Claims
- 1. A method for neutralizing a polypeptide, comprising contacting a polypeptide having an amino acid sequence of SEQ ID NO:1, where Xaa represents isoleucine or threonine, with a monoclonal antibody obtained from antibody-producing mammalian cells that binds to the polypeptide having the amino acid sequence of SEQ ID NO:1.
- 2. A method for detecting the presence of a polypeptide capable of inducing interferon-γ production in immunocompetent cells and comprising an amino acid sequence of SEQ ID NO:1, where Xaa represents isoleucine or threonine, comprising:
contacting a monoclonal antibody with a sample, wherein said monoclonal antibody is prepared by using either a polypeptide comprising an amino acid sequence of SEQ ID NO:1, where Xaa represents isoleucine or threonine, and having a molecular weight of 18,500±3,000 daltons on SDS-PAGE and a pI of 4.9±1.0 on chromatofocusing, or an antigenic fragment of said polypeptide as an antigen, and said monoclonal antibody binds to said polypeptide or said fragment thereof; and detecting the occurrence of the immunoreaction between said monoclonal antibody and said polypeptide in the sample.
- 3. The method of claim 2, wherein the monoclonal antibody is labelled with a detectable label selected from the group consisting of radioisotopes, enzymes and fluorescent labels.
- 4. The method of claim 2, further comprising the step of determining the level of immunoreaction detected between said monoclonal antibody and said polypeptide in the sample.
Priority Claims (1)
Number |
Date |
Country |
Kind |
58240/1995 |
Feb 1995 |
JP |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This is a division of application Ser. No. 09/711,899, filed Nov. 15, 2000, which is a division of application Ser. No. 08/558,818, filed Nov. 15, 1995, the entire contents of both applications are incorporated herein by reference.
Divisions (2)
|
Number |
Date |
Country |
Parent |
09711899 |
Nov 2000 |
US |
Child |
10327069 |
Dec 2002 |
US |
Parent |
08558818 |
Nov 1995 |
US |
Child |
09711899 |
Nov 2000 |
US |