The present disclosure relates to a contrast agent having a plurality of modalities such that it can be used for a plurality of imaging methods.
This section provides background information related to the present disclosure which is not necessarily prior art.
A contrast agent is a compound used to increase the contrast of naturally occurring or administered cells, structures, or fluids within a body during an imaging procedure. Contrast agents may have various properties depending on the application, location, or imaging technique.
Magnetic resonance imaging (MRI) is a medical imaging technique used to form anatomical images of a subject and/or images of physiological processes in the subject. In MRI, a strong magnetic field is created around an area to be imaged within an MRI scanner. Targeting a selected region, the magnetic field is applied to the subject at a resonant frequency. Atoms of the targeted area are excited and emit a radio frequency signal that is measured by a receiving coil. The signal received by the coil is processed to determine information regarding the scanned area. Contrast agents that interact with the MRI scanner may be given to the subject to make the image clearer.
Magnetic particle imaging (MPI) is an imaging technique that measures a three-dimensional location and concentration of nanoparticle contrast agents to form images. MPI systems use changing magnetic fields to generate a signal from superparamagnetic iron oxide nanoparticles. The changing magnetic fields are used to produce a single magnetic field free region that is moved across a sample. As superparamagnetic iron oxide is not naturally present within a body, a signal is only generated from the administered agent, thus creating an image without background.
Computed tomography (CT), also referred to as computerized axial tomography (CAT), is an imaging technique that uses computer-processed combinations of many X-ray measurements taken from different angles to produce cross-sectional images of specific areas of a scanned object. A contrast agent dye can aid in the visualization of certain structures of interest. CT scans produce data that can be manipulated to demonstrate various bodily structures based on their ability to absorb the X-ray beam.
Positron emission tomography (PET) is a technique that uses radioactive substances to image and measure metabolic processes in the body. PET images are used to detect or measure changes in physiological activities, such as metabolism, blood flow, regional chemical composition, and absorption. The contrast agent used in PET includes radioactive materials that are trapped within tissues of interest. The unstable nucleus of radioligands emits positrons, which combine with neighboring electrons to generate gamma rays. The gamma rays are detected and recorded. Computer reconstruction of the recording produces a three-dimensional image.
Single-photon emission computed tomography (SPECT) is another nuclear medicine tomographic imaging technique that utilizes gamma rays. In SPECT, a radioisotope is attached to a ligand to create a radioligand contrast agent that targets and binds to specific tissues. SPECT is similar to PET in its use of a radioactive material and detection of gamma rays. However, unlike PET, which measures gamma rays produced when positrons contact and combine with electrons, in SPECT, the radioactive material emits gamma radiation that is measured directly.
Fluorescence (FL) imaging is a technique that photographs fluorescent dies and fluorescent proteins, the contrast agents, for visualizing molecular mechanisms and structures. When a certain molecule absorbs light, the energy of the molecule is briefly raised to a higher excited state. The subsequent return to ground state results in an emission of fluorescent light that can be detected and measured.
Photoacoustic (PA) imaging is a technique based on the photoacoustic effect. In PA imaging, after administering a contrast agent, such as a dye, nanostructures comprising gold or carbon, and liposome encapsulations, non-ionizing laser pulses are delivered into tissue where some of the delivered energy is absorbed by the tissue and the contrast agent and converted into heat. The heat leads to transient thermoelastic expansion and wideband ultrasonic emission. Generated ultrasonic waves are detected by ultrasonic transducers, and images are generated from the ultrasonic waves.
Because imaging techniques have differing modes of operation, different contrast agents with specific properties often need to be used to perform different scans in a single subject. Accordingly, a multi-modal contrast agent that can be detected through a plurality of imaging techniques is desired.
This section provides a general summary of the disclosure, and is not a comprehensive disclosure of its full scope or all of its features.
The present disclosure relates to a multi-modal contrast agent for medical imaging.
In various aspects, the current technology provides a nanoparticle including a magnetic core having a magnetic nanocrystal; a fluorophore coupled to the magnetic core; at least one chelating compound coupled to the magnetic core, the at least one chelating compound being a compound that chelates copper-64 (64Cu), a compound that chelates technetium-99m (99mTc), or a combination thereof; and an iodine chelator coupled to the magnetic core.
In one aspect, the magnetic nanocrystal includes iron oxide and the magnetic core has a diameter of greater than or equal to about 1 nm to less than or equal to about 50 nm.
In one aspect, the nanoparticle includes a plurality of magnetic cores, wherein each magnetic core of the plurality includes a plurality of magnetic nanocrystals.
In one aspect, the magnetic core is at least partially coated with dextran.
In one aspect, the fluorophore, the at least one chelating compound, and the iodine chelator are indirectly coupled to the magnetic core by way of the dextran.
In one aspect, the fluorophore is selected from the group consisting of a cyanine dye, a coumarin, a rhodamine, a xanthene, a quantum dot, derivatives thereof, and combinations thereof.
