapak C-18 column (10 μm, 125 A, 300×3.9 mm) with 42% CH3CN/58% acetate buffer 0.1 M/pH 3.8 as eluent at a flow rate of 1 mL/min.
For the purification and analysis of [11C]-3, two HPLC systems were used: (C) a reversed-phase system employing the same preparative column as for [11C]-2 with 30% EtOH/70% phosphate buffer 0.1M/pH 5.5 as eluent, at a flow rate of 8 mL/min which was installed in the [11C]CH3I module. (D) Analysis of formulated radiotracer was performed on the same Varian HPLC system with the same column as for [11C]-2 with 85% CH3CN/15% acetate buffer 0.1 M/pH 3.8 as eluent at a flow rate of 1 mL/min.
The purification of [11C]-4 was performed in a similar manner to that of [11C]-2 and analysis was done on the same equipment setup but the eluent used was a mixture of CH3CN and acetate buffer 0.1 M/pH 3.8 with a gradient of 10-20% CH3CN over a period of 10 minutes followed by another 10 minutes of 20% CH3CN.
For the purification and analysis of [11C]-6, two HPLC systems were used with the same equipment and conditions used for the synthesis of the labeled trimethyl-phenyl-ammonium iodide ([11C]-2). The retention time (RT) of [11C]-6 on preparative HPLC was 13 min and on analytical HPLC RT was 8.6 min. For the purification and analysis of [11C]-7 and [11C]-4, the same two systems as for [11C]-2 were used with modifications made only to the eluents. Both compounds were purified on a preparative HPLC system with 15% EtOH/85% acetate buffer 0.1 M/pH 3.8 with a flow rate of 13 mL/min and RT=14-16 min. Both compounds were analyzed using a gradient of 10-20% acetonitrile/90-80% acetate buffer 0.1 M/pH 3.8 over 25 min, followed by 80% acetonitrile/20% acetate buffer for another 5 min. Flow was 1 mL/min for both compounds with [11C]-7 and [11C]-4 having a RT of 20 min and 22 min, respectively.
UV-VIS detectors were set to a wavelength of 254 nm in all the HPLC procedures.
Evaluation of THF, acetonitrile and ethanol traces in the final product solution was performed on a Varian Star 3400 CX gas chromatograph system with a TCD detector, a Bondapak Q80/100, 8 inch column and a helium gas phase at a flow rate of 40 mL/min.
BALC/c Ola/Hsd male mice (7-8 weeks, 25-30 g), SD/Hsd male rats weighing 250-275 g and NZW male rabbit weighing approximately 3.7 Kg were obtained from Harlan Industries, Inc. All animal studies were conducted under a protocol approved by the Animal Research Ethics Committee of the Hebrew University of Jerusalem and in accordance with its guidelines Animals were routinely kept in 12-h light/dark cycles and provided with food and water ad libitum.
Chemistry
Trimethyl-phenyl-ammonium iodide (Compound 2)
N′N′-Dimethylaniline (500 mg, 4.12 mmol) was dissolved in dichloroethane (2 mL) and methyl iodide (880 mg, 6.2 mmol) was added dropwise. The reaction was refluxed for 1 hour during which the product sedimented. The reaction was cooled to room temperature, the solid was filtered out, washed with dichloromethane and dried overnight in a desiccator to give 505 mg (90% yield). 1H NMR (DMSO-d6): δ7.95 (d, J=8.1, 2H), 7.5-7.7 (m, 3H), 3.59 (s, 9H); MS (ESI, m/z): 136 (M+).
(4-Dimethylamino-phenyl)-trimethyl-ammonium iodide (Compound 3)
N,N,N′,N′-Tetramethyl-benzene-1,4-diamine (200 mg, 1.22 mmol) was dissolved in dichloroethane (2 mL) and methyl iodide (173 mg, 1.22 mmol) was added dropwise. The reaction was refluxed for 1 hour, cooled to room temperature, the solid filtered out, washed with dichloromethane, dissolved in methanol and purified via flash column chromatography using a gradient of 0-8% MeOH:DCM to give 260 mg of grey solid (70% yield). 1H NMR (DMSO-d6): δ7.67 (d, J=9.3, 2H), 6.77 (d, J=9.3, 2H) 3.49 (s, 9H), 2.92 (s, 6H); MS (ESI, m/z): 179 (M+).
Dimethyl-diphenyl-ammonium iodide (Compound 4)
Methyl-diphenyl-amine (756 mg, 4.13 mmol) was dissolved in dichloroethane (2 mL) and methyl triflate (1017 mg, 6.2 mmol) was added dropwise. The reaction was refluxed for 2.5 hours and the solvent was evaporated to give a white solid which was purified via flash column chromatography using a gradient of 0-2% MeOH:DCM to give 1237 mg of white solid (yield 92%). 1H NMR (DMSO-d6): δ7.5-7.7 (m, 10H), 4.06 (s, 6H); MS (ESI, m/z): 198 (M+).
Trimethyl-m-tolyl-ammonium iodide (Compound 6)
Dimethyl-m-tolyl-amine (556 mg, 4.12 mmol) was dissolved in dry dichloroethane (2 mL) and methyl iodide (880 mg, 6.2 mmol) was added dropwise. The reaction was refluxed for 1 h during which time sedimentation occurred. The reaction was cooled to room temperature, the solid filtered out, washed with dichloromethane, and dried overnight in a desiccator to give a white powder (809 mg, 72% yield). 1H NMR (DMSO-d6): δ7.82 (s, 1H), 7.74 (d, J=8.5 Hz, 1H), 7.51 (t, J=8 Hz, 1H), 7.93 J=7.5 Hz, 1H), 3.59 (s, 9H), 2.42 (s, 3H); MS (ESI, m/z): 150 (M+).
Trimethyl-naphthalen-1-yl-ammonium trifluoro-methane-sulfonate (Compound 7)
Dimethyl-naphthalen-1-yl-amine (705 mg, 4.12 mmol) was dissolved in dry dichloroethane (2 mL) and methyl triflate (880 mg, 6.2 mmol) was added dropwise. The reaction was refluxed for 2 h while the solution turned dark brown. The reaction was cooled to room temperature, dichloroethane replaced with dichloromethane and diethyl-ether added to cause precipitation. Precipitate was collected, washed with ether and dried in a desiccator to give a white powder (1.36 g, 94% yield). 1H NMR (DMSO-d6): δ8.59 (d, J=9, 1H), 8.21 (t, J=10 Hz, 2H), 8.09 (d, J=8 Hz, 1H), 7.78 (t, J=7 Hz, 1H), 7.71 (t, J=7 Hz, 1H), 7.66 (d, J=9.5 Hz, 1H), 3.89 (s, 9H); MS (ESI, m/z): 186 (M+).
Compound [11C]-1
Carbon-11 MeI was prepared as follows: [11C]CO2 (44 GBq, 1,000 mCi) was trapped at −160° C. The temperature of the cooling trap was increased to −20° C., and the activity was transferred by a stream of argon (40 mL/min) into the first reactor containing 300 μL of 0.25 N LiAlH4 in THF at −50° C. After 90 s, the solvent was removed under reduced pressure. In this manner, approximately 66% of the activity was recovered. The reactor temperature was increased to 160° C., HI was added and [11C]MeI was distilled (argon flow of 15 mL/min) through a NaOH column to the second reactor, containing trimethylamine 1 M in THF (0.3 mL, 0.3 mmol) and 300 μL of acetonitrile. After 1 min of distillation, an average of 493±46 mCi (n=4) was trapped in the second reactor at −15° C. The reactor was sealed and heated to 65° C. for 5 minutes and then the reaction temperature was raised to 80° C. under vacuum for one minute in order to evaporate volatiles. The crude product was diluted with water (2 mL) and transferred to a round bottomed flask containing more water (8 mL). This solution was loaded onto a Light CM cation exchange Sep-Pak (Waters Corporation, Milford, Mass.), washed with water (17 mL), dried, and the product was eluted with saline (5 mL) to give 304±47 mCi. Radiochemical yield was 55±8% decay corrected to EOB with a total synthesis time of 25 min, a specific activity of 3.6±0.5 Ci/μmole and radiochemical purity higher than 99% (n=4).
