Patent Application
20060154974
The compounds of the present invention belong to a novel class of N-thiazol-2-yl-benzamide derivatives having affinity for the adenosine 2A (A2A) receptor. The compounds are A2A-receptor ligands, such as antagonists, agonists, reverse agonists or partial agonists, and are useful in the treatment of neurological and psychiatric disorders where an A2A-receptor is implicated. Examples of diseases where an A2A-receptor is implicated are Parkinson's Disease (PD), Alzheimer's Disease, Huntington's disease, epilepsia, cerebral ischemia, haemorrhagic stroke, neonatal ischemia and hypoxia, subarachnoid haemorrhage, traumatic brain injury, brain damage following cardiac arrest, and for the treatment of depression and psychosis disorders.
Adenosine is present in all cells, including neurons and glia, of mammalian organisms where it modulates a variety of important physiological processes. The action of adenosine is mediated by specific receptors, which belong to the family of G protein-coupled receptors. Four adenosine receptors have been cloned and characterized, A1, A2A, A2B and A3 (Fredholm et al, 1994, Pharmac. Rev., 46, 143-156). The main intracellular signaling pathways involve the formation of cAMP, with A1 and A3 receptors causing inhibition of adenylate cyclase and A2A and A2B receptors activating it (Olah et al, Pharacol. Ther., 2000, 85, 55-75).
All of the adenosine receptors have been located in the CNS (Impagnatiello et al, 2000, Emerg. Ther. Targets, 4, 635-644; Rosin et al, 1998, J. Comp. Neurol., 401, 163-186). The receptor of interest here, A2A, is predominantly found in dopamine-rich areas, such as the basal ganglia components; the striatum and the globus pallidus, in various mammalians, including humans. The basal ganglia, with the striatum as a central component, are involved in integration of cortical, thalamic and limbic information to produce motor behaviours (for review see Svenningson et al, Prog. Neurobiol., 1999, 59, 355-396).
In the striatum A2A and dopamine D2 receptors are found closely co-localized on the striatopallidal GABAergic neurons, forming the so-called indirect output pathway from the striatum, which is involved in motor inhibition. A2A receptors contribute to control of motor behaviour by modulating the neurotransmission of GABA, dopamine, acetylcholine and glutamate in various ways. Currently, the interactions between A2A and D2 receptors, and especially the actions of A2A antagonists, is of great interest in the treatment for Parkinson's disease (PD), which involves a decrease in dopamine levels. The A2A receptors interact tonically and antagonistically with the D2 receptors, causing a decrease in affinity of the D2 receptors for dopamin upon stimulation. Thus, A2A antagonists may be capable of enhancing the effect of endogenous dopamine as well as clinically used dopamine agonists and increase the time-period of dopaminergic drug response. (For details and Refs therein see e.g: Richardson et al, 1997, Trends Pharmacol. Sci., 18, 338-344; Svenningson et al, Prog. Neurobiol., 1999, 59, 355-396; Fuxe et al, 2001, Parkinson's Dis. Adv., 86, 345-353).
Selective A2A receptor agonists and antagonists have been widely described in pharmacological, behavioural and neuroprotective experiments in rodents and non-human primates (for reviews see: Richardson et al, 1997, Trends Pharmacol. Sci., 18, 338-344; Ribeiro et al, 2003, Prog. Neurobiol., 68, 377-392; Ongini et al, 2001, Il Farmaco, 56, 87-90; Wardas, 2003, Polish J. Pharmacology, 54, 313-326).
The close interaction of D2 and A2A receptors can be clearly exemplified in models of catalepsy, where D2 receptor antagonists as well as A2A receptor agonists induce catalepsy, which is counteracted by A2A receptor antagonists and D2 receptor agonists, respectively (see Svenningson et al, Prog. Neurobiol., 1999, 59, 355-396 and Refs therein).
Promising anti-parkinsonian effects of A2A receptor antagonists have currently been reported by many investigators. For example, both SCH58261 (2-(2-furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine) and KW-6002 (8-[(1E)-2-(3,4-dimethoxyphenyl)ethenyl]-1,3-diethyl-3,7-dihydro-7-methyl-1H-purine-2,6-dione), enhance contralateral rotations, elicited by a subtreshold dose of levodopa, in unilateral 6-OHDA (6-hydroxydopamine) lesioned mice and rats (See Ongini et al, 2001, Drug Dev. Res., 52, 379-386 and refs therein). Furthermore, KW-6002 significantly improves motor impairment induced in non-human primates by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), without causing dyskinesias, that is commonly described for long-term treatment with the dopamine agonist L-dopa (Kanda et al, 1998, Ann. Neurol., 43 (4), 507-513; Grondin et al, 1999, Neurology, 52 (1), 1673-1677; Kanda et al, 2000, Exp. Neurol, 162, 321-327).
Thus, A2A receptor antagonists show great potential as future drugs for long-term medication of PD patients, since they do not only reverse the motor impairment but also can slow down or stop the progress of the disease by promoting cell survival. Neuroprotective effects by A2A receptor antagonists have recently been reported in in vivo and in vitro models of different neurodegenerative diseases (for review see: Wardas J., Pol J Pharmacol. 2002, 54(4), 313-26 and Stone T W. Adv Exp Med Biol. 2002, 513, 249-80). A2A antagonists have been shown to be neuroprotective in different PD models like in MPTP treated mice and 6-OHDA-lesioned rats. Here, KW-6002 prevented functional loss of dopaminergic nerve terminals in the striatum as well as prevented gliosis normally induced around degenerating neurons (Ikeda et al, 2002, J. Neurochem., 80, 262-270; Hirsch et al, 1999, Adv. Neurol., 80, 9-18; Kanda et al, 2000, Ann. Neurology, 43 (4), 507-513, Lundblad et al. J. Neurochem. 2003, 84(6), 1398-410). Similar results have been obtained in experimental models of Huntington's disease (HD). In rat HD models quinolinic acid or kainate induced lesions were reduced after using adenosine A2A receptor antagonists, with a decrease in striatal cell loss and motor changes (Reggio et al, Brain Res. 831 (1-2), 12 Jun. 1999, Pages 315-318; Popoli et al, 2002, J. Neurosci., 22, 1967-1975). In addition, it has been shown that A2A receptor antagonists decrease neuronal cell death after cerebral ischemia in neonatal and adult rats and gerbils (Gao Y, Phillis J W., Life Sci. 1994, 55(3), PL61-5; Monopoli A. et al, Neuroreport, 1998, 9(17), 3955-9). A2A knock out animals have been reported to be protected from neonatal hypoxic ischemia and transient focal ischemia (Bona E. et al, Neuropharmacology, 1997, 36(9), 1327-38; Chen J F. et al, J Neurosci, 1999, 19(21), 9192-200) and from 3NP (3-nitropropionic acid) induced, presynaptic, neurotoxic glutamate release (Blum D. et al, J. Neurosci, 2003, 23 (12), 5361-9). The protective effect of A2A antagonists against neurodegeneration by glutamate release have allready been shown in a rat model of ischemic damage to the cerebral cortex (Simpson R E, J Neurochem, 1992, 58(5), 1683-90 and O'Regan M H. et al, Brain Res, 1992, 582(1), 22-6). Protection by A2A antagonists has also been reported in primary astrocytes, in a rat model of bFGF induced astrogliosis, an amyloid beta peptide 25-35 induced neurotoxicity in cerebral granule cells (CGCs) and model of QA induced neuronal cell death in rat organotypic slice cultures (Brambilla R. et al. Glia. 2003 August;43(2):190-4; Dall'Igna O P. et al. Br J. Pharmacol. 2003 April;138(7):1207-9; Tebano M T, et al. Eur J Pharmacol. 2002 Aug. 30;450(3):253-7)
Collectively, A2A receptor antagonists can efficiently protect different neurons from various forms of insult induced neurodegeneration (Abbracchio M P, Cattabeni F, Brain adenosine . . . Ann NY Acad Sci 1999 890: 79-92; Ongini E. et al Adenosine A2A receptors and neuroprotection 1997, 825: 30-48).
Adenosine and its analogues induce “depressant-like” effects in animal models of psychiatric disorders (Minor et al., 1994, Behav Neurosci 108: 265-276; Woodson et al., 1998, Behav Neurosci 112: 399-409). Moreover, these behavioural deficits were found to be reversed by adenosine A2A receptor antagonists (Minor et al., 2001, Behav Brain Res 120: 230-212). Further studies have shown that treatment with adenosine or 2-chloroadenosine increased immobility time in the mouse forced swimming test, another animal model of depression generally considered reliable (Porsolt et al., 1977, Arch Int Pharmacodyn Ther 229: 327-336).
Several compounds with dual affinity for A2A and A1 receptor subtypes, known as the 4-amino[1,2,3]triazolo[4,3-a]quinoxalines, have been shown to be active in the rat forced swimming test (Sarges et al., 1990, J Med Chem 33: 2240-2254) indicating antidepressant activity of the substances. Most recently, A2A receptor knockout mice were found to be less sensitive to “depressant” challenges than their wildtype littermates (El Yacoubi et al., 2001, Br J Pharmacol 134: 68-77). Consistent with this data, the A2A receptor antagonists SCH58261 and KW6002 reduced the total immobility time in the mouse tail suspension test (El Yacoubi et al., 2001, Br J Pharmacol 134: 68-77). The antagonists SCH58261 and ZM241385 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]-ethyl)phenol were also found to reduce immobility when administered to mice previously screened for having high immobility time, while SCH58261 reduced immobility of mice that were selectively bred for their “helplessness” in this model (El Yacoubi et al., 2001, Br J Pharmacol 134: 68-77).
Studies using A2A knockout mice suggest that these animals show a blunted response to psychostimulants such as amphetamine and cocaine, despite the fact that their expression and binding affinities of D1 and D2 receptors are unaffected (Chen et al., 2000, Neurosci 97:195-204). Moreover, inactivation of A2A receptors has been shown to selectively attenuate amphetamine-induced behavioural sensitisation (Chen et al., 2003, Neuropsychopharmacol 28: 1086-1095). In addition, A2A knockout mice show reduced startle and PPI of the acoustic startle (Wang et al., 2003), measures often used to detect antipsychotic activity. Further support is found in studies where pharmacological blockade of A2A receptors with a selective antagonist completely abolished pre-pulse imhibition (PPI) (Nagel et al., 2003, Synapse 49: 279-286). Psychostimulants, such as MK-801 and amphetamine failed to disrupt startle and PPI in A2A KO mice (Wang et al., 2003, Behav Brain Res 143: 201-207).
Thus, the available evidence suggests that adenosine A2A receptor antagonists, by specifically modulating mesostriatal or mesocorticolimbic dopaminergic pathways, may possess antidepressant and/or antipsychotic properties
Certain compounds of formula I in its broadest form, have according to Chemical Abstracts Registry database been disclosed in various chemical catalogs without indication of any pharmaceutical activity.
Certain compounds of formula I wherein R1 is i-propyl are disclosed in WO2000026202 as being useful treating proliferative disorders associated with an altered cell dependent kinase activity.
Two compounds of formula I wherein R2 is hydroxy are disclosed in DE855120 as antituberculosis agents.
Thus, A2A receptor antagonists show great potential as future drugs for long-term medication of PD patients, since they do not only reverse the motor impairment but also can slow down or stop the progress of the disease by promoting cell survival.
Hence, there is a desire for novel A2A receptor antagonists.
The objective of the present invention is to provide compounds that are antagonists at the A2A receptor.