In one aspect, the nanoparticle includes the compound that chelates 64Cu, the compound that chelates 64Cu being S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester), 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA), or combinations thereof.
In one aspect, the nanoparticle includes the compound that chelates 99mTc, the compound that chelates 99mTc being [(R)-2-amino-3-(4-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-pentaacetic acid (SCN-Bn-CHx-DTPA).
In one aspect, the iodine chelator is thyroxine (T4), triiodothyronine (T3), or a combination thereof.
In one aspect, the fluorophore, the compound that chelates 64Cu, the compound that chelates 99mTc, or the combination thereof and the iodine chelator are coupled to the magnetic core by a linker, the linker being aminated epichlorohydrin.
In one aspect, the iodine chelator is further coupled to the core by succinimidyl 6-((beta-maleimidopropionamido)hexanoate) (SMPH) and succinimidyl 3-(2-pyridyldithio)propionate (SPDP).
The current technology also provides a nanoparticle including a single magnetic core having a diameter of greater than or equal to about 20 nm to less than or equal to about 30 nm, the single magnetic core including at least one iron oxide nanocrystal; a coating at least partially covering the single magnetic core, the coating including dextran cross-linked with aminated epichlorohydrin; and an iodine chelator coupled to the aminated epichlorohydrin, the iodine chelator including T4, T3, or a combination thereof, wherein the nanoparticle is configured to provide contrast for MRI, MPI, CT, and X-ray imaging.
In one aspect, the nanoparticle further includes a fluorophore coupled to the single magnetic core, wherein the nanoparticle is further configured to provide contrast for at least one of FL imaging, PA imaging, and ultrasound (US) imaging.
In one aspect, the nanoparticle further includes a compound that chelates 64Cu coupled to the single magnetic core, the compound that chelates 64Cu being selected from the group consisting of S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester), 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA), and combinations thereof, wherein the nanoparticle is further configured to provide contrast for PET.
In one aspect, the nanoparticle further includes a compound that chelates 99mTc coupled to the single magnetic core, the compound that chelates 99mTc being [(R)-2-amino-3-(4-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-pentaacetic acid (SCN-Bn-CHx-DTPA), wherein the nanoparticle is further configured to provide contrast for SPECT.
In one aspect, the nanoparticle further includes a fluorophore coupled to the single magnetic core; a compound that chelates 64Cu coupled to the single magnetic core, the compound that chelates 64Cu being selected from the group consisting of S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester), 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA), and combinations thereof; and a compound that chelates 99mTc coupled to the single magnetic core, the compound that chelates 99mTc being [(R)-2-amino-3-(4-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-pentaacetic acid (SCN-Bn-CHx-DTPA), wherein the nanoparticle is further configured to provide contrast for PET, SPECT, and at least one of FL imaging, PA imaging, or US imaging.
The current technology yet further provides a method of making the nanoparticle, the method including reacting iron(II) chloride (FeCl2) with potassium hydroxide (KOH) in water under the flow of an inert gas to form a reaction mixture, adding hydrogen peroxide (H2O2) to the reaction mixture to form a plurality of magnetic cores in the reaction mixture, applying a magnetic field to the reaction mixture and isolating the plurality of magnetic cores, contacting the plurality of magnetic cores with dextran to form a dextran coating at least partially covering each magnetic core of the plurality, cross-linking the dextran with a plurality of epichlorohydrin molecules, aminating the plurality of epichlorohydrin molecules to form the coating including dextran cross-linked with aminated epichlorohydrin on each magnetic core of the plurality, and conjugating a first portion of the aminated epichlorohydrin molecules on at least a portion of the plurality of magnetic cores with the iodine chelator.
In one aspect, the method further includes conjugating a second portion of the aminated epichlorohydrin molecules with a fluorophore, conjugating a third portion of the aminated epichlorohydrin molecules with a compound that chelates 64Cu, and conjugating a fourth portion of the aminated epichlorohydrin molecules with a compound that chelates 99mTc.
In yet various other aspects, the current technology provides a method of imaging at least one of a tissue or a metabolic process in a subject, the method including administering to the subject a safe and effective amount of a composition including a contrast agent, wherein the contrast agent associates with the at least one of the tissue or the metabolic process, the contrast agent including a magnetic core including a magnetic nanocrystal, a fluorophore coupled to the magnetic core, at least one chelating compound coupled to the magnetic core, the at least one chelating compound being a compound that chelates 64Cu, a compound that chelates 99mTc, or a combination thereof, and an iodine chelator coupled to magnetic core; performing at least one of MRI, MPI, FL imaging, PA imaging, US imaging, PET, SPECT, X-ray imaging, or CT; and generating an image of the tissue or the metabolic process with contrast provided by the contrast agent.
In one aspect, the administering is performed intravenously, intraductally, intrathecally, intramuscularly, intratumorally, or orally.
In one aspect, the composition further includes a pharmaceutically acceptable carrier.
In one aspect, the method includes performing at least two of the MRI, the MPI, the FL imaging, the PA imaging, the US imaging, the PET, the SPECT, the X-ray imaging, or the CT and generating at least two images of the tissue or the metabolic process, each of the at least two images having contrast provided by the contrast agent.