Compound [11C]-2
[11C]MeI was produced as described for [11C]1 and distilled to the second reactor, containing N′,N′-dimethylaniline (20 μL, 0.17 mmol) and 280 μL of acetonitrile. After 1 min of distillation, an average of 505±90 mCi (n=4) was trapped in the second reactor at −15° C. The reactor was sealed and heated to 80° C. for 5 minutes. At the end of the 5 minute reaction, the reaction temperature was raised to 100° C. under vacuum for one minute in order to evaporate volatiles. The crude product was diluted with EtOH:acetate buffer (7:3, 2 mL) and automatically injected to the HPLC (system A), rt=8 minutes. The labeled product (80±1.6 mCi) was collected in a flask and diluted with saline to give a final solution of the tracer in 1:9 EtOH:acetate buffer (pH 3.8). Radiochemical yield was 19%±1.6 decay corrected to EOB with a total synthesis time of 35 min, specific activity of 2.5±0.3 Ci/μmole and radiochemical purity higher than 99%.
Compound [11C]-3
[11C]MeI was produced as described for [11C]-1 and distilled to the second reactor, containing N,N,N′,N′-tetramethyl-benzene-1,4-diamine (20 mg, 0.12 mmol) and 280 μL of acetonitrile. After 1 min of distillation, an average of 500±60 mCi (n=4) was trapped in the second reactor at −15° C. The reactor was sealed and heated to 80° C. for 5 minutes. At the end of the 5 minute reaction, the reaction temperature was raised to 100° C. under vacuum for one minute in order to evaporate volatiles. The crude product was diluted with water (2 mL) and automatically injected to the HPLC (system C), rt=13 minutes. The labeled product (150±31 mCi) was collected in a flask and a sample was diluted with saline to give a final solution of the tracer in 1:9 EtOH:phosphate buffer (pH 5.5). Radiochemical yield was 52%±11 decay corrected to EOB with a total synthesis time of 40 min, specific activity of 3.3±0.5 Ci/μmole and radiochemical purity higher than 99%.
Compound [11C]-4 Trifluoro Methane Sulfonate Salt
[11C]MeOTF (53±3 GBq (n=3)) was trapped in a reactor containing methyl-diphenyl-amine (20 μL) and dry 2-butanone (280 μL) at −15° C. The reactor was sealed and heated to 80° C. for 5 min. At the end of the 5 min reaction, the reaction temperature was raised to 90° C. under vacuum for 1 min in order to evaporate volatiles. The crude product was diluted with an ethanol/acetate buffer (7:3, pH 3.8, 0.1 M) solution (2 mL) and automatically injected to the HPLC, RT=14 min. The labeled product (1.96±0.37 GBq) was collected in a flask and a sample was diluted with saline to give a final solution of the tracer in 1:9 EtOH/phosphate buffer (pH 5.5). Radiochemical yield was 13%±2% decay corrected to EOB with a total synthesis time of 36 min, specific activity of over 260 GBq/μmol, and radiochemical purity higher than 99%.
Compound [18F]-5
An [18O]H2O/[18F−] (29±4 GBq, n=4) mixture was trapped and transferred to a reactor through an ion exchange column (preactivated with 0.8 mL of EtOH and 3 mL of HPLC water) by elution with 0.5 mL of potassium carbonate (6 mg/mL). Following the addition of Kryptofix 2.2.2 solution (15 mg, 40 μmole in 1 mL CH3CN), azeotropic removal of water and acetonitrile was achieved by heating the reactor to 100° C. under a stream of nitrogen for 3 min and under vacuum for another 1 min. Methanesulfonic acid 2-diphenylamino-ethyl ester (10 mg, 34 μmole) dissolved in CH3CN (1 mL) was added, the reactor temperature was increased to 120° C. and the reaction mixture was stirred for 20 min. Volatiles were evaporated at the same temperature After cooling the mixture to under a stream of nitrogen for 1 min and under vacuum for another 2 min. The reactor was cooled to 50° C. and then 2,6-di-tert-butyl-4-methylpyridine (92 mg, 0.45 mmol) in dry dichloromethane (400 μL) and methyl trifluoro-methanesulfonate (50 μL, 0.44 mmol) in dry dichloromethane (200 μL) were added. The reactor temperature was increased to 105° C. for 25 min. and then cooled to 45° C.
Non-decay corrected radiochemical yields were approximately 85% and 80% for the first and second steps of the synthesis, respectively.
Purification was performed either via normal phase sep-pak (preactivated with 5 mL dichloromethane) using 2% MeOH in dichloromethane which was evaporated and replaced with saline or by replacing the dichloroethane with 70% EtOH in acetate buffer (pH 3.8, 0.1 M) and purification via preparative HPLC using 10% EtOH in acetate buffer (pH 3.8, 0.1M) as eluent. The product was analyzed using HPLC with a flow of 1 mL/min and a gradient of 30-90% CH3CN (70-10% acetate buffer, pH3.8, 0.1M) over 40 minutes.
Compound [11C]-6
The production of [11C]methyl iodide ([11C]MeI) was described in detail in the past.3, 11 20±1.5 GBq (n=4) of [11C]MeI were trapped in a reactor containing dimethyl-m-tolyl-amine (20 μL) and dry acetonitrile (280 μL) at −15° C. The reactor was sealed and heated to 80° C. for 5 min. At the end of the 5-min reaction, the reaction temperature was raised to 90° C. under vacuum for 1 min in order to evaporate volatiles. The crude product was diluted with an ethanol/acetate buffer (7:3, pH 3.8, 0.1M) solution (2 mL) and automatically injected into the HPLC RT=13 min. The labeled product (3.1±0.36 GBq) was collected in a flask and a sample was diluted with saline to give a final solution of the tracer in 1:9 EtOH/phosphate buffer (pH 5.5). Radiochemical yield was 27%±3 decay corrected to EOB with a total synthesis time of 37 min, specific activity of 100±11 GBq/μmol, and radiochemical purity higher than 99%.
Compound [11C]-7
[11C]MeI was distilled through silver trifluoro-methane-sulfonate column heated to 200° C. using a Helium flow of 15 mL/min and [11C]MeOTf (21±1 GBq (n=3)) was trapped in a reactor containing N′N′-dimethyl-1-naphtylamine (20 μL) and dry 2-butanone (280 μL) at −15° C. The reactor was sealed and heated to 80° C. for 5 min. At the end of the 5-min reaction, the reaction temperature was raised to 90° C. under vacuum for 1 min in order to evaporate volatiles. The crude product was diluted with an ethanol/acetate buffer (7:3, pH 3.8, 0.1 M) solution (2 mL) and automatically injected into the HPLC, RT=16 min. The labeled product (1.6±0.24 GBq) was collected in a flask and a sample was diluted with saline to give a final solution of the tracer in 1:9 EtOH/phosphate buffer (pH 5.5). Radiochemical yield was 14%±2 decay corrected to EOB with a total synthesis time of 40 min, specific activity of over 170 GBq/μmol, and radiochemical purity higher than 99%.
