Claims
- 1. A purified N-methyltransferase, the N-methyltransferase:
(a) being present in Limonium latifolium; (b) having an isoelectric point of about 5.15; and (c) migrating on SDS-PAGE at about 43 kilodaltons.
- 2. The purified N-methyltransferase of claim 1, wherein the N-methyltransferase comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 1-5.
- 3. A purified protein comprising the amino acid sequence of SEQ ID NO: 1.
- 4. A purified protein comprising the amino acid sequence of SEQ ID NO: 2.
- 5. A purified protein comprising the amino acid sequence of SEQ ID NO: 3
- 6. A purified protein comprising the amino acid sequence of SEQ ID NO: 4.
- 7. A purified protein comprising the amino acid sequence of SEQ ID NO: 5.
- 8. A purified antibody that specifically binds an N-methyltransferase present in Limonium latifolium.
- 9. A purified antibody that specifically binds a polypeptide consisting of an amino acid sequence selected from the group consisting of: SEQ ID NOs: 1-5.
- 10. The purified antibody of claim 9, wherein the amino acid sequence is SEQ ID NO: 1.
- 11. The purified antibody of claim 9, wherein the amino acid sequence is SEQ ID NO: 2.
- 12. The purified antibody of claim 9, wherein the amino acid sequence is SEQ ID NO: 3.
- 13. The purified antibody of claim 9, wherein the amino acid sequence is SEQ ID NO: 4.
- 14. The purified antibody of claim 9, wherein the amino acid sequence is SEQ ID NO: 5.
- 15. A cell into which has been introduced a purified N-methyltransferase, the N-methyltransferase:
(a) being present in Limonium latifolium; (b) having an isoelectric point of about 5.15; and (c) migrating on SDS-PAGE at about 43 kilodaltons.
- 16. The cell of claim 1, wherein the N-methyltransferase comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs: 1-5.
- 17. A cell into which has been introduced a purified protein, the protein comprising an amino acid sequence selected from the group consisting of: SEQ ID NOs: 1-5.
- 18. The cell of claim 17, wherein the amino acid sequence is SEQ ID NO: 1.
- 19. The cell of claim 17, wherein the amino acid sequence is SEQ ID NO: 2.
- 20. The cell of claim 17, wherein the amino acid sequence is SEQ ID NO: 3
- 21. The cell of claim 17, wherein the amino acid sequence is SEQ ID NO: 4.
- 22. The cell of claim 17, wherein the amino acid sequence is SEQ ID NO: 5.
- 23. The cell of claim 17, wherein the cell is a plant cell.
- 24. The cell of claim 23, wherein the plant cell is in a plant.
CROSS REFERENCE TO RELATED APPLICATION
[0001] The present application claims the priority of U.S. provisional patent application No. 60/286,162, filed Apr. 24, 2001, and entitled “N-Methyltransferase Involved In Beta-alanine Betaine Synthesis.”
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
[0002] The invention was made with U.S. government support under grant numbers NRICGP 2001-35318-10947 and HOS3807, both awarded by the U.S. Department of Agriculture. The U.S. government may have certain rights in the invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60286162 |
Apr 2001 |
US |