Accordingly, the present invention relates to the use of compounds of formula I
wherein R1 and R6 are independently hydrogen, C1-6-alkyl or halogen;
R2-R5 are independently selected from the group consisting of hydrogen, halogen, cyano, OH, NH2, nitro, C1-6-alkyl, aryl, aryl-C1-6-alkyl, heteroaryl-C1-6-alkyl, C3-8-cycloalkyl, C3-8-cycloalkyl-C1-6-alkyl, C1-6-alkoxy, aryl-C1-6-alkoxy, C1-6-alkyl-amino and aryl-C1-6-alkylamino wherein each alkyl, alkoxy or aryl may be optionally substituted with one or more halogen, cyano, C1-6-alkyl, C1-6-alkoxy, or C1-6-alkoxy-C1-6-alkoxy;
or R4 and R5 together are X—(CH2)n—Y, wherein X and Y independently are selected from the group consisting of CH2, and NH and O, n is 1, 2 or 3, and R2 and R3 are as defined above;
A is *NR8—CO, *CO—NR9, *NR8—CS or *CS—NR9 in which R8 and R9 are independently selected from the group consisting of hydrogen and C1-6-alkyl, or R8 together with R3 are C2-3-alkylene or CH2CH2O wherein the oxygen is attached to the phenyl ring, and the * indicates the atom that is attached to the phenyl ring;
and R7 is selected from the group consisting of C1-8-alkyl, aryl, heteroaryl, aryl-C1-6-alkyl, heteroaryl-C1-6-alkyl, C3-8-cycloalkyl, C3-8-cycloalkyl-C1-6-alkyl, C1-6-alkoxy, aryl-C1-6-alkoxy, heteroaryl-C1-6-alkoxy, C1-6-alkylamino, aryl-C1-6-alkylamino, heteroaryl-C1-6-alkylamino, di-(C1-6-alkyl)-amino, 2,3-dihydrobenzo-[1,4]dioxin-2-yl or adamantan-1-yl-methyl wherein each alkyl and cycloalkyl may be optionally substituted with one or more halogen, cyano, hydroxy, oxo, C1-6-alkoxy or NR10R11, wherein R10 and R11 independently are hydrogen or C1-6-alkyl, or R10 and R11 together with the nitrogen form a 5, 6 or 7 membered aliphatic ring which optionally may contain one further heteroatom selected from N and O, and each aryl may be optionally substituted with one or more halogen, cyano, hydroxy, nitro, C1-6-alkyl, C1-6-alkoxy, C1-6-acyl, C1-6-acyloxy, NR10R11 wherein R10 and R11 independently are hydrogen or C1-6-alkyl or R10 and R11 together with the nitrogen form a 5, 6 or 7 membered aliphatic ring which optionally may contain one further heteroatom selected from N and O, or a group Z-(CH2)m—W, wherein Z and W are attached to two adjacent carbon atoms and independently are selected from the group consisting of CH2, NH and O, and m is 1, 2 or 3, provided that when R7 is attached to nitrogen, then R7 is not C1-6-alkoxy, aryl-C1-6-alkoxy, heteroaryl-C1-6-alkoxy, C1-6-alkylamino, aryl-C1-6-alkylamino, heteroaryl-C1-6-alkylamino or di-(C1-6-alkyl)-amino;
and pharmaceutically acceptable addition salts thereof;
for the manufacture of a medicament for treatment of a disease where an A2A-receptor is implicated.
In a second aspect the present invention relates to a pharmaceutical composition comprising compounds of formula I as defined above provided that if A is *NR8CO, and R1-6 and R8 all are hydrogen, then R7 is not thiophen-2-yl;
and provided that if A is *NR8—CO, R2-6 and R8 all are hydrogen, and R1 is i-propyl then R7 is not methyl or benzyl;
and provided that if A is *NR8—CO, R2, R4-6 and R8 all are hydrogen, R3 is iodine and R1 is i-propyl then R7 is not methyl;
and provided that if A is *NR8—CO, R1, R3-6 and R8 all are hydrogen and R2 is hydroxy then R7 is not methyl or ethoxy.
In a third aspect the present invention relates to compounds of formula I as defined above provided that if A is *NR8—CO, and R1-6 and R8 all are hydrogen, then R7 is not selected from the group consisting of C1-4-alkyl, pentan-3-yl, trifluoromethyl, pyrimidyl, furan-2yl, thiophen-2-yl, substituted or unsubstituted phenyl or substituted or unsubstituted benzyl;
and provided that if A is *NR8—CO, R2-6 and R8 all are hydrogen, and R1 is i-propyl then R7 is not methyl or benzyl;
and provided that if A is *NR8CO, R2, R4-6 and R8 all are hydrogen, R3 is iodine and R1 is i-propyl then R7 is not methyl;
and provided that if A is *NR8—CO, R1, R3-6 and R8 all are hydrogen and R2 is hydroxy then R7 is not methyl or ethoxy;
and provided that if A is *NR8—CO, R2, R4-6 and R8 all are hydrogen, R2 is nitro and R7 is methyl then R1 is not hydrogen or methyl.
and provided that if A is *CO—NR9, R1, R6 and R9 all are hydrogen, and R7 is thiazol-2-yl, then R2-5 are not all hydrogen or all fluor;
and provided that if A is *CO—NR9, R2-5 and R9 all are hydrogen, and R6 is methyl, then R1 may not be hydrogen if R7 is 4-methyl-thiazol-2-yl and R1 may not be methyl if R7 is 4,5-dimethyl-thiazol-2-yl.
The compounds of the invention are A2A receptors antagonists having a human A2A binding affinity (Ki) of 5 μM or less, typically of 1 μM or less, preferably of 550 nM or less, more preferred of 200 nM or less, even more preferred of 50 nM or less and most preferred of 10 nM or less.
In a particular embodiment the present invention relates to use of such compounds for the manufacture of a medicament for the treatment of a disease where an A2A-receptor is implicated, is selected from the group consisting of Parkinson's Disease (PD), Alzheimer's Disease, Huntington's disease, epilepsia, cerebral ischemia, haemorrhagic stroke, neonatal ischemia and hypoxia, subarachnoid haemorrhage, traumatic brain injury, brain damage following cardiac arrest, and for the treatment of depression and psychosis disorders.
In a more particular embodiment the present invention relates to use of such compounds for the manufacture of a medicament for the treatment of Parkinson's Disease.
In a particular embodiment the present invention relates to such compounds wherein A is *NR8—CO or *CO—NR9, more particularly *NR8CO.
In another particular embodiment the present invention relates to such compounds wherein R7 is selected from the group consisting of C1-8-alkyl, preferably C3-8-alkyl and even more preferred C4-8-alkyl which is branched at the β-position, C3-8-cyclo-alkyl-methyl, C3-8-cyclalkyl, methylphenyl, methoxybenzyl and thiophen-2-yl-methyl, wherein each alkyl or cycloalkyl may be unsubstituted or substituted with oxo.
In another particular embodiment the present invention relates to such compounds wherein R8 is hydrogen.
In another particular embodiment the present invention relates to such compounds wherein R9 is hydrogen.
In another particular embodiment the present invention relates to such compounds wherein R6 is hydrogen.
In another particular embodiment the present invention relates to such compounds wherein R1 is hydrogen, methyl or chloro, preferably hydrogen.
In yet another particular embodiment the present invention relates to such compounds wherein R2-5 are independently selected from the group consisting of hydrogen, halogen, C1-6-alkyl, preferably methyl, C1-6-alkoxy and C1-6-alkoxy-C1-6-alkoxy, preferably 2-methoxy-ethoxy.
In a more particular embodiment the present invention relates to such compounds wherein R2 and R4 are independently selected from the group consisting of hydrogen, C1-6-alkoxy and C1-6-alkoxy-C1-6-alkoxy, preferably 2-methoxy-ethoxy.
In another more particular embodiment the present invention relates to such compounds wherein R3 and R5 are independently selected from the group consisting of hydrogen, halogen, C1-6-alkyl, preferably methyl, C1-6-alkoxy, preferably methoxy, and C1-6-alkoxy-C1-6-alkoxy, preferably 2-methoxy-ethoxy, trifluoromethyl and trifluoromethoxy.
Particular compounds of the invention are compounds 1-133 as disclosed in the examples.
The compounds of the general formula I may exist as optical isomers thereof and such optical isomers are also embraced by the invention. Throughout the specification and claims, reference to specific compounds refers to the racemates unless otherwise indicated.
The term C1-6-alkyl refers to a branched or unbranched alkyl group having from one to six carbon atoms inclusive, such as methyl, ethyl, 1-propyl, 2-propyl, 1-butyl, 2-butyl, 2-methyl-2-propyl, and 2-methyl-1-propyl. The term C1-8-alkyl refers similarly to branched or unbranched alkyl group having from one to eight carbon atoms inclusive.
The term C3-8-cycloalkyl designates a monocyclic or bicyclic carbocycle having three to eight C-atoms, such as cyclopropyl, cyclopentyl, cyclohexyl, etc.
Halogen means fluoro, chloro, bromo or iodo.
As used herein, the term acyl refers to a formyl, C1-6-alkylcarbonyl, arylcarbonyl, aryl-C1-6-alkylcarbonyl, C3-8-cycloalkylcarbonyl or a C3-8-cycloalkyl-C1-6-alkylcarbonyl group.
The terms C1-6-alkoxy, C3-8-cycloalkyl-C1-6-alkyl, aryl-C1-6-alkyl, heteroaryl-C1-6-alkyl, C1-6-alkylamino, C1-6-alkylcarbonyl, and the like, designate such groups in which the C1-6-alkyl, aryl, heteroaryl and the C3-8-cycloalkyl group are as defined above.
The term aryl refers to a carbocyclic aromatic group, such as phenyl or naphthyl, in particular phenyl.
The term heteroaryl refers to 5-membered monocyclic rings such as 1H-tetrazolyl, 3H-1,2,3-oxathiazolyl, 3H-1,2,4-oxathiazolyl, 3H-1,2,5-oxathiazolyl, 1,3,2-oxathiazolyl, 1,3,4-oxathiazolyl, 1,4,2-oxathiazolyl, 3H-1,2,4-dioxazolyl, 1,3,2-dioxazolyl, 1,4,2-dioxazolyl, 3H-1,2,3-dithiazolyl, 3H-1,2,4-dithiazolyl, 1,3,2-dithiazolyl, 1,4,2-dithiazolyl, 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1H-1,2,3-triazolyl, 1H-1,2,4-triazolyl, isoxazolyl, oxazolyl, isothiazolyl, thiazolyl, 1H-imidazolyl, 1H-pyrazolyl, 1H-pyrrolyl, furanyl, thienyl, 1H-pentazole; 6-membered monocyclic rings such as 1,2,3-oxathiazinyl, 1,2,4-oxathiazinyl, 1,2,5-oxathiazinyl, 4H-1,3,5-oxathiazinyl, 1,4,2-oxathiazinyl, 1,4,3-oxathiazinyl, 1,2,3-dioxazinyl, 1,2,4-dioxazinyl, 4H-1,3,2-dioxazinyl, 4H-1,3,5-dioxazinyl, 1,4,2-dioxazinyl, 2H-1,5,2-dioxazinyl, 1,2,3-dithiazinyl, 1,2,4-dithiazinyl, 4H-1,3,2-dithiazinyl, 4H-1,3,5-dithiazinyl, 1,4,2-dithiazinyl, 2H-1,5,2-dithiazinyl, 2H-1,2,3-oxadiazinyl, 2H-1,2,4-oxadiazinyl, 2H-1,2,5-oxadiazinyl, 2H-1,2,6-oxadiazinyl, 2H-1,3,4-oxadiazinyl, 2H-1,3,5-oxadiazinyl, 2H-1,2,3-thiadiazinyl, 2H-1,2,4-thiadiazinyl, 2H-1,2,5-thiadiazinyl, 2H-1,2,6-thiadiazinyl, 2H-1,3,4-thiadiazinyl, 2H-1,3,5-thiadiazinyl, 1,2,3-triazinyl, 1,2,4-triazinyl, 1,3,5-triazinyl, 2H-1,2-oxazinyl, 2H-1,3-oxazinyl, 2H-1,4-oxazinyl, 2H-1,2-thiazinyl, 2H-1,3-thiazinyl, 2H-1,4-thiazinyl, pyrazinyl, pyridazinyl, pyrimidyl, pyridyl, 2H-pyranyl, 2H-thiinyl; and to bicyclic rings such as 3H-1,2,3-benzoxathiazolyl, 1,3,2-benzodioxazolyl, 3H-1,2,3-benzodithiazolyl, 1,3,2-benzodithiazolyl, benzfurazanyl, 1,2,3-benzoxadiazolyl, 1,2,3-benzothiadiazolyl, 2,1,3-benzothiadiazolyl, 1H-benzotriazolyl, 1,2-benzisoxazolyl, 2,1-benzisoxazolyl, benzoxazolyl, 1,2-benzisothiazolyl, 2,1-benzisothiazolyl, benzothiazolyl, 1H-benzimidazolyl, 1H-indazolyl, 3H-1,2-benzoxathiolyl, 1,3-benzoxathiolyl, 3H-2,1-benzoxathiolyl, 3H-1,2-benzodioxolyl, 1,3-benzodioxolyl 3H-1,2-benzodithiolyl, 1,3-benzodithiolyl, 1H-indolyl, 2H-isoindolyl, benzofuranyl, isobenzofuranyl, 1-benzothienyl, 2-benzothienyl, 1H-2,1-benzoxazinyl, 1H-2,3-benzoxazinyl, 2H-1,2-benzoxazinyl, 2H-1,3-benzoxazinyl, 2H-1,4-benzoxazinyl, 2H-3,1-benzoxazinyl, 1H-2,1-benzothiazinyl, 1H-2,3-benzothiazinyl, 2H-1,2-benzothiazinyl, 2H-1,3-benzothiazinyl, 2H-1,4-benzothiazinyl, 2H-3,1-benzothiazinyl, cinnolinyl, phtalazinyl, quinazolinyl, quinoxalinyl, isoquinolyl, quinolyl, 1H-2-benzopyranyl, 2H-1-benzopyranyl, 1H-2-benzothiopyranyl or 2H-1-benzothiopyranyl.
