N-substituted lauramides, their preparation and compositions in which they are present

The present invention relates to novel lauric acid amide derivatives substituted by an alkylamino group.
The present invention further relates to the use of the compounds according to the invention in compositions for antiseptic or antimicrobial use, as disinfectants or preservatives, especially in the sectors of pharmacy, cosmetology or agri-foodstuffs.
A publication of Czechoslovak origin by Devinsky et al., Chem. Zvesti., 1983, 37(2), 263-271 describes lauric acid alkylaminoalkylamides of the formula ##STR2## in which R=H, CH.sub.3 or C.sub.2 H.sub.5 and n=2 to 12.
Furthermore, three Czechoslovak patents by Devinsky et al. describe the compounds (B) ##STR3## in which n=3-5 and 7-12, as corrosion inhibitors, dispersants and auxiliaries in the textile industry (Czechoslovak patent 220297, 1982-Chem. Abs. 105, 225810a), the compounds (C) ##STR4## in which n=2 and 4-12, as auxiliaries in the textile industry and as possessing a bacteriostatic activity, without however specifying any values (Czechoslovak patent 220298, 1982-Chem. Abs. 105, 225811b), and the compounds (D) ##STR5## in which n=2, 4 and 12, as disinfectants, dispersants and local anesthetics (Czechoslovak patent 223443, 1982-Chem. Abs. 105, 208483d).
It has now been found that, in formula (A) in which R is hydrogen, replacement of one of these hydrogens with a benzyl group and the other with an alkyl, or replacement of both hydrogens together with a 3-benzyl-1,5-pentylene group so as to form a 4-benzylpiperidino group with the nitrogen, gives novel N-benzylaminoalkyllauramides having an excellent bactericidal activity superior to that of the corresponding dialkylaminoalkyl derivatives, and a good fungicidal activity.
The present invention therefore relates to aminoalkyllauramides of the formula ##STR6## in which R.sub.1 is a benzyl group and R.sub.2 is an alkyl having from 1 to 4 carbon atoms, or R.sub.1 and R.sub.2 form a 4-benzylpiperidino group with the nitrogen atom to which they are bonded, and n is 2, 3, 4, 5 or 6, and to the salts of the compounds (I) with organic or mineral acids.
The compounds (I) in which n=2 or n=3 are preferred compounds.
The salts of the compounds of formula (I) according to the present invention include those with mineral or organic acids which permit suitable separation or crystallization of the compounds of formula (I), such as picric acid or oxalic acid, as well as those with mineral or organic acids which form salts acceptable in pharmaceutical, edible, cosmetic or disinfectant compositions, such as the hydrochloride, hydrobromide, sulfate, hydrogensulfate, dihydrogenphosphate, methanesulfonate, methylsulfate, maleate, fumarate and naphthalene-2-sulfonate.
According to another of its features, the present invention relates to a process for the preparation of the compounds of formula (I), which comprises reacting an amine ##STR7## with a nitrile of the formula
X--CN (1)
in which X is a group selected from chloromethyl, 2-chloroethyl, 2-chlorovinyl, 3-chloropropyl, 4-chlorobutyl and 5-chloropentyl groups, to give the nitrile intermediate of the formula ##STR8## in which m is n-1, n being as defined above, subjecting the resulting product to catalytic hydrogenation, treating the resulting amino derivative of the formula ##STR9## with lauroyl chloride and, if desired, converting the resulting product to one of its salts.
The compounds (I) according to the invention are prepared by reacting the amine ##STR10## with a nitrile
X--CN (1)
either, if X is a vinyl group, in an alkanol such as, for example, ethanol, the reaction mixture being stirred for 2 to 4 hours at room temperature,
or, if X is other than a vinyl group, in an inert solvent such as, for example, toluene or benzene, in which the nitrile is dissolved, in the presence of a base such as triethylamine, the amino derivative then being added slowly to this solution at room temperature, to give the nitrile intermediate of formula (2) ##STR11## in which R.sub.1, R.sub.2 and m are as defined above. Acrylonitrile and chloroacetonitrile are advantageously used as compounds of formula (1).
This nitrile intermediate is then reduced at room temperature and atmospheric pressure by catalytic hydrogenation in the presence of a catalyst such as, for example, Raney nickel or lithium aluminum hydride, in an alkanol such as, for example, ethanol, in the presence of ammonia.
The resulting amino intermediate of formula (3) ##STR12## is then dissolved in an inert solvent such as, for example, methylene chloride, in the presence of a base such as, for example, triethylamine. Lauroyl chloride dissolved in methylene chloride, for example, is then added to this solution and the reaction takes place virtually instantaneously at room temperature.
