This disclosure relates to the formation of micro-tissues and of application of the micro-tissues to organisms.
Administration of cells to patients has become a promising therapy for many diseases. However, cells delivered in single cell suspension often die quickly after being injected into the site for repair and it is also unknown if single cells can adequately repair damaged or failing organs and tissues that are composed of millions of cells. Current methods for cell injection therapy involve using enzymatic release of cells into a single cell suspension which causes changes in their cellular structure and phenotype. Additionally, injected single cells often die after injection from a combination of stress induced from the physical injection process and lack of attachment to the desired tissue.
The systems and processes described in this document allow for the formation of small intact cell patches (e.g., micro-tissues) and application of the micro-tissues to a body of a patient. The thermal release of these micro-tissues from a substrate (e.g., an extracellular matrix protein scaffold (ECM)) on which the cell patches are cultured allows for the cells to maintain their structure and phenotype, which allows the cells to repair damaged tissue after delivery. The extracellular matrix is configured to surround the cultured cell patch (e.g. a monolayer of cells) to protect the cell patch from physical stress of a delivery process to the body. The ECM also provides an attachment site that facilitates attachment of the cell patch to a desired target tissue (e.g., for tissue repair).
The ECM can include encapsulation materials that replicate a micro-environment desired for the cell patch in vivo. For example, the ECM is configured to be similar in density, structure, and/or composition to the native ECM these cells are surrounded by in vivo. The ECM provides a unique microenvironment that more closely matches that found in vivo and thus improves the ability to modulate cell behavior.
The cell patch can include a cell monolayer. The monolayer is formed to include cell expressions for cell-cell tight junctions in the cell patch. A relatively larger substrate and longer culture time (e.g., −24 hours) enable tight junctions to form. These junctions generally appear in vivo and enable the cell patch to be more readily accepted into tissue when administered.
The shrink-wrapped monolayers provide one or more of the following advantages. Biological features of the cells are maintained for in vivo administration, in contrast to enzyme-based approaches to releasing cells from a scaffold. The cells maintain their phenotype (e.g., tight junctions) in the monolayer. The cells form patches of empithelium and/or build a cytoskeleton that can be maintained for cell administration. For example, when introduced in vivo in a variety of contexts (e.g., in the heart, cornea, or for lung repair), cells integrate into tissue easily, as subsequently described. Specifically, the cell-cell junctions are maintained.
The cell patches can be used for treating diseases, as subsequently described. For example, cornea repair can be performed using cell monolayers. Cardiac repair can be performed. In some implementations, lung repair can be performed.
As subsequently described, monolayers provide other advantages with respect to single cell approaches. For example, the endothelium and/or epithelium are barriers for cell administration. Because the cells monolayers being administered are form cell patches, the endothelium allows the patches to integrate. In some implementations, the cell patch attaches as a dome of cells, and flattens and integrates over time with the endothelium. Additionally, monolayer cells migrate out from patch to rest of endothelium, which does not occur when individual cells are applied. Individual cells are less likely to attach. The monolayers thus result in increased survival with respect to single cells. The cell junctions can affect cell signaling to promote cell adhesion in this way, which increases viability, as the cell phenotype is maintained.
In some implementations, the cells additional materials are added to the extracellular matrix to facilitate patterning. For example, antibodies can be added to deliver to a specific location (e.g., a targeted delivery). In some implementations, growth factors can be added to the matrix that is shrink wrapped (e.g., pbGF for muscle cells). This can enhance, for example, vascular ingrowth which is needed for muscle cells. In some implementations, this approach includes labeling for validation. For example, cell tracker (e.g., cytoplasmic dyes) can be used.
In some implementations, different shapes of scaffolds are possible. In addition to squares, tubes can be generated to generate tubules. This can facilitate growth of fragments of blood vessels and/or chains of endothelial cells. In some implementations, it is possible to mix different kinds of shrinkwrapped cells.
Various disease treatments are possible using the shrinkwrapped cells. For example, in some embodiments, described herein includes injecting cells into patients that suffer from corneal blindness due to low cell density. In some embodiments, described herein includes the injection of micro-monolayers that can increase the cell density without the need to remove the existing endothelium.
In some embodiments, described herein includes the integration of corneal endothelial cell micro-monolayers that can be injected through a small gauge need to help alleviate density driven corneal blindness.
In some embodiments, described herein includes an array of cell assemblies that can be created by an extracellular matrix shrink wrapped cells (SHELL) technique, wherein upon thermal release, the cell assemblies can maintain their cell-cell junctions and can contract into a size small enough to be injected into tissues through a needle.
In some embodiments, described herein includes an in vivo injection process that involves maintaining an injected eye down after injection to allow for integration of the cell assembly into the corneal endothelium. In some embodiments, described herein includes the synthesis of an extracellular matrix SHELL scaffold that can help maintain the cells in a monolayer and can maintain the cytoskeleton of the cell in the monolayer, which together with their cell-cell junctions can improve integration.
In an aspect, a process for micro-tissue encapsulation of cells includes coating a tissue scaffold stamp with an extracellular matrix compound. The process includes depositing the tissue scaffold stamp onto a thermoresponsive substrate. The process includes seeding the tissue scaffold stamp with a cell culture. The process includes incubating the cell culture on the tissue scaffold stamp at a temperature that is specified, wherein the cell culture forms a cell patch that is attached to the extracellular matrix compound. The process includes forming, by the cell patch, a monolayer on the tissue scaffold stamp in which borders of the monolayer maintain expressions for cell-cell junctions. The process includes removing the thermoresponsive substrate. The process u) includes removing the tissue scaffold stamp from the cell patch to form a micro-tissue structure around the cell patch. The process includes folding the micro-tissue structure by suspending the micro-tissue in the solvent. The process includes collecting the folded micro-tissue structure from the solvent. The process includes administering the folded micro-tissue structure to an organism.