The term rac means racemic.
The acid addition salts of the compounds of the invention are pharmaceutically acceptable salts formed with non-toxic acids. Exemplary of such organic salts are those with maleic, fumaric, benzoic, ascorbic, succinic, oxalic, bis-methylenesalicylic, methanesulfonic, ethanedisulfonic, acetic, propionic, tartaric, salicylic, citric, gluconic, lactic, malic, mandelic, cinnamic, citraconic, aspartic, stearic, palmitic, itaconic, glycolic, p-aminobenzoic, glutamic, benzenesulfonic and theophylline acetic acids, as well as the 8-halotheophyllines, for example 8-bromotheophylline. Exemplary of such inorganic salts are those with hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric and nitric acids.
The pharmaceutical compositions of this invention, or those which are manufactured in accordance with this invention, may be administered by any suitable route, for example orally in the form of tablets, capsules, powders, syrups, etc., or parenterally in the form of solutions for injection. For preparing such compositions, methods well known in the art may be used, and any pharmaceutically acceptable carriers, diluents, excipients or other additives normally used in the art may be used.
Conveniently, the compounds of the invention are administered in unit dosage form containing said compounds in an amount of about 0.01 to 100 mg.
The total daily dose is usually in the range of about 0.05-500 mg, and most preferably about 0.1 to 50 mg of the active compound of the invention.
The compounds of the invention are prepared by the following general methods:
a) Coupling of a compound with formula II
wherein R1-R8 is as described above, with a carboxylic acid R7—COOH or carboxylic acid chloride R7—COCl, wherein R7 is as defined above.
The coupling of compounds of formula II with carboxylic acids R7—COOH is performed by standard procedures knowledgeable to chemists skilled in the art. This includes coupling in the presence of a uronium salt coupling reagent and diisopropyethylamine (DIPEA), at temperatures between 20-80° C., in a suitable polar or apolar solvent such as NMP or 1,2-dichloroethane, or coupling of starting materials of formula II with carboxylic acid chlorides R7—COCl in the presence of a suitable base such as pyridine at temperatures between 20-60° C. in a suitable solvent such as 1,2-dichloroethane
b) Condensation of a compound with formula II with isocyanates R7—NCO wherein R7 is as defined above
The coupling of compounds of formula II with isocyanates R7—NCO is performed by standard procedures knowledgeable to chemists skilled in the art. This includes condensation at temperatures between 20-150° C. in a suitable polar or apolar solvent such as NMP or 1,2-dichloroethane.
c) Condensation of a compound with formula II with a chloroformate R7—OCOCl wherein R7 is as defined above.
The coupling of compounds of formula II with chloroformates R7—OCOCl is performed by standard procedures knowledgeable to chemists skilled in the art. This includes condensation at temperatures between 20-80° C. in a suitable polar or apolar solvent such as NMP or 1,2-dichloroethane in the presence of a suitable base, such as triethylamine.
d) Coupling of a compound with formula III
wherein R1-R6 is as described above, with an amine HN(R9)R7, wherein R9 and R7 is as defined above.
The coupling of compounds of formula III with amines HN(R9)R7 is performed by standard procedures knowledgeable to chemists skilled in the art. This includes coupling in the presence of a uronium salt coupling reagent and diisopropyethylamine (DIPEA), at temperatures between 20-80° C., in a suitable polar or apolar solvent such as NMP or 1,2-dichloroethane.
Compounds of formula II were prepared according to standard procedures known to chemists skilled in the art as outlined below. Suitably substituted 4-nitro benzoic acid chlorides were either commercially available or prepared by chlorination of the corresponding carboxylic acids with oxalylchloride or sulfonyl chloride, and were coupled with suitably substituted 2-amino thiazoles in a suitable solvent such as 1,2-dichloroethane in the presence of a suitable base such as pyridine, at a suitable temperature between 20-60° C. The products were then reduced to the corresponding anilines by procedures known to chemists skilled in the art, such as catalytic hydrogenation using hydrogen and a suitable catalyst such as 5% Pd/C in a suitable solvent such as ethanol; or reduction using a suitable metal reagent such as SnCl2 or Zn(s) and a suitable acid such as HCl, at a suitable temperature such as room temperature and in a suitable solvent such as acetic acid or ethanol. Alternatively, starting materials of formula II were prepared by reaction of suitably substituted N-protected 4-amino benzoic acids by chlorination of the carboxylic acid, and coupling with suitably substituted 2-amino thiazoles, under the same conditions as described above, followed by deprotection of the amino functionality under suitable conditions, such as acidolysis. Alternatively, suitably substituted 4-amino benzoic acids were coupled with suitably substituted 2-amino thiazoles in the presence of a carbodiimide coupling reagent such as 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride in the presence of a suitable additive such as 1-hydroxybenzotriazole in a suitable solvent such as 1,2-dichloroethane in the presence of a suitable base such as DIPEA, at a suitable temperature between 20-60° C.
Compounds of formula III were prepared according to standard procedures known to chemists skilled in the art as outlined below. Suitably substituted methyl 4-chlorocarbonylbenzoates were either commercially available or prepared by chlorination of the corresponding carboxylic acids with oxalylchloride or sulfonyl chloride, and were coupled with 2-amino thiazole in a suitable solvent such as 1,2-dichloroethane in the presence of a suitable base such as pyridine, at a suitable temperature between 20-60° C. The products were then saponified to the corresponding carboxylates by procedures known to chemists skilled in the art, such as treatment with 2M NaOH (aq.) at a suitable temperature such as room temperature in the presence of a suitable organic co-solvent such as THF, followed by acidification to yield the carboxylic acid products.
Experimental Section
Analytical LC-MS data were obtained by either of two methods: (method A): on a PE Sciex API 150EX instrument equipped with an IonSpray source and a Shimadzu LC-8A/SLC-10A LC system. Column: 30×4.6 mm Waters Symmetry C18 column with 3.5 μm particle size; solvent system: A=water/trifluoroacetic acid (100:0.05) and B=water/acetonitrile/trifluoroacetic acid (5:95:0.03); method: Linear gradient elution with 90% A to 100% B in 4 min and with a flow rate of 2 ml/min. or (method B): on a Micromass LCT instrument equipped with a 4-way MUX ElectroSpray source, a Micromass Waters MUX-2488 UV-detector, a Sedex 754 4-channels LT-ELS-detector, a CTC Analytics HTS-PAL autosampler equipped with 4 injection valves, and 4 Waters 1525 Binary HPLC pumps. Column: 30×4.6 mm Waters Symmetry C18 column with 3.5 μm particle size; solvent system: A=water/trifluoroacetic acid (100:0.05) and B=water/acetonitrile/trifluoroacetic acid (5:95:0.03); method: Linear gradient elution with 90% A to 100% B in 4 min and with a flow rate of 2 ml/min. Purity was determined by integration of the UV (254 nm) and ELSD traces. The retention times (RT) are expressed in minutes.
1H NMR spectra were recorded at 500.13 MHz on a Bruker Avance DRX500 instrument or at 250.13 MHz on a Bruker AC 250 instrument. Deuterated dimethyl sulfoxide (99.8% D) was used as solvent. TMS was used as internal reference standard. Chemical shift values are expressed in ppm. The following abbreviations are used for multiplicity of NMR signals: s=singlet, d=doublet, t=triplet, q=quartet, qui=quintet, h=heptet, dd=double doublet, dt=double triplet, dq=double quartet, tt=triplet of triplets, m=multiplet, br s=broad singlet and br=broad signal.
For column chromatography silica gel of the type Kieselgel 60, 40-60 mesh ASTM was used. Microwave heated experiments were performed with a Personal Chemistry Emrys Synthesiser or a Personal Chemistry Emrys Optimiser.
Preparation of Intermediates
2-Amino thiazole (100 mmol) was suspended in 1,2-dichloroethane (200 mL) and pyridine (100 mmol) was added. The mixture was added portion wise to a suspension of 4-nitro benzoic acid chloride (150 mmol) in 1,2-dichloroethane (500 mL) and stirred at 60° C. over night. The reaction mixture was cooled and filtered. The filtrate was washed with 1,2-dichloroethane and dried in vacuo.
Yield: 96%
1H NMR (D6-DMSO): 7.33 (d, 1H); 7.60 (d, 1H); 8.26-8.41 (4H); 12.96 (br s, 1H).
4-Nitro-N-thiazol-2-yl-benzamide (28 mmol) was suspended in abs. EtOH (400 mL) and ethyl acetate (200 mL) and glacial acetic acid (50 mL) was added followed by 10% Pd/C (0.5 g). The mixture was hydrogenated for 72 h at 3 bar H2. The hydrogenation mixture was filtered, and the solvent was removed under reduced pressure. The crude product was added NaHCO3 (sat.) and ethyl acetate, the remaining solid fraction was removed by filtration and dried in vacuo. The liquid phases were separated, the organics were washed with brine, dried over MgSO4, filtered and evaporated to yield a solid. The solid fractions were pure product and were combined.
Yield: 83% (80% overall).
1H NMR (D6-DMSO): 5.93 (s, 2H); 6.50 (d, 2H); 7.18 (d, 1H); 7.49 (d, 1H); 7.84 (d, 2H); 12.05 (br s, 1H).
4-Nitro-3-methyl-benzoic acid (83 mmol) was suspended in 1,2-dichloroethane (500 mL) and dimethylformamide (DMF) (5 mL) under an argon atmosphere. Oxalylchloride (2M in dichloromethane, 62.3 mL) was added slowly to the stirred suspension. After stirring at room temperature for 1 h, the solvent was removed by evaporation under reduced pressure, and the reaction mixture was re-dissolved in 1,2-dichloroethane (400 mL). A suspension of 2-amino thiazole (83 mmol) and pyridine (83 mmol) in 1,2-dichloroethane (100 mL) was added portion wise. The reaction mixture was stirred at 50° C. over night. The solvent was removed under reduced pressure and the solids were re-suspended in ethyl acetate (500 mL) and NaHCO3 (sat.) (500 mL). The solids were removed by filtration (pure product) and the liquid phases were separated. The organic phase was washed with NaHCO3 (sat.), dried over MgSO4, filtered and evaporated. The crude was re-crystallized from ethyl acetate and the product fractions were combined.
Yield: 76%.
1H NMR (D6-DMSO): 2.58 (s, 3H); 7.33 (d, 1H); 7.60 (d, 1H); 8.10 (d, 2H); 8.20 (d, 2H); 12.92 (br s, 1H).
4-Nitro-3-methyl-N-thiazol-2-yl-benzamide (63 mmol) was suspended in abs. EtOH (200 mL) and ethyl acetate (100 mL) and glacial acetic acid (10 mL) was added followed by 10% Pd/C (1 g). The mixture was hydrogenated over night at 3 bar H2. The hydrogenation mixture was filtered and the solvent was removed under reduced pressure. The crude product was added NaHCO3 (sat.) and ethyl acetate, the remaining solid fraction was removed by filtration and dried in vacuo. The liquid phases were separated, the organics were washed with brine, dried over MgSO4, filtered and evaporated to yield a solid. The solid fractions were product and were combined.
Yield: 95% (72% overall).
1H NMR (D6-DMSO): 2.09 (s, 3H); 5.71 (s, 2H); 6.63 (d, 1H); 7.17 (d, 1H); 7.39 (d, 1H); 7.69-7.81 (m, 2H); 11.96 (br s, 1H).
The following compounds were prepared analogously:
Yield: 53%
1H NMR (D6-DMSO): 3.45 (s, 3H); 6.15 (s, 2H); 6.25-6.35 (2H); 7.20 (d, 1H); 7.46 (d, 1H); 7.71 (d, 1H); 10.97 (s, 1H).
