Claims
- 1. A particulate label which comprises a particulate support to which is bound at least two signal-generating moieties in a predetermined amount and ratio, each of which moieties generates, in situ, a signal different from that generated by the other(s) and wherein each of said signals can be varied over a series of gradations.
- 2. The label of claim 1 wherein said particulate support is coupled to at least three said signal-generating moieties.
- 3. The label of claim 1 wherein said signal-generating moieties are color generating moieties.
- 4. The label of claim 3 wherein the color generating moieties are fluorophores.
- 5. The label of claim 1 wherein the particulate support is a latex bead.
- 6. The label of claim 1 which is further associated with a reagent.
- 7. The label of claim 6 wherein the reagent is selected from the group consisting of an antibody;
a peptide generated from a cDNA library; a substance in a combinatorial chemistry library; and an oligonucleotide.
- 8. The label of claim 6 wherein said reagent is covalently linked to the label.
- 9. The label of claim 8 wherein the covalent linkage is a disulfide or carboxamide linkage.
- 10. The label of claim 6 wherein the reagent is noncovalently linked to the label.
- 11. The label of claim 10 wherein the noncovalent linkage is an epitope/antibody linkage or a histidine/Ni-chelator linkage or comprises an agar layer.
- 12. A collection of the particulate labels of claim 1 wherein said collection comprises multiplicities of said labels having different ratios and/or amounts of said signal-generating moieties so each label has a different hue.
- 13. A collection of the particulate labels of claim 6 wherein said collection comprises multiplicities of said labels having different ratios and/or amounts of said signal-generating moieties so each label has a different hue, and wherein each label of a given hue is associated with a different known reagent.
- 14. The collection of claim 12 wherein said signal-generating moieties are color generating moieties.
- 15. The collection of claim 14 wherein the color generating moieties are fluorophores.
- 16. The collection of claim 13 wherein said signal-generating moieties are color generating moieties.
- 17. The collection of claim 12 wherein the labels of different hues are arranged in a matrix.
- 18. A method to assess a sample for its constituent components which method comprises contacting said sample with the collection of claim 13 and determining which labels are bound and which labels are not bound by said sample.
- 19. A system for the detection of an interaction between a multiplicity of reagents and at least one target which system comprises the collection of particulate labels of claim 16 along with a detecting means for each different wavelength band of each color generating moiety.
- 20. The system of claim 19 wherein said particulate labels are coupled to a competing analog of said target contained in a position wherein the labels can be detected, and which further includes a means for flowing sample containing said target past said labeled analog whereby any target in the sample competes with the analog for the label.
- 21. The system of claim 20 wherein said target is a drug or a metabolite of a drug.
- 22. A method to follow the course of a network of physiological pathways which method comprises
contacting a series of end points of said pathways with the matrix of claim 17; and observing the progress of the labels from each position in the matrix.
- 23. A method to assess the interaction of a first library of compounds with a second library containing, potentially, compounds which are opposite members of specific binding pairs with compounds contained in said first library which method comprises
providing coupled members of said first library to the particulate labels of claim 1 so that each member of the library is coupled to a label with a signal different from the other members of said library; providing coupled members of the second library to the labels of claim 1 so that each member of said second library is coupled to a label with a signal different from the other members of said library; wherein the labels in said first library are substantially larger in size than the labels of said second library; contacting the first labeled library with the second labeled library; and observing the formation of rosettes, thus identifying members of the first library which interact with members of the second library.
- 24. The method of claim 23 which further includes the step of recording, for each interaction, the formation or nonformation of a rosette and storing said recorded data in computer-readable form.
- 25. The recorded data of claim 24 in said computer-readable form.
- 26. The method of claim 23 wherein the members of the first library are proteins and the members of the second library are small molecules sharing the same scaffold.
- 27. The method of claim 23 wherein the members of the first library are antigens and the members of the second library are antibodies or fragments thereof.
- 28. The method defined in claim 23, wherein the members of the first library are small molecules and the members of the second library are receptors.
- 29. The method defined in claim 23, wherein the members of the first library are potential ligands and the members of the second library are receptors.
Parent Case Info
[0001] This application is a continuation-in-part of U.S. Ser. No. 09/146,984 filed Sep. 3, 1998 and incorporated herein by reference.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09146984 |
Sep 1998 |
US |
Child |
09332613 |
Jun 1999 |
US |