Radiochemistry
Quality Control
Three quality control tests were performed parallel to each other:
A GC injection of 5 μL was performed to ensure a maximal concentration of THF, acetonitrile and ethanol below 720 ppm, 410 ppm and 10% v/v, respectively.
Two HPLC injections were prepared: a 50 μL injection for radiochemical purity and specific activity calculations and a 50 μL co-injection with a non-radioactive standard to insure the tracer's identity. pH was checked as well using a pH strip in the appropriate range of the expected solution.
Animal Studies
Tracer Biodistribution Studies in Mice
Compounds [11C]-1, [11C]-2, [11C]-3, [11C]-4, [11C]-6, [11C]-7 and Technetium-99 m methoxy isobutyl isonitrile ([99mTc]MIBI) biodistribution studies were carried out with BALB/c Ola/Hsd mice (7-8 week). Tracers were injected in a 10% EtOH/saline solution as vehicle. Either [11C]-1 (62±15 μCi; 2.4±6 MBq; 122±13 μL), [11C]-2 (68±12 μCi; 2.5±0.4 MBq; 188±1.3 μL), [11C]-3 (70±17 μCi; 2.6±0.6 MBq; 185±5 μL), [11C]-4 (18±5 μCi; 666±185 MBq; 195±2.2 μL or 1.24±0.93 MBq, 229±63 μL), [11C]-6 (1.95±0.21 MBq, 83±41 μL), [11C]-7 (2.01±0.16 MBq, 169±73 μL) or [99mTc]mibi (14.2±1.7 μCi; 3.6±0.4 MBq; 97.7±12.5 μL) were injected via the lateral vein. At allotted time points (1, 5, 10, 15 min), blood was drawn from the orbital sinus, mice were sacrificed by cervical dislocation and selected organs (heart, lungs, liver and kidney) were excised, weighed and measured for their radioactive content using a γ-counter (1480 Wizard 3″). Distribution of activity was calculated as the percentage of injected dose per gram of organ (% ID/g). Activity uptake ratios of various organs were calculated by dividing the corresponding calculated % ID/gs.
Similar to the biodistribution studies in mice, [11C]-4 was injected into male SD/Hsd rats (293±9 g) in a 10% EtOH/saline solution (3.1±0.5 MBq; 530±134 μL) via the lateral tail vein. At the allotted time points, blood was drawn, animals were sacrificed, and selected organs were excised, weighed and measured for their radioactive content.
Distribution of activity was calculated as the percentage of injected dose per gram of wet tissue (% ID/g). Activity uptake ratios of various organs were calculated by dividing the corresponding calculated % ID/g. Heart standardized uptake values (SUVs) were calculated by dividing the % ID in the heart of each rat by the percentage of heart weight of the total body weight of the animal.
PET/CT Imaging in Rat
All PET/CT studies were carried out with SD/Hsd male rats weighing 250-275 g. Rats were anaesthetized with Ketamine:Xylazine 2% (100 μL/100 g body weight injected i.p). Animals were placed in a supine ([11C]-1) or prone ([11C]-2 and [11C]-3) position on a flat polystyrene foam support, with feet taped to minimize likelihood of movement during the scans. Imaging was performed on a Discovery ST PET/CT tomograph (General Electric, Milwaukee, Wis.), with intrinsic spatial resolution: in plane 6.2 mm, axial 5.0 mm. CT transmission scans started prior to administration of the tracer. PET scans started immediately with the IV administration of the tracer, approximately 300 μCi (11 GBq). Fifty minutes dynamic emission scans were obtained in 2D mode, and corrected for random counts, dead time, scatter and attenuation. Images were reconstructed using iterative reconstruction. Regions of interest (ROI) were drawn on the heart, lungs, liver, kidneys and bladder and time activity curves were calculated for these regions.
Micro-PET scans were also carried out using healthy Wistar rats (154±47 g). Anesthesia was induced, and thereafter maintained by 3% and 1%-2.5% isoflurane in O2, respectively. During the scans, rats were maintained normothermic using a heating blanket. All CT and PET acquisitions were carried out using the Inveon™ multimodality PET-CT small animal-dedicated scanner (Siemens Medical Solutions USA Inc.). Rats were positioned in the scanner so that the heart was in the center of the field of view. Following a CT scan, PET list-mode acquisition was started at the time of injection of [11C]-4 (14.3±5.7 Mbq/rat, n=4) or [13N]—NH3 (81.1±20.4 Mbq/rat, n=6) in the lateral tail vein. Acquisitions continued for 60 min or 40 min post-injection, for [11C]-4 or [13N]—NH3, respectively.
The list-mode emission scans were sorted into 29 (6×10, 8×30, 5×60, 10×300 sec) and 24 (12×10, 6×30, 5×300, 1×600 sec) dynamic frames, for [11C]-4 and [13N]—NH3 respectively. Emission sinograms were normalized and corrected for attenuation, dead time and decay. Image reconstruction was performed using Fourier rebinning and two dimensional ordered-subsets expectation maximization (2D-OSEM), with a voxel size of 0.776×0.776×0.796 mm3.
Image analysis and quantification of radioactivity concentration in regions of interest (ROIs) were performed using Inveon Research Workplace 3.1 (Siemens Medical Solutions USA Inc.). Delineation of ROIs was performed by manual segmentation, and the corresponding time activity curves (TACs) were calculated.
PET/CT Studies of [11C]-4 in a Porcine Model
Study Protocol
Following a constriction of the left circumflex coronary artery (LCxA) via insertion and manipulation of a stent, stress and rest dynamic-PET studies were performed in three pigs. Pigs underwent dynamic PET acquisition following injections of [11C]-4 both at rest and under pharmacological stress [(207±30 MBq in 10±4 mL) and (203±11 MBq in 16±1 mL), respectively]. Images were acquired for each animal at rest and stress on the same day, allowing a minimum of 5 half-lives (approximately 2 h for carbon-11 and 1 h for nitrogen-13) in between injections to minimize crosstalk. Pharmacological stress was induced by an IV infusion of adenosine (500 μg×min−1×kg−1 body weight), beginning 3 min prior to injection of the tracers, and maintained for an additional 3 min. When necessary, hypotension was prevented by concomitant IV administration of dobutamine (2 μg×min−1×kg−1 body weight).
In the first two pigs, myocardial infarction developed as evidenced by either ECG monitoring or repeated angiography that demonstrated total stent occlusion. In order to prevent complete occlusion in the third pig, the angiography and stent insertion were carried out in the PET/CT room, thus minimizing the animal's movement, and the animal was given higher doses of heparin (a sum of 30,000 units throughout the experiment) based on Activated Clotting Time (ACT) test. In the third pig, the adenosine stress protocol was performed twice: once with [13N]NH3 (700 MBq in 1 mL) injection, and 2.5 h later, the adenosine stress protocol was repeated with [11C]-4 injection.
Animal Preparation
To prevent blood clotting, pigs were treated with aspirin (250-300 mg) and plavix (150-300 mg) on the day before the procedure. On the day of the study, the animals were anesthetized using ketamine/xylazine (15 mg/kg/2 mg/kg) and Butorphanol 0.2 mg/kg IM as premedication. For induction, ketamin/diazepam (4 mg/kg/0.02 mg/kg) was administered intravenously. Animals were ventilated and anesthesia, maintained using isoflorane 2% inhalation (via tracheal tube). Animals were monitored continuously for ECG, body temperature, O2 saturation, blood pressure, pulse and breathing. Vascular access was prepared in the left and right ear veins for drug and tracer administration, and a catheter was placed in the left femoral artery for stent insertion and arterial blood pressure measurements.