Yield: 17%
1H NMR (D6-DMSO): 3.85 (s, 3H); 5.59 (s, 2H); 6.67 (d, 1H); 7.19 (d, 1H); 7.48-7.65 (3H); 12.17 (br s, 1H).
Yield: 34%
1H NMR (D6-DMSO): 6.02 (s, 2H); 6.59 (d, 2H); 7.53 (s, 1H); 7.83 (d, 2H); 12.29 (br s, 1H).
Yield: 16%
1H NMR (D6-DMSO): 2.35 (d, 3H); 5.90 (s, 2H); 6.57 (d, 2H); 7.15 (d, 1H); 7.81 (d, 2H); 11.83 (s, 1H).
4-Nitro-2-chloro-N-thiazol-2-yl-benzamide (5 mmol) was dissolved in glacial acetic acid (20 mL) and added to a mixture of SnCl2 in HCl (conc.). The reaction was stirred at room temperature over night, then poured onto ice and neutralized with NaOH. The aqueous phase was extracted with ethyl acetate, the organics were combined, dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using a gradient of ethyl acetate in heptane.
Yield: 46% (overall)
1H NMR (D6-DMSO): 5.95 (s, 2H); 6.52 (m, 1H); 6.65 (d, 1H); 7.23 (d, 1H); 7.36 (d, 1H); 7.50 (d, 1H); 12.18 (s, 1H).
4-Acetylamino-2-methyl-benzoic acid (16 mmol) was suspended in 1,2-dichloroethane (100 mL) and DMF (1 mL) under an argon atmosphere. Oxalylchloride (2M in dichloromethane, 12 mL) was added slowly to the stirred suspension. After stirring at room temperature for 1 h the solvent was removed by evaporation under reduced pressure, and the reaction mixture was re-dissolved in 1,2-dichloroethane (80 mL). A suspension of 2-amino thiazole (16 mmol) and pyridine (16 mmol) in 1,2-dichloroethane (20 mL) was added portion wise. The reaction mixture was stirred at 50° C. over night. The solvent was removed under reduced pressure and the solids were re-suspended in ethyl acetate (100 mL) and NaHCO3 (sat.) (100 mL). The liquid phases were separated and the aqueous phase was extracted with ethyl acetate. The combined organic phases were washed with water, dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using gradient elution (heptane/ethyl acetate).
Yield: 25%.
4-Acetylamino-2-methyl-N-thiazol-2-yl-benzamide (4 mmol) was refluxed over night in HCl (8M) (50 mL). The pH was adjusted to 8 with NaOH (aq., conc.) and the product was removed by filtration, washed with water and dried in vacuo.
Yield: 13% (3% overall)
1H NMR (D6-DMSO): 2.35 (s, 3H); 5.66 (br s, 2H); 6.36-6.46 (2H); 7.17 (d, 1H); 7.42 (d, 1H); 7.48 (d, 1H).
4-Nitro-3-fluoro-benzoic acid (535 mmol) was dissolved in toluene (500 mL) and THF (75 mL). SOCl2 (930 mmol) was added and the mixture was heated at 65° C. for 5 h. The reaction mixture was cooled and the solvent removed by evaporation. The residue was re-dissolved in 1,2-dichloroethane. This solution was added dropwise to a suspension of 2-amino-thiazole (480 mmol) and DIPEA (370 mmol) in 1,2-dichloroethane (1 L) with mechanical stirring, while the temperature was kept at 45° C. Upon complete addition the reaction mixture was heated at 60° C. for 1.5 h, then allowed to cool to room temperature and stirred over night. The reaction mixture was filtered, the solids were washed with 1,2-dichloroethane and dried in vacuo.
Yield: 35%
1H NMR (D6-DMSO): 7.34 (d, 1H); 7.61 (d, 1H); 8.10 (m, 1H); 8.23 (m, 1H); 8.31 (m, 1H); 13.00 (br, 1H).
4-Nitro-3-fluoro-N-thiazol-2-yl-benzamide (7.5 mmol) was suspended in EtOH (abs., 60 mL) and ethyl acetate (30 mL), glacial acetic acid (5 mL) and 10% Pd/C (300 mg) was added, and the mixture was hydrogenated for 12 days under 3 bar H2. The reaction mixture was filtered and evaporated, and re-dissolved in ethyl acetate (100 mL) and NaHCO3 (sat., 60 mL). The aqueous phase was adjusted to basic pH with NaOH (1M) and the phases were separated. The organic phase was washed with brine, dried over MgSO4, filtered and evaporated.
Yield: 85% (30% overall)
1H NMR (D6-DMSO): 6.00 (s, 2H); 6.80 (t, 1H); 7.21 (d, 1H); 7.51 (d, 1H); 7.74 (m, 1H); 7.81 (m, 1H); 12.19 (s, 1H).
The following compounds were prepared analogously:
Yield: 16%
1H NMR (D6-DMSO): 6.19 (s, 2H); 6.35 (m, 1H); 6.43 (m, 1H); 7.21 (d, 1H); 7.48-7.55 (2H); 11.64 (br, 1H).
4-tert-Butoxycarbonylamino-benzoic acid (6.3 mmol) and 1-hydroxybenzotriazole (6.3 mmol) were combined in a flask and suspended in 1,2-dichloroethane (30 mL). 1-(3-Dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (6.3 mmol) and DIPEA (15.4 mmol) was added followed by 4,5-dimenthyl-2-amino-thiazole hydrochloride (9.1 mmol). The resulting solution was stirred at ambient temperature over night, then the reaction mixture was washed extensively with AcOH (aq., pH ˜3), dried over MgSO4, filtered and evaporated. During evaporation of the solvent the desired product precipitated and was collected by filtration, washed with 1,2-dichloroethane and dried.
Yield: 20%
1H NMR (D6-DMSO): 1.50 (s, 9H); 2.20 (s, 3H); 2.26 (s, 3H); 7.58 (d, 2H); 8.02 (d, 2H); 9.73 (s, 1H); 12.20 (br s, 1H).
[4-(Thiazol-2-ylcarbamoyl)-phenyl]-carbamic acid tert-butyl ester was deprotected prior to use by dissolution in dichoromethane/trifluoroacetic acid (1:1) for 10 min., followed by evaporation of the solvent. The residue was taken up in dichloromethane, and extracted with NaOH (0,1M). The formed precipitate was the product, and was removed by filtration, washed with water and dried.
4-Amino-benzoic acid ethyl ester (60.5 mmol) was dissolved in 1,2-dichloroethane (100 mL), and a catalytic amount of 4-(N,N-dimethylamino) pyridine was added followed by acetic acid anhydride (66.6 mmol) in 1,2-dichloroethane (15 mL). The reaction mixture was stirred at room temperature for 24 h, then the solvent was evaporated and the residue was re-dissolved in ethyl acetate (200 mL) and extracted with HCl (0.1M)×2, Na2CO3 (aq., sat.)×2, H2O and brine. The organic phase was dried over MgSO4, filtered and evaporated.
Yield: 90%
1H NMR (D6-DMSO): 1.31 (t, 3H); 2.09 (s, 3H); 4.28 (q, 2H); 7.72 (d, 2H); 7.90 (d, 2H); 10.28 (s, 1H).
4-Acetylamino-benzoic acid ethyl ester (54.5 mmol) was dissolved in THF (100 mL), and potassium tert-butoxide (54.5 mmol) was added followed by methyl iodide (60 mmol). The reaction mixture was stirred at room temperature for 1 h, then the solvent was evaporated. The crude product was used in the next reaction without further purification.
Crude 4-(Acetyl-methyl-amino)-benzoic acid ethyl ester was refluxed over night in conc. HCl (100 mL). The mixture was cooled and a small amount of sodium sulfite was added. The pH was adjusted to 4 with NaOH (aq., conc.), this gave a heavy precipitation and the solids were removed by filtration, washed with water and dried in vacuo.
Yield: 74%
1H NMR (D6-DMSO): 2.72 (d, 3H); 6.45 (q, 1H); 6.53 (d, 2H); 7.68 (d, 2H).
4-Methylamino-benzoic acid (13.2 mmol) was dissolved in DMF (4 mL) and 1,2-dichloroethane (25 mL). DIPEA (13.2 mmol), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (13.2 mmol), 1-hydroxybenzotriazole (13.2 mmol) and 2-aminothiazole (13.2 mmol) was added, and the reaction mixture was stirred at 40° C. for 72 h. HCl (2M) (13.2 mmol) was added followed by water (20 mL), upon which a solid precipitated. This was removed by filtration, washed with water and dried in vacuo.
Yield: 32% (22% overall)
1H NMR (D6-DMSO): 2.75 (s, 3H); 3.93 (br, 1H); 6.59 (d, 2H); 7.19 (d, 1H); 7.51 (d, 1H); 7.92 (d, 2H); 12.12 (br s, 1H).
4-Amino-N-thiazol-2-yl-benzamide (2.28 mmol) was suspended in THF (10 mL) and propanal (3.42 mmol) was added followed by glacial acetic acid (4.2 mmol) and NaBH(OAc)3 (4.56 mmol). The mixture was stirred at room temperature over night. More NaBH(OAc)3 (2.28 mmol) was added and stirring continued for 3.5 h. The solvent was removed by evaporation under reduced pressure and the residue was re-dissolved in ethyl acetate (100 mL) and NaHCO3 (sat.) (40 mL) (pH of the aqueous phase was adjusted to 11). The phases were separated and the aqueous phase was extracted with ethyl acetate. The combined organic extracts were washed with brine, dried over MgSO4, filtered and evaporated. The crude product was re-crystallized from EtOH.
Yield: 36%
1H NMR (D6-DMSO): 0.94 (t, 3H); 1.57 (m, 2H); 3.05 (m, 2H); 6.49 (t, 1H); 6.60 (d, 2H); 7.18 (d, 1H); 7.50 (d, 1H); 7.89 (d, 2H); 12.07 (s, 1H).
NaH (60% in oil suspension) (30 mmol) was weighed into a flask, and DMF (30 mL) was added followed by drop wise addition of 1-propanol. 2-Fluoro-4-nitro-N-thiazol-2-yl-benzamide (7.5 mmol) was added portion wise. The mixture was stirred over night at 80° C. and then poured into water (90 mL). HCl (22 mmol) was added and the aqueous phase was extracted with ethyl acetate. The organic fractions were washed with brine, dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using gradient elution (heptane/ethyl acetate).
Yield: 11%
1H NMR (D6-DMSO): 0.98 (t, 3H); 1.78 (m, 2H); 4.21 (t, 2H); 7.32 (d, 1H); 7.55 (d, 1H); 7.86-7.93 (3H).
4-Nitro-2-propoxy-N-thiazol-2-yl-benzamide (0.8 mmol) was suspended in EtOH (30 mL) and ethyl acetate (6 mL), glacial acetic acid (2.5 mL) and 10% Pd/C (40 mg) was added, and the mixture was hydrogenated under 3 bar H2 for 3 days. The reaction mixture was filtered and evaporated, re-dissolved in ethyl acetate, and extracted with NaHCO3 (sat.). The organics were dried over MgSO4, filtered and evaporated to yield the product.
Yield: 96% (10% overall)
1H NMR (D6-DMSO): 0.98 (t, 3H); 1.77 (m, 2H); 4.21 (t, 2H); 7.32 (d, 1H); 7.55 (d, 1H); 7.86-7.96 (3H); 12.25 (br s, 1H).
The following compounds were prepared analogously:
Yield: 16%
1H NMR (D6-DMSO): 3.32 (s, 3H); 3.74 (t, 2H); 4.44 (t, 2H); 7.33 (d, 1H); 7.56 (d, 1H); 7.92-8.01 (3H); 12.14 (s, 1H).
Yield: 20%
1H NMR (D6-DMSO): 3.35 (s, 3H); 3.72 (t, 2H); 4.18 (t, 2H); 5.53 (s, 2H); 6.69 (d, 1H); 7.19 (d, 1H); 7.51 (d, 1H); 7.57 (dd, 1H); 7.64 (d, 1H); 12.14 (s, 1H).
Yield: 11%
1H-NMR (D6-DMSO): 1.05 (t, 3H); 1.7 (m, 2H); 4.0 (t, 2H); 5.5 (br, 2H), 6.7 (d, 1H); 7.2 (d, 1H); 7.5 (d, 1H); 7.55 (d, 1H); 7.6 (s, 1H), 12.15 (br, 1H).