Further areas of applicability will become apparent from the description provided herein. The description and specific examples in this summary are intended for purposes of illustration only and are not intended to limit the scope of the present disclosure.
The drawings described herein are for illustrative purposes only of selected embodiments and not all possible implementations, and are not intended to limit the scope of the present disclosure.
Corresponding reference numerals indicate corresponding parts throughout the several views of the drawings.
Example embodiments are provided so that this disclosure will be thorough, and will fully convey the scope to those who are skilled in the art. Numerous specific details are set forth such as examples of specific compositions, components, devices, and methods, to provide a thorough understanding of embodiments of the present disclosure. It will be apparent to those skilled in the art that specific details need not be employed, that example embodiments may be embodied in many different forms and that neither should be construed to limit the scope of the disclosure. In some example embodiments, well-known processes, well-known device structures, and well-known technologies are not described in detail.
The terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting. As used herein, the singular forms “a,” “an,” and “the” may be intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms “comprises,” “comprising,” “including,” and “having,” are inclusive and therefore specify the presence of stated features, elements, compositions, steps, integers, operations, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. Although the open-ended term “comprising,” is to be understood as a non-restrictive term used to describe and claim various embodiments set forth herein, in certain aspects, the term may alternatively be understood to instead be a more limiting and restrictive term, such as “consisting of” or “consisting essentially of.” Thus, for any given embodiment reciting compositions, materials, components, elements, features, integers, operations, and/or process steps, the present disclosure also specifically includes embodiments consisting of, or consisting essentially of, such recited compositions, materials, components, elements, features, integers, operations, and/or process steps. In the case of “consisting of,” the alternative embodiment excludes any additional compositions, materials, components, elements, features, integers, operations, and/or process steps, while in the case of “consisting essentially of,” any additional compositions, materials, components, elements, features, integers, operations, and/or process steps that materially affect the basic and novel characteristics are excluded from such an embodiment, but any compositions, materials, components, elements, features, integers, operations, and/or process steps that do not materially affect the basic and novel characteristics can be included in the embodiment.
Any method steps, processes, and operations described herein are not to be construed as necessarily requiring their performance in the particular order discussed or illustrated, unless specifically identified as an order of performance. It is also to be understood that additional or alternative steps may be employed, unless otherwise indicated.
When a component, element, or layer is referred to as being “on,” “connected to,” or “coupled to” another element, it may be directly on, connected or coupled to the other element, or intervening elements may be present. In contrast, when an element is referred to as being “directly on,” “directly connected to,” or “directly coupled to” another element or layer, there may be no intervening elements present. Other words used to describe the relationship between elements should be interpreted in a like fashion (e.g., “between” versus “directly between,” “adjacent” versus “directly adjacent,” etc.). As used herein, the term “and/or” includes any and all combinations of one or more of the associated listed items.
Although the terms first, second, third, etc. may be used herein to describe various steps, elements, components, regions, layers and/or sections, these steps, elements, components, regions, layers and/or sections should not be limited by these terms, unless otherwise indicated. These terms may be only used to distinguish one step, element, component, region, layer or section from another step, element, component, region, layer or section. Terms such as “first,” “second,” and other numerical terms when used herein do not imply a sequence or order unless clearly indicated by the context. Thus, a first step, element, component, region, layer or section discussed below could be termed a second step, element, component, region, layer or section without departing from the teachings of the example embodiments.
Throughout this disclosure, the numerical values represent approximate measures or limits to ranges to encompass minor deviations from the given values and embodiments having about the value mentioned as well as those having exactly the value mentioned. Other than in the working examples provided at the end of the detailed description, all numerical values of parameters (e.g., of quantities or conditions) in this specification, including the appended claims, are to be understood as being modified in all instances by the term “about” whether or not “about” actually appears before the numerical value. “About” indicates that the stated numerical value allows some slight imprecision (with some approach to exactness in the value; approximately or reasonably close to the value; nearly). If the imprecision provided by “about” is not otherwise understood in the art with this ordinary meaning, then “about” as used herein indicates at least variations that may arise from ordinary methods of measuring and using such parameters. For example, “about” may comprise a variation of less than or equal to 5%, optionally less than or equal to 4%, optionally less than or equal to 3%, optionally less than or equal to 2%, optionally less than or equal to 1%, optionally less than or equal to 0.5%, and in certain aspects, optionally less than or equal to 0.1%.
In addition, disclosure of ranges includes disclosure of all values and further divided ranges within the entire range, including endpoints and sub-ranges given for the ranges.
Example embodiments will now be described more fully with reference to the accompanying drawings.