Partial Occlusion of the LCx Coronary Artery
Following anesthesia, the pigs underwent cannulation of the left femoral artery with a 7 Fr introducer sheath and a 7 Fr hockeystick catheter (Cordis) was positioned in the left main coronary artery. Partial LCx coronary artery occlusion was induced via partial inflation of the proximal component of a JOSTENT Graftmaster (Abbott Vascular, USA) inside the LCx coronary artery, creating a funnel-shaped structure. Before insertion of the stent, the 3.5×16 mm JOSTENT was partially crimped over a 3.5 mm balloon with its distal third beyond the inflating balloon. Inflation of the balloon dilates the crimped part leaving a distal cone that is not dilated with an orifice approximately 1 mm in diameter. Thus, when deployed in an artery that is 3.5 mm in diameter, a partial, yet significant stenosis was established.
Coronary angiography was performed with 20-30 mL of contrast media and 3.5 mm wide and >20 mm long segment of the LCx artery that was free of major branches. The stent system was brought to the assigned segment over a floppy 0.014 coronary wire (ASAHI). The balloon was inflated with a pressure of 14-16 atmospheres (10-15 seconds) and the balloon was then deflated and withdrawn. Angiography was then performed in two different projections to ascertain that the cone-shaped stent enabled preserved continuous flow through its patent distal orifice. To ensure partial stenosis rather than complete occlusion of the artery during the PET imaging, a repeated angiography was taken at the end of imaging.
PET/CT Acquisition
Animals were placed in a supine position with the heart in the center of the field of view of the PET/CT scanner. Imaging was performed on a Discovery ST PET/CT tomograph (GE Healthcare, Milwaukee, Wis.), with intrinsic spatial resolution: in-plane 6.2 mm, axial 5.0 mm. CT transmission scans were carried out prior to the administration of the tracer. Dynamic emission scans starting 10-15 seconds before tracer injection (4×5 secs, 15×3 secs, 5×5 secs, 7×30 secs, 2×2.5 mins, 6×5 min: 40 minutes total acquisition time for [11C]-4; 12×10 secs, 6×30 secs, 3×5 mins: 20 mins total acquisition time for [13N]NH3) were acquired in a two-dimensional mode and corrected for random counts, dead time, scatter, and attenuation.
PET Data Analysis
Images were reconstructed using OSEM iterative reconstruction. The PET images were then visualized and analyzed using the Cardiac Image Analysis System Carimas 2.0 (Turku PET Centre, Turku Finland: http://www.turkupetcentre.net/carimasturku/) software. Using this software package, the long axis of the left ventricle is defined in transaxial slices through the heart; the heart is reoriented to the standard short axis projection, and volumes of interest (VOIs) are defined semi-automatically for the entire left ventricle and for the territories of the main blood vessels as well as smaller subdivisions, and for a blood pool region toward the base of the left ventricle. Time activity curves (TACs) were calculated for all these VOIs. In addition, VOIs for the right ventricle, and lungs were drawn on images obtained by summing all time frames from 10 min post-injection to the end of the scan, using IDL (Research Systems, Inc.). TACs were calculated for these VOIs as well. Myocardial tracer activity ratios between the myocardium (lateral wall) and the lung, and left ventricular blood pool were calculated.
In order to create standard stress and rest images, the dynamic data between 5 and 20 min of each imaging set were temporally summed for each reconstructed transaxial slice. Each transaxial set of images was processed with the help of commercial myocardial perfusion viewing software (Myovation-LVSD-GE Healthcare) in order to create short-axis, vertical long-axis and horizontal long-axis myocardial slices as well as corresponding polar maps.
Results
Chemistry
Both trimethylamine and tetramethylammonium iodide were purchased from commercial sources. Trimethyl-phenyl-ammonium iodide (2) was synthesized using N′N′-dimethylaniline and methyl iodide in dichloroethane (90% yield), requiring no purification procedure other than filtration and washing with dichloromethane. (4-Dimethylamino-phenyl)-trimethyl-ammonium iodide (3) was synthesized using N′,N′,N,N-tetramethyl-benzene-1,4-diamine and methyl iodide in dichloroethane and purified using flash column chromatography (70% yield). Dimethyl-diphenyl-ammonium iodide (4) was synthesized using methyl-diphenyl-amine and methyl triflate in dichloroethane and purified using flash column chromatography (90% yield).
The issue of charge density was investigated by adding a methyl group on the meta position on the phenyl ring of compound 2 to give compound 6. In order to investigate lipophilicity, the phenyl ring of compound 2 was replaced with a naphthalene moiety, producing compound 7. Since some of the lipophilic cationic tracers for MPI are based on a cationic core surrounded by lipophilic side chains, one of the methyl groups in compound 2 was replaced with a phenyl yielding compound 4. Ultimately, compounds 7 and 4 differ not only in their Log P values, but also in the distribution pattern of the lipophilicity around the cationic core.
Compound [11C]-6 was synthesized in a similar manner to that of [11C]-2 and gave a slightly improved decay corrected radiochemical yield (27%, EOB). Compounds [11C]-7 and [11C]-4 were synthesized employing the carbon-11 methylation reagent [11C]MeOTf. Radiochemical yields for both compounds were significantly lower than those of the other ammonium tracers (14% and 13% for [11C]-7 and [11C]-4, respectively).
Radiochemistry
[11C]-1 Synthesis
The synthesis of [11C]-1 was based on the labeling conditions of [11C]choline [30] with minor modifications. The methylation reaction was carried out in a THF/CH3CN mixture (1:1) at 65° C. for 6 minutes. Volatiles were evaporated; the product was diluted with water, loaded onto a cation exchange Sep-Pak and then eluted with saline. This 25-minute radiosynthesis gave 304±47 mCi of [11C]-1 with a radiochemical yield of 55% (decay corrected to EOB), specific activity of 3.6±0.65 Ci/μmole and 99% radiochemical purity. (n=4)
[11C]-2 Synthesis
The methylation reaction to obtain [11C]-2 was performed in CH3CN at 80° C. for 5 minutes. Volatiles were evaporated, a solution of ethanol:acetate buffer (7:3) was added and the product purified using RP-HPLC (system A). This 35-minute radiosynthesis gave 80±1.6 mCi [11C]-2 with a radiochemical yield of 19%±0.16 (decay corrected to EOB) a specific activity of 2.5±0.3 Ci/μmole and radiochemical purity of 99% (n=3).
[11C]-3 Synthesis
The methylation reaction to obtain [11C]-3 was performed as described for [11C]-2 but after volatile evaporation, water was added and the product was purified using RP-HPLC (system C). This 40-minute synthesis gave 150±31 mCi of [11C]3 with a radiochemical yield of 52%±11 (decay corrected to EOB) a specific activity of 3.3±0.5 Ci/μmole and radiochemical purity of 99% (n=3).
The methylation reaction to obtain [11C]-4 was performed as described for [11C]2 except for the use of a AgOTf column to produce the methyl triflate rather than methyl iodide for the methylation reaction. This 40-minute synthesis gave 19±2.4 mCi of [11C]3 with a radiochemical yield of 7%±0.8 (decay corrected to EOB) a specific activity of 2.7±0.4 Ci/μmole and radiochemical purity of 99%. (n=3).