The resulting product of formula (I) is isolated, in the form of the free base or a salt, by the conventional techniques.
If the compound of formula (I) is obtained in the form of the free base, salt formation is effected by treatment with the chosen acid in an organic solvent. Treatment of the free base, dissolved in an alcohol such as isopropanol, for example, with a solution of the chosen acid in the same solvent gives the corresponding salt, which is isolated by the conventional techniques. The hydrochloride, hydrobromide, sulfate, hydrogensulfate, dihydrogenphosphate, methanesulfonate, methylsulfate, oxalate, maleate, fumarate and naphthalene-2-sulfonate, for example, are prepared in this way.
When the reaction is complete, the compound of formula (I) can be isolated in the form of one of its salts, for example the hydrochloride or the oxalate; in this case, if necessary, the free base can be prepared by neutralization of said salt with a mineral or organic base such as sodium hydroxide or triethylamine, or with an alkali metal carbonate or bicarbonate such as sodium or potassium carbonate or bicarbonate.

The following examples illustrate the invention without however limiting it.
The melting points (m.p.) are expressed in degrees Celsius and were determined with a Tottoli apparatus; the yields Y are expressed in %; m denotes the weights obtained.
EXAMPLE 1
N-(3-Benzylmethylaminopropyl)lauramide hydrochloride: SR 45583 A
A) 3-(Benzylmethylamino)propionitrile
5.3 g of acrylonitrile are added dropwise to a solution of 12.1 g of N-benzylmethylamine in 20 ml of ethanol. The reaction mixture is stirred for three hours at room temperature and then concentrated under vacuum to give 17 g (Y=97%) of the expected product.
B) 3-(Benzylmethylamino)propylamine
17 g of the product obtained according to A) are dissolved in a mixture of 200 ml of ethanol, 20 ml of water and 40 ml of ammonia and hydrogenated at room temperature and atmospheric pressure in the presence of Raney nickel.
When the theoretical volume of hydrogen has been absorbed, the catalyst is filtered off and the filtrate is concentrated under vacuum. The residue is solubilized in hot ethanol and the precipitate is then filtered off and washed with ether.
m=20 g-Y=80%
C) SR 45583 A
4.2 g of lauroyl chloride are added dropwise to a solution of 5 g of the product obtained according to B) and 6 g of triethylamine in 100 ml of methylene chloride. The reaction mixture is stirred overnight at room temperature and then concentrated. The residue is taken up in water and then extracted with ethyl ether and the extract is washed with water, dried over magnesium sulfate and concentrated under vacuum.
7 g (Y=94%) of base are obtained which are dissolved in acetone, a solution of hydrogen chloride in ether is then added and the hydrochloride is filtered off and recrystallized from a mixture of isopropanol and ether. The hydrochloride is filtered off, washed with acetone and dried under vacuum.
m=4.7 g
M.p.=113.degree.-115.degree. C.
EXAMPLE 2
N-[2-(4-Benzylpiperidino)ethyl]lauramide hydrochloride: SR 44944 A
A) (4-Benzylpiperidino)acetonitrile
40.8 ml of triethylamine and 22.2 g of chloroacetonitrile are dissolved in 180 ml of toluene, and 51.6 g of 4-benzylpiperidine are then added dropwise all at once.
The temperature of the mixture rises and triethylamine hydrochloride precipitates. After a reaction time of 2 hours, the mixture is concentrated under vacuum, the residue is taken up in 600 ml of ethyl ether and the triethylamine hydrochloride is filtered off.
The filtrate is washed with a saturated solution of sodium bicarbonate, then with water and then with a saturated solution of sodium chloride, dried over magnesium sulfate, filtered and concentrated under vacuum.
The residue is distilled under reduced pressure.
m=40 g
Boiling point under 0.03 mbar=136.degree. C.
B) 1-(2-Aminoethyl)-4-benzylpiperidine dihydrochloride
22 g of the product obtained above, dissolved in a mixture of 250 ml of ethanol, 30 ml of water and 100 ml of ammonia, are hydrogenated at room temperature and atmospheric pressure in the presence of a catalytic amount of Raney nickel.
After 4 hours, the catalyst is filtered off and the filtrate is evaporated under reduced pressure. The residue is dissolved in 500 ml of methylene chloride, and 50 ml of a solution of hydrogen chloride in ether are added. The hydrochloride is filtered off and rinsed with acetone.
m=22 g
M.p.=176.degree.-180.degree. C.
