Claims
- 1. A system for measuring at least one signal from a waveguide sensor, the system comprising:
- a waveguide sensor having at least one wave propagating surface including a reactant coating immobilized thereon, the reactant coating including a first molecular species and a labeled molecular species, the first molecular species being bound to the waveguide sensor, the first molecular species being bound to the labeled molecular species, and the labeled molecular species having an excitation wavelength and an emission wavelength;
- a fluorescent reference material attached to the wave guide, the fluorescent reference material having an excitation wavelength and an emission wavelength;
- a source capable of emitting electromagnetic radiation at the excitation wavelength of the fluorescent reference material;
- a first detector capable of detecting the emission wavelength of the fluorescent reference material; and
- a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector.
- 2. A system in accordance with claim 1, further comprising a first dichroic element and a first filter, the first dichroic element being disposed between the source and the waveguide sensor along the first path, the first filter being disposed between the source and the first dichroic element along the first path, the first filter being capable of transmitting electromagnetic radiation at the excitation wavelength of the labeled molecular species and the excitation wavelength of the fluorescent reference material.
- 3. A system in accordance with claim 2, further comprising a second dichroic element and a second filter, the second dichroic element being disposed between the waveguide sensor and the first detector along the first path, the second filter being disposed between the second dichroic element and the first detector along the first path, the second filter being capable of filtering electromagnetic radiation at the emission wavelength of the labeled molecular species.
- 4. A system in accordance with claim 3, further comprising a second detector capable of detecting the emission wavelength of the labeled molecular species, wherein the coupler is capable of transmitting electromagnetic radiation along a second path from the source to the waveguide sensor to the second detector, and wherein the source is capable of emitting electromagnetic radiation at the excitation wavelength of the labeled molecular species.
- 5. A system in accordance with claim 4, further comprising a third filter disposed between the second dichroic element and the second detector along the second path, the third filter being capable of filtering electromagnetic radiation at the emission wavelength of the fluorescent reference material.
- 6. A system in accordance with claim 1, further comprising a second detector capable of detecting the emission wavelength of the labeled molecular species, wherein the coupler is capable of transmitting electromagnetic radiation along a second path from the source to the waveguide sensor to the second detector, and wherein the source is capable of emitting electromagnetic radiation at the excitation wavelength of the labeled molecular species.
- 7. A system in accordance with claim 6, further comprising a third filter disposed between the second dichroic element and the second detector along the second path, the third filter being capable of filtering electromagnetic radiation at the emission wavelength of the fluorescent reference material.
- 8. A system in accordance with claim 1, wherein the labeled molecular species is selected from the group consisting of antigens, antibodies, amino acid sequences, nucleic acid sequences and mixtures thereof.
- 9. A system in accordance with claim 1, wherein the first molecular species is selected from the group consisting of antigens, antibodies, amino acid sequences, nucleic acid sequences and mixtures thereof.
- 10. A system for measuring at least one signal from an evanescent waveguide sensor, the system comprising:
- a waveguide sensor capable of emitting first and second wavelengths;
- a fluorescent reference material attached to the waveguide sensor, the fluorescent reference material having an emission wavelength approximately equal to the first wavelength;
- a source capable of emitting electromagnetic radiation at the first and second wavelengths;
- a first detector capable of detecting electromagnetic radiation at the first wavelength;
- a second detector capable of detecting electromagnetic radiation at the second wavelength; and
- a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector and along a second path from the source to the waveguide sensor to the second detector.
- 11. A system in accordance with claim 10, further comprising a first dichroic element and a first filter, the first dichroic element being disposed between the source and the waveguide sensor along the first and second paths, the first filter being disposed between the source and the first dichroic .element along the first path, the first filter being capable of transmitting electromagnetic radiation at the first and second wavelengths.
- 12. A system in accordance with claim 11, further comprising a second dichroic element and a second filter, the second dichroic element being disposed between the waveguide sensor and the first detector along the first path, the second filter being disposed between the second dichroic element and the first detector along the first path, the second filter being capable of filtering electromagnetic radiation at the second wavelength.
- 13. A system in accordance with claim 12, further comprising a third filter disposed between the second dichroic element and the second detector along the second path, the third filter being capable of filtering electromagnetic radiation at the first wavelength.