Biodistribution
The in-vivo biodistribution of [11C]-1, [11C]-2, [11C]-3, [11C]-4, [11C]-6, [11C]-7 and [99mTc]MIBI was evaluated in mice at different time points post-injection. The biodistribution of radioactivity 1, 5, 10 and 15 minutes post-injection is presented in
For compound [11C]-2 the radioactivity in the heart was the highest among the tissues examined, reaching 29.5%, 26.8%, 18.5% and 13.9% ID/g at 1, 5, 10 and 15 minutes post injection (
[11C]-3 was synthesized with the idea that its increased charge in physiological pH could enhance its cardiac uptake and accelerate renal filtration and excretion. Indeed radioactivity uptake values in the heart increased to 48.7%, 47.7%, 40.15% and 28.5% ID/g at 1, 5, 10 and 15 minutes post injection, respectively (
[11C]-4 was synthesized to evaluate what effects a further increase in log P will have on the cardiac uptake and retention of the ammonium tracer in the heart. The change not only increased heart uptake at all time points to 46.6%, 50.4%, 62.5% and 44.5% at 1, 5, 10 and 15 minutes but decreased radioactivity washout from the heart almost completely. This change did not change liver uptake of the tracer significantly but decreased lung uptake and the activity washout from the blood washout occurred faster. This tracer showed the best heart/blood activity uptake ratios (44 and 61 fold at 5 and 10 minutes respectively)
The four ammonium-based tracers' biodistribution profiles were compared with those of the well known myocardial perfusion imaging tracer [99mTc]MIBI at the same time points. Radioactivity accumulation in the heart was modest, reaching 24%, 20.5% 21.4% and 13.8% ID/g at 1, 5, 10 and 15 minutes post injection, respectively (
[11C]-2 (n=7) and [11C]-6 (n=8) have very similar biodistribution profiles with cardiac % ID/g reaching ˜20% and heart/blood ratios reaching ˜13 at 10 min post injection (results not shown). Despite this similarity, it is evident that at each time point and in almost all parameters, [11C]-6 surpasses [11C]-2. [11C]-6 has lower uptake in the lungs and higher uptake in the heart, leading to improved heart/tissue ratios. [11C]-7 (n=4) has improved heart uptake (˜33% ID/g, 10 min) with little effect on uptake in other organs or blood pool clearance. This provides improved heart/tissue ratios in all tissues, at all time points. Despite these advantages, similarly to [11C]-2, there is still considerable washout of activity from the heart and heart/blood ratios do not exceed 20. [11C]-4 (n=6) gave the best results of all compounds. Its heart uptake was constant at ˜50% ID/g at all observed time points and its blood pool clearance was faster than that of all the other tracers. In addition, although liver uptake was increased, kidney uptake did not change significantly and lung uptake decreased. Due to the minimal washout of tracer from the heart, heart/tissue ratios improved over time, suggesting the feasibility of performing MPI/PET imaging with this compound at later time points. Finally, 4 was compared to the popular SPECT agent, [99mTc]-MIBI. [99mTc]-MIBI (n=4) showed a better blood pool clearance and slightly lower lung uptake. However, heart uptake of [11C]-4 was twice as high, while kidney retention was significantly less than that obtained with [99mTc]-MIBI. [11C]-4 had better heart/tissue ratios for all organs, at all time points. Heart SUV was calculated, and reached a value of 9.7±0.5 at 15 min post injection.
The in vivo distribution of [11C]-4 in rats was evaluated at 5, 10 and 15 min post-injection of the compound (n=3 per time point). As shown in
PET/CT Scans in Rats
The feasibility of cardiac blood flow imaging with the three tracers was evaluated using SD/Hsd male rats Animals were anesthetized, placed under a clinical PET/CT scanner, injected with approximately 300 μCi for rats and imaged for up to 50 minutes.
microPET in Rats
Time activity curves obtained after injection of [11C]-4 (n=4) and [13N]NH3 (n=6) into rats were calculated for organs of interest. As demonstrated in
PET Imaging of Ischemia and Infraction in Pigs Using [11C]-4
For the evaluation of [11C]-4 uptake during ischemia in a large-animal model, pigs rather than dogs and rabbits, were chosen for two main reasons: first, similarities between porcine and human cardiovascular physiology, heart size and anatomy, and the distribution of coronary perfusion make them superior models compared to other species. Second, pigs' coronary arteries are large enough to better enable the catheterization techniques required for our model of partial coronary occlusion. This novel model simulating significant, albeit partial coronary artery occlusion, has clear advantages over other models which rely on either permanent or transient complete occlusions. Our model is more realistic since MPI tracers are clinically used to evaluate and stratify patients with chronic CAD, and obviously the expected tracer kinetics in such patients vary radically between partial and complete occlusion. The first pig underwent stent insertion and angiography as described in the methods section. An additional angiography, performed at the end of imaging, showed that the stent caused complete occlusion. Rest and stress images with [11C]-4 showed a very severe, non-filling defect (
The second pig, shortly after stent insertion, developed an acute coronary syndrome with inferior wall ST-elevation and eventually development of corresponding q waves in the same leads compatible with myocardial infarction. Since ECG was so conclusive, it was deemed unnecessary to perform angiography after the imaging studies. Once again, images (
Time-activity curves in pig 3 (TACs,
The images of pig 1 (
The images of pig 2 (
Adenosine stress images of pig 3 acquired separately after [13N]NH3 and [11C]-4 injections both show a severe inferolateral defect (
Summary
The addition of a methyl group on the aromatic ring of [11C]-2 to create [11C]-6 changes the partial charge density of the ammonium core. The minor improvement in biodistribution cannot be attributed solely to a change in Log P, since [11C]-6's and [11C]-4's have very similar C Log P (−1.59 and −1.47, respectively); yet [11C]-4's measured parameters are incomparable. When the phenyl in [11C]-2 was replaced with a naphthalene in [11C]-7 (C Log P 0.92), the improvement in biodistribution parameters as well as cardiac uptake was substantial. Cardiac washout, however, did not change and heart/tissue ratios did not improve over time as is the case with [99mTc]-MIBI. When a second phenyl was introduced in [11C]-4, not only did the biodistribution parameters surpass those of the other tracers, but washout from the myocardium became negligible and thus heart/tissue ratios improved over time. It is therefore evident that all three factors affect the biodistribution of the ammonium salt tracers. The most important factor, by far, was found to be the distribution of lipophilicity around the cationic core. Even though [11C]-4's C Log P is lower than that of [11C]-7's, it gave the highest cardiac uptake with negligible washout and improved heart/tissue ratios at later time points.
In light of these results, the distribution of [11C]-4 was evaluated in rats. Similarly to the results obtained with mice, a rapid accumulation of radioactivity was observed in the heart, reaching stable heart SUVs of about 15. Moreover, since radioactivity uptake levels were significantly lower in the lungs, kidneys and the liver, remarkable heart/tissue uptake ratios of 2.4, 3.5 and 3.2 were calculated at 10 min after injection, respectively (
MicroPET studies in rats reinforced the aforementioned biodistribution results. Specifically, rapid and significant myocardial uptake of [11C]-4 was observed within few minutes after injection. When compared to the referenced prevailing MPI PET probe, [13N]NH3, image quality was better following [11C]-4 injection. Two important issues should be addressed in this regard: (i) The % ID/mL values measured in the microPET studies were inconsistent with those obtained from the biosidtribution studies at the same time points. (ii) A moderate, but consistent increase of radioactivity levels in the myocardium was observed in the microPET. The inconsistencies in measured radioactivity concentrations between the two studies could most likely be attributed to the fact that rats were under isoflurane anesthesia during the microPET studies, whereas no anesthesia was applied during the biodistribution experiments. As for the second observation, and since the mild elevation of myocardial radioactivity uptake parallels the continuous reduction of activity levels in the liver, one could not rule out the possible accumulation of radioactive metabolites in the myocardium at later time points. These observations warrant further in-depth investigations.
