Claims
- 1. An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule stimulated ATPase activity; and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 2. An isolated nucleic acid sequence of claim 1, wherein the protein specifically binds to polyclonal antibodies to a protein comprising SEQ ID NO:2 or SEQ ID NO:4.
- 3. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid encodes SEQ ID NO:2 or SEQ ID NO:4.
- 4. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid has a nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:3.
- 5. An isolated nucleic acid sequence of claim 1, wherein the nucleic acid selectively hybridizes under stringent hybridization conditions to SEQ ID NO:1 or SEQ ID NQ:3.
- 6. An expression vector comprising a nucleic acid encoding a microtubule motor protein, wherein the motor protein has the following properties: (i) the protein's activity includes microtubule stimulated ATPase activity, and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 7. A host cell transfected with the vector of claim 6.
- 8. An isolated microtubule motor protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 9. An isolated protein of claim 8, wherein the protein specifically binds to polyclonal antibodies to HsKip3d.
- 10. An isolated protein of claim 8, wherein the protein is HsKip3d.
- 11. An isolated protein of claim 8, wherein the protein has an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.
- 12. An isolated protein of claim 8, wherein the protein specifically binds to polyclonal antibodies generated against a motor domain of HsKip3d.
- 13. An isolated protein of claim 8, wherein the protein comprises an amino acid sequence of a HsKip3d motor domain.
- 14. A method for screening for modulators of HsKip3d, the method comprising the steps of:
(i) providing biologically active HsKip3d, wherein the HsKip3d has the following properties: (i) activity including microtubule stimulated ATPase activity; and (ii) sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm; (ii) contacting biologically active HsKip3d with a candidate agent in a test and control concentration; and (iii) assaying for the level of HsKip3d activity, wherein the HsKip3d activity is selected from the group consisting of binding activity or ATPase activity, and wherein a change in activity between the test and control concentration indicates a modulator.
- 15. A method of claim 14, wherein the screening occurs in a multi-well plate as part of a high-throughput screen.
- 16. A method of claim 14, wherein the biologically active HsKip3d comprises an amino acid sequence of a HsKip3d motor domain.
- 17. A compound that modulates HsKip3d, wherein said compound is identified using the method of claim 14.
- 18. An isolated nucleic acid comprising a sequence which has greater than 60% sequence identity with nucleotide SEQ ID NO:1 or SEQ ID NO:3.
Priority Claims (1)
Number |
Date |
Country |
Kind |
09/632344 |
Aug 2000 |
US |
|
CROSS-REFERENCE TO RELATED APPLICATION
[0001] The present application is a continuation-in-part of U.S. patent application Ser. No. 09/632,344 filed Aug. 3, 2001 which is incorporated by reference in its entirety for all purposes.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US01/24285 |
8/3/2001 |
WO |
|