Because imaging techniques have differing modes of operation, different contrast agents with specific properties often need to be used to perform different imaging methods in a single subject. Typically, when a subject is in need of imaging, a contrast agent is administered to the subject, wherein the contrast agent is configured to provide contrast for a specific imaging method. The specific imaging method is then performed. If the subject is in need of a second different type of imaging, a second contrast agent is administered to the subject, wherein the second contrast agent is configured to provide contrast for a second specific imaging method that is different form the first specific imaging method. The second imaging method is then performed. This pattern is repeated for each different type of imaging that is performed on the subject. When more than one type of imaging is desired, separate administering and imaging steps require long periods of time and may result in added stress and anxiety to the subject. Accordingly, the current technology provides a multi-modal contrast agent that can be detected through a plurality of imaging techniques after a single administration. The multi-modal contrast agent has a magnetic nanoparticle core and detectable agents coupled to the magnetic nanoparticle core.
With reference to
The nanoparticle 10 further comprises a coating 16 covering the at least one magnetic core 14. The coating 16 at least partially covers the at least one magnetic core 14 and may substantially cover the entirety of the at least one magnetic core 14. The term “substantially cover the entirety of the at least one magnetic core 14” means that at least 80% of each magnetic core 14 is covered by the coating 16. The coating 16 comprises a polymer 18 having at least one functional group 20. The at least one functional group 20 is selected from the group consisting of an amine (e.g., amino), a thiol (also referred to as a sulfanyl group or a sulfhydryl group), a hydroxyl, a carboxyl, a phosphate, an aldehyde, an azide, a vinyl (e.g., vinyl sulfone), an alkyne/dibenzocyclooctyne (DBCO), a lysine, a maleimide, biotin, and combinations thereof, as non-limiting examples. The at least one functional group 20 is a part of at least one of the backbone of the polymer 18, i.e., within the polymer 18 itself, or a cross-linking agent that cross-links the polymer 18. The at least one functional group 20 is configured to couple, i.e., conjugate, detectable agents to the at least one magnetic core 14. As used herein, “detectable agents” are compounds that provide contrast for imaging methods. The at least one functional group 20 can be used for “click chemistry” to conjugate at least one detectable agent to the at least one magnetic core 14. The polymer 18 can be dextran (having an average molecule weight of greater than or equal to about 1000 g/mol to less than or equal to about 100,000 g/mol; such as dextran T1, T1.5, G3.5, T5, T6, T10, T20, T25, T40, T60, T70, or T110), polyethylene glycol (PEG), poly ethyl methacrylate, poly methyl methacrylate, functionalized derivatives thereof, or combinations thereof, as non-limiting examples. When present, the cross-linker can be epichlorohydrin, N,N′-methylenebis(acrylamide), functionalized derivatives thereof, or combinations thereof, as non-limiting examples. In some aspects, the polymer 18 comprises dextran that is modified to have at least one functional group 20 or unmodified dextran that is cross-liked with a cross-linking agent comprising the at least one functional group 20, such as aminated, thioloated, or carboxylated epichlorohydrin.
As discussed above, the nanoparticle 10 comprises at least one magnetic core 14. The at least one magnetic core 14 is substantially spherical or semi-spherical. As used herein, “substantially spherical” means that the at least one magnetic core 14 may not be a perfect sphere and may include surface, i.e., topological, irregularities, such as crests, dips, flat portions, and the like.
In various aspects, and with reference to
In various other aspects, and with reference to
Referring back to
In some aspects, the nanoparticle 10 comprises the iodine chelator 22. The iodine chelator 22 is a compound that chelates iodine or otherwise binds iodine such that the iodine is detectable, i.e., provides contrast, for CT. The iodine chelator 22 binds to the at least one functional group 20 directly or indirectly by way of at least one linker. As non-limiting examples, the iodine chelator 22 can be T4, T3, or a combination thereof. Conventional iodine tracers can also be used as the iodine chelator 22. In certain aspects, the iodine chelator 22 is coupled to the at least one functional group 20 on the cross-linking agent by the linkers SMPH and SPDP. When present, the nanoparticle 10 provides contrast for MRI and MPI by way of the at least one magnetic core 14 and for X-ray imaging and CT by way of the iodine bound to the iodine chelator 22. In some aspects, the iodine chelator 22 is present on the nanoparticle 10 in greater than or equal to about 3 to less than or equal to about 20 or more copies or molecules.
In some aspects, the nanoparticle 10 comprises the fluorophore 24. The fluorophore 24 is a compound that luminesces when stimulated by certain wavelengths of light and provides contrast for at least one of FL imaging, PA imaging, or US imaging. As non-limiting examples, the fluorophore 24 can be a cyanine dye, a coumarin, a rhodamine, a xanthene (e.g., fluorescein and rhodamine), a quantum dot, derivatives thereof, and combinations thereof. Non-limiting examples of cyanine dyes include Cy3, Cy3.5, Cy5, Cy5.5, Cy7, Cy7.5, and combinations thereof. Derivatives of the fluorophore 24 include a base fluorophore compound that is modified to improve at least one of performance, for example, by minimizing photobleaching and pH-dependent fluorescent intensity, or coupling to the at least one functional group 20. The modified base fluorophore can be sulfated, isothiocyanated, modified with an N-hydroxysuccinimide (NHS) ester, modified with a maleimide, modified with biorthogonal conjugates, such as azide, azido, alkyne, tetrazine, or alkene, or otherwise modified to improve performance or coupling. Non-limiting examples of commercially available fluorophores include Alexa Fuor® fluorescent dyes commercialized by Thermo Fisher Scientific, Dylight™ fluorescent dyes, and Opal™ fluorescent dyes commercialized by PerkinElmer. When present, the nanoparticle 10 provides contrast for MRI and MPI by way of the at least one magnetic core 14 and for at least one of FL imaging, PA imaging, or US imaging by way of the fluorophore 24. In some aspects, the fluorophore 24 is present on the nanoparticle 10 in greater than or equal to about 1 to less than or equal to about 15 or more copies or molecules.