- 14. A system in accordance with claim 13, further comprising a first molecular species and a labeled molecular species capable of binding to the analyte, the labeled molecular species having an emission wavelength approximately equal to the second wavelength.
- 15. A system in accordance with claim 14, wherein the labeled molecular species is selected from the group consisting of labeled antigens, labeled antibodies, labeled amino acid sequences, labeled nucleic acid sequences and mixtures thereof.
- 16. A system in accordance with claim 10, further comprising a first molecular species and a labeled molecular species, the labeled molecular species having an emission wavelength approximately equal to the second wavelength.
- 17. A system in accordance with claim 16, wherein the labeled molecular species is selected from the group consisting of labeled antigens, labeled antibodies, labeled amino acid sequences, labeled nucleic acid sequences and mixtures thereof.
- 18. A system for measuring at least one signal from a waveguide sensor, the system comprising:
- a waveguide sensor having at least one wave propagating surface including a reactant coating mobilized thereon, the reactant coating including a first molecular species and a labeling molecular species, the first molecular species being bound to the waveguide sensor and the labeling molecular species having an excitation wavelength and an emission wavelength, the labeling molecular species being bound to the first molecular species;
- a fluorescent reference material attached to the wave guide, the fluorescent reference material having an excitation wavelength and an emission wavelength;
- a source capable of emitting electromagnetic radiation at the excitation wavelength of the fluorescent reference material;
- a first detector capable of detecting the emission wavelength of the fluorescent reference material; and
- a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector.
- 19. A system in accordance with claim 18, further comprising a first dichroic element and a first filter, the first dichroic element being disposed between the source and the waveguide sensor along the first path, the first filter being disposed between the source and the first dichroic element along the first path, the first filter being capable of transmitting electromagnetic radiation at the excitation wavelength of the labeling molecular species and the excitation wavelength of the fluorescent reference material.
- 20. A system in accordance with claim 19, further comprising a second dichroic element and a second filter, the second dichroic element being disposed between the waveguide sensor and the first detector along the first path, the second filter being disposed between the second dichroic element and the first detector along the first path, the second filter being capable of filtering electromagnetic radiation at the emission wavelength of the labeling molecular species.
- 21. A system in accordance with claim 20, further comprising a second detector capable of detecting the emission wavelength of the labeling molecular species, wherein the coupler is capable of transmitting electromagnetic radiation along a second path from the source to the waveguide sensor to the second detector, and wherein the source is capable of emitting electromagnetic radiation at the excitation wavelength of the labeling molecular species.
- 22. A system in accordance with claim 21, further comprising a third filter disposed between the second dichroic element and the second detector along the second path, the third filter being capable of filtering electromagnetic radiation at the emission wavelength of the fluorescent reference material.
- 23. A system in accordance with claim 18, further comprising a second detector capable of detecting the emission wavelength of the labeling molecular species, wherein the coupler is capable of transmitting electromagnetic radiation along a second path from the source to the waveguide sensor to the second detector, and wherein the source is capable of emitting electromagnetic radiation at the excitation wavelength of the labeling molecular species.
- 24. A system in accordance with claim 23, further comprising a third filter disposed between the second dichroic element and the second detector along the second path, the third filter being capable of filtering electromagnetic radiation at the emission wavelength of the fluorescent reference material.
- 25. A system in accordance with claim 18, wherein the labeling molecular species is selected from the group consisting of labeled antigens, labeled antibodies, labeled amino acid sequences, labeled nucleic acid sequences and mixtures thereof.
- 26. A system in accordance with claim 18, wherein the first molecular species is selected from the group consisting of antigens, antibodies, amino acid sequences, nucleic acid sequences and mixtures thereof.
CROSS-REFERENCE TO RELATED APPLICATIONS
The present application is a divisional application of U.S. patent application Ser. No. 08/246,391 filed May 20, 1994, now U.S. Pat. No. 5,525,466 which is a continuation application of U.S. patent application Ser. No. 07/712,304, filed Jun. 7, 1991, now abandoned.
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Divisions (1)
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Number |
Date |
Country |
Parent |
246391 |
May 1994 |
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Continuations (1)
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Number |
Date |
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Parent |
712304 |
Jun 1991 |
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