In addition to the above observations in small animals, we have similarly shown in this study that [11C]-4 accumulates rapidly in the porcine myocardium to high levels, while simultaneously, blood pool and lung activities rapidly clear off. Remarkably, both left ventricular and right ventricular walls are clearly visualized with high target to non-target ratio between the myocardium and heart cavities, respectively. Considerable right ventricular uptake has been shown recently with new fluorine-18 labeled MPI tracers, but is usually less pronounced with traditional PET MPI tracers such as [13N]NH3. In addition, no net washout of [11C]-4 from the myocardium was observed throughout the dynamic acquisition, suggesting that tracer injection away from the camera (e.g., during a treadmill exercise) with PET data collection performed approximately 10 min later is feasible. A point of concern with the [11C]-4 tracer is its significant liver uptake occasionally surpassing that of the myocardium. Liver uptake could potentially interfere with cardiac imaging via radioactivity scatter and through reconstruction artifacts. However, due to better spatial resolution, these problems should not be as significant in PET as they are sometimes in SPECT imaging. Indeed, we encountered no overt imaging problems with our studies that could have been attributed to liver uptake. In addition, inter-species biological variability and also human individual variability of liver uptake can be reasonably anticipated for [11C]-4.
Myocardial images of high quality are certainly desirable for an MPI tracer, yet, above all, its “sine qua non” trademark is identified with the ability to diagnose significant coronary stenosis. Therefore, a good MPI tracer must show the so-called “reversible ischemia” imaging pattern, i.e., a perfusion defect created on stress images that fills-in on rest images. Awareness of this indispensable and essential characteristic of any MPI tracer motivated us to create a novel model for reversible coronary ischemia in swine using a stent wherein its expansion was associated with significant coronary stenosis. Only in the third pig we succeeded in preventing complete obstruction of the artery throughout the experiment, as was evident on coronary angiography performed immediately after stent insertion and additionally at the end of the day. Indeed,
Attempts with stent insertion in pigs 1 and 2 resulted in total coronary artery occlusion as was evident on repeated angiography and by early development of ST-elevation myocardial infarction on ECG monitoring, respectively. The corresponding images show patterns that are compatible with what is considered clinically to be representative of myocardial infarction—a very severe, fixed, inferior wall defect in the case of pig 1 and a severe, fixed, inferior wall defect, with partial inferolateral wall reversibility in the case of pig 2.
The following are the various embodiments of the invention:
A radiolabeled compound of Formula (I):
wherein
each of R1, R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH;
at least one of R1, R2, R3 and R4 is or comprises at least radiolabeled atom or substituent;
each of n and m, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion.
The compound may be one wherein each of R1, R2, R3 and R4, independently of the other, is optionally substituted by one or more substituent selected from C1-C6alkyl, C6-C18aryl, halide (Cl, Br, I, F), nitro, amine, hydroxyl, ether and a chelating agent.
The compound may be one wherein the alkyl group is said C1-C10alkyl or C1-C10aralkyl has 1, 2, or 3 carbon atoms, one of which carbon atoms being substituted by one or more F atoms.
The compound may be one wherein the alkyl group has 1 or 2 carbon atoms, one of which carbon atoms being substituted by one or more F atoms.
The compound may be one wherein the alkyl group is polyethylenglycol (PEG), one of the ethylene carbons may be substituted by an F atom.
The compound may be one wherein at least one of R1, R2 and R3 is a C1-C10alkyl.
The compound may be one wherein at least two of R1, R2 and R3 is a C1-C10alkyl.
The compound may be one wherein each of R1, R2 and R3 is a C1-C8alkyl.
The compound may be one wherein R4 is a radiolabeled C1-C8alkyl.
The compound may be one wherein at least one of R1, R2 and R3 is a C1-C10alkyl and R4 is a C6-C18aryl.
The compound may be one wherein two of R1, R2 and R3 are each, independently, a C1-C10alkyl and the other of R1, R2 and R3 is a C6-C18aryl, and R4 is a C6-C18aryl.
The compound may be one wherein each of R1 and R2, independently, is a C1-C10alkyl and each of R3 and R4, independently, is a C6-C18aryl.
The compound may be one wherein at least one of R1, R2 and R3 is a C6-C18aryl, being optionally substituted.
The compound may be one wherein at least one of R1, R2 and R3 is a C6-C18aryl, being optionally substituted, and R4 is a C1-C10alkyl.
The compound may be one wherein each of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C10alkyl.
The compound may be one wherein at least one of said R1, R2, R3 and R4 is a C1-C10alkyl being different from a methyl, and at least one of said R1, R2, R3 and R4 is a methyl group.
The compound may be one wherein each of R1, R2, R3 and R4 is a methyl group, at least one which being radiolabeled.
The compound is Compound [11C]-1.
The compound may be one wherein two of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C10alkyl and the other two of R1, R2, R3 and R4, independently of the other, is an optionally substituted C6-C18aryl.
The compound may be one wherein three of R1, R2, R3 and R4, independently of each other, is an optionally substituted C1-C10alkyl and the other one of R1, R2, R3 and R4 is an optionally substituted C6-C18aryl.
The compound may be one wherein R1 is a C6-C18aryl.
The compound may be one wherein said aryl being a substituted or unsubstituted phenyl ring.
The compound being a compound of Formula (II):
wherein
each of R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)mOH;
at least one of R2, R3 and R4 is or comprises at least radiolabeled atom or substituent;
R5 is one or two or three or four or five substitutions on the ring, the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C6alkyl, —N(C1-C6alkyl)2), hydroxyl (—OH), C1-C6alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C6alkoxy (—O—C1-C6alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion.
The compound may be one wherein each of R2, R3 and R4 is a C1-C10alkyl, at least one of said R2, R3 and R4 comprising at least one radiolabeled atom or substituent.
The compound may be one wherein each of R2, R3 and R4, independently of the other is a radiolabeled methyl or an ethyl.
The compound may be one wherein each of R2, R3 and R4 is a methyl, one of said methyl groups being radiolabeled.
The compound is Compound [11C]-2.
The compound may be one wherein R5 is one or more substituents, each being independently selected from halide, nitro, amine, hydroxyl, C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH.
The compound may be one wherein R5 is a single substituent at any position of the phenyl ring.
The compound may be one wherein R5 is a single substituent at the ortho position.
The compound may be one wherein R5 is a single substituent at the meta position.
The compound may be one wherein R5 is a single substituent at the para position.
The compound may be one wherein R5 is a halide, an amine, an hydroxyl, or a C1-C10alkyl.
The compound may be one wherein R5 is —NH2.
The compound may be one wherein R5 is —N(C1-C10alkyl)2, wherein each of the two C1-C10alkyl groups is same or different.
The compound may be one wherein the two C1-C10alkyl groups are the same, being selected from methyl and ethyl.
The compound may be one wherein each of the two groups is a methyl.
The compound is Compound [11C]-3.
The compound may be one wherein R5 is a C1-C10alkyl.
The compound may be one wherein the C1-C10alkyl is selected from methyl, ethyl, propyl, iso-propyl and n-butyl.
The compound may be one wherein C1-C10alkyl is a methyl group.
The compound is Compound [11C]-6.
The compound may be one wherein R1 is a C6-C18aryl, said aryl being different from a phenyl.