In some implementations, the process includes forming the tissue scaffold into a tube configuration. In some implementations, the process includes forming a cell patch comprising a tube geometry based on the tube configuration of the tissue scaffold.
In some implementations, the cell patch comprises a fragment of a blood vessel. In some implementations, the process includes adding antibodies to the cell patch. In some implementations, administering the micro-tissue structure to an organism comprises injecting the micro-tissue structure. In some implementations, the cell patch comprises corneal endothelial cells. The process includes introducing the cell patch to a cornea and ensuring a contact of the cell patch with the cornea using gravity.
In some implementations, a size of the micro-tissue structure is proportional to a size of the tissue scaffold stamp, and wherein the size of the micro-tissue structure is a fraction of a diameter of an injecting apparatus. In some implementations, the tissue scaffold stamp comprises an organosilicon compound. In some implementations, the organosilicon compound comprises Polydimethylsiloxane. In some implementations, the extracellular matrix compound comprises a protein comprising one or more of collagen IV, laminin, a fibroblast growth factor protein, and a vascular endothelial growth factor protein.
In some implementations, depositing the tissue scaffold stamp comprises printing the tissue scaffold stamp onto the thermoresponsive substrate. In some implementations, the thermoresponsive substrate comprises a PIPAAm polymer. In some implementations, the tissue scaffold stamp forms a regular geometry. In some implementations, the tissue scaffold stamp comprises a surface dimension of less than or approximately equal to 250 μm2. In some implementations, the cell patch comprises between 10 and 100 cells.
In a general aspect, a system includes a cell patch comprising a cell monolayer that maintains expressions for cell-cell junctions and cytoskeletons for cells that are in the cell patch. The system includes a micro-tissue structure folded around the cell patch, the micro-tissue structure comprising an extracellular matrix configured to provide a physical barrier between the cell patch and an external environment.
In some implementations, the extracellular matrix comprises a protein comprising one or more of collagen IV, laminin, a fibroblast growth factor protein, and a vascular endothelial growth factor protein. In some implementations, the micro-tissue structure forms a tube configuration. In some implementations, the monolayer comprises between and 100 cells. In some implementations, the cell patch comprises muscle tissue. In some implementations, a growth factor is added to the extracellular matrix to promote vascular ingrowth of the muscle tissue.
The details of one or more embodiments of these systems and methods are set forth in the accompanying drawings and the description to be presented. Other features, objects, and advantages of these systems and methods will be apparent from the description and drawings, and from the claims.
The extracellular matrix (ECM) described herein includes an array of geometrical shapes that will fold upon release. For example, the ECM can form one or more patterned geometries that have micrometer dimensions for length and width and nanometer thickness. In some examples, the geometrical shapes in the array include extracellular matrix proteins that can be used to culture cells that allow for the formation of 2D micro-tissues, specifically cell monolayers. These micro-tissues can then be released from a substrate upon which they are formed. In some implementations, the micro-tissues are thermally released. Upon release, these micro-tissues spontaneously fold up such that the ECM forms an outer layer around the cultured cells, and the cultured cells retain their micro-tissue structure and phenotype. The folded micro-tissues can then be administered, for example by injection through a needle, to repair or replace tissues. The micro-tissues have in vivo and in vitro applications. For example, the micro-tissues support the formation of corneal endothelial cell micro-tissues that can be injected through a small gauge needle.
The processes and systems described herein include encapsulation of micro-monolayer cell patches (e.g., μMonolayers) in a thin layer of ECM protein that allows cells to maintain high viability, cell-cell junctions, and cytoskeletal structure post-injection, while also providing the cells with cell-ECM interact ions that can promote cell adhesion. In vitro results confirm that the CE cells formed a monolayer on the engineered ECM substrates and within 24 hours had established tight junctions and formed an organized F-actin cytoskeletal structure, which was retained through the thermal release and injection process. When compared to enzymatically-released single cells, injected shrink-wrapped μMonolayers significantly increase CE monolayer cell density, as subsequently described.
Additionally, shrink-wrapped μMonolayers attached to both in vitro mono layers and ex vivo corneas within 3 hours, indicating that the described encapsulated processes could be applied using the protocols that are currently in use in clinical trials for single cell CE inject ion. For example, in vivo rabbit studies utilizing healthy rabbit eyes showed high numbers of shrink-wrapped μMonolayers integrated into the healthy CE. Generally, cells within a young healthy rabbit CE are contact-inhibited and are at an extremely high density. Therefore, if the shrink-wrapped μMonolayers are able to integrate within such a tissue, integration into damaged or diseased CEs with a much lower cell density occur at much higher rates.
Generally, the ECM includes a fibrillar network of proteins, glycosaminoglycans and other biomolecules. The ECM forms a scaffold around cells that provides, for example, structural support, growth factor sequestration, a network for adhesion and mechanical signaling, and a host of other functions. The ECM can function as an environment, or niche, that is suitable for the functioning of the cultured cells of the micro-tissues. For example, the adult stem cell niche includes a unique ECM protein structure, composition, support cell population and set of soluble and insoluble signaling molecules that help maintain the multipotent state of the stem cells. The ECM is an artificially produced protein matrix, rather than a naturally-occurring ECM. The selection of ECM proteins is chosen based on the cell culture being produced, as described in further detail below.
