Molecular Phenotyping and Genotyping Core

Information

  • Research Project
  • 10160881
  • ApplicationId
    10160881
  • Core Project Number
    P30DK036836
  • Full Project Number
    5P30DK036836-35
  • Serial Number
    036836
  • FOA Number
    RFA-DK-16-020
  • Sub Project Id
    7096
  • Project Start Date
    2/15/1997 - 27 years ago
  • Project End Date
    3/31/2022 - 2 years ago
  • Program Officer Name
  • Budget Start Date
    4/1/2021 - 3 years ago
  • Budget End Date
    3/31/2022 - 2 years ago
  • Fiscal Year
    2021
  • Support Year
    35
  • Suffix
  • Award Notice Date
    3/29/2021 - 3 years ago

Molecular Phenotyping and Genotyping Core

CORE 6 - MOLECULAR PHENOTYPING AND GENOTYPING : ABSTRACT The objective of the Molecular Phenotyping and Genotyping Core is to support Joslin and external investigators in the study of molecular mechanisms of disease by providing equipment, expertise, and services in molecular phenotyping, including nucleic acid sequence analyses, gene expression, and other ?-omics? analyses, which would be too specialized or costly for individual laboratories to perform independently. Formerly known as the Advanced Genomics and Genetics Core, the Molecular Phenotyping Core is newly- named in recognition of the expansion of its analytical capacities to reflect not only genetic and genomic analyses but also metabolic phenotyping. The new Core will continue to provide the services previously offered, including: 1. DNA extraction from blood; 2. Curation of and maintenance of access to DNA collections from the Core's repository; 3. SNP genotyping; 4. Bioanalyzer analysis of DNA/RNA quality and quantity; 5. DNA shearing; 6. Preparation of next-generation sequencing libraries; 7. Facilitated access to external next- generation sequencing facilities; 8. Gene expression using microarrays (Affymetrix), including cRNA/cDNA preparation and hybridization; 9. Access to real-time quantitative PCR; 10. Assistance with study design and data analysis in collaboration with the Boston University-Joslin Regional Computational (BUJRC) Core;11. Educational services to facilitate the understanding and the use of next-gen and other -omics methodologies by the Joslin community. New services to enhance capacity for the study of molecular mechanisms of disease will include: 1. Genetic characterization for clinical studies (a next-gen sequencing assay developed by the core to simultaneously screen all known monogenic diabetes genes for mutations and to type all known SNPs associated with T1DM, T2DM, or diabetic complications) 2. Bioenergetics and metabolic phenotyping (analysis of metabolic flux using the Seahorse XF24 and XF96 instruments and facilitated access to metabolomics platforms). 3. Plasma proteomics (facilitated access to the Somalogic proteomic platform at BIDMC). 4. Digital genomics (through a new digital PCR platform that is being purchased by the Joslin for the digital detection of RNA, DNA, and noncoding RNA species). Training will be an important component of introduction of these services, so as to promote utilization of these approaches by investigators and trainees alike. Together, these new initiatives will facilitate the ongoing transition of Joslin investigators to state-of-the art phenotyping and genotyping that that are instrumental for both basic, translational, and clinical research studies.

IC Name
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
  • Activity
    P30
  • Administering IC
    DK
  • Application Type
    5
  • Direct Cost Amount
    151658
  • Indirect Cost Amount
    104644
  • Total Cost
  • Sub Project Total Cost
    256302
  • ARRA Funded
    False
  • CFDA Code
  • Ed Inst. Type
  • Funding ICs
    NIDDK:256302\
  • Funding Mechanism
    RESEARCH CENTERS
  • Study Section
    ZDK1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    JOSLIN DIABETES CENTER
  • Organization Department
  • Organization DUNS
    071723084
  • Organization City
    BOSTON
  • Organization State
    MA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    022155306
  • Organization District
    UNITED STATES