C) SR 44944 A
20.38 g of the product obtained above and 29.1 ml of triethylamine are dissolved in 400 ml of methylene chloride.
A solution of 16.65 ml of lauroyl chloride in 50 ml of methylene chloride is then added.
The reaction is virtually instantaneous.
The solvent is concentrated under vacuum, the residue is taken up in ethyl ether and the triethylamine hydrochloride is filtered off.
The filtrate is washed with water, then with a saturated solution of sodium bicarbonate and then with water until the pH of the washings is neutral.
The ether phase is dried over magnesium sulfate and then concentrated under vacuum. The residue is taken up in 250 ml of acetone, and 20 ml of a solution of hydrogen chloride in ether are added. The hydrochloride is filtered off.
m=25.6 g-Y=76%
M.p.=103.degree. C.
The compounds collated in Table I were synthesized according to Examples 1 or 2.
TABLE I__________________________________________________________________________ ##STR13## Example n.sup.o SR n.sup.o ##STR14## n base or salt M.p., .degree.C. solvent Recrystallization__________________________________________________________________________45320 A 3 ##STR15## 3 150-152 hydrochloride acetone/ethyl ether45525 4 ##STR16## 2 104-106 base acetone/ethyl ether__________________________________________________________________________
The bactericidal and fungicidal activity of the products according to the invention was studied on different microbial strains by two methods.
For the purpose of comparing it with the compounds forming the subject of the invention, the product described in Czechoslovak patent 223,443: ##STR17## was synthesized and studied.
In the two methods, a bacterial inoculum is brought into contact with different dilutions of the product for a determined period of time.
When the contact period has ended, an aliquot of the mixture of bacterial suspension and product is taken and brought into contact with an agent for neutralizing the bacterial activity of the mixture, either in a solid medium or in a liquid medium. The bactericidal activity of the product, expressed either as a concentration or as a percentage of active principle, corresponds to an absence of bacterial growth.
The first method makes it possible to define a minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC), expressed in .mu.g/ml. The contact time between product and bacterial suspension is 30 minutes. The neutralizer is included in agar.
The CIP bacterial strains chosen for the study, which are available from the Institut Pasteur, are:
______________________________________1. Staphylococcus aureus CIP 531542. Enterococcus faecium CIP 58553. Escherichia coli CIP 541274. Psuedomonas aeruginosa CIP A22______________________________________
The strains are maintained on Tryptic Soy Agar.RTM. (TSA) marketed by Difco.
After 24 hours of culture at 37.degree. C., the microbial growth is harvested with the aid of glass beads and 10 ml of diluent containing 1 g of tryptone and 8.5 g of sodium chloride in 1000 ml of distilled water. The suspension formed is shaken and the percentage transmission of light at 620 nm is measured in a spectrophotometer:
Strain 1: 70%
Strain 2: 60%
Strain 3: 70%
Strain 4: 60%
The bacterial inoculum corresponds to a 1:20 dilution of this bacterial suspension.
Plates containing cupules receive different dilutions of the test product. These dilutions of the test product are brought into contact with the different bacterial suspensions using a multiple site inoculator. After a contact time of 30 minutes, aliquots are transferred with this inoculator to the surface of an agar medium (TSA) placed in Petri dishes containing an activity neutralizer, namely 20 g of lubrol W, 2.5 g of Tween 80, 2.5 g of sodium thiosulfate and 1% of egg yolk in 1000 ml of TSA (Difco). A control for the efficacy of the neutralizer is prepared for each test product by depositing an aliquot of the dilution of the test product on the surface of the culture medium. After drying, the corresponding inoculum is deposited in the same place. An inoculum control is prepared on agar medium with and without neutralizer. The results are read off after 48 hours of incubation at 37.degree. C.
The antifungal activity of the products according to the invention was also determined using the method described above. A representative strain of yeast was selected for the study: Candida albicans CIP 1180 (strain 5).
This is maintained on Sabouraud's Dextrose Agar marketed by Difco. The technique is identical to that described for studying the antibacterial activity. After 48 hours of culture at 37.degree. C., the microbial growth is harvested with the aid of glass beads and 5 ml of diluent containing 1 g of tryptone and 8.5 g of sodium chloride in 1000 ml of distilled water; a further 5 ml of the diluent are then added. This suspension gives a percentage transmission of light at 620 nm of 2 to 3% in a spectrophotometer.