The compound may be one wherein the C6-C18aryl is naphthyl and the compound is of Formula (III):
wherein the naphthyl moiety may be bonded to the N atom of the quaternary ammonium group via any of the naphthyl carbon atoms, and wherein
each of R2, R3 and R4, independently of each other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)n—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH;
at least one of R2, R3 and R4 is or comprises at least radiolabeled atom or substituent;
R5 is one or two or three or four or five or six or seven substitutions on the naphthyl ring, the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), hydroxyl (—OH), C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C10aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion.
The compound may be one wherein the compound is of the Formula (IV):
The compound may be one wherein R5 is —H.
The compound may be one wherein each of R2, R3 and R4 is a C1-C10alkyl, at least one of said R2, R3 and R4 comprising at least one radiolabeled atom or substituent.
The compound may be one wherein each of R2, R3 and R4, independently of the other is a radiolabeled C1-C10alkyl selected from a methyl, ethyl, propyl, iso-propyl and n-butyl.
The compound may be one wherein each of R2, R3 and R4, independently of the other is a radiolabeled methyl or an ethyl.
The compound may be one wherein each of R2, R3 and R4 is a methyl, one of said methyl groups being radiolabeled.
The compound is Compound [11C]-7.
The compound may be one wherein each of R1 and R2 is a C6-C18aryl.
The compound may be one wherein said aryl is a substituted or unsubstituted phenyl ring.
The compound may be one wherein the compound is of Formula (V):
wherein
each of R3 and R4, independently of the other is selected from C1-C10 alkyl, C2-C10alkenyl, C2-C10alkynyl, C3-C10cycloalkyl, C6-C18aryl, C5-C18heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)6—CH2—OH and C1-C6alkyl-(O—CH2—CH2)m—OH; at least one of R3 and R4 is or comprises at least radiolabeled atom or substituent;
each of R5 and R6, independently of the other, is one or two or three or four or five substitutions on the respective rings (as detailed hereinabove), the substituting group being selected from —H, halide (i.e., Cl, Br, I, F), nitro (—NO2), amine (—NH2, —NH—C1-C10alkyl, —N(C1-C10alkyl)2), hydroxyl (—OH), C1-C10alkyl, C2-C6alkenyl, C2-C6alkynyl, C3-C6cycloalkyl, C1-C10alkoxy (—O—C1-C10alkyl), C6-C18aryl, C5-C10heteroaryl, C1-C10aralkyl, polyethylenglycol, C1-C6alkyl-O—CH2—(CH2—O—CH2)z—CH2—OH and C1-C6alkyl-(O—CH2—CH2)y—OH;
each of n and m, independently of each other is an integer between 2 and 10;
each of z and y, independently of each other is an integer between 2 and 10; and
X is a negatively charged pharmaceutically acceptable ion (anion).
The compound may be one wherein each of R3 and R4 is a C1-C10alkyl, at least one of said R3 and R4 comprising at least one radiolabeled atom or substituent.
The compound may be one wherein at least one of the substituting R5, and/or R6, representing a single substituting or multiple substituting groups is —H or a C1-C10alkyl.
The compound may be one wherein each of R5 and/or R6 is —H, each of R3 and R4, independently of the other is a radiolabeled C1-C10 alkyl selected from a methyl, ethyl, propyl, iso-propyl and n-butyl.
The compound may be one wherein each of R3 and R4, independently of the other is a radiolabeled methyl or an ethyl.
The compound may be one wherein each of R3 and R4 is a methyl, one of said methyl groups being radiolabeled.
The compound is Compound [11C]-4.
The compound may be one wherein at least one of R3 and R4 is not a methyl group.
The compound may be one wherein at least one of R3 and R4 is an ethyl group and the other of R3 and R4 is a methyl group.
The compound may be one wherein one of said of R3 and R4 is optionally substituted.
The compound is Compound [18F]-5.
The compound may be one wherein X is an anion derived from an inorganic acid or from an organic acid.
The compound may be one wherein X is derived from an inorganic acid.
The compound may be one wherein X is selected from sulfate, pyrosulfate, bisulfate, sulfite, bisulfate, nitrate, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, caprylate, isobutyrate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, mandelate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, phthalate, benzenesulfonate, toluenesulfonate, phenylacetate, citrate, lactate, maleate, tartrate, trifluoromethanesulfonate and methanesulfonate.
The compound may be one wherein X is selected from iodide, benzenesulfonate, toluenesulfonate, trifluoromethanesulfonate and methanesulfonate.
The compound may be one wherein X is selected from iodide and trifluoromethanesulfonate.
The compound may be one wherein the radiolabeled atom is selected from 18F, 15O, 13N, and 11C.
The compound may be one wherein the radiolabeled atom is 11C.
The compound may be one wherein the radiolabeled atom is 18F.
The compound may be one wherein the radiolabeled atom is 13N.
The compound may be one wherein the radiolabeled atom is not 18F.
A compound selected from:
A radiopharmaceutical being or comprising a compound of the invention.
The radiopharmaceutical may be in the form of an aqueous solution.
The radiopharmaceutical may be in the form of a formulation optionally further comprising at least one additive.
The radiopharmaceutical may be one wherein said additive is selected from a pH-adjusting agent, a pharmaceutically acceptable stabilizer, an antioxidant, an antimicrobial preservative, an organic solvent and a filler.
The compound or the radiopharmaceutical according to the invention for use in medicine.
The radiopharmaceutical for use in the diagnosis of a disease or disorder.
The radiopharmaceutical for use in risk stratification.
The radiopharmaceutical may be one wherein the disease or disorder is selected from a cardiac disease, renal, neuronal and neoplastic diseases or disorders.
Use of at least one compound according to the invention for the preparation of a composition.
The use may be one wherein said composition is a pharmaceutical composition or a radiopharmaceutical or a diagnostic composition.
The use may be one wherein the diagnostic composition is suitable for use as a biomarker for diagnosis of a disease and disorder in a human and non-human subject using molecular imaging.
The use may be one wherein the molecular imaging is a non-invasive molecular imaging technique.
The use may be one wherein the non-invasive molecular imaging technique is selected from myocardial perfusion imaging (MPI), Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT).
The use may be one wherein the molecular imaging technique is PET.
A diagnostic composition comprising at least one compound according to the invention for use in the diagnosis of a disease or disorder.
The diagnostic composition may be used alongside tracers radiolabeled with a different tracer than the one used in the diagnostic composition.
The diagnostic composition may be one wherein disease or disorder is a cardiac disease, for use in one or more of:
A method for the diagnosis of a disease or disorder, said method comprising administering to a subject suffering from or having predisposition to suffer from or having symptoms which may be associated with the disease or disorder, a diagnostically effective amount of a compound of the invention or a radiopharmaceutical or a diagnostic composition comprising same, and imaging the body or any part of the body of said subject.
The method may be one wherein the disease or disorder is coronary artery disease.
A method of monitoring treatment of a subject, said method comprising administering to a subject having a disease or disorder a compound as defined herein or a radiopharmaceutical, as defined herein, or a diagnostic composition according to the invention and measuring at least one imaging parameter associated with the disease or disorder in the subject using imaging of the body or any part of the body of said subject.
The method may be one wherein the imaging technique is MPI, PET or SPECT.
A method for determining the severity of a disease or disorder, in a subject, the method comprising:
(i) obtaining at least one imaging parameter following the administration of at least one compound according to the invention or at least one radiopharmaceutical or at least one composition comprising same to a subject suffering from the disease or disorder,
(ii) comparing the at least one imaging parameter obtained in (i) with a control reference that correlates with the severity of the disease or disorder, wherein the comparison allowing the determination of the severity of the disease or disorder in the subject.
