Claims
- 1. A kit for screening for a modulator of a motor protein, comprising(a) the motor protein, wherein the motor protein (i) comprises an amino acid sequence having greater than 90% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, and (ii) has microtubule stimulated ATPase activity; and (b) instructions for testing for ATPase activity of the motor protein.
- 2. The kit of claim 1, wherein the motor protein has greater than 95% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 3. The kit of claim 1, wherein the motor protein has greater than 98% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 4. The kit of claim 1, wherein the motor protein comprises the amino acid sequence of SEQ ID NO:2.
- 5. The kit of claim 1, wherein the motor protein comprises the amino acid sequence of SEQ ID NO:4.
- 6. The kit of claim 1, further comprising reaction tubes.
- 7. The kit of claim 1, further comprising a stationary multiwell plate.
- 8. The kit of claim 7 wherein the stationary multiwell plate is a 384-well microtiter plate.
- 9. The kit of claim 1, further comprising an enzyme system for monitoring ADP or phosphate level.
- 10. The kit of claim 9, wherein the enzyme system comprises pyruvate kinase and lactate dehydrogenase.
- 11. The kit of claim 9, wherein the enzyme system comprises a luciferin-luciferase system.
CROSS-REFERENCES TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 09/721,251, filed Nov. 22, 2000 now U.S. Pat. No. 6,610,525, the disclosure of which is incorporated herein by reference.
US Referenced Citations (5)
Number |
Name |
Date |
Kind |
6207403 |
Goldstein et al. |
Mar 2001 |
B1 |
6284480 |
Nislow et al. |
Sep 2001 |
B1 |
6335189 |
Beraud et al. |
Jan 2002 |
B1 |
6395540 |
Beraud et al. |
May 2002 |
B1 |
6410254 |
Finer et al. |
Jun 2002 |
B1 |
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO9911814 |
Mar 1999 |
WO |
Non-Patent Literature Citations (2)
Entry |
US patent application No. 09/314,464, Finer et al., Filed May 18, 1999, Title: Compositions And Assays Utilizing ADP Or Phosphate For Detecting Protein Modulators. |
Hanlon et al., “Characterization of KIFC2, a Neuronal Kinesin Superfamily Member in Mouse”, Neuron, vol. 18, pp 439-451, Mar. 1997. |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/721251 |
Nov 2000 |
US |
Child |
10/114764 |
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US |