In some examples, 2D culture cells are typically grown on rigid tissue culture treated polystyrene (TCPS) that is pre-coated with an ECM protein or coated with ECM proteins that are included in a serum supplemented into the media. While such ECM proteins enable adhesion of cells to the TCPS and subsequent proliferation, many primary cell types can only be passaged a limited number of times before becoming senescent of changing phenotype, such as undergoing epithelial to mesenchymal transition (EMT). Culture in 3D using synthetic and/or natural hydrogels can address some of these limitations by altering the chemo-mechanical environment to better replicate in vivo conditions and have been effective for culturing a wide range of cell types. However, these hydrogels are typically isotropic in structure, do not recreate ECM dense structures such as basement membranes and have compositions (e.g., collagen, fibrin, matrigel, PEG) that typically differ from that of the complex in vivo environment. Further, passaging these cells, whether in 2D or 3D, often requires using enzymes and calcium chelators that disrupt cell-matrix and cell-cell adhesion to produce a single cell suspension. When re-seeded the cells must expend energy to reestablish cell matrix and cell-cell adhesions in the new environment into which they are placed. The ECM is configured to mimic a cell micro-environment that is found in vivo by (i) encapsulating cells in a defined ECM that better mimics the native ECM structure and (ii) minimally disrupting cell-matrix and cell-cell adhesions.
ECM nano-scaffolds are formed that can be used to at least partially encapsulate cells in order to modulate the chemo-mechanical microenvironment. Using an adaptation of surface-initiated assembly (SIA), well-defined nano-scaffolds of assembled ECM proteins are formed into free standing structures. By adhering cells prior to the release of these ECM nano-scaffolds, the cells are encapsulated (e.g., shrink-wrapped), in a layer of assembled protein matrix. In some implementations the ECM nano-scaffolds are engineered at the size scale of the cell, ˜75 μm in lateral dimensions and ˜50 nm thick. In some implementations, the SIA approach can be used to encapsulate a variety of cell types in defined ECM including one or more of fibronectin (FN), laminin (LAM), fibrinogen (FIB) and collagen type IV (Col IV), representing the major protein composition of the native pericellular matrix. The long-term goal is that these ECM nano-scaffolds and the encapsulation process will enhance therapeutic cell delivery by supporting survival and functional integration of cells in an otherwise diseased matrix environment, such as that found in infarcted myocardium.
The ECM nano-scaffolds can be used with any adherent cell type and could even be extended to non-adherent cells if antibodies for cell surface markers are mixed in the ECM protein solution before incubating on the PDMS stamp. For example, cell types can include hepatocytes, which includes an adherent cell type. For example, cell types can include killer t cell, which are a non-adherent cell-type, and are combined with a cell surface marker antibody in the ECM. These cell types can be used with collagen I, collagen IV, fibronectin, laminin, vitronectin, and any ECM protein that can be micro-contact printed.
The process 100 can be performed for each of the following embodiments.
ECM scaffold fabrication The ECM scaffolds were fabricated via previously described surface-initiated assembly with minor modifications (e.g., shrink wrap paper). Briefly, 1 centimeter (cm) by 1 cm PDMS stamps designed to have 200 μm by 200 μm square features were fabricated via standard soft lithography techniques. The stamps were sonicated in 50% ethanol for 60 minutes, dried under a stream of nitrogen and incubated for 60 minutes with a 50:50 mixture of 50 μg/mL collagen IV (COL4) and 50 μg/mL laminin (LAM), as shown in step 110. Either 50% AlexaFluor 488 labeled COL4 or 50% AlexaFluor 633 labeled LAM was used to visualize the protein. Following incubation the stamps were rinsed in sterile water, dried under a stream of nitrogen and brought into conformal contact with poly(N-isopropylacrylamide) (PIPAAm) (2% high molecular weight, Scientific Polymers) coated 25 mm glass coverslips for 30 minutes to ensure transfer of the squares, as shown in step 120. ECM square micro-contact printed on PDMS coverslips were used as controls. Upon stamp removal, laser scanning confocal microscopy was used to determine the quality of the transferred ECM squares (Nikon AZ I 00).
Bovine corneal endothelial cells (BCECs) were isolated and cultured as previously described (ref EBM and expansion paper). Briefly, corneas were excised from the whole globe (Pel Freez), incubated endothelial side up in a ceramic 12 well spot plate with 400 μL of TrypLE Express for 20 minutes. The cells were then gently scraped from the cornea using a rubber spatula, centrifuged at 1500 RPM for 5 minutes, re-suspended in 5 mL of culture media (low glucose DMEM with 10% FBS, 1% Pen/Strep/AmphB and 0.5% gentamicin, designated at P0 and cultured in a 50 kPa PDMS coated T-25 flask that was pre coated with COL4. Fifty whole eyes were received at a time and were used to seed 5 T-25 flasks. Cells were cultured until confluence and split 1:3 until they were used once confluent at P2.
Shrink-Wrapping BCEC uMonolayers in ECM Scaffolds
Patterned coverslips (25 mm) were secured with vacuum grease in the bottom of 35 mm petri dishes which were placed on dry block set to 52° C. This resulted in the coverslips reaching (within 30 min) and holding at 40° C. Bovine CECs were released from the culture flask with TrypLE Express, centrifuged and re-suspended at a density of 150,000 cells/mL in 15 mL centrifuge tubes. The tubes were placed in a dry block set at 45° C. for approximately 5 minutes, or until the cell solution just reached 40° C. and 2 mL of cell suspension was added to each 35 mm dish before it was immediately placed in an incubator (37° C., 5% CO2). Cells were cultured on the squares for 24 hours to allow them to form small monolayers on the 200 μm squares. After 24 hours, samples were removed from the incubator, rinsed twice in 37° C. media, 2 mL of fresh warm media was added, and the sample was allowed to cool to room temperature. Once the temperature decreased <32° C. the PIPAAm dissolved and released the scaffolds+μMonolayers. The release process was recorded using a Photometrics CoolSnap camera. Following release the scaffolds+μMonolayers were collected via centrifugation at 1500 rpm for 5 minutes before use in further experiments. CECs seeded on to PDMS coverslips were used as a control.
