Claims
- 1. A process for building an immobilized structure comprising multiple monolayers of sequential polymeric linkages, said process comprising the steps of:
- (a) providing a solid phase with a first attached reactive moiety;
- (b) conducting a first coupling reaction by reacting said attached reactive moiety with an excess of carbonyldiimidazole as a first multifunctional reagent to produce an intermediate adduct attached to said solid phase which comprises a residue from said first reagent, and which provides a second reactive moiety capable of coupling to the second multifunctional reagent;
- (c) conducting a second coupling reaction by reacting said intermediate adduct with an excess of phenylenediamine as a second multifunctional reagent to produce a first monolayer of a phenylurethane molecular unit as said polymer linkage; said molecular unit providing a terminal reactive moiety capable of coupling to said first multifunctional reagent:
- (d) repeating said first coupling and second coupling reactions alternatively to produce additional monolayers of said phenylurethane molecular unit to obtain said immobilized structure comprising multiple monolayers of sequential polymeric linkage.
- 2. The process of claim 1 wherein said solid phase contains an amino functionality.
- 3. A process for immobilizing an enzyme comprising the steps of:
- (a) providing a solid phase with an attached amino functionality;
- (b) conducting a first coupling reaction by reacting said attached amino functionality with an excess of carbonyldiimidazole to produce an intermediate adduct which comprises a carbonyl-containing residue derived from said carbonyldiimidazole, and a reactive moiety capable of coupling to a second multifunctional reagent containing at least one amino functionality;
- (c) conducting a second coupling reaction by reacting said intermediate adduct with an excess of phenylenediamine as said second multifunctional reagent to produce a first monolayer of a phenylurethane molecular unit;
- (d) repeating said first coupling and second coupling reaction to produce at least four monolayers of phenylurethane linkages and a terminal amino functionality;
- (e) reacting said terminal amino functionality with carbonyldiimidazole to produce a terminal intermediate adduct capable of coupling to an enzyme;
- (f) reacting said resulting intermediate adduct with an enzyme to produce an enzyme immobilized onto a multiple monolayer structure.
- 4. The process of claim 3 wherein the enzyme is acetylcholinesterase.
- 5. A multiple monolayer structure produced according to the process of claim 1.
- 6. An immobilized enzyme produced according to the process of claim 12.
Parent Case Info
This application is a continuation, of application Ser. No. 16,337, filed 10/07/86 now abandoned.
US Referenced Citations (5)
Non-Patent Literature Citations (2)
Entry |
R. B. Merrifield, "Solid-Phase Peptide Synthesis", Biochemistry, vol. 3, No. 9, Sep., 1964. |
J. Am. Chem. Soc. 1983, 105, pp. 674-676, A New Approach to Construction of Artificial Monolayer Assemblies, L. Netzer and J. Sagiv. |
Continuations (1)
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Number |
Date |
Country |
Parent |
916337 |
Oct 1986 |
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