In some aspects, the nanoparticle 10 comprises the chelating compound that chelates 64Cu 26. The chelating compound that chelates 64Cu 26 is a compound that chelates 64Cu or that otherwise binds 64Cu such that the 64Cu is detectable, i.e., provides contrast, for PET. The chelating compound that chelates 64Cu 26 binds to the at least one functional group 20 directly or indirectly by way of at least one linker. As non-limiting examples, the chelating compound that chelates 64Cu 26 can be S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (p-SCN-Bn-DOTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester), 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA), or combinations thereof. When present, the nanoparticle 10 provides contrast for MRI and MPI by way of the at least one magnetic core 14 and for PET by way of the compound that chelates 64Cu 26.
In some aspects, the nanoparticle 10 comprises the chelating compound that chelates 99mTc 28. The chelating compound that chelates 99mTc 28 is a compound that chelates 99mTc or that otherwise binds 99mTc such that the 99mTc is detectable, i.e., provides contrast, for SPECT. The chelating compound that chelates 99mTc 28 binds to the at least one functional group 20 directly or indirectly by way of at least one linker. As a non-limiting example, the chelating compound that chelates 99mTc 28 can be [(R)-2-amino-3-(4-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-pentaacetic acid (SCN-Bn-CHx-DTPA). When present, the nanoparticle 10 provides contrast for MRI, MPI, and X-ray imaging by way of the at least one magnetic core 14 and for SPECT by way of the chelating compound that chelates 99mTc 28.
The nanoparticle 10 is configured as a multi-modal contrast agent because it includes the at least one magnetic core 14 (which provides contrast for MRI and MPI) and at least one of the iodine chelator 22 (which provides contrast for X-ray imaging and CT), the fluorophore 24 (which provides contrast for at least one of FL imaging, PA imaging, or US imaging), the chelating compound that chelates 64Cu 26 (which provides contrast for PET), or the chelating compound that chelates 99mTc 28 (which provides contrast for SPECT). When the nanoparticle 10 comprises each of the iodine chelator 22, the fluorophore 24, the chelating compound that chelates 64Cu 26, and the chelating compound that chelates 99mTc 28, a subject receiving a single administration of a composition comprising the nanoparticle 10 can sequentially undergo MRI, MPI, X-ray imaging, CT, at least one of FL imaging, PA imaging, or US imaging, PET, and SPECT, either during a single day or on different days within about 2 weeks without receiving a second administration of the composition or of another contrast agent. It is understood that the nanoparticle 10 can be used to provide contrast for MRI and MPI, along with at least one of X-ray imaging, CT, FL imaging, PA imaging, US imaging, PET, and SPECT, depending on the at least one detectable agent that is coupled to the at least one magnetic core 14 of the nanoparticle 10.
In some aspects, the nanoparticle also optionally includes an adjunct agent coupled to the at least one magnetic core 14. As non-limiting examples, the adjunct agent can be an oligonucleotide 30, a protein or peptide 32, an active pharmaceutical ingredient 34, or combinations thereof.
The current technology also provides a composition comprising the nanoparticle 10. The composition further comprises a pharmaceutically acceptable carrier suitable for intravenous, intraductal, intrathecal, intramuscular, intratumorally, or oral administration. Thus, the composition can be a liquid, a gel, or a solid, such as a tablet or capsule. The pharmaceutically acceptable carrier can include water, a saline solution, a preservative, a binder, or combinations thereof, as non-limiting examples.
The current technology also provides a method of making the nanoparticle 10. The method comprises forming the magnetic cores 14a described with reference to
The method of making the nanoparticle 10a comprising the plurality of magnetic cores 14a having a polymeric coating comprises dissolving a polymer in water to a polymer concentration of greater than or equal to about 15% to less than or equal to about 40%, optionally in an ice bath, to form a polymer solution. Next, the method comprises flowing an inert gas through the polymer solution, such as nitrogen, neon, or argon, as non-limiting examples, and adding iron(III) chloride hexahydrate (FeCl3.6H2O) to a final concentration of greater than or equal to about 0.5% to less than or equal to about 5%. Next, the method comprises adding iron(II) chloride hexahydrate (FeCl2.4H2O) to a final concentration of greater than or equal to about 0.25% to less than or equal to about 2.5%. Next, the method comprises adding NH4OH to a final concentration of greater than or equal to about 20% to less than or equal to about 40% and increasing the temperature to greater than or equal to about 70° C. to less than or equal to about 90° C. for greater than or equal to about 5 minutes to less than or equal to about 2 hours or longer. The method then comprises concentrating the solution to a final volume that is about 60% of the previous volume and adding NaOH to a final concentration of greater than or equal to about 1 M to less than or equal to about 3 M and greater than or equal to about 10% to less than or equal to about 25% of concentrated (e.g., 90-99%) cross-linker and stirring for greater than or equal to about 1 hour to less than or equal to about 24 hours. The cross-linker can be aminated as described below.
