Claims
- 1. A solvent permeated gel for a microfabricated device comprising
an entangled or crosslinked polymeric network; and an excess of a solvent permeating said polymeric network, wherein said solvent permeated gel has sufficient dimensional rigidity and stability to maintain a specific shaped compartment microfabricated into said gel and wherein said excess of solvent permeating said polymeric network is sufficient to reduce or eliminate evaporation of liquid from any said compartment.
- 2. The solvent permeated gel of claim 1, wherein said polymeric network comprises agarose or polyacrylamide.
- 3. The solvent permeated gel of claim 1, wherein said polymeric network is a composite of polymers having different pore sizes.
- 4. The solvent permeated gel of claim 1, wherein said solvent comprises water.
- 5. The solvent permeated gel of claim 1, wherein said solvent comprises an organic solvent or a mixed aqueous/organic solvent.
- 6. A microfabricated device comprising
a solvent permeated gel, said gel comprising an entangled or crosslinked polymeric network and an excess of a solvent permeating said polymeric network, wherein a plurality of microscale compartments are fabricated in said solvent permeated gel, said gel further has sufficient dimensional rigidity and stability to maintain the shape of said compartments and said excess of solvent permeating said polymeric network is sufficient to reduce or eliminate evaporation of liquid from said compartments.
- 7. The microfabricated device of claim 6, wherein said solvent permeated gel comprises immobilized buffer components and said device is configured for micropreparative isoelectric focusing.
- 8. The microfabricated device of claim 6, wherein a pH gradient is established in said gel across said device.
- 9. The micro fabricated device of claim 6, wherein individual said compartments comprise an antibody or an antigen immobilized in said compartment.
- 10. The microfabricated device of claim 6, wherein individual said compartments comprise an enzyme immobilized in said compartment.
- 11. The microfabricated device of claim 6, wherein individual said compartments comprise nucleic acid immobilized in said compartment.
- 12. The microfabricated device of claim 6, wherein individual said compartments comprise inert particles to which active elements can be attached immobilized in said compartment.
- 13. The microfabricated device of claim 6, wherein said particles are magnetic beads.
- 14. The microfabricated device of claim 6, wherein individual said compartments in said device are configured as channels.
- 15. The microfabricated device of claim 6, wherein individual said microscale compartments in said device are configured as wells.
- 16. The microfabricated device of claim 6, wherein individual said microscale compartments in said device are configured as nozzle-shaped wells.
- 17. The microfabricated device of claim 6, wherein said polymeric network comprises agarose or polyacrylamide.
- 18. The microfabricated device of claim 6, wherein said polymeric network is a composite of polymers having different pore sizes.
- 19. The microfabricated device of claim 6, wherein said solvent comprises water.
- 20. The microfabricated device of claim 6, wherein said solvent comprises an organic solvent or a mixed aqueous/organic solvent.
- 21. A microfabricated device comprising
a solvent permeated gel, said gel consisting essentially of an entangled or crosslinked polymeric network and an excess of a solvent permeating said polymeric network, wherein a plurality of microscale compartments are fabricated in said solvent permeated gel, said gel further has sufficient dimensional rigidity and stability to maintain the shape of said compartments and said excess of solvent permeating said polymeric network is sufficient to reduce or eliminate evaporation of liquid from said compartments.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. application Ser. No. 09/530,118 filed Apr. 24, 2000, entitled A MULTICHANNEL MICROSCALE SYSTEM FOR HIGH THROUGHPUT PREPARATIVE SEPARATION WITH COMPREHENSIVE COLLECTION AND ANALYSIS which claims priority from U.S. Provisional Patent Application No. 60/062787, filed Oct. 24, 1997, the whole of which are hereby incorporated by reference herein.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
[0002] Part of the work leading to this invention was carried out with United States Government support provided under a grant from the Department of Energy, Grant No. DE-FG02-90ER60985. Therefore, the U.S. Government has certain rights in this invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60062787 |
Oct 1997 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09530118 |
Apr 2000 |
US |
Child |
10653398 |
Sep 2003 |
US |