Immunostaining of shrink-wrapped BCECs: Shrink-wrapped μMonolayers re-suspended in PBS containing Ca2 and Mg2 (PBS++) were injected through a small gauge needle onto a glass coverslip and allowed to settle for about 15 minutes before fixation for 15 minutes in 4% paraformaldehyde (PBS++). Samples were gently washed 2×'s with PBS++ and incubated with 1:100 dilution of DAPI, 1: I 00 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488. Samples were rinsed 2 times for 5 minutes with PBS++ and incubated with 1:100 dilution of AlexaFluor 555 goat anti-mouse secondary antibody for about 2 hours. Samples were rinsed 2 times for 5 minutes with PBS++, mounted on glass slides with Pro-Long Gold Antifade (Life Technologies) and then imaged on a Zeiss LSM 700 confocal microscope.
Viability of Shrink-Wrapped BCECs Post-Injection
After centrifugation, shrink-wrapped μMonolayers or TrypLE Express released single cells were re-suspended in 200 μL of growth media, drawn up into a 280 needle, injected into a petri dish and incubated with 2 μM calcein AM and 4 μM EthD-1 (Live/Dead Viability/Cytoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37° C. After 30 minutes, samples were imaged on a Zeiss LSM 700 confocal; 5 images per sample and 3 samples per type were used. The number of live and dead cells was counted manually. The number of live cells was divided by the number of dead cells to determine the percent viability of both the ECM scaffold wrapped cells and enzymatically released cells.
Seeding of Shrink-Wrapped and Single BCECs on Stromal Mimics
Self-compressed collagen I films were prepared as previously described. Briefly, a 6 mg/mL collagen I gel solution was prepared per manufacturer's instructions and pipetted into 9 mm diameter silicone ring molds on top of glass coverslips. The gels were placed into a humid incubator (37° C., 5% CO2) for 3 hours to compress under their own weight. The gels were then dried completely in a biohood followed by rehydration in PBS, forming a thin collagen I stromal mimic. Shrink-w rapped BCEC μMonolayers were seeded onto the films at a 1:1 ratio of stamped coverslip to collagen I film. As a control, BCECs that were cultured in the flasks and enzymatically released using TrypLE Express into a single cell suspension were seeded onto collagen I films. The number of control cells seeded was equal to the number of cells that would be seeded from one stamped ECM scaffold sample if all squares had full monolayers on them, as a best case scenario. The average number of cells that occupied the 200 μm square was 30 cells; so cells×1600 squares per stamp, meaning approximately 48,000 cells per sample. Therefore, 50,000 cells per sample were seeded for the controls. At 6 and 24 hours, samples were removed from culture and fixed and stained for the nucleus, ZO-1 (tight junction protein) and F-actin.
Briefly, samples were rinsed twice in PBS++, fixed in 4% parafolmaldehyde (in PBS++) with 0.05% Triton-X 100 for 15 minutes. Samples were rinsed twice for 5 minutes with PBS++ and incubated with 5 drops of NucBlue (Life Technologies) for 10 minutes. Samples were rinsed once with PBS++ and incubated with 1:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488 or 633 phalloidin for 2 hours. Samples were rinsed 3 times for 5 minutes with PBS++ and incubated with 1:100 dilution of AlexaFluor 555 goat anti-mouse secondary antibody for 2 hours. Samples were rinsed 3 times for 5 minutes with PBS++, mounted on glass slides using Pro-Long Gold Anti-fade and imaged on a Zeiss LSM 700 confocal microscope.
In Vitro Integration of Shrink-Wrapped Vs Single Bovine CECs
To mimic a low-density aging CE, 25,000 PS bovine cells were seeding onto the collagen I stromal mimics as described above, until confluent to form the “aged” monolayers. Shrink-wrapped μMonolayers and single BCECs were prepared as previously described, labeled with CellTracker Green (Life Technologies) for 30 minutes, centrifuged, diluted to the equivalent of 50,000 cells/sample and injected on to the “aged” monolayers. “Aged” monolayers with no cells injected on top served as controls. Samples were fixed at stained at days 3, 7 and 14 as described above. A Zeiss LSM700 confocal was used to image 10 random spots on each sample and the cell density was manually counted (e.g., cell nuclei). The number of nuclei was divided by the image area to obtain the cells/mm 2 per image. The cell density for each sample was determined by averaging the cell densities of each image and the average cell density of each sample type was determined by averaging the cell density of the 3 or 4 samples. To examine the outgrowth of the shrink-wrapped μMonolayers over time, confocal images centered around and individual shrink-wrapped μMonolayer (day 3 n=33, day 7 n=37, day 14 n=40) were collected and the CellTracker channel was converted in to a binary black and white image. The binary images for each sample type were then converted in to a Z-stack and analyzed via the Heat Map for Z-stacks plugin (relative without log 10) to determine the average pixel density of CellTracker.
Live Imaging of In Vitro Integration of Shrink-Wrapped Bovine CECs
For live imaging, the “aged” monolayer on the collagen I stromal mimic, was first incubated for 30 minutes with CellTracker Orange to differentiate between the existing monolayer and injected cells, which were labeled with CellTracker Green as described above. HEPES buffered Opti-MEM I Reduced Serum Media (Life Technologies) with 10% FBS, 1% Pen/Strep was added to the monolayer and Shrink-wrapped μMonolayers that were prepared as described above were injected through a 30G needle on top of the sample. The sample was placed on the Zeiss LSM700 confocal equipped with a temperature chamber set to 37° C. for 30 minutes to allow for the cells to settle. Using the Definite Focus system, a time lapse series of one z-stack was obtained every hour for 48 hours.