4-Amino-3-chloro-benzoic acid methyl ester (21.6 mmol) was saponified in EtOH (25 ml) and NaOH (1M, 25 ml) at reflux for 2 h. The organic solvent was evaporated and pH adjusted to 4. The product was removed by filtration, washed with water and dried in vacuo.
Yield: 92%
1H NMR (D6-DMSO): 6.15 (s, 2H); 6.79 (d, 1H); 7.59 (dd, 1H); 7.71 (d, 1H); 12.37 (br s, 1H).
4-Amino-3-chloro-benzoic acid (19.8 mmol) was dissolved in DMF (10 mL) and 1,2-dichloroethane (80 mL). DIPEA (19.8 mmol), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (19.8 mmol), 1-hydroxybenzotriazole (19.8 mmol) and 2-aminothiazole (19.8 mmol) was added, and the reaction mixture was stirred at 60° C. over night. The volume was reduced in vacuo, and water (60 mL) was added. The mixture was extracted with ethyl acetate, the organic phase was washed with NH4Cl (aq., sat.), dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using gradient elution (heptane/ethyl acetate).
Yield: 42% (39% overall)
1H NMR (D6-DMSO): 6.19 (s, 2H); 6.83 (d, 1H); 7.21 (d, 1H); 7.52 (d, 1H); 7.83 (dd, 1H); 8.07 (d, 1H), 12.24 (br s, 1H).
4-Amino-benzoic acid (100 mmol) was dissolved in DMF (50 mL) and N-bromo-succinimide (100 mmol) was added. Stirred at ambient temperature for 18 h, the reaction mixture was then poured into water (100 mL). The product was removed by filtration, washed with water and dried in vacuo.
Yield: 70%
1H NMR (D6-DMSO): 6.10 (s, 2H); 6.78 (d, 1H); 7.63 (dd, 1H); 7.89 (d, 1H); 12.39 (br s, 1H).
4-Amino-3-bromo-benzoic acid (18.5 mmol) was dissolved in DMF (10 mL) and 1,2-dichloroethane (80 mL). DIPEA (18.5 mmol), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (18.5 mmol), 1-hydroxybenzotriazole (18.5 mmol) and 2-aminothiazole (18.5 mmol) was added and the reaction mixture was stirred at 60° C. over night. The volume was reduced in vacuo, and water (60 mL) was added. The mixture was extracted with ethyl acetate, the organic phase was washed with NH4Cl (aq., sat.), dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using gradient elution (heptane/ethyl acetate).
Yield: 33% (23% overall)
1H NMR (D6-DMSO): 6.14 (s, 2H); 6.82 (d, 1H); 7.21 (d, 1H); 7.51 (d, 1H); 7.86 (dd, 1H); 8.22 (d, 1H); 12.24 (br s, 1H).
4-Amino-5-chloro-2-methoxy-benzoic acid (19.8 mmol)) was dissolved in DMF (10 mL) and 1,2-dichloroethane (80 mL). DIPEA (19.8 mmol), 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride (19.8 mmol), 1-hydroxybenzotriazole (19.8 mmol) and 2-amino thiazole (19.8 mmol) was added and the reaction mixture was stirred at 60° C. over night. The volume was reduced in vacuo, and water (60 mL) was added. The mixture was extracted with ethyl acetate, the organic phase was washed with NH4Cl (aq., sat.), dried over MgSO4, filtered and evaporated. The crude product was re-crystallized from ethyl acetate.
Yield: 32%
1H NMR (D6-DMSO): 3.94 (s, 3H); 6.30 (s, 2H); 6.56 (s, 1H); 7.23 (d, 1H); 7.49 (d, 1H); 7.76 (s, 1H); 11.05 (br s, 1H).
The following compounds were prepared analogously:
Yield: 33%
1H NMR (D6-DMSO): 4.34 (m, 2H); 4.49 (m, 2H); 5.68 (s, 2H); 5.37 (d, 1H); 7.21 (d, 1H); 7.34 (d, 1H); 7.48 (d, 1H); 10.94 (br s, 1H).
Yield: 27%
LC/MS (m/z) 256 (MH+); RT=1.9 (method A); purity (UV, ELSD): 94%; 99%.
Yield: 71%
LC/MS (m/z) 304 (MH+); RT=2.3 (method A); purity (UV, ELSD): 64%; 98%.
Yield: 1.1%
LC/MS (m/z) 268 (MH+); RT=2.2 (method A); purity (UV, ELSD): 97%; 99%.
Yield: 69%
LC/MS (m/z) 248 (MH+); RT=1.8 (method A); purity (UV, ELSD): 85%; 99%.
The starting material: 4-amino-3-trifluoromethyl-benzoic acid was prepared according to literature procedures: Krüger et al. Arzneim. Forsch.; 34; 11a; 1984; 1612-1624.
Yield: 14%
The starting material: 4-amino-3-chloro-5-trifluoromethyl-benzoic acid was prepared according to literature procedures: Krüger et al. Arzneim. Forsch.; 34; 11a; 1984; 1612-1624.
Yield: 20%
LC/MS (m/z) 322 (MH+); RT=2.6 (method A); purity (UV, ELSD): 97%; 99%.
2-Bromo-4-nitro-toluene (50 mmol), phenyl boronic acid (55 mmol), Pd(PPh3)2Cl2 (2.5 mmol) and K2CO3 (160 mmol) was combined in a flask and refluxed in a mixture of ethylene glycol dimethyl ether (50 mL) and water (40 mL) under an Argon atmosphere for 18 h. The dark mixture was diluted with water (200 mL) and extracted with ethyl acetate. The organic phase was dried over Na2CO3, filtered and evaporated to yield a brown oil, which was triturated with water. The crude product precipitated and was removed by filtration and re-crystallized from MeOH.
Yield: 96%
1H NMR (D6-DMSO): 2.35 (s, 3H); 7.32-8.20 (8H).
2-Methyl-5-nitro-biphenyl (48 mmol) was suspended in pyridine (100 mL) and water (150 mL), and KMnO4 (239 mmol) was added portion wise over a period of one hour. The mixture was refluxed for 5 h. The reaction mixture was cooled, and MnO2 was filtered off. HCl (conc.) was added to the filtrate until the product precipitated, the product was removed by filtration, washed with water and dried in vacuo.
Yield: 85%
1H NMR (D6-DMSO): 7.37-8.39 (8H).
5-Nitro-biphenyl-2-carboxylic acid (25 mmol) was suspended in 1,2-dichloroethane (100 mL) and two drops of DMF was added followed by drop wise addition of oxalylchloride (2M solution in dichloromethane) (40 mmol). The mixture turned homogeneous and was stirred at ambient temperature for 1 h. Evaporated to dryness, then re-dissolved in 1,2-dichloroethane (20 mL) and added to a suspension of 2-amino thiazole (25 mmol) and pyridine (30 mmol) in 1,2-dichloroethane (50 mL). Stirred over night at ambient temperature, then the mixture was evaporated to dryness and triturated with water. The crude product was filtered off and boiled in MeOH. The white solid was filtered off, and dried in vacuo.
Yield: 73%
1H NMR (D6-DMSO): 7.27 (d, 1H); 7.32-7.53 (7H); 7.92 (d, 1H); 8.23 (m, 1H); 8.32 (m, 1H); 12.75 (s, 1H).
5-Nitro-biphenyl-2-carboxylic acid thiazol-2-ylamide (18.1 mmol) was suspended in MeOH (50 mL) and glacial acetic acid (10 mL), and Zn (s) (50 mmol) was added. The mixture turned homogenous after a few minutes, and stirring was continued for 24 h. The reaction mixture was filtered and evaporated to dryness, then water (200 mL) was added. The crude product was removed by filtration, washed with water and dried.
Yield: 89% (53% overall)
1H NMR (D6-DMSO): 5.71 (s, 2H); 6.55 (m, 2H); 7.12 (d, 1H); 7.20-7.41 (7H); 11.52 (br s, 1H).
4-Amino-benzoic acid (100 mmol) was dissolved in DMF (100 mL) and N-bromo succinimide (100 mmol) was added portion wise. The orange reaction mixture was stirred over night at ambient temperature, then poured into water. The product was collected by filtration and re-crystallized from MeOH.
Yield: 65%
1H NMR (D6-DMSO): 6.09 (s, 2H); 6.81 (d, 1H); 7.13 (dd, 1H); 7.89 (d, 1H); 12.37 (br, 1H).
4-Amino-3-bromo-benzoic acid (65 mmol), phenyl boronic acid (70 mmol), Pd(PPh3)2Cl2 (3.2 mmol) and K2CO3 (140 mmol) was combined in a flask and refluxed in a mixture of ethylene glycol dimethyl ether (75 mL) and water (75 mL) under an argon atmosphere for 18 h. The organic solvent was removed in vacuo and pH was adjusted to 4. The crude product was removed by filtration, re-dissolved in ethyl acetate, and passed through a silica plug to remove any Pd-residues. The filtrate was evaporated to dryness and re-crystallized from ethyl acetate/heptane to give off-white crystals.
Yield: 47%
NMR (D6-DMSO): 5.56 (s, 2H); 6.78 (d, 1H); 7.30-7.75 (8H); 12.09 (br, 1H).
6-Amino-biphenyl-3-carboxylic acid (19 mmol) was dissolved in DMF (10 mL) and 1,2-dichloroethane (80 mL). DIPEA (19 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (19 mmol), 1-hydroxybenzothiazole (19 mmol) and 2-amino thiazole (19 mmol) was added, and the reaction mixture was stirred at 50° C. over night. The volume was reduced in vacuo, and water (60 mL) was added. The mixture was extracted with ethyl acetate, the organic phase was washed with NH4Cl (aq., sat.), dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using gradient elution (ethyl acetate/heptane).
Yield: 26% (8% overall)
NMR (D6-DMSO): 5.64 (s, 2H); 6.82 (d, 1H); 7.19 (d, 1H); 7.34-7.53 (6H); 7.83 (d, 1H); 7.88 (d, 1H); 12.21 (s, 1H).
2,3-Dihydro-1H-indole-5-carboxylic acid (12.4 mmol) was dissolved in DMF (5 mL) and 1,2-dichloroethane (40 mL). DIPEA (12.4 mmol), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (12.4 mmol), 1-hydroxybenzotriazole (12.4 mmol) and 2-aminothiazole (12.4 mmol) was added. Stirred at 50° C. for 48 h. HCl (2M) (12.4 mmol) was added to the reaction mixture followed by water (30 mL). This gave a heavy precipitation which was removed by filtration. The solid was washed with water and 1,2-dichloroethane and dried.
Yield: 56%
1H NMR (D6-DMSO): 6.60 (s, 1H); 7.26 (d, 1H); 7.44-7.54 (2H); 7.56 (d, 1H); 7.86 (d, 1H); 8.45 (s, 1H); 11.51 (br s, 1H).
1H-Indole-5-carboxylic acid thiazol-2-ylamide (7 mmol) was dissolved in glacial acetic acid (40 mL) and sodium cyanoborohydride (14 mmol) was added portion wise. After stirring at room temperature for 18 h the reaction was incomplete and another 14 mmol of sodium cyanoborohydride was added. Stirred at room temperature for 24 h. The reaction mixture was poured into ice water (200 mL). The pH of the mixture was adjusted to 10 with NaOH (conc.), and the aqueous phase was extracted with ethyl acetate. The organic extracts were combined, dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica using a gradient of ethyl acetate/heptane (10:90 to 70:30) as eluent.
Yield: 32% (18% overall)
1H NMR (D6-DMSO): 2.98 (t, 2H); 3.57 (t, 2H); 6.35 (br s, 1H); 6.48 (d, 1H); 7.17 (d, 1H); 7.49 (d, 1H); 7.75-7.82 (3H); 12.02 (br s, 1H).
1,2,3,4-Tetrahydro-quinoline (100 mmol) was dissolved in 1,2-dichloroethane (100 mL) and acetic anhydride (102 mmol) was added. Stirred at room temperature for 4 h, then the solvent was removed by evaporation and the residue was dissolved in ethyl acetate and water. The aqueous phase was neutralized with NaOH (2M), the organics were separated, dried over MgSO4, filtered and evaporated. The crude was used directly in the next reaction
Crude 1-(3,4-dihydro-2H-quinolin-1-yl)-ethanone was dissolved in DMF (60 mL), and N-bromo succinimide (100 mmol) was added portion wise. Stirred at room temperature for 3 h, then the reaction mixture was poured into water (150 mL) and extracted with ethyl acetate. The organic phase was washed with NH4Cl (sat.), dried over MgSO4, filtered and evaporated.
