MULLERIAN INHIBITING SUBSTANCE

Information

  • Research Project
  • 3491421
  • ApplicationId
    3491421
  • Core Project Number
    R43CA042340
  • Full Project Number
    1R43CA042340-01
  • Serial Number
    42340
  • FOA Number
  • Sub Project Id
  • Project Start Date
    2/28/1986 - 38 years ago
  • Project End Date
    8/14/1986 - 38 years ago
  • Program Officer Name
  • Budget Start Date
    2/28/1986 - 38 years ago
  • Budget End Date
    8/14/1986 - 38 years ago
  • Fiscal Year
    1986
  • Support Year
    1
  • Suffix
  • Award Notice Date
    -
Organizations

MULLERIAN INHIBITING SUBSTANCE

Mullerian Inhibiting Substance, a product of the fetal testis, is a large molecular weight glycoprotein which initiates regression of the Mullerian duct, the anlagan of the uterus, fallopian tube, and upper vagina. Donahoe and co-workers have shown that this fetal regressor can also inhibit the growth of some tumors derived from the cell types which respond to the fetal regressor in embryonic life. Biologically active MIS has been purified 30,000 fold from bovine testis. However, the amount of bovine MIS that can be isolated by conventional means is too low to permit broad oncological studies, and the human protein is not available. We propose to use recombinant DNA technology to isolate the genes for bovine and human MIS and to express those genes in E. coli or animal cells. We will then develop a process for purifying large amounts of human MIS in order to test the efficacy of MIS as an anti-cancer drug in a clinical setting. Specifically in Phase I, we propose to sequence peptides of bovine MIS and generate oligonucleotide probes. The probes will be used to isolate the bovine clone from a cDNA library, which will then be used to isolate the human gene.

IC Name
NATIONAL CANCER INSTITUTE
  • Activity
    R43
  • Administering IC
    CA
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    395
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    BIOGEN, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    CAMBRIDGE
  • Organization State
    MA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    02142
  • Organization District
    UNITED STATES