Rabbit CEC Isolation, Culture and Shrink-Wrapping
Whole rabbit eyes were received on ice from Pel Freez Biologicals. Corneas were excised from the whole globe, the CE and Descemet's Membrane were manually stripped with forceps and incubated in Dispase (1U/mL, Stem Cell Technologies) for 1.5 hours at 37° C. to detach the rabbit CE cells (RCECs) from the Descemet's Membrane. The RCECs were then gently pipetted up and down, diluted in culture media (DMEM/F12, 10% FBS, 0.5% Pen/strep), centrifuged at 1500 RPM for 5 minutes, re-suspended in 10 mL of culture media, designated at P0 and cultured COL4 coated T-25 flasks with the equivalent of 15-25 eyes per flask depending on cell yield. RCECs were cultured until confluence and split 1:2 and used in all experiments once confluent at P1 or P2. RCEC μMonolayers were shrink-wrapped as described above with the following modifications: ECM scaffolds (using the same 1 cm×1 cm PDMS stamps) were stamped on to 18 mm glass coverslips to avoid having excess seeding area and reduce the number of cells that need to be seeded per sample to still achieve full coverage with −50,000 cells/sample once confluent. Coverslips were secured via vacuum grease to the bottom of Nunc IVF center well dishes (20 mm diameter inner well), cells were re-suspended at 150,000 cells/mL and 1 mL of RCECs was seeded per sample.
Ex Vivo Integration of Shrink-Wrapped Rabbit CECs
Three whole rabbit eyes were placed cornea up in a 12-well plate. Shrink-wrapped RCEC μMonolayers were prepared as described above. Two samples of μMonolayers per ex vivo eye were prepared and re-suspended in 100 μL of DMEM/F12. A 30-G insulin syringe was used to draw up the full I 00 μL, the needle was inserted in to the center of the cornea until it was visible in the anterior chamber and 504, of the suspension was injected. This resulted in the equivalent of 50,000 cells injected into the anterior chamber. The needle was held in place for a few seconds to ensure the media and cells did not come back out of the injection site. The injection was viewed under a stereomicroscope and the pink color of the media filling the anterior chamber was visible, indicating successful injection. The eyes were flipped and incubated cornea down for 3 hours at 37° C., 5% CO2 in a humidified incubator. After 3 hours, the whole eye was placed in 2% paraformaldehyde (PBS++) at 4° C. for 24 hours. After 24 hours the eye was rinsed in PBS and the cornea was excised and rinsed 3×'s for 5 minutes. The cornea was then incubated CE facing down on 1 mL of PBS++ containing 2 drops of NucBlue (Life Technologies), 2:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488 Phalloidin (Life Technologies) for 2 hours at room temperature. Corneas were then rinsed 3×'s for 5 minutes in PBS followed by 2 hour incubation on 1 mL PBS++ with 2:100 dilution of AlexaFluor 555 goat anti-mouse secondary antibody for 2 hours and stored in PBS before imaging on the Zeiss LSM700 confocal.
In Vivo Injection and Integration of Shrink-Wrapped CECs
Shrink-wrapped RCEC μMonolayers were prepared as described above with one minor modification: cells were labeled with Vybrant DiO 1 day prior to seeding on to the ECM nano-scaffold s by incubating cells in 1 mL of media with 5 μL of Vybrant DiO for 30 minutes followed by 3 ten minute rinses with fresh media. An excess number of μMonolayer samples were prepared (16) in order to ensure there was enough volume for injection. The Shrink-wrapped μMonolayers were released and were re-suspended in one tube 1.5 mL micro-centrifuge tube in a total of 300 μL. Three female New Zealand white rabbits with healthy intact CEs weighing approximately 2.5 kg were used for this study. Rabbits were anesthetized with Ketamine (40 mg/kg) and Xylazine (4 mg/kg) followed by isofluorene to keep rabbits under sedation for 3 hours. Rabbit #1 was injected in right eye (OD) with 504, (˜100,000 cells). Rabbit #2 was initially injected with 1004, in to the right eye, however most of the volume came back out of the cornea, so an additional 504, was injected in to a second location for 300,000 cell injection if all cells had successfully stayed within the anterior chamber. Rabbit #3 had a smaller than usual right eye so the left eye was injected with 1004, or approximately 200,000 cells. Immediately after injection, each rabbit was placed on their side with the injected eye facing down for 3 hours to ensure attachment of the cells. On day 7, rabbits were anesthesized with an intramuscular injection of ketamine (40 mg/kg) and xylazine (4 mg/kg) and then euthanized with of Euthasol solution (1 mL/1.8 lbs.) containing (390 mg/mL Sodium Pentobarbitol, 50 mg/mL Phenytoin Sodium) through an ear vein injection. Eyes were immediately enucleated and intravitreal injected with 100 μL 2% paraformaldehyde (PBS++).
The whole eye was then immersed in 2% paraformaldehyde (PBS++) and fixed at 4° C. for 24 hours. After 24 hours the eye was rinsed in PBS and the cornea was excised and rinsed 3×'s for 5 minutes. The cornea was then incubated CE facing down on 1 mL of PBS++ containing 2 drops of NucBlue (Life Technologies) and 2:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) for 2 hours at room temperature. Corneas were then rinsed 3×'s for 5 minutes in PBS followed by 2 hour incubation on 1 mL PBS++ with 2:100 dilution of AlexaFluor 555 goat anti-mouse secondary antibody for 2 hours and stored in PBS before imaging on the Zeiss LSM700 confocal.
In an aspect, the tubule endothelial segments are generated by culturing endothelial cells on micropatterned fibronectin rectangles on PIPAAm, a thermosensitive polymer. Following dissolution of the PIPAAm, tubule segments are released, which are able to be incorporated into engineered cardiac tissues to create a primitive vascular network. Fibronectin rectangles are stamped onto PIPAAm. Endothelial cells are seeded on three different stamp patterns form a confluent monolayer on the rectangles at different seeding densities. Endothelial tubules are released from the PIPAAm surface using thermal control.