Yield: 96%
1H NMR (D6-DMSO): 1.85 (m, 2H); 2.16 (s, 3H); 2.70 (m, 2H); 3.66 (m, 2H); 7.25-7.60 (3H).
1-(6-Bromo-3,4-dihydro-2H-quinolin-1-yl)-ethanone (96 mmol) was dissolved in DMF (60 mL) and CuCN (200 mmol) was added. The reaction mixture was refluxed for 18 h, then cooled and poured into water (400 mL). Aq. NH3 (sat.) (100 mL) was added, and the mixture was stirred vigorously until it had turned blue. The product had precipitated and was removed by filtration, washed with water and dried.
Yield: 53%
1H NMR (D6-DMSO): 1.78 (m, 2H); 2.67 (m, 2H); 3.23 (m, 2H); 6.43 (d, 1H); 7.41-7.47 (2H).
m/z: 201 (MH+) 1-Acetyl-1,2,3,4-tetrahydro-quinoline-6-carbonitrile (50 mmol) was refluxed in HCl (8M) (150 mL) for 18 h. The mixture was cooled and pH adjusted to approx. 3 with NaOH. The product precipitated and was removed by filtration, washed with water and dried.
Yield: 61%
1H NMR (D6-DMSO): 1.79 (m, 2H); 2.68 (m, 2H); 3.23 (m, 2H); 5.45 (br, 1H); 6.48 (d, 1H); 7.45-7.50 (2H).
1,2,3,4-Tetrahydro-quinoline-6-carboxylic acid (17 mmol) was dissolved in DMF (5 mL) and 1,2-dichloroethane (15 mL) was added, followed by DIPEA (17 mmol.), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (17 mmol), 101-hydroxybenzotriazole (17 mmol) and 2-aminothiazole (17 mmol). Stirred at 60° C. for 96 h, then HCl (17 mmol) and water (20 mL) was added. The organic solvent was removed by evaporation, and the aqueous phase was extracted with ethyl acetate. The organics were dried over MgSO4, filtered and evaporated. The crude product was purified by flash chromatography on silica with gradient (heptane/ethyl acetate) elution.
Yield: 10% (3% overall)
1H NMR (D6-DMSO): 1.80 (m, 2H); 2.70 (m, 2H); 3.24 (m, 2H); 6.46 (d, 1H); 6.56 (s, 1H); 7.16 (d, 1H); 7.49 (d, 1H); 7.65-7.72 (2H).
3-Hydroxy-4-nitro-benzoic acid (100 mmol) was refluxed in HCl (6M) (90 mL) and MeOH (160 mL) over night, the mixture was cooled and poured into water (500 mL). The product was removed by filtration, washed with water and dried.
The crude product was used directly in the next reaction.
Crude 3-hydroxy-4-nitro-benzoic acid methyl ester and triphenylphosphine (100 mmol) was dissolved in dry THF (120 mL) and cooled to 0° C. Di-ethyl-azo-dicarboxylate (110 mmol) was added followed by 2-chloroethanol (110 mmol). The reaction mixture was allowed to come to room temperature and was stirred at this temperature over night. The solvent was removed by evaporation and the residue was re-dissolved in MeOH (80 mL), and water (20 mL). The product precipitated and was removed by filtration, washed with water and dried.
Yield: 60%
1H NMR (D6-DMSO): 3.91 (s, 3H); 3.96 (t, 2H); 4.54 (t, 2H); 7.70 (dd, 1H); 7.81 (d, 1H); 8.01 (d, 1H).
3-(2-Chloro-ethoxy)-4-nitro-benzoic acid methyl ester (58 mmol) was suspended in abs. EtOH (200 mL), and ethyl acetate (100 mL), glacial acetic acid (10 mL) and 10% Pd/C (1 g) was added. The mixture was hydrogenated under 3 bar H2 for 3 days. The reaction mixture was filtered and evaporated.
The crude product was used directly in the next reaction.
4-Amino-3-(2-chloro-ethoxy)-benzoic acid methyl ester (58 mmol) was dissolved in DMF (150 mL) and K2CO3 (60 mmol) was added. Stirred at 100° C. for 4 days. The reaction mixture was poured into water (500 mL) and extracted with ethyl acetate. The organic phases were washed with NH4Cl (sat.), dried over MgSO4, filtered and evaporated. The crude product was used directly in the next reaction.
3,4-Dihydro-2H-benzo[1,4]oxazine-7-carboxylic acid methyl ester (52 mmol) was dissolved in MeOH (20 mL) and NaOH (2M) (20 mL) and stirred at 60° C. for 48 h. The organic solvent was removed by evaporation, and the aqueous phase acidified with HCl (4M). The organic products separated as an oil, and were extracted with ethyl acetate. The organic extract was dried over MgSO4, filtered and evaporated. The crude was purified by flash chromatography on silica with gradient elution (heptane/ethyl acetate).
Yield: 31%
1H NMR (D6-DMSO): 3.33 (t, 2H); 4.10 (t, 2H); 6.51 (d, 1H); 7.16 (d, 1H); 7.30 (dd, 1H).
3,4-Dihydro-2H-benzo[1,4]oxazine-7-carboxylic acid (16 mmol) was dissolved in DMF (5 mL) and 1,2-dichloroethane (15 mL) was added, followed by DIPEA (16 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (16 mmol), 1-hydroxybenzotriazole (16 mmol) and 2-aminothiazole (16 mmol). Stirred at 60° C. for 96 h, then HCl (16 mmol) and water (20 mL) was added. The organic solvent was removed by evaporation, and the aqueous phase was extracted with ethyl acetate. The product precipitated and was filtered, washed with water and dried. The crude product was re-crystallized from EtOH/water.
Yield: 40% (6% overall)
1H NMR (D6-DMSO): 3.58 (t, 2H); 4.35 (t, 2H); 6.82 (d, 1H); 6.89 (br s, 1H); 7.41 (d, 1H); 7.69 (d, 1H); 7.72 (d, 1H); 7.76 (dd, 1H).
2-Amino thiazole (21 mmol) and pyridine (21 mmol) were suspended in 1,2-dichloroethane (100 mL) and a suspension of methyl 4-chlorocarbonylbenzoate (25 mmol) in 1,2-dichloroethane (300 mL) was added portion wise. Stirred at 50° C. over night, then the solvent was removed under reduced pressure. The remaining solids were re-suspended in ethyl acetate and NaHCO3 (sat.) and then filtered, washed with ethyl acetate and dried in vacuo.
Yield: 79%
1H NMR (D6-DMSO): 3.90 (s, 3H); 7.31 (d, 1H); 7.59 (d, 1H); 8.09 (d, 2H); 8,20 (d, 2H); 12.83 (br s, 1H).
N-Thiazol-2-yl-terephthalamic acid methyl ester (16 mmol) was dissolved in THF (100 mL) and NaOH (2M, aq.) (100 mL) and stirred at room temperature for 4 h. The organic solvent was removed by evaporation under reduced pressure and the aqueous phase acidified with HCl (2M, aq.). The precipitated product was removed by filtration, washed with water and dried in vacuo.
Yield: 71% (56% overall)
1H NMR (D6-DMSO): 7.33 (d, 1H); 7.59 (d, 1H); 8.07 (d, 2H); 8.19 (d, 2H); 13.08 (br, 1H).
2-Amino-5-chlorothiazole hydrochloride (100 mmol) was suspended in 1,2-dichloroethane (60 mL) and pyridine (4.7 mmol) was added. A suspension of 4-nitro benzoic acid chloride (43.8 mmol) in 1,2-dichloroethane (150 mL). The reaction mixture was stirred at 50° C. over night. The reaction mixture was cooled to room temperature ant washed with NaHCO3 (sat) (100 mL), water (100 mL) and NaCl (100 mL). The solvent was removed by evaporation under reduced pressure. The crude product was recrystallised form EtOH.
Yield: 29%
1H-NMR (D6-DMSO): 7.65 (s, 1H); 8.3 (d, 2H); 8.4 (d, 2H); 13.25 (br, 1H).
N-(5-Chloro-thiazol-2-yl)-4-nitro-benzamide (12.9 mmol) was suspended in MeOH (40 mL) and glacial acetic acid (40 mL), and Zn (s) (51.5 mmol) was added. The mixture was stirred at 70° C. for 48 h. The reaction mixture was evaporated to dryness. Water (200 mL) and concentrated hydrochloric acid (5 mL) were added. The mixture was filtered and the solvent was removed by evaporation. The crude product was recrystallised from EtOH/water.
Yield: 54%
1H-NMR (D6-DMSO): 6.04 (s, 2H); 6.6 (d, 2H); 7.5 (s, 1H); 7.85 (d, 2H); 12.4 (br).
3-Bromomethyl-4-nitro-benzoic acid ethyl ester (3.4 mmol) (Can be prepared according to literature procedures: Damen et al.; Bioorg. Med. Chem.; EN; 10; 1; 2002; 71-78) was suspended in MeOH (20 mL). NaOMe (5.4 M in MeOH, 0.77 mL) was added slowly at 0° C. The reaction mixture was stirred 1 h at 0° C., then overnight at room temperature. The solvent was removed under reduced pressure. Ethyl acetate (100 mL) was added and the organic phase was washed with water (50 mL) and brine (50 mL). The organic phase was dried over MgSO4 filtered and evaporated.
Yield: 71%
1H-NMR (D6-DMSO): 3.4 (s, 3H); 3.9 (s, 3H); 4.8 (s, 2H); 8.1 (d, 1H); 8.2 (d, 1H); 8.3 (s, 1H).
3-Methoxymethyl-4-nitro-benzoic acid methyl ester (2.6 mmol) was dissolved in MeOH (10 mL) and NaOH (2M, 10 mL) was added. The reaction mixture was stirred at 25° C. overnight. pH was adjusted with HCl to pH=3. 3-Methoxymethyl-4-nitrobenzoic acid was filtered off and washed with water.
Yield: 45%
1H-NMR (D6-DMSO): 3.4 (s, 3H); 4.8 (s, 2H); 8.05 (d, 1H); 8.51 (d, 1H); 8.75 (s, 1H).
3-Methoxymethyl-4-nitro-benzoic acid (1.6 mmol) was suspended in 1,2-dichloroethane (10 mL) and dimethylformamide (DMF) (0.1 mL) under an argon atmosphere. Oxalylchloride (2M in dichloromethane, 1.3 mL) was added slowly to the stirred suspension. After stirring at room temperature for 1 h, the solvent was removed by evaporation under reduced pressure, and the reaction mixture was re-dissolved in 1,2-dichloroethane (7 mL). A suspension of 2-amino thiazole (1.3 mmol) and pyridine (0.13 mmol) in 1,2-dichloroethane (5 mL) was added portion wise. The reaction mixture was stirred at 50° C. for 48 h. The solvent was removed under reduced pressure. The crude was re-crystallized from EtOH/water. The product contained small amounts of starting material. Used without further purification.
LC/MS (m/z) 294 (MH+); RT=2.4 (method A); purity (UV, ELSD): 74%; 92%.
3-Methoxymethyl-4-nitro-N-thiazol-2-yl-benzamide (0.88 mmol) was suspended in abs. EtOH (9 mL). Ethyl acetate (4.5 mL) and glacial acetic acid (1.5 mL) was added followed by 10% Pd/C (0.5 g). The mixture was hydrogenated for 72 h at 3 bar H2. The hydrogenation mixture was filtered, and the solvent was removed under reduced pressure. The crude product was added NaOH (1M) and extracted with ethyl acetate. The organic phase was washed with water and the solvent was removed under reduced pressure. Purified by preparative HPLC-MS.
Yield: 17%
1H-NMR (D6-DMSO): 3.3 (s, 3H); 4.4 (s, 2H); 6.7 (d, 1H); 7.2 (d, 1H); 7.5 (d, 1H); 7.85 (d, 1H); 7.9 (s, 1H), 12.1 (br, 1H).
Preparation of the Compounds of the Invention
200 μL of a 0.43 M stock solution of butanoic acid in DMF containing 6 mmol DIPEA per mmol butanoic acid was mixed with 100 μL of a 0.86 M stock solution of O-(7-azabenzotriazole-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU) in DMF. The mixture was allowed to react for 10 min. at ambient temperature, then 100 μL of a 0.43 M stock solution of 4-amino-N-thiazol-2-yl-benzamide in DMF was added. The resulting mixture was incubated for 18 h at ambient temperature. Purification was performed by preparative HPLC-MS.
