Claims
- 1. An isolated mammalian T-box transcription factor polypeptide encoded by a nucleic acid molecule which hybridizes to a complement of a nucleic acid molecule comprising SEQ ID NO:1 at 6.times. SSC at 45.degree. C., followed by one or more washes in 0.2.times. SSC, 0.1% SDS at 50-65.degree. C.
- 2. An isolated mammalian T-box transcription factor polypeptide comprising an amino acid sequence which is at least 90% identical to the amino acid sequence of SEQ ID NO:2, wherein said percent identity is calculated using the ALIGN program for comparing amino acid sequences, a PAM 120 weight residue table, a gap length penalty of 12, and a gap penalty of 4.
- 3. An isolated mammalian T-box transcription factor polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence having 1-5% variance as compared to the nucleotide sequence of SEQ ID NO:1, wherein said 1-5% variance results in an amino acid substitution at a non-essential amino acid residue of the polypeptide.
- 4. An isolated mammalian T-box transcription factor polypeptide comprising the amino acid sequence of SEQ ID NO:2 within which a conservative amino acid substitution has been made.
- 5. An isolated mammalian T-box transcription factor which is encoded by the nucleotide sequence contained in the plasmid deposited with ATCC.RTM. as Accession Number 209973 or a complement thereof.
- 6. An isolated mammalian T-box transcription factor polypeptide encoded by a nucleic acid molecule which hybridizes to a complement of a nucleic acid molecule consisting of SEQ ID NO:1 at 6.times. SSC at 45.degree. C., followed by one or more washes in 0.2.times. SSC, 0.1% SDS at 50-65.degree. C.
- 7. An isolated mammalian T-box transcription factor polypeptide consisting of an amino acid sequence which is at least 90% identical to the amino acid sequence of SEQ ID NO:2, wherein said percent identity is calculated using the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4.
- 8. An isolated mammalian T-box transcription factor polypeptide which is encoded by a nucleic acid molecule consisting of a nucleotide sequence having 1-5% variance as compared to the nucleotide sequence of SEQ ID NO:1, wherein said 1-5% variance results in an amino acid substitution at a non-essential amino acid residue of the polypeptide.
- 9. An isolated mammalian T-box transcription factor polypeptide consisting of the amino acid sequence of SEQ ID NO:2 within which a conservative amino acid substitution has been made.
- 10. An isolated polypeptide comprising at least 15 consecutive amino acid residues of the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:6.
- 11. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.
- 12. An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO:2.
- 13. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:6.
- 14. An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO:6.
- 15. An isolated polypeptide comprising at least 50 consecutive amino acid residues of the amino acid sequence of SEQ ID NO:2.
- 16. An isolated polypeptide comprising at least 50 consecutive amino acid residues of the amino acid sequence of SEQ ID NO:6.
- 17. An isolated fusion polypeptide comprising the polypeptide of any one of claims 1-5, 11, 13, 15, or 16, operatively linked to a heterologous polypeptide.
RELATED APPLICATIONS
This application is a continuation-in-part of U.S. patent application Ser. No. 09/163,116, filed Sep. 29, 1998, which claims priority to U.S. Provisional patent application Ser. No. 60/089,467, filed Jun. 16, 1998, and of U.S. patent application Ser. No. 09/188,811 (MNI-046CP), filed Nov. 9, 1998, the contents of which are incorporated herein by reference.
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
163116 |
Sep 1998 |
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