The tubule endothelial segments can be configured for treatments of cardiovascular disease (CVD). CVD is a significant global concern and the leading cause of mortality worldwide. CVD includes myocardial infarction, heart failure, and congenital heart defects, and can lead to the inability of the heart to supply blood to tissues throughout the body. Recent advances in tissue engineering have produced three-dimensional cardiac tissues from fibroblasts in combination with embryonic or induced pluripotential stem cell-derived cardiomyocytes. These engineered cardiac tissues could potentially be used to “patch” damaged areas of the heart. However, the heart is an extremely complex organ with multiple cellular types present, in addition to both fibroblasts and cardiomyocytes. The cell patches described here are configured to provide a supply of vascular tissues.
As shown in
Spin-Coating PDMS and PIPAAm onto Glass Coverslips
Glass coverslips were sonicated in a 50% v/v solution of ethanol to remove any particulates. The coverslips were then dried using nitrogen spray gun and placed in a large Petri dish. The PDMS used to spin-coat these coverslips was prepared by using a 1:10 ratio of Sylgard 184 to curing agent. This solution was then mixed until homogenous and put into a vacuum chamber to remove bubbles for 45 minutes. The coverslips were then added to the spin coater with a 200 μL droplet of PDMS placed in the center of the coverslip. The coverslips were spin-coated for 2 minutes at 2000 rpm. PDMS coated coverslips were cured in 65 degrees Celsius for 4 hours. Once complete, the coverslips were stored until further use.
Culturing HUVECs
HUVECs were taken from the liquid nitrogen room, allowed to thaw in the water bath, combined with 10 mL growth media, spun down to a pellet, and then aspirated and combined with more growth media to reach a concentration of 106 cells/mL. The cells were then allocated at a concentration of 5,000 cells/cm2 into 175 cm2 flasks. The media was replaced every other day until cells reached confluency approximately 80% confluency (at which point they were used for experiments).
Stamp onto Coated Coverslips Using Fibronectin
Fibronectin was prepared by initially creating a 50 μg/mL solution of unlabeled Fibronectin. This was done by combining 3.8 mL of deionized water with 0.2 mL of 1 mg/mL unlabeled fibronectin. From there, a 10% labeled Fibronectin solution was created through combining 900 μL of the 50 μg/mL solution of unlabeled Fibronectin, 92.5 μL of deionized water, and 7.5 μL of 667 μg/mL labeled 633-Fibronectin.
The PDMS stamps were added to a beaker filled with a solution of 50% ethanol solution and 50% distilled/deionized water. This beaker was placed in the sonicator for at least 30 minutes. The stamps were then picked up individually using sterile tweezers and dried using the nitrogen gun. The stamps were then placed in a sterile Petri dish to await Fibronectin coating. 300 μL of the Fibronectin solution was then pipetted onto each PDMS stamp with the stamp facing upwards, as shown in step 115. Using the pipet, fibronectin was spread to all four corners of the PDMS stamp and allowed to coat the stamps for at least one hour.
Nearing the end of this hour, the PDMS coated coverslips were placed in the UVO cleaner for 15 minutes with the lid placed below the dish inside the cleaner. Immediately after the 15 minutes, the Petri dish was placed within the biohood to maintain sterility. Using tweezers, each of the coverslips were placed into their own well of a 6-well culture plate.
The PDMS stamps were washed by quickly swirling them individually in a dish of deionized water and then washed once more in another dish of deionized water. The stamps were then dried using the nitrogen gun and then using a second pair of forceps to ensure good contact, the stamps were flipped stamp facing down onto the PDMS-coated coverslips, as shown in step 125. The stamps were gently tapped to ensure good contact with the coverslips. The stamps were then gently lifted off the coverslips without twisting to avoid disrupting the transferred pattern. The coverslips were then submerged in 1×PBS for storing purposes.
The protocol for stamping PIPAAm coated coverslips only differs in certain steps from the above protocol. This includes leaving the lid on the Petri dish during the UVO treatment and not using PBS for storage. Generally, stamps are left on the coverslips for at least 45 minutes and up to 24 hours.
Seeding onto PDMS Stamped coverslips As shown in step 135, the stamped coverslips' PBS was aspirated and then seeded with the HUVEC concentrations of 150,000 cells/cm2, 300,000 cells/cm2, and 450,000 cells/cm2.
Seeding onto PIPAAm Stamped coverslips In an aspect, at step 135, a heat-block was sprayed down with 70% ethanol and placed in the biohood. The left side was set to 52 degrees Celsius and the right side to 45 degrees Celsius. Stamped PIPAAm coverslips were secured to the bottom of their own Petri dishes using vacuum grease. The Petri dishes were then placed on the left side of the heat-block. Each coverslip needs 2 mL of fluid. The intended experimental cell concentrations were added in 15 mL tubes and placed in the right side of the heat-block for approximately five minutes or until a temperature between 38 to 40 degrees Celsius is accomplished. Coverslips achieved a temperature between 38 to 40 degrees Celsius. 2 mL of the cell solution was then added to each coverslip and placed immediately in the incubator to prevent any decreases in temperature that would cause the PIPAAm to prematurely dissolve.
Thermally Releasing the Substrates to Form the Endothelial Tubules
As step 145, the PIPAAm was thermally released through the process of adding room temperature media to the dishes while under the microscope. The cells were fixed through aspirating the media, washing with 1×PBS with calcium and magnesium, aspirating once more, ensuring that no cells are being aspirated, and then adding 4% Formaldehyde in the hood and waiting 30 minutes, at which point, the solution is aspirated carefully and the cells are submerged in 1×PBS and then stored.