1H NMR (D6-DMSO): 0.92 (t, 3H); 1.63 (m, 2H); 2.34 (t, 2H); 7.26 (d, 1H); 7.55 (d, 1H); 7.74 (d, 2H); 8.06 (d, 2H); 10.20 (s, 1H); 12.47 (br s, 1H).
LC/MS (m/z) 290 (MH+); RT=2.12 (method B); purity (UV, ELSD): 98%, 100%.
The following compounds were prepared analogously:
LC/MS (m/z) 362 (MH+); RT=2,06 (method A); purity (UV, ELSD): 75%; 95%.
LC/MS (m/z) 318 (MH+); RT=2,45 (method A); purity (UV, ELSD): 100%; 94%.
LC/MS (m/z) 332 (MH+); RT=2,56 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 358 (MH+); RT=2,90 (method A); purity (UV, ELSD): 100%; 93%.
LC/MS (m/z) 348 (MH+); RT=2,65 (method A); purity (UV, ELSD): 94%; 98%.
LC/MS (m/z) 389 (MH+); RT=3,17 (method A); purity (UV, ELSD): 80%; 83%.
LC/MS (m/z) 344 (MH+); RT=2,08 (method A); purity (UV, ELSD): 84%; 91%.
LC/MS (m/z) 348 (MH+); RT=2,77 (method A); purity (UV, ELSD): 80%; 100%.
LC/MS (m/z) 380 (MH+); RT=2,70 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 344 (MH+); RT=2,71 (method A); purity (UV, ELSD): 100%; 91%.
LC/MS (m/z) 356 (MH+); RT=2,78 (method A); purity (UV, ELSD): 97%; 93%.
LC/MS (m/z) 346 (MH+); RT=2,59 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 397 (MH+); RT=3,12 (method A); purity (UV, ELSD): 80%; 96%.
LC/MS (m/z) 410 (MH+); RT=2,44 (method A); purity (UV, ELSD): 87%; 98%.
LC/MS (m/z) 350 (MH+); RT=1,90 (method A); purity (UV, ELSD): 96%; 88%.
LC/MS (m/z) 372 (MH+); RT=2,66 (method A); purity (UV, ELSD): 84%; 95%.
LC/MS (m/z) 324 (MH+); RT=2,33 (method A); purity (UV, ELSD): 97%; 99%.
LC/MS (m/z) 330 (MH+); RT=2,33 (method A); purity (UV, ELSD): 100%; 99%.
LC/MS (m/z) 352 (MH+); RT=2,50 (method A); purity (UV, ELSD): 99%; 91%.
LC/MS (m/z) 344 (MH+); RT=2,28 (method A); purity (UV, ELSD): 94%; 85%.
200 μL of a 0.2 M stock solution of 4-amino-3-methyl-N-thiazol-2-yl-benzamide in 1,2-dichloroethane/DMF, containing 1.2 mmol pyridine per mmol 4-amino-3-methyl-N-thiazol-2-yl-benzamide, was added 0.05 mmol of cyclopentyl-acetyl chloride. The reaction mixture was incubated at ambient temperature for 2 h. Purification was performed by preparative HPLC-MS.
1H NMR (D6-DMSO): 1.23 (m, 2H); 1.53 (m, 2H); 1.63 (m, 2H); 1.78 (m, 2H); 2.25 (m, 1H); 2.40 (d, 2H); 2.90 (s, 3H); 7.27 (d, 1H); 7.56 (d, 1H); 7.68 (d, 1H); 7.90 (dd, 1H); 7.98 (d, 1H); 9.38 (s, 1H); 12.49 (br).
LC/MS (m/z) 344 (MH+); RT=2.66 (method A); purity (UV, ELSD): 95%, 98%.
The following compounds were prepared analogously:
LC/MS (m/z) 318 (MH+); RT=2,54 (method A); purity (UV, ELSD): 99%; 99%.
LC/MS (m/z) 332 (MH+); RT=2,44 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 348 (MH+); RT=2,63 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 339 (MH+); RT=2,60 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 397 (MH+); RT=2,85 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 304 (MH+); RT=2,22 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 383 (MH+); RT=2,64 (method A); purity (UV, ELSD): 88%; 96%.
LC/MS (m/z) 330 (MH+); RT=2,42 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 318 (MH+); RT=2,24 (method A); purity (UV, ELSD): 100%; 97%.
LC/MS (m/z) 351 (MH+); RT=2,73 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 373 (MH+); RT=2,81 (method A); purity (UV, ELSD): 89%; 100%.
LC/MS (m/z) 395 (MH+); RT=2,79 (method A); purity (UV, ELSD): 99%; 99%.
LC/MS (m/z) 316 (MH+); RT=2,32 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 330 (MH+); RT=2,34 (method A); purity (UV, ELSD): 100%; 97%.
LC/MS (m/z) 423 (MH+); RT=3,20 (method A); purity (UV, ELSD): 90%; 99%.
LC/MS (m/z) 320 (MH+); RT=2,20 (method A); purity (UV, ELSD): 100%; 97%.
LC/MS (m/z) 376 (MH+); RT=2,68 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 369 (MH+); RT=2,43 (method A); purity (UV, ELSD): 89%; 100%.
LC/MS (m/z) 334 (MH+); RT=2,44 (method A); purity (UV, ELSD): 100%; 95%.
LC/MS (m/z) 344 (MH+); RT=2,71 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 334 (MH+); RT=2,40 (method A); purity (UV, ELSD): 98%; 98%.
LC/MS (m/z) 346 (MH+); RT=2,52 (method A); purity (UV, ELSD): 100%; 95%.
LC/MS (m/z) 417 (MH+); RT=2,86 (method A); purity (UV, ELSD): 96%; 100%.
LC/MS (m/z) 353 (MH+); RT=2,82 (method A); purity (UV, ELSD): 95%; 97%.
LC/MS (m/z) 389 (MH+); RT=3,32 (method A); purity (UV, ELSD): 87%; 100%.
LC/MS (m/z) 376 (MH+); RT=3,24 (method A); purity (UV, ELSD): 84%; 99%.
LC/MS (m/z) 348 (MH+); RT=2,75 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 362 (MH+); RT=3,02 (method A); purity (UV, ELSD): 87%; 100%.
LC/MS (m/z) 322 (MH+); RT=2,41 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 308 (MH+); RT=2,19 (method A); purity (UV, ELSD): 73%; 84%.
LC/MS (m/z) 348 (MH+); RT=2,82 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 356 (MH+); RT=2,57 (method A); purity (UV, ELSD): 95%; 100%.
LC/MS (m/z) 362 (MH+); RT=2,90 (method A); purity (UV, ELSD): 88%; 99%.
LC/MS (m/z) 334 (MH+); RT=2,53 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 392 (MH+); RT=2,85 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 356 (MH+); RT=2,69 (method A); purity (UV, ELSD): 94%; 100%.
LC/MS (m/z) 378 (MH+); RT=3,25 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 405 (MH+); RT=2,97 (method A); purity (UV, ELSD): 95%; 100%.
LC/MS (m/z) 380 (MH+); RT=2,65 (method A); purity (UV, ELSD): 96%; 100%.
LC/MS (m/z) 336 (MH+); RT=2,74 (method A); purity (UV, ELSD): 94%; 100%.
LC/MS (m/z) 332 (MH+); RT=2,66 (method A); purity (UV, ELSD): 94%; 87%.
LC/MS (m/z) 383 (MH+); RT=3,14 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 332 (MH+); RT=2,54 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 369 (MH+); RT=2,93 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 352 (MH+); RT=2,53 (method A); purity (UV, ELSD): 95%; 100%.
LC/MS (m/z) 358 (MH+); RT=2,80 (method A); purity (UV, ELSD): 97%; 95%.
LC/MS (m/z) 403 (MH+); RT=3,15 (method A); purity (UV, ELSD): 86%; 99%.
LC/MS (m/z) 344 (MH+); RT=2,58 (method A); purity (UV, ELSD): 99%; 98%.
LC/MS (m/z) 344 (MH+); RT=2,79 (method A); purity (UV, ELSD): 99%; 97%.
LC/MS (m/z) 332 (MH+); RT=2,62 (method A); purity (UV, ELSD): 92%; 92%.
LC/MS (m/z) 373 (MH+); RT=2,99 (method B); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 378 (MH+); RT=2,66 (method A); purity (UV, ELSD): 81%; 99%.
LC/MS (m/z) 419 (MH+); RT=3,80 (method A); purity (UV, ELSD): 83%; 98%.
LC/MS (m/z) 360 (MH+); RT=3,04 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 344 (MH+); RT=2,79 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 360 (MH+); RT=2,79 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 358 (MH+); RT=2,72 (method A); purity (UV, ELSD): 100%; 93%.
LC/MS (m/z) 382 (MH+); RT=2,72 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 379 (MH+); RT=3,14 (method A); purity (UV, ELSD): 84%; 94%.
LC/MS (m/z) 382 (MH+); RT=2,39 (method A); purity (UV, ELSD): 94%; 100%.
LC/MS (m/z) 409 (MH+); RT=2,95 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 325 (MH+); RT=2,39 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 395 (MH+); RT=3,27 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 381 (MH+); RT=3,09 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 360 (MH+); RT=2,73 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 384 (MH+); RT=2,55 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 368 (MH+); RT=2,58 (method A); purity (UV, ELSD): 87%; 99%.
LC/MS (m/z) 358 (MH+); RT=2,33 (method A); purity (UV, ELSD): 88%; 100%.
LC/MS (m/z) 365 (MH+); RT=2,92 (method A); purity (UV, ELSD): 98%; 97%.
LC/MS (m/z) 368 (MH+); RT=2,35 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 304 (MH+); RT=2,02 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 373 (MH+); RT=2,43 (method A); purity (UV, ELSD): 95%; 100%.
LC/MS (m/z) 407 (MH+); RT=2,75 (method A); purity (UV, ELSD): 96%; 100%.
LC/MS (m/z) 389 (MH+); RT=2,56 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 348 (MH+); RT=2,58 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 368 (MH+); RT=2,52 (method A); purity (UV, ELSD): 83%; 100%.
LC/MS (m/z) 366 (MH+); RT=2,50 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 348 (MH+); RT=2,68 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 375 (MH+); RT=3,06 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 412 (MH+); RT=2,86 (method A); purity (UV, ELSD): 79%; 95%.
LC/MS (m/z) 334 (MH+); RT=2,61 (method A); purity (UV, ELSD): 96%; 100%.
LC/MS (m/z) 368 (MH+); RT=2,74 (method A); purity (UV, ELSD): 98%; 99%.
LC/MS (m/z) 360 (MH+); RT=2,46 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 354 (MH+); RT=2,40 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 388 (MH+); RT=2,78 (method A); purity (UV, ELSD): 99%; 100%.
LC/MS (m/z) 407 (MH+); RT=3,08 (method A); purity (UV, ELSD): 94%; 100%.
LC/MS (m/z) 405 (MH+); RT=2,74 (method A); purity (UV, ELSD): 86%; 96%.
LC/MS (m/z) 336 (MH+); RT=2,66 (method A); purity (UV, ELSD): 97%; 98%.
LC/MS (m/z) 373 (MH+); RT=2,60 (method A); purity (UV, ELSD): 90%; 97%.
LC/MS (m/z) 342 (MH+); RT=2,34 (method A); purity (UV, ELSD): 97%; 100%.
LC/MS (m/z) 354 (MH+); RT=2,58 (method A); purity (UV, ELSD): 96%; 97%.
LC/MS (m/z) 394 (MH+); RT=2,77 (method A); purity (UV, ELSD): 100%; 99%.
LC/MS (m/z) 395 (MH+); RT=2,87 (method A); purity (UV, ELSD): 98%; 100%.
LC/MS (m/z) 352 (MH+); RT=3,1 (method A); purity (UV, ELSD): 100%; 100%.
LC/MS (m/z) 338 (MH+); RT=2,9 (method A); purity (UV, ELSD): 95%; 98%.
LC/MS (m/z) 336 (MH+); RT=2,7 (method A); purity (UV, ELSD): 88%; 97%.
LC/MS (m/z) 324 (MH+); RT=2,7 (method A); purity (UV, ELSD): 99%; 99%.