The inoculum corresponds to a 1:10 dilution of this microbial suspension. A 1:100 dilution of this suspension, observed between slide and cover glass through a No. 40 microscope objective, must show 10 cells per field, which corresponds to 1,000,000 yeasts per ml.
The results are collated in Table II. They indicate the minimum bactericidal concentrations (MBC) for strains 1, 2, 3 and 4 and the minimum fungicidal concentrations (MFC) for strain 5.
TABLE II______________________________________Minimum bactericidal and fungicidal concentrations in .mu.g/ml S. aureus E. E. coli P. C.Product CIP faecium CIP aeruginosa albicanssolubility 53154 CIP 5855 54127 CIP A22 CIP 1180______________________________________D.sub.1 50 50 50 50 2001.8% inH.sub.2 OSR 45320 A 10 10 10 10 101% in TEGSR 45525 50 10 10 50 50>5% inH.sub.2 OSR 45583 A 50 10 10 50 50>5% inH.sub.2 OSR 44944 A 10 10 10 50 101% inacetone______________________________________ TEG = tetraethylene glycol
The products are all more active than the product of the prior art, D.sub.1, irrespective of the strain studied, the greatest difference being observed in respect of Candida albicans. The product having the lowest MBC and MFC is SR 45320 A.
The second method makes it possible to define a percentage of bactericidal or fungicidal active principle by means of a simplified and automated adaptation of Afnor standards NF T 72-150 and NF T 72-170. In this method, the bactericidal activity of the product is determined with and without interfering substances. It is based on assessing the destruction of 99.99% of the initial bacteria by the product in 5 minutes. The interfering substances are of the protein type and are chosen for the actual conditions of application of this type of product. The contact time between product and bacterial suspension is 5 minutes, the neutralization step is 10 minutes and subculture is effected in a liquid medium.
The chosen bacterial strains are:
______________________________________1. Staphylococcus aureus CIP 531542. Enterococcus faecium CIP 58553. Escherichia coli CIP 541274. Pseudomonas aeruginosa CIP A225. Mycobacterium smegmatis CIP 7326______________________________________
The strains are maintained on Tryptic Soy Agar.RTM. (TSA) marketed by Difco.
The strains are harvested in a manner identical to the first method; these suspensions contain between 1.10.sup.8 and 3.10.sup.8 cells per milliliter. Appropriate dilutions make it possible to have a suspension containing 2 to 6.10.sup.3 cells per milliliter.
The experiment is performed in microtiter plates; it comprises two steps. The first step, called the preliminary test, checks that the product is indeed neutralized. In the test proper, the different dilutions of the product are brought into contact with 2 to 6.10.sup.3 cells per milliliter of the bacterial suspension in the second row of the microtiter plate. After a contact time of 5 minutes, an aliquot is transferred to the third row containing the neutralizer adapted to the product and to the strain. After a contact time of 10 minutes, aliquots are transferred to the last 5 rows containing Tryptic Soy Broth.RTM. (TSB) from Difco.
The interfering substances tested are a mixture of albumin and yeast extract. In the first row of the microplate, these proteins are brought into contact for 5 minutes with the different dilutions of the product. After this step, the same principle as above is applied: 5 minutes of contact with the germ, 10 minutes of contact with the neutralizer, transfer to TSB.
The antifungal activity of the products according to the invention was also determined with and without interfering substances using the method described above. The same strain was used as for the first technique, namely Candida albicans CIP 1180. It is harvested in an identical manner to that described in the first method.
The results are collated in Table III.
TABLE III__________________________________________________________________________Results in percentage of active principle SR SR SR SRStrain Method D.sub.1 45320A 45525A 45583A 44944A__________________________________________________________________________S. aureus Simple (1) 0.1-0.05* 0.005 0.05 0.05 <0.0153154 CIP Proteins (2) 0.5 0.05 0.5 0.5 0.1E. faecium Simple (1) 0.01 <0.001 0.01 0.01 <0.015855 CIP Proteins (2) 0.1 0.1 0.5 0.05 0.03E. coli Simple (1) 0.05 0.01 0.005 0.05 0.0154127 CIP Proteins (2) 0.1 0.05 0.1 0.1 <0.01P. aeruginosa Simple (1) 0.05 0.005 0.005 0.01 <0.01A22 CIP Proteins (2) 0.5 >0.5 0.5 0.5 0.25C. albicans Simple (1) 0.1 0.005 0.05 0.05 0.011180 CIP Proteins (2) 0.5 0.05 0.5 0.1 0.06M. smegmatis Simple (1) 0.1-0.05* 0.1-0.005 0.1-0.01* 0.01 >0.57326 CIP Proteins (2) >0.5 0.05 0.1 0.05 0.06__________________________________________________________________________ *activity limited to the zone indicated (1) without interfering substances (2) with proteins as interfering substances
The product D.sub.1 of the prior art is that for which the activities are the lowest.