The coverslips were the primary ZO-1 antibody (5:200 ratio) and then washed in 1×PBS three times with 30-minute increments in between. The coverslips were then stained with DAPI (1:100 ratio), 488 Phalloidin (3:200 ratio), and secondary goat anti-mouse 555 (5:200 ratio). The ZO-1 antibody stains for tight junctions between cells. These tight junctions are an indicator of high cell confluency which is vital in the formation of endothelial tubule segments.
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As previously mentioned, tight junctions are important junctional components present between endothelial cells within the tissue microvasculature. To determine whether tight junctions are present in HUVECs seeded on the micropatterned rectangles, an antibody is used that is targeted against ZO-1. This antibody is a marker of tight junctions. In samples with lowest seeding density, very low levels of ZO-1 was observed, as shown in image 500a of
In an aspect, based on the images previously described, an example optimal cell concentration is about 300,000 cells/cm2. Generally, 450,000 cells/cm2 was too high in all three micro-patterned dimensions. The 200×20 μm micro-patterned rectangles have the most confluent cells compared to the 200×10 μm size, which tended to have cells spread out beyond the micro-patterned rectangles.
The CE is the single layer of cells that lines the posterior surface of the cornea and is responsible for maintaining proper corneal thickness and clarity by pumping excess fluid out of the stroma into the anterior chamber. The cells of the CE are arrested in the GI phase of the cell cycle and therefore cannot replicate to repair damage due to disease, injury, or normal aging. As a result, when cells die, the remaining cells become larger to maintain the integrity and pump function of the monolayer, decreasing the cell density. Once the cell density drops below ˜500 cells/millimeter (mm)2, the CE can no longer properly function, causing excess fluid to build up in the corneal stroma and resulting in corneal blindness. The structure (cell mono layer), function (barrier/fluid pumping), and location (adjacent to the anterior chamber) of the CE make it an ideal target for cell injection therapy compared to more complex tissues. Still, animal models and clinical studies have shown that injection in to the anterior chamber for CE repair suffers from the lack of cell retention, viability and integration into the CE, requiring the injection of large numbers of cells and small molecules such as the ROCK inhibitor Y-27632, in order to achieve desirable results. However, even the best results are still limited in their regenerative capabilities, which is because cell-cell junctions and cytoskeletal structure, for CE cells in particular, have been shown to be necessary for cell signaling, function and maintenance of a mature, non-proliferative monolayer state through contact inhibition.
This embodiment describes shrink-wrapping (e.g., encapsulation) of micron-scale monolayers of CE cells (CECs) in the nanometer thick ECM scaffolds according to the encapsulation method described in relation to
This description describes experimental results in which shrink-wrapped μMonolayers were injected into the anterior chamber of rabbit eyes in vivo to demonstrate in vivo integration into a CE to increase cell density. Experimentation has shown that shrink-wrapping CEC μMonolayers in ECM scaffolds maintains cell cytoskeletal structure, tight junctions and viability post-injection.
To engineer monolayers of CECs with micron scale dimensions (μMonolayers), modifications were made for shrink-wrapped single cells (termed SHELLs) in nanometer thick ECM scaffolds. Here, the dimensions of the squares that are patterned on poly(N-isopropylacrylamide) (PIPAAm) via surface-initiated assembly techniques are increased in size, to ˜200 μm×200 μm. The increased size of the scaffolds allows for more cells to adhere to each scaffold. The culture time of the cells on the squares to 24 hours is increased to approximately 24 hours. Because PIPAAm is thermoresponsive, samples and cells were heated to 40° C. during the seeding process to ensure the PIPAAm did not dissolve.
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Images 900b show that the release and shrink-wrapping of the CECs occurs quickly, in <100 seconds once the sample cools to room temperature. Upon dissolution of the PIPAAm and thermal release, the μMonolayers remained intact and were successfully shrink-wrapped within the ECM squares. Once released, the shrink-wrapped μMonolayers were collected, centrifuged, injected through a 280 needle and allowed to settle for 30 min before fixing and staining to investigate the structure of the BCECs within the μMonolayers. As seen in images 900c, shrink-wrapped BCECs exhibited continuous ZO-1 at the borders and a cortical F-actin structure indicating that the cells maintained their tight-junctions and cytoskeletal structure throughout the release process. Confocal microscopy images show that after injection, the CECs maintain both their cytoskeletal structure (F-actin) and tight junctions (ZO-1).
Image 900d includes a 3D projection of a shrink-wrapped CEC monolayer 30 minutes after injection illustrating how it begins to relax and return to its original shape. Image 900d shows that post-release, the shrink-wrapped μMonolayers began to relax and return to their as engineered square like structure approximately 30 minutes after injection. Viability of the cells within the μMonolayers after injection was examined using a live/dead cytotoxicity assay and compared to enzymatically-released single cells. Image 900e shows representative live/dead images of control single CECs and shrink-wrapped CECs show that both types of cells are viable with very few dead cells present. Confocal microscopy images indicated that the only dead cells in the shrink-w rapped μMonolayer samples were individual cells that were not integrated into μMonolayers.
Live/dead data (n=3) was statistically compared using a Student's t-test and no significant difference in viability between single cells and shrink-wrapped cells injected through a 28G needle was observed with both types exhibiting over 90% viability. Image 900f shows that quantification determined that single CECs had greater than 93% viability and shrink-wrapped cells (including the cells not integrated within the μMonolayers) had greater than 97% viability.
Shrink-wrapped μMonolayers display different growth characteristics compared to single CECs. To assess the potential use of shrink-wrapped μMonolayers in cell injection therapy, it was determined whether they would attach to and proliferate on a denuded stroma, which is primarily collagen I. Both single CECs and shrink-w rapped μMonolayers were injected onto compressed collagen I gels that served as a denuded stromal mimic. Samples were fixed and stained at 6 and 24 hours post-inject ion to observe the cell structure and outgrowth.
