Claims
- 1. A kit for screening for modulators of a motor protein, comprisinga protein, wherein said protein comprises an amino acid sequence having greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm, wherein said protein has microtubule stimulated ATPase activity; and instructions for testing for activity of said protein.
- 2. The kit of claim 1, wherein the protein has greater than 95% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 3. The kit of claim 1, wherein the protein has greater than 98% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.
- 4. The kit of claim 1, wherein the protein comprises an amino acid sequence of SEQ ID NO:2.
- 5. The kit of claim 1, further comprising reaction tubes.
- 6. The kit of claim 1, further comprising a stationary multiwell plate.
- 7. The kit of claim 6, wherein the stationary multiwell plate is a 384-well microtiter plate.
- 8. The kit of claim 1, further comprising an enzyme system for monitoring ADP or phosphate level.
- 9. The kit of claim 8, wherein the enzyme system comprises pyruvate kinase and lactate dehydrogenase.
- 10. The kit of claim 8, wherein the enzyme system comprises luciferin-luciferase system.
Parent Case Info
This application is a continuation of Ser. No. 09/632,155, filed Aug. 03, 2000, now U.S. Pat. No. 6,429,005.
Non-Patent Literature Citations (3)
Entry |
Nakagawa et al. [PNAS USA, 94(18) : 9654-59, 1997].* |
Nakagawa et al. “Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome”, 1997 Proc. Natl. Acad. Sci. U.S.A. 94 (18) 9654-9659. |
GenBank, Direction Submission, Accession No. AB00148, Nus Musculus MRNA for Motor domain of Kif12. |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/632155 |
Aug 2000 |
US |
Child |
10/170110 |
|
US |