These results show that the products according to the invention possess an advantageous and rapid antifungal activity.
The tolerance of the products according to the invention was studied on guinea-pigs. The animals are shaved on either side of the median line of the back and shaving is repeated every 2 days. Groups of 6 animals receive 0.2 ml of an aqueous or alcoholic solution of the product according to the invention on the shaved area. If the products are in alcoholic solution, a control group of animals receives the alcohol on one side.
To study the skin tolerance, the treatment is applied once a day for 6 out of 7 days over 3 weeks. The observations pertaining to the skin concern the presence of erythema, skin eruption or hyperkeratosis, the intensity of which is graded according to a fixed scale.
The skin sensitization test is performed on the same animals after two weeks' rest. The treatment lasts one week and is identical to the previous treatment. Evaluation is made according to the same criteria and on the same scale as that used for the local tolerance.
It was found that the products according to the invention are well tolerated when they are applied at concentrations ranging up to 2% by weight. Furthermore, they have no sensitizing effect.
The acute toxicity was evaluated by oral administration to mice. This study was performed on male mice of the CD1 strain originating from the Charles River breed. Each group was made up of 5 animals with a body weight varying between 24 and 30 g, kept in the same cage. The animals were fasted for 6 hours before the treatment. For each study, a suspension of the product in a 10% solution of gum arabic was administered by force-feeding using a stomach tube. The animals were given food again 4 hours after force-feeding and were kept under observation for a period of 14 days after administration. During this period, the mortality in each of the experimental groups was noted and, where possible, the 50% lethal dose (LD.sub.50) was determined using the method of J. T. LITCHFIELD and R. WILCOXON, J. Pharmacol. 1949, 95, 99-113. The LD.sub.50 per os of the products according to the invention was found to be greater than 1000 mg/kg.
The products according to the invention, which have a good antimicrobial activity, can be used in pharmaceutical, disinfectant, cosmetic or edible preparations, especially as antiseptics by local and general administration, as disinfectants and as preservatives.
As antiseptics for human or veterinary use, the concentration of active product can vary from 0.01% to 5% by weight, according to the use and the chosen formulation. Thus it is possible to prepare detergent foaming solutions with which the surgeon and nursing staff can wash their hands or which are intended for the cleansing of dermatological lesions such as impetigo, pityriasis and leg ulcers. Detergent foaming solutions are also used as shampoos (for example antidandruff shampoos) or for the preparation of shower gels, shaving creams and foaming lotions. Foaming solutions containing products according to the invention are obtained by using amphoteric, anionic, cationic or non-ionic surfactants at a concentration of 0.3 to 30%, humectants, such as glycols or polyethylene glycols, at a concentration of 0 to 20%, copolymers of ethylene oxide and polypropylene at a concentration of 0 to 20%, and an alcohol (ethanol, isopropanol, benzyl alcohol) or a polyol, such as glycerol, at a concentration of 0 to 15%, as well as agents for complexing Ca.sup.++, Mg.sup.++ and heavy metal ions, salts providing an appropriate buffer capacity, agents for imparting viscosity, such as NaCl or KCl, natural, cellulosic or synthetic polymers, such as polyvinylpyrrolidone, superfatting and thickening agents, such as polyethylene glycol distearate and copra monoethanolamide or diethanolamide, perfumes, preservatives and colorants.
If the product according to the invention is not readily soluble in water, it will be possible to use microemulsions, micellar solutions or any other phase of the ternary or quaternary diagram water/active principle/surfactant/cosurfactant (see Les Microemulsions (Microemulsions), LA RECHERCHE No. 167, June 1985) which permits solubilization in water. These solutions may or may not be diluted; they can be dispensed for example with the aid of a vasopump or liquefied or non-liquefied propellant gases.
With the same constituents at appropriate concentrations, products according to the invention can also be used to prepare simple aqueous solutions or aqueous solutions in the form of sprays intended for antisepsis of the operative fields, for postoperative treatment and for the treatment of burns, superinfected eczema, bed-sores, wounds or acne, or intended for deodorants.
