Claims
- 1. A multiple DNA vaccine for in ovo injection comprising:
two or more DNA constructs, each DNA construct expressing an antigenic protein of an avian virus causing avian viral disease in fowl; wherein said antigenic protein of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl; and said multiple DNA vaccine is injected into egg of said fowl.
- 2. The multiple DNA vaccine according to claim 1, wherein said avian virus is one selected from the group consisting of Marek's disease virus (MDV), infectious vursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian encephalomyelitis (AEV), avian leukosis virus (ALV), fowlpox virus (FPV), avian parainfluenza virus (APV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).
- 3. The multiple DNA vaccine according to claim 1, wherein said DNA construct comprises a DNA molecule encoding said antigenic protein of said avian virus and a vector.
- 4. The multiple DNA vaccine according to claim 1, wherein said vector is a plasmid or a viral carrier.
- 5. The multiple DNA vaccine according to claim 4, wherein said plasmid is one selected from the group consisting of pcDNA3, pVAX1, pSectag, pTracer, pDisplay, pUC system plasmid, and pGEM system plasmid.
- 6. The multiple DNA vaccine according to claim 4, wherein said plasmid comprises a promoter which is selected from the group consisting of a CMV promoter, SV40 promoter, RSV promoter, and β-actin promoter.
- 7. The multiple DNA vaccine according to claim 4, wherein said viral carrier is one selected from the group consisting of a baculovirus, a herpes virus, and a pox virus.
- 8. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire of gB gene of Marek's Disease virus (MDV) having the DNA sequence of SEQ ID NO:1 or a fragment thereof.
- 9. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire VP2 gene of infectious bursal disease virus (IBDV) having the DNA sequence of SEQ ID NO:2 or a fragment thereof.
- 10. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire HN gene of Newcastle disease virus (NDV) having the DNA sequence of bases 6321 to 8319 in SEQ ID NO:3 or a fragment thereof.
- 11. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire S1 gene of infectious bronchitis virus (IBV) having the DNA sequence of SEQ ID NO:4 or a fragment thereof.
- 12. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire glycoprotein G gene of infectious laryngotracheitis virus (ILTV) having the DNA sequence of SEQ ID NO:5 or a fragment thereof.
- 13. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire VP1, VP0, or VP3 gene of avian encephalomyelitis virus (AEV) or a fragment therof; wherein said VP1 gene has the DNA sequence of SEQ ID NO:6; said VPO gene has the DNA sequence of SEQ ID NO:7; and said VP3 gene has the DNA sequence of SEQ ID NO:8.
- 14. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire paraglycoprotein G gene of avian parainfluenza virus (APV) having the DNA sequence of SEQ ID NO:9 or a fragment thereof.
- 15. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire type A penton base gene of hemorrhagic enteritis virus (HEV) having the DNA sequence of SEQ ID NO:10 or a fragment thereof.
- 16. The multiple DNA vaccine according to claim 3, wherein said DNA molecule is an entire envelope antigen gene of fowlpox virus (FPV) having the DNA sequence of SEQ ID NO:11 or a fragment thereof.
- 17. The multiple DNA vaccine according to claim 1, wherein said multiple DNA vaccine is injected into an amniotic fluid of said egg.
- 18. The multiple DNA vaccine according to claim 17, wherein said egg is fertilized for about 18 days.
- 19. The multiple DNA vaccine according to claim 1, wherein said DNA vaccine comprises two DNA constructs, each containing a DNA molecule capable of expressing a gene or a fragment thereof which is selected from the group consisting of Marek's disease virus (MDV), infectious vursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian encephalomyelitis (AEV), avian leukosis virus (ALV), fowlpox virus (FPV), avian parainfluenza virus (APV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).
- 20. The multiple DNA vaccine according to claim 1, wherein said DNA vaccine comprises three or more DNA constructs, each containing a DNA molecule capable of expressing a gene or a fragment thereof which is selected from the group consisting of Marek's disease virus (MDV), infectious vursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian encephalomyelitis (AEV), avian leukosis virus (ALV), fowlpox virus (FPV), avian parainfluenza virus (APV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).
- 21. The multiple DNA vaccine according to claim 1, wherein said fowl is one selected from the group consisting of chicken, turkey, duck, and goose.
- 22. A method for vaccinating fowl egg comprising:
injecting into said fowl egg said multiple DNA vaccine according to claim 1.
- 23. A method for preparing said multiple DNA vaccine according to claim 1 comprising:
ligating a DNA molecule to a plasmid or virus carrier to form a DNA construct; mixing two or more said DNA constructs to form said multiple DNA vaccine; wherein said DNA molecule comprises a gene or a fragment thereof which is selected from the group consisting of Marek's disease virus (MDV), infectious vursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian encephalomyelitis (AEV), avian leukosis virus (ALV), fowlpox virus (FPV), avian parainfluenza virus (APV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).
- 24. A multivalent DNA vaccine for in ovo injection comprising:
a DNA construct containing two or more DNA molecules, wherein each of said DNA molecules express an antigenic protein of an avian virus causing avian viral disease in fowl; wherein said antigenic protein of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl; and said multivalent DNA vaccine is injected into egg of said fowl.
- 25. The multivalent DNA vaccine according to claim 22, wherein said DNA molecules is a gene or a fragment thereof which is selected from the group consisting of Marek's disease virus (MDV), infectious vursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian encephalomyelitis (AEV), avian leukosis virus (ALV), fowlpox virus (FPV), avian parainfluenza virus (APV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).
- 26. The multivalent DNA vaccine according to claim 24, wherein said DNA construct further comprises a plasmid or a virus carrier.
- 27. The multivalent DNA vaccine according to claim 26, wherein said plasmid is one selected from the group consisting of pcDNA3, pVAX1, pSectag, pTracer, pDisplay, pUC system plasmid, and pGEM system plasmid.
- 28. The multiple DNA vaccine according to claim 26, wherein said plasmid comprises a promoter which is selected from the group consisting of a CMV promoter, SV40 promoter, RSV promoter, and β-actin promoter.
- 29. The multiple DNA vaccine according to claim 26, wherein said virus carrier is one selected from the group consisting of a baculovirus, a herpes virus, and a pox virus.
- 30. A method for vaccinating fowl egg comprising:
injecting into said fowl egg said multivalent DNA vaccine according to claim 24.
RELATED APPLICATION
[0001] This application claims the priority of U.S. Provisional Application Serial No. 60/362,547, filed on Mar. 8, 2002, which is herein incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60362547 |
Mar 2002 |
US |