In some aspects, the method comprises mixing about 18 g of Dextran-T10 with about 60 mL of double-distilled water and stirring in a round bottom flask in an ice bath until dissolved completely and a clear dextran suspension is formed. Then, the method comprises adding 1.3 g of FeCl3.6H2O into the clear dextran suspension while flushing argon gas into the reaction mixture. Next, the method comprises dissolving 0.8 g of FeCl2.4H2O in 5 mL of distilled water, which was previously flushed with argon gas in order to avoid oxidation, and adding this solution into the clear dextran suspension to form a reaction mixture. The method then comprises adding about 30 mL of concentrated cold NH4OH (approximately 28%) to the reaction mixture and increasing the temperature to from about 75° C. to about 85° C. for about one hour. Next, the method comprises cooling to room temperature and concentrating to about 40 mL using centrifugal units (MWCO 100 kDa).
The method of making the nanoparticle 10b comprising the single magnetic core 14b comprises combining a magnetic compound source, such as iron(II) chloride hexahydrate as a non-limiting example, with water to form a solution comprising greater than or equal to about 0.5% to less than or equal to about 10% of the iron magnetic compound source. The resulting solution is stirred at about atmospheric temperature and pressure for greater than or equal to about 0.5 minutes to less than or equal to about 20 minutes to allow for oxygen equilibrium. The method then comprises introducing an inert gas, such as nitrogen, neon, or argon, as non-limiting examples, to the oxygen-equilibrated solution for greater than or equal to about 1 minute to less than or equal to about 60 minutes. A base, such as NaOH or KOH, is then added to a final concentration of greater than or equal to about 0.5 M to less than or equal to about 1 M, and the reaction is maintained for greater than or equal to about 1 minute to less than or equal to about 30 minutes. Thus, the method comprises forming the magnetic nanoparticles by reacting a magnetic compound source with a base in water under the flow of an inert gas to form a reaction mixture.
Next, the method comprises introducing a first addition of H2O2 to the reaction mixture to a final concentration of greater than or equal to about 0.005% to less than or equal to about 0.1% and allowing the solution to react for greater than or equal to about 0.5 minutes to less than or equal to about 20 minutes. A second addition of H2O2 is then introduced to the reaction mixture to the same final concentration, and the reaction mixture is again allowed to react for greater than or equal to about 0.5 minutes to less than or equal to about 20 minutes to complete the formation of the magnetic nanoparticles, which can be separated from the reaction mixture with a magnet.
The method also comprises disposing the coating 16 on the at least one magnetic core 14. Here, the method comprises suspending the at least one magnetic core 14 in water, adding a polymer to the suspension to a final concentration of greater than or equal to about 2% to less than or equal to about 10%, and stirring the suspension for greater than or equal to about 1 minute to less than or equal to about 60 minutes. The suspension is then brought to a temperature of greater than or equal to about 50° C. to less than or equal to about 100° C. under the flow of an inert gas and maintained for greater than or equal to about 1 hour to less than or equal to about 24 hours. Then, the method comprises bringing the suspension to ambient or room temperature, magnetically separating the at least one magnetic core 14 to concentrate the suspension, and filtering the suspension to remove excess polymer and to form the polymer 18 of the coating 16.
When necessary, the method comprises cross-linking the polymer 18 with the cross-linking agent comprising the at least one functional group 20. Here, the method comprises adding a base, e.g., NaOH or KOH, to the suspension comprising the at least one magnetic core 14 at least partially coated with the polymer 18 to a final concentration of greater than or equal to about 0.1 M to less than or equal to about 1 M and mixing for greater than or equal to about 1 minute to less than or equal to about 1 hour. Next, the method comprises adding a cross-linking agent to the suspension and mixing for greater than or equal to about 1 hour to less than or equal to about 24 hours to form the coating 16 comprising the polymer 18 and the cross-linking agent on a least a portion of the at least one magnetic core 14.
The polymer 18 or the cross-linking agent, when present, can be modified to include the at least one functional group 20. The following description is based on aminating the cross-linking agent to result in a plurality of aminated cross-linking molecules. However, other modifications can be made using methods known in the art.