Simple alcoholic solutions or alcoholic solutions in the form of sprays containing 20 to 80% of alcohol can contain, in addition to the excipients used in the aqueous solutions, excipients which make it possible to penetrate the keratinized layers of the skin and superficial body growths, such as Azone (marketed by Nelson Research) and Transcutol (marketed by Gattefosse). These solutions are intended for antisepsis of the skin before puncturing, preparation of the operative field and antisepsis of the nursing staff's hands, or for the treatment of closed infected dermatosis, folliculitis, perionyxis or acne.
The products according to the invention can be applied in the form of creams which contain some of the compounds mentioned for the preparation of the solutions, as well as the fatty substances normally encountered in the preparation of creams or emulsions. These creams can be used especially for preventing superinfections of bed-sores, eczema, mycosis or acne.
The products according to the invention can also be used for the treatment or prevention of sexually transmitted diseases, in the form of pessaries, gynecological tablets or gynecological sponges or to complement contraceptives. The pessaries can contain from 0 to 99% of triglycerides, polyethylene glycols of different molecular weights, Tweens, natural or synthetic polymers, polyols and soaps. The gynecological tablets can contain diluents such as lactose or cellulose, lubricants such as magnesium stearate, flow enhancers such as silica, and disintegrating agents such as carboxymethyl starch or cellulose.
The products according to the invention can be administered in the form of sprays, with nasal and buccal nozzles, for the treatment of infectious syndromes of the respiratory tract (rhinitis, sinusitis, sore throat, amygdalitis, pharyngitis) or in the form of gels or mouthwashes for the treatment of gingivitis or pyorrhea or for the prevention of dental plaque, for which it will also be possible to use toothpastes containing the product according to the invention. The forms intended for buccal or nasal administration can contain the same excipients as the aqueous solutions described above, to which there may be added flavorings in the case of buccal administration or the constituents necessary for isotonicity in the case of the nasal sprays; the toothpastes also contain pyrogenic or non-pyrogenic colloidal silicas, calcium carbonate, sweeteners and fluorine salts.
The products according to the invention can be used in eye lotions, eye solutions or ophthalmic ointments for the treatment of eye infections (for example blepharitis or conjunctivitis) or in a liquid for rinsing contact lenses. These forms for the eyes can be prepared using the same constituents as those used for the solutions, care being taken to ensure that the mixture is isotonic.
Furthermore, the products according to the invention can be administered to man by a general route, for example orally, in the form of gelatin capsules, intestinal tablets or other tablets, as intestinal antiseptics.
The products according to the invention can also be used in animals for indications such as the prevention or treatment of infected lesions or lesions capable of becoming superinfected. The pharmaceutical compositions here are similar to those used in man and are, in particular, creams, sprays or solutions.
In another connection, the rapid lethal action of the products according to the invention against germs enables them to be used as surface disinfectants at concentrations which can vary from 0.1 to 4%. In this case, the products are used in preparations such as aqueous or non-aqueous, detergent foaming solutions, nasal sprays or other sprays. Such preparations are particularly useful in the hospital or veterinary sectors, for local organizations or for the agri-foodstuffs industries. These preparations can contain the same constituents as those employed in the formulations for antiseptic use, although a variety of organic solvents may be added.
Finally, the antimicrobial activity of these products enables them to be used as preservatives in the pharmaceutical, cosmetic and food industries. In this case, the products according to the invention are used as additives for pharmaceutical, cosmetic or edible formulations at concentrations which can vary from 0.005 to 0.5%. These compounds can also be used as disinfectant additives in paints.
EXAMPLE 5
Antiseptic, detergent, foaming liquid preparation
______________________________________SR 45320 A 0.5 gSodium paraffinsulfonate 15 gSodium hydroxide or lactic acid q.s. pH 5.2Purified water q.s. 100 g______________________________________
EXAMPLE 6
Antiseptic alcoholic solution
______________________________________SR 44944 A 0.2 gAlkyldimethylcarboxymethylamine (30% solution) 0.5 gCondensation product of ethylene oxide and 1 gpropylene glycol L 62Lactic acid or sodium hydroxide q.s. pH 6.570.degree. ethyl alcohol q.s. 100 g______________________________________
EXAMPLE 7
Antiseptic, detergent, foaming liquid preparation
______________________________________SR 44944 A 0.1 gAlkyldimethylcarboxymethylamine (30% solution) 15 gDisodium tetracemate 0.1 gPropylene glycol 20 gSodium hydroxide q.s. pH 5.8Purified water q.s. 100 g______________________________________
EXAMPLE 8
Oral medication
______________________________________SR 45320 A 0.3 g95.degree. ethyl alcohol 14 gOil of aniseed 0.00225 mlEugenol 0.00075 mlGlycerol 20 mlSaccharin 0.03 gSodium hydroxide solution q.s. pH 5.5Purified water q.s. 100 ml______________________________________
EXAMPLE 9
Antiseptic pessaries intended for the treatment of sexually transmitted diseases
______________________________________SR 44944 A 500 mgEutectic mixture of fatty acid esters 2.568 g______________________________________
Suppocire A.RTM., marketed by Gattefosse, can be used as the eutectic mixture of fatty acid esters.