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Images 1000b show a comparison between single CECs (top image) and wrapped monolayers (bottom image). The 3D views of the cells at 6 hours post seeding, show the differences between the single CECs and shrink-wrapped CECs. Examining the samples in 3D confirmed that single CECs were very rounded and had very little interactions between cells, whereas the shrink-wrapped uMonolayers had inverted with the ECM scaffolds now present within the center of the cells which were directly attached to the stromal mimic (1000b, bottom). After 24 hours, the single CECs covered most of the stromal mimic and had a more defined cytoskeletal structure compared to the CECs at 6 hours with many F-actin stress fibers across the cell bodies. Images 1000c show that, at 24 hours, single CECs have begun to spread and cover almost the entire scaffold. At 24 hours, the CECs have already grown out of the ECM scaffolds and formed an almost complete monolayer. In images 1000d, the nucleus is dark (shaded blue), ZO-1 is light (shaded red), COL4 is shaded magenta, and F-actin is shaded green. The scale bars are 50 μm except in the orthogonal views, in which the scale bars are 20 μm. The single CECs exhibited very little, discontinuous ZO-1 at the cell borders. In contrast, the shrink-wrapped uMonolayers had continuous ZO-1 at all cell borders and cortical F-actin cytoskeleton, which closely resembled the structure of in vivo CECs, as shown in images 1000d. The ECM scaffolds were still visible after 24 hours as indicated by the arrows in image 1000d (right image).
Shrink-wrapped uMonolayers integrate into CE monolayers and increase density in vivo. Many patients need corneal transplants due to failure caused by low cell density and not disease and therefore could benefit from an injection of cells to boost cell density without a complete replacement. Therefore, the integration of shrink-wrapped CECs into existing CE monolayers is performed. Low density CE monolayers were formed by seeding late passage BCECs on the collagen I gel stromal mimics used in the previous experiments. Shrink-wrapped uMonolayers or the equivalent number of single CECs were then injected through a 300 needle on to confluent CE monolayers. The CECs used for both the single cell and uMonolayers injections were labeled with CellTracker green to track the cells post-injection and CE monolayers that were not injected with any cells served as controls. Samples were fixed, stained and analyzed at 3, 7 and 14 days post injection. Images of each of these data points are shown in
Confocal images of shrink-wrapped μMonolayers integrating at each time point were quantified using a heat map representation. The average pixel density of the CellTracker signal for each integrating μMonolayer was calculated and results are displayed in the heat maps shown in images 1100b. These heat maps confirm the observation that the shrink-wrapped CEC μMonolayers initially integrate in a densely packed cluster that spreads out over time. The heat maps of the area occupied by Cell Tracker labeled shrink-wrapped cells show that over the course of 14 days indicate that the cells initially integrate in a tight cluster and then the density starts to equilibrate as the cells spread out slightly. For example, for day 3 the number is 33, for day 7, the number is 37, and for day 14, the number is 40.
To quantify if shrink-wrapped μMonolayers increased the cell density of the CE monolayers, confocal images were obtained and the cell density was determined by counting the nuclei per image and dividing by the image area for each sample type at each time point. Ten images per sample were counted to obtain an average per sample and the average per sample type was calculated from either 3 or 4 individual samples and results are shown in graph 1100c. At day 3, the CE monolayers with integrated shrink-wrapped μMonolayers (1731±267 cells/mm 2 had a significantly higher cell density compared to controls (1016±75 cells/mm 2) but there was no significant difference compared to CE monolayers with integrated single CECs (1253±31 cells/mm 2). At day 7, the CE monolayers shrink-wrapped μMonolayers integrated (1631±58 cells/mm 2) had a significantly higher density compared to both controls (989±11 cells/mm 2 and monolayers with single CECs integrated (1220±56 cells/mm 2). Similarly, at day 14, the CE monolayers with shrink-wrapped μMonolayers integrated (1545±95 cells/mm2 had a significantly higher density compared to both controls (994±104 cells/mm 2) and monolayers with single CECs integrated (1224±66 cells/mm). The large standard deviation in cell density at day 3 observed in the shrink-wrapped samples decreased at day 7 and 14 further confirmed the observations made qualitatively from the confocal images and heat maps. This continued integration and equilibration also explains the perceived decrease in cell density from day 3 to 14, where the cell density overall isn't necessarily decreasing, it is just becoming more homogenous across the CE monolayer.
Although these long-term results are useful, it was also important to understand the short-term integration of the shrink-wrapped CECs to determine if method used clinically for single cell injections, keeping the patient face down for 3 hours, would be sufficient for injection of shrink-wrapped μMonolayers. Image 1100d of
As a next step to confirm these results before moving to in vivo studies, shrink-wrapped rabbit μMonolayers were injected into the anterior chamber of ex vivo rabbit eyes and incubated the eyes cornea down for 3 hours before fixation of the whole globe. Post fixation, the cornea was excised and rinsed vigorously 3 times to remove any non-adhered cells and stained for ZO-1, F-actin and the nucleus. Confocal imaging of the corneas showed numerous shrink-wrapped μMono layers attached over the entirety of the corneas. The shrink-wrapped μMonolayers were still rounded and clustered with the ECM scaffold in the center, as shown in image 1200 of
A number of exemplary embodiments have been described. Nevertheless, it will be understood by one of ordinary skill in the art that various modifications may be made without departing from the spirit and scope of the techniques described herein.
This application claims priority under 35 U.S.C. § 119(e) to U.S. Patent Application Ser. No. 62/973,695, filed on Oct. 18, 2019, the entire contents of which are hereby incorporated by reference.
This invention was made with government support under Grant No. 1R01EY024642-01A1, awarded by the National Institutes of Health. The government has certain rights in the invention.
Filing Document | Filing Date | Country | Kind |
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PCT/US2020/056327 | 10/19/2020 | WO |