Aminating is performed by adding concentrated NH4OH, e.g., from about 20% to about 40% NH4OH, to the suspension to a final NH4OH concentration of from about 1% to about 15% and stirring for greater than or equal to about 1 hour to less than or equal to about 24 hours. The suspension is then membrane purified, i.e., dialyzed, to remove small molecules, and the at least one magnetic core 14 is concentrated by magnetic separation.
In certain aspects, the polymer 18 comprises dextran, and the cross-linking agent comprises aminated epichlorohydrin.
The method further comprises conjugating a first portion of the aminated cross-linking agent molecules, or other molecules of the polymer 18 that include the at least one functional group 20, with a detectable agent.
In some aspects, the method comprises conjugating a portion of the aminated epichlorohydrin molecules with the iodine chelator 22, such as by contacting the aminated cross-linking agent with SPDP to form cross-linking agent molecules comprising SPDP, contacting an iodine chelator with SMPH to form an iodine chelator comprising SMPH, and contacting the cross-linking agent molecules comprising SPDP with the iodine chelator comprising SMPH to complete the conjugation.
In some aspects, the method comprises conjugating a portion of the aminated epichlorohydrin molecules with the fluorophore 24, such as by contacting the aminated cross-linking agent with a fluorophore having an NHS moiety.
In some aspects, the method comprises conjugating a portion of the aminated epichlorohydrin molecules with the chelating compound that chelates 64Cu 26, such as by contacting the aminated cross-linking agent with a chelating compound that chelates 64Cu having an NHS moiety.
In some aspects, the method comprises conjugating a portion of the aminated epichlorohydrin molecules with the chelating compound that chelates 99mTc 28, such as by contacting the aminated cross-linking agent with a chelating compound that chelates 99mTc having an NHS moiety.
The current technology yet further provides a method of imaging at least one of a tissue or a metabolic process in a subject in need thereof. The method comprises administering to the subject a safe and effective amount of a composition comprising the nanoparticle 10 (or plurality of nanoparticles 10) discussed above, wherein the nanoparticle 10 is a multi-modal contrast agent. As used herein, the term “effective amount” means an amount of a compound which, when administered to a subject, is sufficient to provide contrast for each imaging method being performed on the subject. The “effective amount” will vary depending on, for example, the composition form (e.g., the carriers present), the nanoparticle form (i.e., the detectable agents present), the dosage form, the condition requiring imaging, the anatomical region requiring imaging, and the age and weight of the patient to be subjected to the imaging. In various aspects, the effective amount of the composition provides a dose of greater than or equal to about 1 mg/kg to less than or equal to about 100 mg/kg. As used herein, the “subject” is a human or non-human mammal, a bird, a reptile, or an amphibian. The administering can be performed intravenously, intraductally, intrathecally, intramuscularly, intratumorally, or orally.
Depending on the detectable agents present on the nanoparticle 10, the method further comprises performing at least one or at least two of MRI, MPI, FL imaging, PA imaging, US imaging, PET, SPECT, X-ray imaging, or CT on the subject and generating at least one or at least two images of the tissue or the metabolic process with contrast provided by the nanoparticle 10.
Embodiments of the present technology are further illustrated through the following non-limiting example.
A multi-modal magnetic nanoparticle contrast agent is prepared as follows.
Iron oxide nanoparticles are synthesized with a newly adapted co-precipitation technique. Briefly, 2.0 g of iron(II) chloride hexahydrate are dissolved in 100 mL of deionized (DI) water with stirring at atmospheric temperature and pressure for 5 minutes to allow for oxygen equilibrium. Afterwards, nitrogen gas flow is started and allowed to flow for 15 minutes before initiation of the reaction. 20 mL of a 5 M KOH solution is then added to the solution and mixed for 10 minutes under the nitrogen gas flow. To this solution, 250 μL of a 5% H2O2 in DI water is added twice, with 5 minutes between the additions. 5 minutes after the second addition of 5% H2O2 in DI, the solution is removed from nitrogen flow and capped to undergo magnetic separation. A potent magnetic field is applied for about 20 minutes to fully pull the nanoparticles out of solution and the supernatant is removed. The nanoparticles are resuspended in 70 mL of DI water and allowed to stir for 5 minutes.
After the stirring, 4 g of dextran T-10 is added to the solution and it is again allowed to stir for 15 minutes. This mixture of nanoparticles and dextran is then brought to 85° C. under nitrogen flow and kept there for 8 hours. After the 8 hour incubation, the particles are removed from the heat and brought to room temperature. They are again magnetically separated to concentrate the solution, followed by purification with Millipore® filter devices to remove excess dextran. 7 mL of nanoparticle solution is added at a time to the filter device, and it is spun at 3750 rpm for 15 minutes. This filtering is repeated until a total final volume of 20 mL of the dextran-coated nanoparticles is obtained.
The nanoparticles are then cross-linked with epichlorohydrin. First, 20 mL of a 1 M NaOH solution is added and mixed for 10 minutes. To this, 11 mL of 99% epichlorohydrin is added, and the solution is allowed to mix for 8 hours. This reaction yields nanoparticles at least partially coated with a coating comprising dextran and aminated epichlorohydrin.