EXAMPLE 10
Eye lotion
______________________________________SR 45320 A 0.2 gSodium chloride 1.4 gWater for injectable preparations q.s. 100 ml______________________________________
EXAMPLE 11
Intestinal tablets
______________________________________SR 44944 A 200 mgHydroxypropylmethyl cellulose 6 cP 6 mgLactose 114 mgMicrocrystalline cellulose 60 mgSodium carboxymethyl starch 12 mgMagnesium stearate 8 mgFor a finished uncoated tablet of 400 mgCoatingEudragit L 100 0.9 mgDibutyl phthalate 0.9 mgAcetone 14.1 mgIsopropyl alcohol 14.1 mgFinished coated tablet of 430 mg______________________________________
EXAMPLE 12
Sprays
______________________________________SR 44320 A 2 g95.degree. ethanol 20 gPropylene glycol 5 gSodium hydroxide q.s. pH 5.5Water q.s. 100 gPropellent q.s.______________________________________
EXAMPLE 13
Film-forming spray
______________________________________SR 44320 A 0.5 gPolyvinylpyrrolidone 2 gAcrylic resin 2 g95.degree. ethanol q.s. 100 gPropellant q.s.______________________________________
EXAMPLE 14
A product according to the invention can be used as a preservative in a cream emulsion.
______________________________________Liquid paraffin 6 gMixture of cetostearyl alcohol and ethoxylated 9 gcetostearyl alcoholAnhydrous monosodium phosphate 0.300 gDisodium tetracemate 0.010 gPetroleum jelly 15 gSR 44320 A 0.100 gPhosphoric acid q.s. pH 4.5Purified water q.s. 100 g______________________________________
EXAMPLE 15
The product according to the invention can be used as a preservative in a cream for cosmetological use.
______________________________________Collagen 0.500 gCarboxypolymethylene 934 0.400 gHydrogenated lanolin 4 gPerhydrosqualene 20 gPolyethoxylated sorbitol monopalmitate 2 gSR 44944 A 0.150 gLactic acid or sodium hydroxide q.s. pH 6.5Purified water q.s. 100 g______________________________________
EXAMPLE 16
Preservative in an antisunburn oil
______________________________________Mineral oil 65/75 68 gCastor oil 8 gSesame oil 20 gIsopropyl alcohol 2 gEusolex .RTM. 1.5 gPerfume 0.4 gSR 44320 A 0.100 g______________________________________ (Eusolex .RTM. is marketed by Merck)
EXAMPLE 17
Preservative in a shampoo
______________________________________Potassium amino acid palmitate 20 gSodium alkylsulfates 2 gCopra diethanolamide 5 gLinalyl acetate 0.200 gSR 44320 A 0.05 gSodium hydroxide q.s. pH 7Purified water q.s. 100 g______________________________________
EXAMPLE 18
Preservative for fruit juice or jam
______________________________________Micronized SR 44944 A 0.02%______________________________________
EXAMPLE 19
Disinfectant for inert surfaces
______________________________________SR 44320 A 2 gDodecyldimethylcarboxydimethylamine 20 gDisodium tetracemate 2 gLactic acid q.s. pH 3.5Purified water q.s. 100 g______________________________________
EXAMPLE 20
Antiseptic, detergent, foaming liquid preparation
______________________________________SR 45583 A 0.5 gSodium paraffinsulfonate 15 gSodium hydroxide or lactic acid q.s. pH 5.2Purified water q.s. 100 g______________________________________
EXAMPLE 21
Antiseptic alcoholic solution
______________________________________SR 45583 A 0.2 gAlkyldimethylcarboxymethylamine (30% solution) 0.5 gCondensation product of ethylene oxide and 1 gpropylene glycol L 62Lactic acid or sodium hydroxide q.s. pH 6.570.degree. ethyl alcohol q.s. 100 g______________________________________
EXAMPLE 22
Antiseptic, detergent, foaming liquid preparation
______________________________________SR 45583 A 0.1 gAlkyldimethylcarboxymethylamine (30% solution) 15 gDisodium tetracemate 0.1 gPropylene glycol 20 gSodium hydroxide q.s. pH 5.8Purified water q.s. 100 g______________________________________
EXAMPLE 23
Oral medication
______________________________________SR 45583 A 0.3 g95.degree. ethyl alcohol 14 gOil of aniseed 0.00225 mlEugenol 0.00075 mlGlycerol 20 mlSaccharin 0.03 gSodium hydroxide solution q.s. pH 5.5Purified water q.s. 100 ml______________________________________
EXAMPLE 24
Antiseptic pessaries intended for the treatment of sexually transmitted diseases
______________________________________SR 45583 A 500 mgEutectic mixture of fatty acid esters 2.568 g______________________________________
Suppocire A.RTM., marketed by Gattefosse, can be used as the eutectic mixture of fatty acid esters.