LC/MS (m/z) 358 (MH+); RT=2,9 (method A); purity (UV, ELSD): 97%; 99%.
LC/MS (m/z) 376 (MH+); RT=2,7 (method A); purity (UV, ELSD): 97%; 72%.
LC/MS (m/z) 402 (MH+); RT=3,1 (method A); purity (UV, ELSD): 98%; 99%.
LC/MS (m/z) 362 (MH+); RT=2.6 (method A); purity (UV, ELSD): 86%; 99%.
LC/MS (m/z) 376 (MH+); RT=3.3 (method A); purity (UV, ELSD): 99%; 97%.
4-Amino-3-chloro-5-methyl-N-thiazol-2-yl-benzamide (0.06 mmol) was dissolved in 1,2-dichloroethane (0.75 mL). Pyridine (5.8 μL) and 3,3-Dimethyl-butyryl chloride (10 μL) were added. The reaction mixture was heated to 130° C. for 2 h in Personal chemistry microwave oven?. The product was filtered off and dried.
LC/MS (m/z) 366.1 (MH+); RT=2,6 (method A); purity (UV, ELSD): 98%; 99%.
LC/MS (m/z) 354.0 (MH+); RT=2,5 (method A); purity (UV, ELSD): 91%; 99%.
LC/MS (m/z) 345.9 (MH+); RT=2,5 (method A); purity (UV, ELSD): 95%; 99%.
LC/MS (m/z) 386.2 (MH+); RT=2,9 (method A); purity (UV, ELSD): 95%; 99%.
LC/MS (m/z) 420.3 (MH+); RT=2,9 (method A); purity (UV, ELSD): 95%; 98%.
N-Thiazol-2-yl-terephthalamic acid (2 mmol) was dissolved in 1,2-dichloroethane (10 mL) and DMF (0.5 mL). DIPEA (2 mmol), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (2 mmol), 1-hydroxybenzotriazole (2 mmol) and 2,2-dimethyl-propylamine (2.4 mmol) was added. Stirred over night at room temperature, then 2.4 mmol HCl (2M) was added along with water (3 mL). The reaction mixture was filtered, the precipitate re-dissolved in ethyl acetate and extracted with NaOH (2M), dried over MgSO4, filtered and evaporated.
Yield: 16%
1H NMR (D6-DMSO): 0.92 (s, 9H); 3.13 (d, 2H); 7.30 (d, 1H); 7.58 (d, 1H); 7.98 (d, 2H); 8.16 (d, 2H); 8.52 (t, 1H); 12.76 (br s, 1H).
4-Amino benzoic acid (36 mmol) was suspended in 1,2-dichloroethane. Pyridine (44 mmol) was added followed by drop wise addition of 3,3-dimethyl-butyryl chloride (44 mmol). The mixture was stirred over night at ambient temperature, and then filtered. The solids were washed with 1,2-dichloroethane and dried in vacuo.
Yield: 55%
1H NMR (D6-DMSO): 1.02 (t, 9H); 2.22 (s, 2H); 7.71 (d, 2H); 7.88 (d, 2H); 10.09 (s, 1H); 12.68 (br, 1H).
4-(3,3-Dimethyl-butyrylamino)-benzoic acid (2 mmol) was dissolved in DMF (5 mL), N-chlorosuccinimide (8.5 mmol) was added portion wise. The reaction mixture was stirred at 40° C. over night. Another 8.5 mmol N-chlorosuccinimide was added, the reaction mixture was then stirred over night at 50° C. Another 4.3 mmol N-chloro-succinimide was added, stirring was continued at 50° C. for 2 h. This was repeated 5 times. Water (30 mL) was added, the formed preciptate was filtered off, washed with water and dried in vacuo.
Yield: 82%
1H NMR (D6-DMSO): 1.07 (s, 9H); 2.26 (s, 2H); 7.95 (s, 2H); 9.91 (s, 1H).
3,5-Dichloro-4-(3,3-dimethyl-butyrylamino)-benzoic acid (1.7 mmol) was suspended in 1,2-dichloroethane (15 mL) and DMF (150 μL) under an argon atmosphere. Oxalylchloride (2M in dichloromethane, 1.02 mL) was added slowly to the stirred suspension. After stirring at room temperature for 1 h the solvent was removed by evaporation under reduced pressure, and the reaction mixture was re-dissolved in 1,2-dichloroethane (15 mL). A suspension of 2-amino thiazole (1.7 mmol) and pyridine (1.7 mmol) in 1,2-dichloroethane (5 mL) was added portion wise. The reaction mixture was stirred at 50° C. over night. The reaction mixture evaporated and re-dissolved in ethyl acetate, then washed with NaOH (0.1M). The organic phase was dried over MgSO4, filtered and evaporated. The crude was re-crystallized from EtOH.
Yield: 7% (3% overall)
1H NMR (D6-DMSO): 1.08 (s, 9H); 2.27 (s, 2H); 7.31 (d, 1H); 7.58 (d, 1H); 8.20 (s, 1H); 9.92 (s, 1H); 12.87 (br s, 1H).
4-Amino-N-thiazol-2-yl-benzamide (0.46 mmol) was suspended in 1,2-dichloroethane (5 mL) and 2-isocyanato-2-methyl-propane (2.3 mmol) was added. The reaction mixture was heated at 140° C. by microwave irradiation for 3.5 h. The reaction mixture was evaporated to dryness and purified by preparative HPLC-MS.
Yield: 11%
LC/MS (m/z) 372 (MH+); RT=2.21 (method A); purity (UV, ELSD): 95%, 98%.
133: [4-(Thiazol-2-ylcarbamoyl)-phenyl]-carbamic acid 2,2-dimethyl-propyl ester 4-Amino-N-thiazol-2-yl-benzamide (0.46 mmol) was suspended in 1,2-dichloroethane (5 mL) and DIPEA (2.3 mmol) and 2,2-dimethylpropyl chloroformate (0.46 mmol) was added. The reaction mixture was stirred at 50° C. for 24 h and 70° C. for 24 h. The crude mixture was evaporated to dryness and purified by preparative HPLC-MS.
Yield: 28%
1H NMR (D6-DMSO): 0.96 (s, 9H); 3.84 (s, 2H); 7.26 (d, 1H); 7.55 (d, 1H); 7.62 (d, 2H); 8.06 (d, 2H); 10.00 (s, 1H); 12.44 (br, 1H).
Pharmacological Testing
The compounds of the invention were tested according to the following methods:
A2A Efficacy Assays
Cloning of the human cDNA encoding the A2a receptor.
cDNA was obtained by random primed reverse transcription of human fetal brain RNA (Clonetech). A subsequent polymerase chain reaction (PCR) was performed using the cDNA as template and the oligonucleotides TTTACGCGTGGCCATGCCCATCATGGGCTCCTC and TTTCTAGAATCAGGACACTCCTGCTCCATC as primers for the amplification. The amplification was performed using Pfu polymerase (Stratagene, in accordance with the manufactures recommendation) with an annealing temperature of 54° C. The reaction mixture was analyzed by an agarose gel electrophoresis and a band of 1.2 kb was excised and the DNA eluded. The eluded DNA was digested with the restriction enzymes MluI and XbaI and ligated into a vector, pCIneo, cut with the same enzymes. DNA was isolated and sequenced. CHO cells was transfected with the pCIneo clone expressing the A2a receptor and cells with stable integration of the plasmids were isolated after 2-3 weeks growth in the presence of either 5 mg/ml or 10 mg/ml G418.
CHO cells transfected with A2A receptors as described above were grown in F12 nutrient mixture (kaighs modification, Life technologies) with 10% FCS, 1% glutamin and 1% penicillin/streptomycin and 1 mg/mL G418.
24 h prior to assay performance, 10000 cells/well were seeded in costar 96-well plates in media without G418 to 60-80% confluence. The cells were stimulated with NECA (00-9498, final concentration 75 nM) corresponding to about 80% agonist efficacy.
The cell media was removed and the cells washed 3 times in 37° C. pre-equilibrated PBS and incubated (on shaker) with 10 μL of a suspension of acceptor beads and 10 μL of a solution of test compound or standard compound (0-10 μM) in darkness for 30 min at 25° C. before addition of 30 μl of a suspension of donor beads and further incubation 60-120 min in darkness. The plates were analysed according to manufacturers instruction (Alpha screen, Perkin Elmer (Pachard Biosciense)).
The acceptor beads were suspended in a stimulation buffer (5 mM HEPES, 0.1% BSA in Hanks balanced salt pH 7.4 w/o phenol red (Gibco). The donor beads were suspended in a lysis buffer (the stimulation buffer with 0,3% Tween 20 and biotinylated cAMP) according to manufacturers instruction (Alpha screen, Perkin Elmer (Pachard Biosciense)).
The data were fitted with non-linear regression, and IC50 and Ki values were calculated from the equations:
IC50=([I]/(100/(100-% INH))/(1+([ag]/EC50)
and
Ki=IC50/(1−[ag]/EC50),
where [I] is the inhibitor concentration, [ag] is the assay agonist concentration and EC50 is the agonist concentration required for half maximal effect.
A2A Binding Assay:
Membrane Preparations for A2A Binding Analysis:
Expression in Insect Cells
The human A2a encoding DNA were excised from the pCIneo constructs by MluI and XbaI and subcloned into the pFASTBAC2 vector cut with XbaI and BssHII. The inserts were recombined into the baculo vector using the Bac-to-Bac® system (Invitrogen). The generation and isolation of baculo virus was performed as described by the distributor (Invitrogen). High Five cells (Invitrogen) was grown at 27° C. in suspension to a density of 1*106 and infected with a MOI of 0.5. The cells are harvested 72 h post infection and membranes prepared.
High five cells expressing A2A receptors were homogenized in 50 mM tris-buffer pH 7.4 in an ultra Turrax homogenisator. The membranes were diluted to a concentration of 0.6 mg/ml and 2U Adenosine deaminase (Roche)/ml membrane suspension was added. The solution was preincubated 30 min at 37° C. before use.
A2A Binding Analysis:
Binding assay was performed in 96 well flat bottom plate and initiated by mixing 10.6 μg protein/well with solutions of standard compounds or test compounds (final concentrations 0-10 μM) and 1 nM final concentration of 3H-ZM241385 (R1036 from Tocris). All test compounds were diluted in 50 nM trisbuffer from DMSO-stocks (2 mM or 10 mM). The reactions (final volume=200 μL) were incubated for 30 min at 25° C. and washed on Unifilter-GF/B with water. The filters were dried 20 min (37° C.) before addition of 35 μl Microscient-0 or Optiphase supermix and counting in a Trilux counter for 1 min.
The data were fitted with non-linear regression, and IC50 and Ki values were calculated from the equations:
IC50=([I]/(100/(100-% INH))/(1+([L]/KD)
and
Ki=IC50/(1−[L]/KD),
where [I] is the inhibitor concentration, and [L] and KD are concentration and dissociation equilibrium constant of the radiotracer, respectively.
The exemplified compounds 1-119 of the invention are A2A receptors antagonists having a human A2A binding affinity (Ki) of 530 nM or less.
The pharmaceutical formulations of the invention may be prepared by conventional methods in the art.
For example: Tablets may be prepared by mixing the active ingredient with ordinary adjuvants and/or diluents and subsequently compressing the mixture in a conventional tabletting machine. Examples of adjuvants or diluents comprise: Corn starch, potato starch, talcum, magnesium stearate, gelatine, lactose, gums, and the like. Any other adjuvants or additives usually used for such purposes such as colourings, flavourings, preservatives etc. may be used provided that they are compatible with the active ingredients.
Solutions for injections may be prepared by dissolving the active ingredient and possible additives in a part of the solvent for injection, preferably sterile water, adjusting the solution to the desired volume, sterilising the solution and filling it in suitable ampoules or vials. Any suitable additive conventionally used in the art may be added, such as tonicity agents, preservatives, antioxidants, etc.
Typical examples of recipes for the formulation of the invention are as follows:
1) Tablets containing 5.0 mg of a compound of the invention calculated as the free base:
2) Tablets containing 0.5 mg of a compound of the invention calculated as the free base:
3) Syrup containing per millilitre:
4) Solution for injection containing per millilitre:
| Number | Date | Country | Kind |
|---|---|---|---|
| PA200301579 | Oct 2003 | DK | national |
| PA200400229 | Feb 2004 | DK | national |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/DK04/00733 | Oct 2004 | US |
| Child | 11312661 | Dec 2005 | US |