Amination of the epichlorohydrin is carried out by adding 45 mL of 30% NH4OH and stirring a gently capped round bottom flask for about 36 hours. This solution is purified with a membrane against DI water to remove small molecules from the nanoparticles. These particles can be concentrated by magnetic separation and then be placed on a bath sonicator with constant agitation for about 2 to 3 days. Stopping of the sonication and resuspending the aggregates in solution is important for not losing nanoparticles. After sonication is complete, the nanoparticles are completely soluble in aqueous environments and characterization can be performed. The coated nanoparticles are determined to be 20-30 nm in diameter with a sub-5 nm dextran coating. At this point, the coated nanoparticles are also ready to undergo conjugation.
The coated nanoparticles are conjugated with a Cy7-NHS or Cy5.5-NHS dye molecule (Lumiprobe) to allow for FL/PA/US imaging. Briefly, 100 μL of the dye (10 mM stock) suspended in DMSO is mixed with 400 μL of a sodium citrate buffer (20 mM Na-Citrate, 150 mM NaCl, pH 8.4) to dissolve fully. Next, 1.1 mL of the coated nanoparticles are added to this mixture and placed on a rotor overnight at room temperature. After the conjugation is complete, removal of the excess dye is performed with a PD-10 column against a sodium citrate buffer. The number of dye molecules per nanoparticle is determined to be 11.
Similar to the fluorophore conjugation, a DOTA-NHS chelator is next conjugated to the coated nanoparticles to allow for PET imaging. Initially dissolving the chelator in DMSO allows for a similar reaction as previously described. Briefly, 100 μL of the DOTA-NHS (2 mM stock) is first mixed with 300 μL of a sodium citrate buffer (20 mM Na-Citrate, 150 mM NaCl, pH 8.4). To this, 1 mL of the fluorophore-labeled nanoparticles is added slowly, so as to not to induce cloudiness in the solution. The mixture is again placed on a rotor overnight at room temperature and purified with a PD-10 column against a PBS buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.2) when complete. DTPA is conjugated to the core by a similar method using SCN-Bn-CHx-DTPA instead of DOTA-NHS.
To allow for CT imaging of the nanoparticle constructs, the hormone T4 is conjugated. To obtain this, 100 μL of SPDP (100 mM stock) is first added to 300 μL of the sodium citrate buffer (20 mM Na-Citrate, 150 mM NaCl, pH 8.4). 1 mL of the fluorophore/DOTA-NHS-conjugated coated nanoparticles is then mixed slowly with this to avoid the formation of cloudiness and placed on a rotor at room temperature overnight. These nanoparticles are purified against a PBS buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.2) with a PD-10 column and, once purified, are reacted with 100 μL of TCEP (10 mM stock) to form a reactive thiol group when the T4-linker mixture is prepared. PD-10 column washing against the PBS buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.2) is again performed to remove the reactive small molecules from the magnetic nanoparticles. A reaction scheme for this aspect is shown in
Prior to coupling T4 to the nanoparticles, the nanoparticles are transferred to a microcentrifuge tube and subjected to MPI.
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are transferred to a microcentrifuge tube and subjected to MPI.
Tubes containing various nanoparticle samples are placed in a vessel containing water and subjected to MRI. The samples include nanoparticles conjugated with fluorophore, DOTA, and NOTA (A), nanoparticles conjugated with fluorophore (B), nanoparticles conjugated with fluorophore and T4 (C), particles prepared by a different protocol (D), and water background (E).
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are transferred to a microcentrifuge tube and subjected to FL imaging in an in vivo imaging system (IVIS).
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are transferred to a microcentrifuge tube and subjected to CT.
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are intraductally administered to the mammary glands of a mouse. The mouse is then subjected to MPI and CT.
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are intraductally administered to the mammary glands of two mice. The mice are then subjected to FL imaging in an IVIS. Fluorescence images of the two mice are shown in
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are intraductally administered to the mammary glands of a mouse. The mouse is then subjected to CT.
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA, and T4 are intravenously administered to a mouse having a tumor induced by the administration of human breast adenocarcinoma cells (MDA-MB-231). The mouse is then subjected to FL imaging in an IVIS.
Nanoparticles conjugated with the fluorophore, DOTA-NHS, DTPA and T4 are intraductally administered to the mammary glands of a mouse. The mouse is then subjected to PET/MRI.
The foregoing description of the embodiments has been provided for purposes of illustration and description. It is not intended to be exhaustive or to limit the disclosure. Individual elements or features of a particular embodiment are generally not limited to that particular embodiment, but, where applicable, are interchangeable and can be used in a selected embodiment, even if not specifically shown or described. The same may also be varied in many ways. Such variations are not to be regarded as a departure from the disclosure, and all such modifications are intended to be included within the scope of the disclosure.
This application claims the benefit of U.S. Provisional Application 63/058,225, filed Jul. 29, 2020. The entire disclosure of the above application is incorporated herein by reference.
Number | Date | Country | |
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63058225 | Jul 2020 | US |