EXAMPLE 25
Eye lotion
______________________________________SR 45583 A 0.2 gSodium chloride 1.4 gWater for injectable preparations q.s. 100 ml______________________________________
EXAMPLE 26
Intestinal tablets
______________________________________SR 45583 A 200 mgHydroxypropylmethyl cellulose 6 cP 6 mgLactose 114 mgMicrocrystalline cellulose 60 mgSodium carboxymethyl starch 12 mgMagnesium stearate 8 mgFor a finished uncoated tablet of 400 mgCoatingEudragit L 100 0.9 mgDibutyl phthalate 0.9 mgAcetone 14.1 mgIsopropyl alcohol 14.1 mgFinished coated tablet of 430 mg______________________________________
EXAMPLE 27
Sprays
______________________________________SR 45583 A 2 g95.degree. ethanol 20 gPropylene glycol 5 gSodium hydroxide q.s. pH 5.5Water q.s. 100 gPropellant q.s.______________________________________
EXAMPLE 28
Film-forming spray
______________________________________SR 45583 A 0.5 gPolyvinylpyrrolidone 2 gAcrylic resin 2 g95.degree. ethanol q.s. 100 gPropellant q.s.______________________________________
EXAMPLE 29
A product according to the invention can be used as a preservative in a cream emulsion.
______________________________________Liquid paraffin 6 gMixture of cetostearyl alcohol and ethoxylated 9 gcetostearyl alcoholAnhydrous monosodium phosphate 0.300 gDisodium tetracemate 0.010 gPetroleum jelly 15 gSR 45583 A 0.100 gPhosphoric acid q.s. pH 4.5Purified water q.s. 100 g______________________________________
EXAMPLE 30
The product according to the invention can be used as a preservative in a cream for cosmetological use.
______________________________________Collagen 0.500 gCarboxypolymethylene 934 0.400 gHydrogenated lanolin 4 gPerhydrosqualene 20 gPolyethoxylated sorbitol monopalmitate 2 gSR 45583 A 0.150 gLactic acid or sodium hydroxide q.s. pH 6.5Purified water q.s. 100 g______________________________________
EXAMPLE 31
Preservative in an antisunburn oil
______________________________________Mineral oil 65/75 68 gCastor oil 8 gSesame oil 20 gIsopropyl alcohol 2 gEusolex .RTM. 1.5 gPerfume 0.4 gSR 45583 A 0.100 g(Eusolex .RTM. is marketed by Merck)______________________________________
EXAMPLE 32
Preservative in a shampoo
______________________________________Potassium amino acid palmitate 20 gSodium alkylsulfates 2 gCopra diethanolamide 5 gLinalyl acetate 0.200 gSR 45583 A 0.05 gSodium hydroxide q.s. pH 7Purified water q.s. 100 g______________________________________
EXAMPLE 33
Preservative for fruit juice or jam
______________________________________Micronized SR 45583 A 0.02%______________________________________
EXAMPLE 34
Disinfectant for inert surfaces
______________________________________SR 45583 A 2 gDodecyldimethylcarboxydimethylamine 20 gDisodium tetracemate 2 gLactic acid q.s. pH 3.5Purified water q.s. 100 g______________________________________

Number	Name	Date
2369817	Groote et al.	Feb 1945
3145137	Newallis et al.	Aug 1964
3145137	Newallis et al.	Aug 1964

Number	Date	Country
887813	Jul 1949	DEX
1207803	Feb 1960	FRX
2239	Oct 1962	FRX

N-substituted lauramides, their preparation and compositions in which they are present

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