Claims
- 1. A protease variant comprising amino acid substitutions positions corresponding to the amino acid positions 103, 232, 236 and 245 in the amino acid sequence of the Bacillus amyloliquefaciens subtilisin set forth in SEQ ID NO:3 and further comprising a substitution of one or more amino acids at amino acid positions selected from the group of positions corresponding to positions in the amino acid sequence of the Bacillus amyloliquefaciens subtilisin set forth in SEQ ID NO:3 consisting of 3, 12, 20, 27, 38, 43, 48, 55, 61, 62, 68, 76, 87, 89, 97, 98, 99, 101, 102, 104, 106, 109, 128, 130, 131, 133, 140, 147, 159, 166, 167, 173, 183, 184, 185, 192, 194, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 218, 224, 237, 244, 248, 251, 252, 255, 256, 257, 258, 259, 260, 261, 271, 272, and 275.
- 2. The protease variant according to claim 1, wherein the further substitution comprises a substitution of an amino acid at an amino acid position selected from the group of positions consisting of 68, 76, 104, 159, 212, 248 and 252.
- 3. The protease variant according to claim 1 which is derived from a Bacillus subtilisin.
- 4. The protease variant according to claim 3 which is derived from Bacillus lentus subtilisin.
- 5. A DNA encoding a protease variant of claim 1.
- 6. An expression vector encoding the DNA of claim 5.
- 7. A host cell transformed with the expression vector of claim 6.
- 8. A cleaning composition comprising the protease variant of claim 1.
- 9. An animal feed comprising the protease variant of claim 1.
- 10. A composition for treating a textile comprising the protease variant of claim 1.
RELATED APPLICATIONS
The present application is a continuation-in-part application of U.S. patent application Ser. No. 09/178,155, filed Oct. 23, 1998 now U.S. Pat. No. 6,312,936, which was a continuation-in-part of U.S. patent application Ser. No. 08/956,323, filed Oct. 23, 1997 now abandoned, U.S. patent application Ser. No. 08/956,564, filed Oct. 23, 1997 now abandoned, and U.S. patent application Ser. No. 08/956,324 filed Oct. 23, 1997 now abandoned, all of which are hereby incorporated herein in their entirety.
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Continuations (1)
|
Number |
Date |
Country |
| Parent |
09/178155 |
Oct 1998 |
US |
| Child |
10/033325 |
|
US |
Continuation in Parts (3)
|
Number |
Date |
Country |
| Parent |
08/956323 |
Oct 1997 |
US |
| Child |
09/178155 |
|
US |
| Parent |
08/956564 |
Oct 1997 |
US |
| Child |
08/956323 |
|
US |
| Parent |
08/956324 |
Oct 1997 |
US |
| Child |
08/956564 |
|
US |
Patent Grant
6815193