A method for determining the effectiveness of a therapeutic treatment of a disease or disorder in a subject, said method comprising:
(i) obtaining at least one imaging parameter following the administration, at a first time point, at least one compound according to the invention or at least one radiopharmaceutical or at least one composition comprising same to the subject suffering from the disease or disorder,
(ii) comparing the at least one imaging parameter obtained in (i) with at least one imaging input parameter obtained, following the administration, at a second time point, of at least one radiolabeled compound or at least one radiopharmaceutical or at least one composition to the subject suffering from the disease or disorder, and wherein the comparison allowing the determination of the effectiveness of a therapeutic treatment.
This is a Non-Provisional Application claiming the benefit under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61/348,983, filed on May 27, 2010, the content of each of which is hereby incorporated by reference in its entirety.
| Number | Name | Date | Kind |
|---|---|---|---|
| 4446123 | Woo | May 1984 | A |
| 7112318 | Madar et al. | Sep 2006 | B2 |
| Entry |
|---|
| Fukuda et al. Eur. J. Nucl. Med. (1986) 11:478-483. |
| Zheng et al. Bioorg. Med. Chem. Lett. 13 (2003) 1787-1790. |
| Ilovich, et al., “Novel and Simple Carbon-11-Labeled Ammonium Salts as PET Agents for Myocardial Perfusion Imaging”, Mol Imaging Biol, published online: May 18, 2010. |
| Kim, et al., “In vivo targeting of ERG potassium channels in mice and dogs by a positron-emitting analogue of fluoroclofilium”, Experimental and Molecular Medicine, vol. 37, No. 4, pp. 269-275, (2005). |
| Huang, et al., “In Vivo Stability and Distribution of [131I]Iodomethyl Trimethylammonium Chloride: Concise Communication”, J Nucl Med, vol. 21, No. 7, pp. 679-681, (1980). |
| Burns, et al., “4-[125I] Iodophenyltrimethylammonium Ion, an Iodinated Acetylcholinesterase Inhibitor with Potential as a Myocardial Imaging Agent”, J Nucl Med, vol. 21, No. 9, pp. 875-879, (1980). |
| Madar, et al., “Characterization of Uptake of the New PET Imaging Compound 18F-Fluorobenzyl Triphenyl Phosphonium in Dog Myocardium”, J Nucl Med, vol. 47, pp. 1359-1366, (2006). |
| Yu, et al., “Assessment of 18F-labeled mitochondrial complex I inhibitors as PET myocardial perfusion imaging agents in rats, rabbits, and primates”, Eur J Nucl Med Mol Imaging, vol. 36, pp. 63-72, (2009). |
| Madar, et al., “Characterization of membrane potential-dependent uptake of the novel PET tracer 18F-fluorobenzyl triphenylphosphonium cation”, Eur J Nucl Med Mol Imaging, vol. 34, pp. 2057-2065, (2007). |
| Marshall, et al., “Kinetic Analysis of 18F-Fluorodihydrorotenone as a Deposited Myocardial Flow Tracer: Comparison to 201TI”, J Nucl Med, vol. 45, No. 11, pp. 1950-1959, (2004). |
| Crouzel, et al., “Recommendations for a Practical Production of [11C]methyl Iodide”, Appl. Radiat. Isot., Int. J. Radiat. Appl. Instrum. Part A, vol. 38, No. 8, pp. 601-603, (1987). |
| Russell, et al., “Nuclear Cardiology: Present and Future”, Curr Probl Cardiol, vol. 31, pp. 557-629, (2006). |
| Underwood, et al., “Myocardial perfusion scintigraphy and cost effectiveness of diagnosis and management of coronary heart disease”, Heart, vol. 90 (Suppl V), pp. v34-v36, (2004). |
| Hachamovitch, et al., “Value of Stress Myocardial Perfusion Single Photon Emission Computed Tomography in Patients With Normal Resting Electrocardiograms: An Evaluation of Incremental Prognostic Value and Cost-Effectiveness”, Circulation, vol. 105, pp. 823-829, (2002). |
| Marcassa, et al., “Clinical value, cost-effectiveness, and safety of myocardial perfusion scintigraphy: a position statement”, European Heart Journal, vol. 29, pp. 557-563, (2008). |
| Beller, “First Annual Mario S. Verani, MD, Memorial Lecture: Clinical value of myocardial perfusion imaging in coronary artery disease”, J Nucl Cardiol, vol. 10, pp. 529-542, (2003). |
| Aerts, et al., “Metabolism of no-carrier-added 2-[18F]fluoro-L-tyrosine in rats”, BMC Medical Physics, vol. 8, No. 4, pp. 1-7, (2008). |
| Mazière, et al., “In Vivo Characterization Of Myocardium Muscarinic Receptors by Positron Emission Tomography”, Life Sciences, vol. 29, pp. 2391-2397, (1981). |
| Wang, et al., “Facile synthesis of new carbon-11 labeled conformationally restricted rivastigmine analogues as potential PET agents for imaging AChE and BChE enzymes”, Applied Radiation and Isotopes, vol. 66, pp. 506-512, (2008). |
| Schillaci, et al., “18F-choline PET/CT physiological distribution and pitfalls in image interpretation: experience in 80 patients with prostate cancer”, Nucl Med Commun, vol. 31, p. 39-45, (2010). |
| Kil, et al., “Synthesis and positron emission tomography studies of carbon-11-labeled imatinib (Gleevec)”, Nuclear Medicine and Biology, vol. 34, pp. 153-163, (2007). |
| Studenov, et al., “Synthesis and properties of 18F-labeled potential myocardial blood flow tracers”, Nuclear Medicine and Biology, vol. 28, pp. 683-693, (2001). |
| Apolo, et al., “Novel Tracers and Their Development for the Imaging of Metastatic Prostate Cancer”, J Nucl Med, vol. 49, pp. 2031-2041, (2008). |
| Degrado, et al., “Synthesis and Evaluation of 18F-labeled Choline as an Oncologic Tracer for Positron Emission Tomography: Initial Findings in Prostate Cancer”, Cancer Res, vol. 61, pp. 110-117, (2000). |
| Degrado, et al., “Synthesis and Evaluation of 18F-Labeled Choline Analogs as Oncologic PET Tracers”, J Nucl Med, vol. 42, pp. 1805-1814, (2001). |
| Herrero, et al., “Comparison of 1-11C-Glucose and 18F-FDG for Quantifying Myocardial Glucose Use with PET”, The Journal of Nuclear Medicine, vol. 43, No. 11, pp. 1530-1541, (2002). |
| Jonson, et al., “Investigations into tumor accumulation and peroxisome proliferator activated receptor binding by F-18 and C-11 fatty acids”, Nuclear Medicine and Biology, vol. 29, pp. 211-216, (2002). |
| Torstenson, et al., “A Comparison of 11C-Labeled L-DOPA and L-Fluorodopa as Positron Emission Tomography Tracers for the Presynaptic Dopaminergic System”, Journal of Cerebral Blood Flow and Metabolism, vol. 19, pp. 1142-1149, (1999). |
| Volkow, et al., “Mechanism of action of methylphenidate: Insights from PET imaging studies”, Journal of Attention Disorders, vol. 6, Supplement Jan. 2002, pp. S-31-S-43, (2002). |
| Number | Date | Country | |
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| 20110293519 A1 | Dec 2011 | US |
| Number | Date | Country | |
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| 61348983 | May 2010 | US |
