Claims
- 1. A protease variant comprising substitution of an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and wherein the protease variant is more effective in a low detergent concentration system than a precursor protease.
- 2. The protease variant of claim 1 wherein the protease is a subtilisin.
- 3. The protease variant according to claim 1 which is derived from a Bacillus subtilisin.
- 4. The protease variant according to claim 3 which is derived from Bacillus lentus subtilisin.
- 5. A DNA encoding a protease variant of claim 1.
- 6. An expression vector encoding the DNA of claim 5.
- 7. A host cell transformed with the expression vector of claim 6.
- 8. A cleaning composition comprising the protease variant of claim 1.
- 9. An animal feed comprising the protease variant of claim 1.
- 10. A composition for treating a textile comprising the protease variant of claim 1.
- 11. A protease variant comprising substitution of an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and wherein the protease variant is more effective in a high detergent concentration system than a precursor protease.
- 12. The protease variant of claim 11 wherein the protease is a subtilisin.
- 13. The protease variant according to claim 11 which is derived from a Bacillus subtilisin.
- 14. The protease variant according to claim 13 which is derived from Bacillus lentus subtilisin.
- 15. A DNA encoding a protease variant of claim 11.
- 16. An expression vector encoding the DNA of claim 15.
- 17. A host cell transformed with the expression vector of claim 16.
- 18. A cleaning composition comprising the protease variant of claim 11.
- 19. An animal feed comprising the protease variant of claim 11.
- 20. A composition for treating a textile comprising the protease variant of claim 11.
- 21. The protease variant of claim 1 wherein the high detergent concentration is greater than 2000 ppm in the wash water.
- 22. A method of producing a protease variant that is more effective in a low, medium and high detergent concentration system than a precursor protease comprising:
a) substituting an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more positive or less negative compared to the precursor protease; b) substituting an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more negative or less positive compared to the precursor protease; c) testing the variant to determine its effectiveness in a high, medium and low detergent concentration system compared to the precursor protease; and d) repeating steps a)-c) as necessary to produce a protease variant that is more effective in a low, medium and high detergent concentration system than a precursor protease.
- 23. A protease variant comprising substitution of an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and wherein the protease variant is more effective in a medium detergent concentration system than a precursor protease.
- 24. A protease variant comprising substitution of an amino acid at one or more residue positions wherein the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and wherein the protease variant is more effective in a medium detergent concentration system than a precursor protease.
RELATED APPLICATIONS
[0001] The present application is a continuation-in-part application of U.S. patent application Ser. No. 08/956,323, filed Oct. 23, 1998, U.S. patent application Ser. No. 08/956,564, filed Oct. 23, 1998, and U.S. patent application Ser. No. 08/956,324 filed Oct. 23, 1998, all of which are hereby incorporated herein in their entirety.
Continuations (1)
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Number |
Date |
Country |
Parent |
09177353 |
Oct 1998 |
US |
Child |
10423649 |
Apr 2003 |
US |
Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
08956323 |
Oct 1997 |
US |
Child |
09177353 |
Oct 1998 |
US |
Parent |
08956564 |
Oct 1997 |
US |
Child |
09177353 |
Oct 1998 |
US |
Parent |
08956324 |
Oct 1997 |
US |
Child |
09177353 |
Oct 1998 |
US |